Auxiliary tool and consumable device for anti-pollution detection card

Abstract

The utility model provides an auxiliary tool for an anti-pollution detection card, which comprises a liquid guide bin connected with the anti-pollution detection card, a PCR (Polymerase Chain Reaction) test tube arranged in the liquid guide bin and used for accommodating amplification products, and a cutting mechanism, the cutting mechanism comprises a cutting table arranged in the liquid guide bin, a cutting groove formed in the cutting table and matched with the PCR test tube, and a cutting knife mounted in the cutting groove through a floating plug. According to the utility model, the integral sealing layout is adopted, so that the problem of repeated cover opening can be effectively avoided, the possibility of pollution is reduced, and the accuracy of a test result is further ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid isothermal amplification, in particular to an auxiliary tool for an anti-pollution detection card and a detection consumable device. Background Art

[0002] Recombinase Polymerase Amplification (RPA) is a new technology used for DNA amplification in molecular biology. It can achieve reaction amplification under constant temperature conditions and obtain amplified products within 20 minutes. It is more suitable for portable equipment or field conditions and is considered to be a potential alternative to the traditional detection method of polymerase chain reaction (PCR).

[0003] At present, false positives caused by RPA are still a problem that needs attention. The existing technology needs to open the lid repeatedly during operation, which is prone to cross contamination or contact with the outside air, which is very likely to cause aerosol contamination. Therefore, the use process cannot ensure the accuracy of the test results. Based on this, how to facilitate the operation to meet the needs of rapid on-site testing and avoid opening the lid to reduce the possibility of contamination in the amplification reaction is a technical problem to be solved by the utility model.

[0004] Therefore, it is desired in the art to provide an auxiliary tool for an anti-contamination test card and a test consumable device to solve the above technical problems. Utility Model Content

[0005] The purpose of the utility model is to provide an auxiliary tool for an anti-contamination test card, which not only mixes the amplification product with the calibration liquid to form a mixed solution by mechanical pushing, but also allows the mixed solution to smoothly contact the test paper by being installed on the anti-contamination test card, so as to interpret the result through the change of the test paper. In this way, the problem of repeated opening of the cover can be effectively avoided, thereby effectively reducing the possibility of contamination, so as to further ensure the accuracy of the test results. In addition, a detection consumable device is also provided.

[0006] According to a first aspect of the utility model, an auxiliary tool for an anti-contamination test card is provided, comprising a liquid guide chamber connected to the anti-contamination test card, wherein a PCR test tube containing an amplification product is arranged in the liquid guide chamber, and

[0007] The cutting mechanism includes a cutting platform arranged in the liquid guiding chamber, a cutting groove arranged on the cutting platform and adapted to the PCR test tube, and a cutting knife installed in the cutting groove through a floating plug.

[0008] In one embodiment, the cutting groove is configured as a circular through groove, and the length of the cutting knife is equal to the diameter of the cutting groove.

[0009] In one embodiment, the floating plug is configured as a cylindrical structure, and the diameter of the floating plug is not greater than the diameter of the cutting groove, so as to allow the floating plug to be nested in the cutting groove.

[0010] In one embodiment, the cutting table is configured as a cylindrical structure, and the outer circumferential surface of the cutting table forms a sealed connection with the inner circumferential surface of the liquid conducting chamber to divide the liquid conducting chamber into an upper chamber containing the PCR test tube and a lower chamber containing the calibration liquid.

[0011] In one embodiment, the cutter is configured as a triangular prism structure and comprises a cutting surface for breaking the PCR tube and a supporting surface abutting against the floating plug.

[0012] In one embodiment, a step extending radially inwardly and used to abut against the header is formed on the inner wall of the liquid guiding bin.

[0013] In one embodiment, a guide groove for communicating with the anti-pollution detection card is provided at the bottom of the liquid guide bin.

[0014] The auxiliary tool further includes a closing bead nested in the guide groove and used for closing the lower chamber.

[0015] In one embodiment, the auxiliary tool also includes a compartment cover nested outside the liquid guide compartment and used to drive the PCR test tube downward.

[0016] According to a second aspect of the utility model, a consumable device is provided, comprising an anti-pollution detection card, a test paper laid flat in the anti-pollution detection card, and an auxiliary tool as described above adapted to the anti-pollution detection card.

[0017] In one embodiment, a pin for embedding into the guide groove is provided at the upper end surface of the anti-pollution detection card.

[0018] The ejector pin is configured to drive the sealing bead upward and allow the mixed liquid to flow toward the test paper.

[0019] Compared with the prior art, the advantages of the utility model are:

[0020] First, the overall sealing layout of the utility model not only allows the amplification product to mix with the calibration liquid to form a mixed liquid by mechanical pushing, but also allows the mixed liquid to smoothly contact the test paper by installing it on the anti-pollution detection card 200, so as to interpret the results through the changes in the test paper. In this way, the problem of repeated opening of the cover can be effectively avoided, thereby effectively reducing the possibility of contamination, and further ensuring the accuracy of the test results.

[0021] Secondly, since the anti-pollution detection card is constructed as a closed transparent card shell, the device can realize an on-site visual detection mode, thereby avoiding contact with air and facilitating observation of experimental results.

[0022] Third, the utility model is suitable for RPA on-site detection, and can ensure that the RPA amplification product will not come into contact with the outside air during use, avoiding aerosol contamination of the reaction product caused by opening the cover during amplification and sample addition. In addition, the device is small in size, convenient for personnel to operate and carry, and effectively meets the needs of rapid on-site detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The present invention will be described in detail below in conjunction with the accompanying drawings, in which:

[0024] Figure 1 The structure of the auxiliary tool for the anti-pollution detection card according to the utility model is schematically shown;

[0025] Figure 2 It is a partial schematic diagram of an auxiliary tool for an anti-pollution detection card according to the utility model;

[0026] Figure 3 The structure of the consumable device according to the utility model is schematically shown.

[0027] In the drawings, the same reference numerals are used for the same components. The drawings are not drawn to scale. DETAILED DESCRIPTION

[0028] In order to make the technical solutions and advantages of the present invention more clearly understood, the exemplary embodiments of the present invention are further described in detail below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than an exhaustive list of all embodiments. In addition, the embodiments of the present invention and the features in the embodiments can be combined with each other if there is no conflict.

[0029] The utility model will be further described below in conjunction with the accompanying drawings.

[0030] Figure 1 The structure of the auxiliary tool 100 for the anti-contamination detection card according to the utility model is schematically shown;

[0031] Figure 2 It is a partial schematic diagram of an auxiliary tool 100 for an anti-pollution detection card according to the utility model;

[0032] Figure 3 The structure of the consumable device according to the utility model is schematically shown.

[0033] like Figures 1 to 3 As shown, according to the first aspect of the present invention, an auxiliary tool 100 for an anti-contamination test card is provided, which includes a liquid guide chamber 10 for connecting to an anti-contamination test card 200. Preferably, a PCR test tube 11 for accommodating amplification products is provided in the liquid guide chamber 10.

[0034] According to the utility model, if Figure 1 and 2 As shown, the auxiliary tool 100 for the anti-contamination detection card also includes a cutting mechanism. Preferably, the cutting mechanism includes a cutting table 12 disposed in the liquid guide chamber 10, a cutting groove 13 disposed on the cutting table 12 and adapted to the PCR test tube 11, and a cutting knife 14 installed in the cutting groove 13 through a floating plug 18. Preferably, the PCR test tube 11 can descend and move to the cutting groove 13, so that it is broken by the cutting knife 14 to release the amplification product.

[0035] According to the utility model, if Figure 1 and 2 As shown, the auxiliary tool 100 for the anti-contamination test card also includes a compartment cover 20 nested outside the liquid guide compartment 10 and used to drive the PCR test tube 11 downward. Preferably, the overall height of the compartment cover 20 and the liquid guide compartment 10 is about 22 mm, which is consistent with the height of the PCR test tube 11.

[0036] In the present invention, by pressing down the compartment cover 20, the compartment cover 20 moves relative to the liquid guide compartment 10, so that the PCR test tube 11 moves downward in the liquid guide compartment 10, and the bottom of the PCR test tube 11 is broken by the cutting knife 14, so that the amplification product in the PCR test tube 11 flows out smoothly.

[0037] In one embodiment, the cutting groove 13 is configured as a circular through groove. Preferably, the length of the cutting knife 14 is equal to the diameter of the cutting groove 13. In this way, the PCR test tube 11 after descending can be fully contacted with the cutting knife 14 to smoothly break the PCR test tube 11, thereby allowing the amplification product to flow out.

[0038] In the utility model, the height of the compartment cover 20 is slightly higher than the height of the cutting groove 13, and the height of the cutting groove 13 is equal to the total height of the cutting knife 14 and the float 18. Therefore, pressing the compartment cover 20 downward can not only break the PCR test tube 11 through the cutting knife 14, but also push the float 18 out of the cutting groove 13.

[0039] In one embodiment, the float 18 is configured as a cylindrical structure. Preferably, the diameter of the float 18 is not greater than the diameter of the cutting groove 13, so that the float 18 can be nested in the cutting groove 13. Preferably, the float 18 is made of rubber material, and the float 18 can prevent the cutter 14 from contacting the calibration fluid (described below), thereby preventing the cutter 14 from rusting and affecting the detection result.

[0040] In this way, the float 18 can be firmly installed in the cutting groove 13, thereby ensuring the stability of the float 18 during the cutting process, further ensuring that the PCR tube 11 can be fully cut, and then ensuring that the amplification product in the PCR tube 11 can flow out completely, which helps to improve the accuracy of subsequent detection data.

[0041] In the present invention, after the PCR test tube 11 is completely broken by the cutting knife 14, it continues to move downward until the float plug 18 is pushed out from the cutting groove 13, thereby allowing the amplification product to flow downward to mix with the calibration solution.

[0042] In one embodiment, the header 12 is configured as a cylindrical structure. Preferably, the outer circumferential surface of the header 12 forms a sealed connection with the inner circumferential surface of the liquid guiding chamber 10 , so that the liquid guiding chamber 10 can be divided into an upper chamber 101 and a lower chamber 102 .

[0043] In the present utility model, if Figure 1 and 2 As shown, a PCR test tube 11 is contained in the upper chamber 101 of the liquid guide chamber 10. Preferably, the PCR test tube 11 is arranged in the upper chamber 101 along the longitudinal direction, and the PCR test tube 11 is axially abutted against the chamber cover 20, so that the PCR test tube 11 can more easily descend after being pressed and smoothly enter the cutting groove 13.

[0044] In the present invention, the calibration liquid is contained in the lower chamber 102 of the liquid guide chamber 10. Preferably, the amplification product can flow to the lower chamber 102 through the cutting groove 13, thereby forming a mixed liquid with the calibration liquid for subsequent detection work.

[0045] In a preferred embodiment, the upper end of the cutting platform 12 is arranged to be inclined toward the cutting groove 13 , so that the amplification product can be guided into the cutting groove 13 more easily.

[0046] In one embodiment, Figure 1 and 2As shown, the cutter 14 is configured as a triangular prism structure. Preferably, the cutter 14 includes a cutting surface 141 and a supporting surface 142. The cutting surface 141 is configured as a sharp surface, so that when it contacts the bottom of the PCR test tube 11, it can effectively cut to quickly release the amplification product into the upper chamber 101; the supporting surface 142 is configured as a plane, so that it can have a larger contact area with the float 18 to improve the stability of the cutter 14 during the cutting process.

[0047] In addition, since the cutter 14 abuts against the float 18 via the support surface 142 , the float 18 can be more easily pushed out of the cutting groove 13 during the pressing process, thereby allowing the amplification product in the upper chamber 101 to flow into the lower chamber 102 .

[0048] In one embodiment, Figure 1 and 2 As shown, a step 15 extending radially inward is formed on the inner wall of the liquid guide chamber 10. Preferably, the cutter 12 is axially abutted against the step 15, thereby effectively limiting the position of the cutter 12 in the liquid guide chamber 10 to further improve the success rate of breaking the PCR tube 11 to release the amplification product.

[0049] In one embodiment, Figure 1 and 2 As shown, a guide groove 16 is provided at the bottom of the liquid guide chamber 10. Preferably, the guide groove 16 can be connected to the anti-contamination detection card 200, so as to allow the mixed solution formed by the amplification product and the calibration solution to flow into the anti-contamination detection card 200.

[0050] According to the utility model, if Figure 1 and 2 As shown, the auxiliary tool 100 for the anti-contamination test card further includes a sealing bead 17 nested in the guide groove 16. Preferably, the sealing bead 17 can block the lower chamber 102 of the liquid guide chamber 10 in the initial state, thereby ensuring that the amplification product and the calibration liquid can be fully mixed.

[0051] like Figure 3 As shown, according to the second aspect of the utility model, a consumable device is provided, including an anti-pollution detection card 200, a test paper 210 arranged flat in the anti-pollution detection card 200, and an auxiliary tool 100 as described above adapted to the anti-pollution detection card 200.

[0052] Preferably, the anti-pollution detection card 200 is constructed as a closed transparent card shell made of plastic.

[0053] In one embodiment, Figure 3As shown, a pin 201 for embedding into the guide groove 16 is provided at the upper end surface of the anti-contamination detection card 200. Preferably, the pin 201 is configured to drive the blocking beads 17 upward and allow the mixed solution formed by the amplification product and the calibration solution to flow into the anti-contamination detection card 200, thereby contacting the test paper 210 and changing the result of the interpretation through the test paper 210.

[0054] The utility model can ensure that the reagents will not diffuse into the outside air during the amplification reaction, and because the problem of repeated opening of the cover is avoided, the possibility of contamination is effectively reduced to further ensure the accuracy of the test results. In addition, the device is small in size, convenient for personnel to operate and carry, and effectively meets the needs of on-site rapid detection.

[0055] Preferably, the consumable device of the utility model is an on-site detection auxiliary device based on RPA. During use, the device can ensure that the RPA amplification product does not come into contact with the outside air, and avoid aerosol contamination of the reaction product caused by opening the cover during amplification and sample addition.

[0056] Combination Figures 1 to 3 The working principle of the utility model is introduced below:

[0057] First, open the chamber cover 20 and place the PCR test tube 11 containing the amplification product in the guide liquid chamber 10;

[0058] Then, the compartment cover 20 is closed and pressed downward to make the compartment cover 20 move downward relative to the liquid conducting compartment 10 , and the PCR test tube 11 also moves downward in the liquid conducting compartment 10 .

[0059] During this process, first, the bottom of the PCR test tube 11 is broken by the cutting knife 14, thereby allowing the amplified product to flow outward; then, it continues to descend and pushes the float 18 out of the cutting groove 13, thereby allowing the amplified product to flow into the lower chamber 102 of the liquid guide tank 10 and form a mixed liquid with the calibration liquid.

[0060] Thereafter, the auxiliary tool 100 is mounted on the anti-contamination detection card 200 .

[0061] During this process, the ejector pin 201 of the anti-pollution detection card 200 drives the sealing bead 17 upward, thereby causing the lower chamber 102 to communicate with the anti-pollution detection card 200, allowing the mixed liquid to flow into the anti-pollution detection card 200 and contact the test paper 210, thereby changing the test paper 210 to read the result.

[0062] Compared with the prior art, the advantages of the present invention are:

[0063] First, the overall sealing layout of the utility model not only allows the amplification product to mix with the calibration liquid to form a mixed solution by mechanical pushing, but also allows the mixed solution to smoothly contact the test paper 210 by being installed on the anti-pollution detection card 200, so as to read the result through the change of the test paper 210. In this way, the problem of repeated opening of the cover can be effectively avoided, thereby effectively reducing the possibility of contamination, and further ensuring the accuracy of the test results.

[0064] Secondly, since the anti-pollution detection card 200 is constructed as a closed transparent card shell, the device can realize an on-site visual detection mode, thereby avoiding contact with air and facilitating observation of experimental results.

[0065] Third, the utility model is suitable for RPA on-site detection, and can ensure that the RPA amplification product does not come into contact with the outside air during use, avoiding aerosol contamination of the reaction product caused by opening the cover during amplification and sample addition. In addition, the device is small in size, convenient for personnel to operate and carry, and effectively meets the needs of on-site rapid detection.

[0066] In the description of the present utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features.

[0067] In the present utility model, unless otherwise clearly stipulated and limited, the terms such as "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal connection of two elements.

[0068] For those skilled in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0069] The above are only preferred embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art can easily make changes or modifications within the scope of the present invention, and such changes or modifications should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. An auxiliary tool for an anti-pollution detection card, characterized in that: include: A liquid conducting chamber (10) for connecting to an anti-pollution detection card (200), wherein a PCR test tube (11) for accommodating amplification products is arranged in the liquid conducting chamber (10); and A cutting mechanism, the cutting mechanism comprising a cutting table (12) arranged in the liquid guide chamber (10), a cutting groove (13) arranged on the cutting table (12) and adapted to the PCR test tube (11), and a cutting knife (14) installed in the cutting groove (13) via a floating plug (18).

2. The auxiliary tool for the anti-pollution detection card according to claim 1, characterized in that: The cutting groove (13) is configured as a circular through groove, and the length of the cutting knife (14) is equal to the diameter of the cutting groove (13).

3. The auxiliary tool for the anti-pollution detection card according to claim 2, characterized in that: The floating plug (18) is configured as a cylindrical structure, and the diameter of the floating plug (18) is not greater than the diameter of the cutting groove (13), so as to allow the floating plug (18) to be nested in the cutting groove (13).

4. The auxiliary tool for the anti-pollution detection card according to claim 3, characterized in that: The cutting table (12) is configured as a cylindrical structure, and the outer peripheral surface of the cutting table (12) forms a sealed connection with the inner peripheral surface of the liquid guide chamber (10) to divide the liquid guide chamber (10) into an upper chamber (101) containing the PCR test tube (11) and a lower chamber (102) containing a calibration liquid.

5. The auxiliary tool for the anti-pollution detection card according to claim 4, characterized in that: The cutting knife (14) is configured as a triangular prism structure and comprises a cutting surface (141) for breaking the PCR test tube (11) and a supporting surface (142) abutting against the floating plug (18).

6. The auxiliary tool for the anti-pollution detection card according to claim 5, characterized in that: A step (15) is formed on the inner wall of the liquid guide chamber (10) and extends radially inwards and is used to abut against the header (12).

7. The auxiliary tool for the anti-pollution detection card according to claim 6, characterized in that: A guide groove (16) for communicating with the anti-pollution detection card (200) is provided at the bottom of the liquid guide bin (10). The auxiliary tool also includes a closing bead (17) nested in the guide groove (16) and used to seal the lower chamber (102).

8. The auxiliary tool for the anti-pollution detection card according to claim 7, characterized in that: The auxiliary tool also includes a chamber cover (20) which is nested outside the liquid guide chamber (10) and is used to drive the PCR test tube (11) downward.

9. A consumable device, characterized in that: It comprises an anti-pollution detection card (200), a test paper (210) arranged in a flat manner inside the anti-pollution detection card (200), and an auxiliary tool according to any one of claims 1 to 8 adapted to the anti-pollution detection card (200).

10. The consumable device according to claim 9, characterized in that: A ejector pin (201) for embedding into the guide groove (16) is provided at the upper end surface of the anti-pollution detection card (200). The ejector pin (201) is configured to drive the sealing bead (17) upward and allow the mixed liquid to flow toward the test paper (210).

Images