Arduino-based multichannel nucleic acid automatic extraction device

Abstract

The utility model relates to the technical field of nucleic acid extraction instruments, in particular to an Arduino-based multi-channel nucleic acid automatic extraction device which comprises a shell provided with a cavity; a switch group, a display module and an electrified film are arranged on the front end surface of the shell; a power module, a control module, an electromagnetic relay, a heating module and a control module are arranged on the upper end face of the shell; a fan group and an ultraviolet lamp are arranged on the rear end surface of the shell; and the cavity of the shell is provided with a movement module and a 96-pore plate. The device realizes multi-channel nucleic acid extraction with low cost and simple structure, and has the advantages of high extraction efficiency and short time period. In the large-scale nucleic acid extraction work, the device shows higher efficiency, portability and economical efficiency.

Description

Technical Field

[0001] The utility model relates to a multi-channel automatic nucleic acid extraction device. Background Art

[0002] In the prior art, nucleic acid extraction is mainly divided into manual extraction and machine extraction. Manual extraction requires well-trained technicians to operate, and errors may be introduced due to human factors, resulting in inaccurate results. At present, machine extraction mainly uses commercial instruments on the market, which are large in size, expensive, and complex in structure. In order to achieve low-cost multi-channel automatic nucleic acid extraction, we designed a small and economical device to complete the various steps of nucleic acid extraction. The device measures 250 mm * 200 mm * 380 mm and costs less than 500 yuan. It can complete the nucleic acid extraction of 16-channel samples within 21 minutes. It has the characteristics of high extraction efficiency and short extraction time cycle. It is more efficient, portable and economical in batch nucleic acid extraction. Summary of the invention

[0003] The utility model aims to provide a multi-channel automatic nucleic acid extraction device based on Arduino to solve the problems existing in the above-mentioned prior art.

[0004] The Arduino-based multi-channel automatic nucleic acid extraction device of the utility model comprises a shell (110) provided with a cavity; the front end surface of the shell (110) is provided with a switch group (105), a display module (106) and a power-on film (109); the upper end surface of the shell (110) is provided with a power module (101), an electromagnetic relay (102), a heating module (103) and a control module (104); the rear end surface of the shell (110) is provided with a fan group (111) and an ultraviolet lamp (112); and the cavity of the shell (110) is provided with a motion module (107) and a 96-well plate (108).

[0005] Optionally, the motion module (107) comprises a first motor (1071), a second motor (1072), a third motor (1073), a magnetic rod (1074), a vibration module (1075) and a magnetic rod sleeve (1076); the electromagnetic relay (102) is used to control the vibration of the vibration module; the first motor (1071) is used to drive the motion module (107) to perform lateral movement; the second motor (1072) is used to drive the magnetic rod sleeve (1076) to perform longitudinal movement; and the third motor (1073) is used to drive the magnetic rod (1074) to perform longitudinal movement.

[0006] Optionally, the switch group (105) comprises a first switch (1051), a second switch (1052) and a third switch (1053); the first switch (1051) is used to control the power module (101); the second switch (1052) is used to control the fan group (111); and the third switch (1053) is used to control the ultraviolet lamp (112).

[0007] Optionally, the power supply module (101) provides a working voltage for the control module (104); the control module (104) is used to control the heating module (103), the first motor (1071), the second motor (1072), the third motor (1073), the display module (106), the power film (109), the fan group (111) and the ultraviolet lamp (112).

[0008] Optionally, the heating module (103) is used to heat the 96-well plate (108).

[0009] Optionally, the fan group (111) includes a first fan (1111) and a second fan (1112); the air intake direction of the first fan (1111) is opposite to the air intake direction of the second fan (1112), and is used for exchanging internal air and external air of the multi-channel automatic nucleic acid extraction device.

[0010] Optionally, the specific model of the control module (104) is Arduino Uno.

[0011] Optionally, the display module (106) is specifically a USART serial port screen.

[0012] The utility model discloses an Arduino-based multi-channel automatic nucleic acid extraction device, which can realize low-cost multi-channel automatic nucleic acid extraction and has the characteristics of high extraction efficiency, short extraction time cycle, etc., and is more portable and economical in batch nucleic acid extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a structural schematic diagram of the multi-channel automatic nucleic acid extraction device described in the utility model.

[0014] Figure 2 It is a schematic diagram of the motion module structure of the multi-channel automatic nucleic acid extraction device in the utility model.

[0015] Figure 3 It is a rear view of the multi-channel automatic nucleic acid extraction device of the present invention.

[0016] Figure 4 It is a schematic diagram of the circuit principle of the multi-channel automatic nucleic acid extraction device in the utility model.

[0017] Figure 5 It is a comparison chart of the DNA yields of the multi-channel automatic nucleic acid extraction device described in the utility model and manual extraction.

[0018] Reference numerals: power module (101), electromagnetic relay (102), heating module (103), control module (104), switch group (105), first switch (1051), second switch (1052), third switch (1053), display module (106), motion module (107), first motor (1071), second motor (1072), third motor (1073), magnetic rod (1074), vibration module (1075), magnetic rod sleeve (1076), 96-well plate (108), energized film (109), fan group (111), first fan (1111), second fan (1112), ultraviolet lamp (112). DETAILED DESCRIPTION

[0019] For those skilled in the art, various other corresponding changes and deformations can be made according to the technical solutions and concepts described above, and all of these changes and deformations should fall within the protection scope of the claims of the utility model.

[0020] Arduino Uno is an open source, single-board microcontroller that is widely used in a variety of electronic projects and prototyping. It was designed by the Arduino development team in Italy and comes with a range of digital and analog input / output pins for connecting to a variety of sensors, actuators, and other peripherals.

[0021] USART serial port screen usually refers to a display screen with a USART serial communication interface, which is used to communicate and exchange data with a control unit or other devices.

[0022] like Figures 1 to 3As shown, the Arduino-based multi-channel automatic nucleic acid extraction device described in the utility model comprises: a housing (110) provided with a cavity; the front end surface of the housing (110) is provided with a switch group (105), a display module (106) and a power-on film (109); the upper end surface of the housing (110) is provided with a power module (101), an electromagnetic relay (102), a heating module (103) and a control module (104); the rear end surface of the housing (110) is provided with a fan group (111) and an ultraviolet lamp (112); and the cavity of the housing (110) is provided with a motion module (107) and a 96-well plate (108). The motion module (107) comprises a first motor (1071), a second motor (1072), a third motor (1073), a magnetic rod (1074), a vibration module (1075) and a magnetic rod sleeve (1076); the electromagnetic relay (102) is used to control the vibration of the vibration module; the first motor (1071) is used to drive the motion module (107) to move horizontally; the second motor (1072) is used to drive the magnetic rod sleeve (1076) to move longitudinally; the third motor (1073) is used to drive the magnetic rod (1074) to move longitudinally. The switch group (105) comprises a first switch (1051), a second switch (1052) and a third switch (1053); the first switch (1051) is used to control the power module (101); the second switch (1052) is used to control the fan group (111); and the third switch (1053) is used to control the ultraviolet lamp (112). The heating module (103) is used to heat the 96-well plate (108). The fan group (110) comprises a first fan (1111) and a second fan (1112); the air intake direction of the first fan (1111) is opposite to that of the second fan (1112), and is used for exchanging internal air and external air of the multi-channel nucleic acid automatic extraction device.

[0023] like Figure 4 As shown, Figure 4 The present invention is a schematic diagram of the circuit principle of the multi-channel automatic nucleic acid extraction device. The power module (101) provides the control module (104) with a working voltage; the control module (104) is used to control the heating module (103), the first motor (1071), the second motor (1072), the third motor (1073), the display module (106), the power film (109), the fan group (111) and the ultraviolet lamp (112).

[0024] The specific model of the control module (104) is Arduino Uno.

[0025] The display module (106) is specifically a USART serial port screen.

[0026] The working process of the multi-channel automatic nucleic acid extraction device based on Arduino described in the utility model is as follows, and the extraction of calf thymus DNA by a 16-channel nucleic acid extraction device is used as an example for explanation.

[0027] S101, turning on the first switch (1051), so that the power module (101) provides a working voltage for the control module (104).

[0028] S102, insert the magnetic rod sleeve (1076).

[0029] S103. Place the pre-prepared sample in a 96-well plate (108), wherein the pre-prepared sample includes: 400 μL magnetic beads, 800 μL binding solution, 300 μL 70% ethanol, and 100 μL elution solution.

[0030] S104, setting the time of each step on the display module (106), wherein each step includes: magnetic bead adsorption, DNA adsorption, first washing, second washing and DNA elution.

[0031] S105, after taking out the 96-well plate (108), turn on the third switch (1053), and the ultraviolet lamp (112) starts sterilization.

[0032] like Figure 5 As shown, Figure 5 This is a comparison chart of the DNA yield of the multi-channel automatic nucleic acid extraction device and manual extraction in the utility model. Specifically, we use the same method to extract calf thymus DNA, and it can be seen that the DNA yield of the 16-channel nucleic acid extraction device is greater than the yield of manual extraction.

[0033] As shown in Table 1, Table 1 is a comparison of the multi-channel automatic nucleic acid extraction device and manual extraction time in the utility model. Specifically, we use the same method to extract calf thymus DNA. It can be seen that it takes 30.75 minutes to complete one nucleic acid extraction manually, while the multi-channel extraction device can complete the nucleic acid extraction of 16 samples in 20.75 minutes, which significantly shortens the extraction time and improves work efficiency.

[0034] step Manual extraction time (s) 16-channel extraction device extraction time Magnetic bead adsorption 15 15 Adsorption 600 300 Cleaning 1 15 15 Cleaning 2 15 15 dry 600 300 Elution 600 600 total 1845 1245

[0035] Table 1

[0036] The advantage of the Arduino-based multi-channel automatic nucleic acid extraction device described in the utility model is that it can complete multi-channel nucleic acid extraction with a low-cost and simple structure. The size of the device is 250 mm * 200 mm * 380 mm, the cost is less than 500 yuan, and the nucleic acid extraction of 16 channels can be completed within 21 minutes. Manual extraction completes the nucleic acid extraction of 1 sample within 31 minutes. Therefore, the designed multi-channel nucleic acid extraction device has the characteristics of high extraction efficiency and short extraction time cycle, and is more efficient, portable and economical in batch nucleic acid extraction.

Claims

1. A multi-channel automatic nucleic acid extraction device based on Arduino, characterized in that: include: A shell (110) is provided with a cavity; a switch group (105), a display module (106) and a power-on film (109) are provided on the front end surface of the shell (110); a power module (101), an electromagnetic relay (102), a heating module (103) and a control module (104) are provided on the upper end surface of the shell (110); a fan group (111) and an ultraviolet lamp (112) are provided on the rear end surface of the shell (110); and a motion module (107) and a 96-well plate (108) are provided in the cavity of the shell (110).

2. According to claim 1, a multi-channel automatic nucleic acid extraction device based on Arduino, characterized in that: The motion module (107) comprises a first motor (1071), a second motor (1072), a third motor (1073), a magnetic rod (1074), a vibration module (1075) and a magnetic rod sleeve (1076); the electromagnetic relay (102) is used to control the vibration of the vibration module; the first motor (1071) is used to drive the motion module (107) to perform lateral movement; the second motor (1072) is used to drive the magnetic rod sleeve (1076) to perform longitudinal movement; and the third motor (1073) is used to drive the magnetic rod (1074) to perform longitudinal movement.

3. According to the Arduino-based multi-channel automatic nucleic acid extraction device of claim 1, it is characterized in that: The switch group (105) comprises a first switch (1051), a second switch (1052) and a third switch (1053); the first switch (1051) is used to control the power module (101); the second switch (1052) is used to control the fan group (111); and the third switch (1053) is used to control the ultraviolet lamp (112).

4. According to claim 2, a multi-channel automatic nucleic acid extraction device based on Arduino, characterized in that: The power supply module (101) provides a working voltage for the control module (104); the control module (104) is used to control the heating module (103), the first motor (1071), the second motor (1072), the third motor (1073), the display module (106), the power film (109), the fan group (111) and the ultraviolet lamp (112).

5. According to the Arduino-based multi-channel automatic nucleic acid extraction device of claim 1, it is characterized in that: The heating module (103) is used to heat the 96-well plate (108).

6. According to the Arduino-based multi-channel automatic nucleic acid extraction device of claim 1, it is characterized in that: The fan group (111) comprises a first fan (1111) and a second fan (1112); the air intake direction of the first fan (1111) is opposite to that of the second fan (1112), and is used for exchanging internal air and external air of the multi-channel automatic nucleic acid extraction device.

7. According to claim 1, a multi-channel automatic nucleic acid extraction device based on Arduino, characterized in that: The specific model of the control module (104) is Arduino Uno.

8. According to the Arduino-based multi-channel automatic nucleic acid extraction device of claim 1, characterized in that: The display module (106) is specifically a USART serial port screen.

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