Animal tissue fixing device capable of automatically replacing formaldehyde

Abstract

The utility model relates to an animal tissue fixing device capable of automatically replacing formaldehyde, which belongs to the technical field of animal tissue treatment and comprises a sample storage tube, a fixing device used for fixing animal tissues is arranged in the sample storage tube, and a discharging device used for discharging fresh formaldehyde is arranged on the upper side of the sample storage tube. An extraction device for discharging waste liquid formaldehyde is arranged on the lower side of the sample storage tube. According to the animal tissue fixing device capable of automatically replacing formaldehyde, tissue is placed in an animal tissue positioning cylinder on the upper side of a net plate in a sample storage pipe and is soaked in formaldehyde, so that when formaldehyde flows into the sample storage pipe from a new liquid filling rubber hose channel, the formaldehyde can slowly move towards the side wall and flows to a flow guide cylinder through a flow guide cover; and after being used, the tissue is pumped away from a hole path below the sample storage tube, so that the tissue is prevented from being damaged by direct flushing and pollution, the tissue in the sample storage tube is effectively prevented from being polluted and damaged, and a cleaning and fixing method is mild.

Description

Technical Field

[0001] The utility model relates to the technical field of animal tissue processing, in particular to an animal tissue fixing device for automatically replacing formaldehyde. Background Art

[0002] Tissue sections have been widely used along with the development of microscopy technology and immunoassays. Paraffin sections preserve tissue structure well and have great medical value in pathology and retrospective studies. They are not only used to observe the morphological structure of normal cell tissues, but are also the main method used by pathology and forensic medicine to study, observe and judge the morphological changes of cell tissues. Tissues need to be fixed with a fixative or fixative solution. The commonly used fixative is formaldehyde. The morphological structure of tissues fixed with formaldehyde is well preserved, and they have strong penetrability and less tissue shrinkage.

[0003] Existing animal tissue experiments require the use of formaldehyde and replacement, but formaldehyde is very harmful to experimenters. When replacing formaldehyde for tissues, most laboratories use the original method of manually dumping formaldehyde waste liquid and injecting fresh formaldehyde with a syringe. In this process, formaldehyde drips and tissues slip, which poses a great health hazard to experimenters. Under these uncontrollable factors, we cannot estimate how much harm formaldehyde will cause to the body of experimenters during the experiment. For a long time, the experimental operation of replacing formaldehyde fixative has caused us great trouble and psychological pressure. At the same time, many students reported that they would have obvious physical discomfort for several consecutive days after contacting formaldehyde. Therefore, an animal tissue fixation device that automatically replaces formaldehyde is proposed to solve the above problems. Utility Model Content

[0004] In view of the deficiencies in the prior art, the utility model provides an animal tissue fixing device for automatically replacing formaldehyde, which has the advantages of not having to frequently change formaldehyde manually, being able to connect in series many tanks for processing tissues, and not having to manually take the tissues out during the tissue fixing process by the experimenter, and the tank design also protecting the tissues from pollution and damage, etc., which solves the problem that formaldehyde needs to be used and replaced in animal tissue experiments, but formaldehyde is very harmful to the experimenters, and most laboratories use the original method of manually dumping formaldehyde waste liquid and injecting fresh formaldehyde with a syringe when replacing formaldehyde for the tissues. In this process, formaldehyde will drip and the tissue will slip, and it will bring great health risks to the experimenters. Under these uncontrollable factors, we cannot estimate how much harm formaldehyde will cause to the body of the experimenters during the experiment. For a long time, the experimental operation of replacing the formaldehyde fixative has caused us great trouble and psychological pressure, and many students have reported that they will have obvious physical discomfort for several consecutive days after contacting formaldehyde.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an animal tissue fixing device for automatically replacing formaldehyde, comprising a sample preservation tube, wherein a fixing device for fixing animal tissue is arranged inside the sample preservation tube, a discharge device for discharging fresh formaldehyde is arranged on the upper side of the sample preservation tube, and an extraction device for discharging waste liquid formaldehyde is arranged on the lower side of the sample preservation tube;

[0006] The fixing device includes a flow guide cover plugged into the upper side of the inner wall of the sample preservation tube, a mesh plate fixedly connected to the middle of the inner side of the sample preservation tube, a plurality of animal tissue positioning cylinders plugged into the upper side of the mesh plate, and a flow guide cylinder fixedly connected to the lower side of the mesh plate;

[0007] The discharge device comprises a special cover for the sample preservation tube threadedly connected to the upper side of the sample preservation tube, a rubber hose channel for infusing new liquid plugged into the middle of the inner side of the special cover for the sample preservation tube, a fresh formaldehyde storage tank connected to the side of the rubber hose channel for infusing new liquid away from the special cover for the sample preservation tube, and a multi-channel air power pump connected to the side of the rubber hose channel for infusing new liquid away from the fresh formaldehyde storage tank;

[0008] The extraction device comprises a waste liquid suction rubber hose channel connected to the lower side of the sample preservation tube and a formaldehyde waste liquid storage tank connected to the side of the waste liquid suction rubber hose channel away from the sample preservation tube.

[0009] Furthermore, a connecting component for connecting the rubber hose channel for injecting new liquid is arranged on the upper side of the special cover for the sample preservation tube, and the connecting component includes a connecting pipe port inserted in the middle of the upper side of the special cover for the sample preservation tube.

[0010] Furthermore, a flow component for flowing formaldehyde gas in the rubber hose channel for infusing new liquid and the connecting pipe mouth is arranged on the outside of the multi-channel air dynamic pump, and the flow component includes a first multi-channel air dynamic pump fixedly installed on the upper left end of the multi-channel air dynamic pump and a second multi-channel air dynamic pump fixedly installed on the lower left end of the multi-channel air dynamic pump.

[0011] Furthermore, the front end face of the multi-channel air dynamic pump is provided with a driving assembly for cooperating with the multi-channel air dynamic pump to drive the rubber hose channel and the connecting pipe mouth for injecting new liquid, and the driving assembly includes a waste liquid suction switch fixedly installed on the upper side of the front end face of the multi-channel air dynamic pump and a new liquid injection switch fixedly installed on the lower side of the front end face of the multi-channel air dynamic pump.

[0012] Furthermore, a control component for controlling the multi-channel air dynamic pump is disposed on the upper side of the multi-channel air dynamic pump, and the control component includes a power switch fixedly mounted on the upper side of the multi-channel air dynamic pump.

[0013] Furthermore, the new liquid infusion rubber hose channel is penetrated by a plug-in connection pipe opening close to one side of the special cover of the sample preservation tube, and the outer wall on the upper side of the sample preservation tube is provided with an external thread.

[0014] Furthermore, an inner wall of the special cover for the sample preservation tube is provided with an internal thread, and the internal thread of the special cover for the sample preservation tube forms a threaded connection with the external thread of the sample preservation tube, the guide cover is a pointed cone structure and is inserted into the upper surface of the mesh plate, the guide tube is an inverted cone structure, and the inner side of the guide tube is hollow and connected to the mesh plate.

[0015] Furthermore, the plurality of animal tissue positioning cylinders are arranged equidistantly in the transverse direction, the lower side of the guide cylinder and one end of the waste liquid suction rubber hose channel are in an up-and-down structure, and the connecting pipe opening is a double-channel and double-filter structure.

[0016] Compared with the prior art, the utility model provides an animal tissue fixation device for automatically replacing formaldehyde, which has the following beneficial effects:

[0017] 1. The animal tissue fixing device for automatically replacing formaldehyde places the tissue in the animal tissue positioning cylinder on the upper side of the mesh plate in the sample preservation tube and immerses it in formaldehyde. When formaldehyde flows from the channel of the rubber hose for perfusing new liquid into the sample preservation tube, it will slowly move toward the side wall, pass through the guide cover and flow into the guide cylinder. After use, it will be drawn out from the hole below the sample preservation tube, avoiding the tissue from being directly washed away and contaminated and being damaged, effectively ensuring and improving the protection of the tissue in the sample preservation tube from contamination and damage, and the cleaning and fixing method is gentle.

[0018] 2. The animal tissue fixing device for automatically replacing formaldehyde allows formaldehyde to enter the sample preservation tube through the new liquid infusion rubber hose channel and then discharges the formaldehyde through the waste liquid suction rubber hose channel. The discharged formaldehyde is transported to the formaldehyde waste liquid storage tank, and fresh formaldehyde is continuously discharged into the sample preservation tube through a multi-channel air power pump combined with the new liquid infusion rubber hose channel, ensuring that many tanks for processing tissues can be connected in series and multiple tissues can be processed at the same time, greatly improving efficiency, saving labor and time, and solving the problem of using and replacing formaldehyde in animal tissue experiments. However, formaldehyde is very harmful to experimenters. When replacing formaldehyde for tissues, most laboratories use the primitive method of manually dumping formaldehyde waste liquid and injecting fresh formaldehyde with a syringe. During this process, formaldehyde may drip and tissue may slip, which poses a great health hazard to the experimenters. Due to these uncontrollable factors, we cannot estimate the extent of the harm that formaldehyde will cause to the bodies of the experimenters during the experiment. For a long time, the experimental operation of replacing the formaldehyde fixative has caused us great trouble and psychological pressure. At the same time, many students reported that they had obvious physical discomfort for several consecutive days after contact with formaldehyde. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the structure of the utility model;

[0020] Figure 2 This is a structural cross-sectional view of the sample storage tube of the utility model;

[0021] Figure 3 This is an exploded view of the structure of the sample storage tube of the utility model.

[0022] In the figure: 1 a special cover for sample preservation tube, 2 a sample preservation tube, 3 a formaldehyde waste liquid storage tank, 4 a fresh formaldehyde storage tank, 5 a multi-channel air dynamic pump, 6 a power switch, 7 a waste liquid suction switch, 8 a new liquid injection switch, 9 a new liquid injection rubber hose channel, 10 a waste liquid suction rubber hose channel, 11 a connecting pipe mouth, 12 a first multi-channel air dynamic pump, 13 a second multi-channel air dynamic pump, 14 a guide cover, 15 a mesh plate, 16 an animal tissue positioning tube, and 17 a guide tube. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] See also Figure 1-3 , an animal tissue fixing device for automatically replacing formaldehyde, comprising a sample preservation tube 2, a fixing device for fixing animal tissues is arranged inside the sample preservation tube 2, a discharge device for discharging fresh formaldehyde is arranged on the upper side of the sample preservation tube 2, and an extraction device for discharging waste liquid formaldehyde is arranged on the lower side of the sample preservation tube 2. The fixing device comprises a flow guide cover 14 plugged into the upper side of the inner wall of the sample preservation tube 2, a mesh plate 15 fixedly connected to the middle of the inner side of the sample preservation tube 2, a plurality of animal tissue positioning cylinders 16 plugged into the upper side of the mesh plate 15, and a flow guide cylinder 17 fixedly connected to the lower side of the mesh plate 15. The discharge device comprises a sample preservation tube special cover 1 threadedly connected to the upper side of the sample preservation tube 2, a new liquid perfusion rubber hose channel 9 plugged into the middle of the inner side of the sample preservation tube special cover 1, a fresh formaldehyde storage tank 4 connected to the new liquid perfusion rubber hose channel 9 away from the sample preservation tube special cover 1, and a multi-channel air power pump 5 connected to the new liquid perfusion rubber hose channel 9 away from the fresh formaldehyde storage tank 4. The extraction device comprises a waste liquid suction rubber hose channel 10 connected to the lower side of the sample preservation tube 2 and a formaldehyde waste liquid storage tank 3 connected to the side of the waste liquid suction rubber hose channel 10 away from the sample preservation tube 2 .

[0025] Among them, a connecting component for connecting the rubber hose channel 9 for perfusing new liquid is arranged on the upper side of the special cover 1 for the sample preservation tube, and the connecting component includes a connecting pipe mouth 11 inserted in the middle part of the upper side of the special cover 1 for the sample preservation tube, and a flow component for the flow of formaldehyde gas in the rubber hose channel 9 for perfusing new liquid and the connecting pipe mouth 11 is arranged on the outer side of the multi-channel air dynamic pump 5, and the flow component includes a first multi-channel air dynamic pump 12 fixedly installed on the upper left end of the multi-channel air dynamic pump 5 and a second multi-channel air dynamic pump 13 fixedly installed on the lower left end of the multi-channel air dynamic pump 5, and a driving component for driving the rubber hose channel 9 for perfusing new liquid and the connecting pipe mouth 11 in coordination with the multi-channel air dynamic pump 5 is arranged on the front end surface of the multi-channel air dynamic pump 5, and the driving component includes a suction waste liquid switch 7 fixedly installed on the upper side of the front end surface of the multi-channel air dynamic pump 5 and a second multi-channel air dynamic pump 13 fixedly installed on the front end of the multi-channel air dynamic pump 5. A new liquid injection switch 8 is provided on the lower side of the surface, and a control component for controlling the multi-channel air dynamic pump 5 is provided on the upper side of the multi-channel air dynamic pump 5, the control component includes a power switch 6 fixedly installed on the upper side of the multi-channel air dynamic pump 5, and the new liquid injection rubber hose channel 9 is connected to the connecting pipe mouth 11 near the side of the special cover 1 for the sample preservation tube, and the outer wall of the upper side of the sample preservation tube 2 is provided with an external thread, and the inner wall of the special cover 1 for the sample preservation tube is provided with an internal thread, and the internal thread of the special cover 1 for the sample preservation tube forms a threaded connection with the external thread of the sample preservation tube 2, the guide cover 14 is a pointed cone structure and is inserted into the upper surface of the mesh plate 15, the guide tube 17 is an inverted cone structure, and the inner side of the guide tube 17 is hollowed out and connected with the mesh plate 15, a plurality of animal tissue positioning tubes 16 are arranged equidistantly in the horizontal direction, and the lower side of the guide tube 17 and one end of the rubber hose channel 10 for sucking waste liquid are in an up-and-down structure, and the connecting pipe mouth 11 is a double-channel and double-filter structure.

[0026] It should be noted that, during the whole process, there is no need to frequently change formaldehyde manually. It is only necessary to set the formaldehyde replacement time on the multi-channel pneumatic pump 5. The experimenters will no longer be harmed by contact with formaldehyde. Multiple tanks can be connected in series for absorption, and multiple tissues can be processed at the same time, which greatly improves the experimental efficiency and saves labor and time. The animal tissue positioning cylinder 16 can be used to regulate the tissues to avoid the experimenters manually taking the tissues away, thus avoiding the tissues from being damaged and contaminated during the processing, and ensuring that the experimental materials are free from infection. The design of the sample preservation tube 2 also protects the tissues from contamination and damage, and the guide cover 14 ensures the gentleness of the formaldehyde flow, reducing the damage caused by pollution of the formaldehyde direct flushing machine.

[0027] It should be noted that the experimenter can set the number of animal tissue positioning cylinders 16 to 16, 24 or 32 according to the needs and place them on the upper side of the mesh plate 15.

[0028] The working principle of the above embodiment is:

[0029] The dedicated cover 1 for the sample preservation tube is separated from the upper thread of the sample preservation tube 2, and the guide cover 14 is taken out of the sample preservation tube 2 to expose the animal tissue positioning tube 16 on the upper side of the mesh plate 15, and the excised animal tissues are sequentially placed in the animal tissue positioning tube 16 to be fixed, and the guide cover 14 is clamped on the upper side end of the mesh plate 15 again, the dedicated cover 1 for the sample preservation tube is tightened with the sample preservation tube 2 and the rubber hose channel 9 for infusing new liquid is inserted and fixed from the connecting pipe port 11 on the upper side of the dedicated cover 1 for the sample preservation tube, and the rubber hose channel 10 for sucking waste liquid is inserted and fixed from the hole on the lower side of the sample preservation tube 2, and the rubber hose channel 10 for sucking waste liquid is also connected to the formaldehyde waste liquid storage tank 3 and the multi-channel air power pump 5 respectively, and the rubber hose channel 9 for infusing new liquid is connected to the fresh formaldehyde storage tank 4 The installation of the whole device is completed by connecting it with the multi-channel air dynamic pump 5, and then the power switch 6 is started to turn on the multi-channel air dynamic pump 5. The new liquid injection switch 8 is pressed to absorb the formaldehyde in the fresh formaldehyde storage tank 4 through the new liquid injection rubber hose channel 9 and as the first multi-channel air dynamic pump 12 is started, it is discharged from the connecting pipe port 11 to the sample preservation tube 2. The formaldehyde slowly flows downward from the upper side wall of the sample preservation tube 2 and passes through the guide cover 14 to contact the tissue in the animal tissue positioning cylinder 16, and then passes through the mesh plate 15 and flows from the guide cylinder 17 to the bottom of the sample preservation tube 2. Then, the waste liquid suction switch 7 is turned on to absorb the formaldehyde waste liquid through the second multi-channel air dynamic pump 13 and the waste liquid suction rubber hose channel 10 and transfer it to the formaldehyde waste liquid storage tank 3 to complete the whole process.

[0030] The electrical components appearing in the article are all electrically connected to the main controller and the power supply. The main controller can be a conventional known device for control such as a computer, and the existing public power connection technology is not described in detail in the article.

[0031] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0032] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An animal tissue fixation device for automatically replacing formaldehyde, comprising a sample preservation tube (2), characterized in that: The sample preservation tube (2) is provided with a fixing device for fixing animal tissue inside, the sample preservation tube (2) is provided with a discharge device for discharging fresh formaldehyde on the upper side, and the sample preservation tube (2) is provided with an extraction device for discharging waste liquid formaldehyde on the lower side; The fixing device comprises a flow guide cover (14) plugged into the upper side of the inner wall of the sample preservation tube (2), a mesh plate (15) fixedly connected to the middle part of the inner side of the sample preservation tube (2), a plurality of animal tissue positioning cylinders (16) plugged into the upper side of the mesh plate (15), and a flow guide cylinder (17) fixedly connected to the lower side of the mesh plate (15); The discharge device comprises a special cover (1) for the sample preservation tube threadedly connected to the upper side of the sample preservation tube (2), a new liquid perfusion rubber hose channel (9) plugged into the middle of the inner side of the special cover (1) for the sample preservation tube, a fresh formaldehyde storage tank (4) connected to the side of the new liquid perfusion rubber hose channel (9) away from the special cover (1) for the sample preservation tube, and a multi-channel air power pump (5) connected to the side of the new liquid perfusion rubber hose channel (9) away from the fresh formaldehyde storage tank (4); The extraction device comprises a waste liquid suction rubber hose channel (10) plugged and connected to the lower side of the sample preservation tube (2) and a formaldehyde waste liquid storage tank (3) connected to the waste liquid suction rubber hose channel (10) at a side away from the sample preservation tube (2).

2. The animal tissue fixation device for automatically replacing formaldehyde according to claim 1, characterized in that: The upper side of the dedicated cover (1) for the sample storage tube is provided with a connecting component for connecting the rubber hose channel (9) for injecting new liquid. The connecting component comprises a connecting pipe opening (11) plugged into the middle part of the upper side of the dedicated cover (1) for the sample storage tube.

3. The animal tissue fixation device for automatically replacing formaldehyde according to claim 1, characterized in that: A flow assembly for controlling the flow of formaldehyde gas in the rubber hose channel (9) for infusing new liquid and the connecting pipe port (11) is arranged on the outside of the multi-channel air dynamic pump (5), and the flow assembly comprises a first multi-channel air dynamic pump (12) fixedly mounted on the upper left end of the multi-channel air dynamic pump (5) and a second multi-channel air dynamic pump (13) fixedly mounted on the lower left end of the multi-channel air dynamic pump (5).

4. The animal tissue fixation device for automatically replacing formaldehyde according to claim 1, characterized in that: The front end surface of the multi-channel air dynamic pump (5) is provided with a driving assembly for driving the rubber hose channel (9) for injecting new liquid and the connecting pipe port (11) in cooperation with the multi-channel air dynamic pump (5), and the driving assembly comprises a waste liquid suction switch (7) fixedly mounted on the upper side of the front end surface of the multi-channel air dynamic pump (5) and a new liquid injection switch (8) fixedly mounted on the lower side of the front end surface of the multi-channel air dynamic pump (5).

5. The animal tissue fixation device for automatically replacing formaldehyde according to claim 1, characterized in that: A control component for controlling the multi-channel air dynamic pump (5) is arranged on the upper side of the multi-channel air dynamic pump (5), and the control component comprises a power switch (6) fixedly mounted on the upper side of the multi-channel air dynamic pump (5).

6. The animal tissue fixation device for automatically replacing formaldehyde according to claim 1, characterized in that: The new liquid infusion rubber hose channel (9) is inserted into and connected to a pipe opening (11) on one side of the dedicated cover (1) for the sample storage tube, and an external thread is provided on the outer wall of the upper side of the sample storage tube (2).

7. The animal tissue fixation device for automatically replacing formaldehyde according to claim 6, characterized in that: The inner wall of the dedicated cover (1) for the sample storage tube is provided with an internal thread, and the internal thread of the dedicated cover (1) for the sample storage tube is threadedly connected with the external thread of the sample storage tube (2); the flow guide cover (14) is in a pointed cone structure and is inserted into the upper surface of the mesh plate (15); the flow guide tube (17) is in an inverted cone structure, and the inner side of the flow guide tube (17) is hollowed out and communicated with the mesh plate (15).

8. The animal tissue fixation device for automatically replacing formaldehyde according to claim 2, characterized in that: The plurality of animal tissue positioning cylinders (16) are arranged in a horizontally equidistant manner, the lower side of the guide cylinder (17) and one end of the waste liquid suction rubber hose channel (10) are in an up-and-down structure, and the connecting pipe opening (11) is a double-channel and double-filter structure.

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