Reaction device for preparing colloidal gold

Abstract

The utility model relates to a reaction device for preparing colloidal gold, which comprises a sealed reaction cavity communicated with a condensing device. The stirring device comprises a stirring driving device, a stirring rod and a stirrer; the stirring driving device is arranged outside the reaction cavity; the stirring rod is connected with the stirring driving device and extends into the reaction cavity; the stirrer is arranged at the free end of the stirring rod; the center of the stirring rod and the center of the stirrer are hollow bodies which are communicated with each other, and pores are formed in the surface of the stirrer; the reaction kettle further comprises a feeding device, the feeding device is arranged outside the reaction cavity, an outlet of the feeding device is connected with a feeding pipe, and the free end of the feeding pipe extends into the hollow body in the stirring rod, so that materials are added into the stirrer through the stirring rod, and the stirring rod is connected with the stirring rod. And further, the gas enters the reaction cavity through pores in the surface of the stirrer. When the reaction device is used for preparing colloidal gold, the product difference among different batches of production can be reduced.

Description

Technical Field

[0001] The utility model relates to a preparation technology of colloidal gold, in particular to a reaction device for preparing colloidal gold. Background Art

[0002] Colloidal gold is a colloidal solution with stable dispersion properties. Its main component is nano-gold particles with negatively charged surfaces. These gold particles have strong adsorption to proteins under certain conditions and can bind firmly to proteins. This combination, whether in liquid or solid state, can maintain the biological activity of proteins for a long time and stably. Therefore, colloidal gold is often used as a color indicator for immunoassay reagents.

[0003] The formation of colloidal gold particles mainly goes through two stages: "nucleation and continuous growth". Taking the sodium citrate reduction method to prepare colloidal gold as an example, it includes the following stages:

[0004] Nucleation stage: chloroauric acid (HAUCl4) is quickly reduced to gold atoms under the reduction effect of sodium citrate, and its concentration quickly increases until it is supersaturated, and crystal nuclei are formed as agglomeration occurs;

[0005] Growth stage: Once the crystal nucleus is formed, other gold atoms in the solution will continue to bind to the gold nucleus to form colloidal gold particles with stable particle size and shape.

[0006] In the above process, the formation of colloidal gold particles with uniform particle size and stable shape is the key factor in maintaining the stable performance of the cross-linked product (protein-colloidal gold conjugate). Irregular shape or uneven particle size will lead to the instability of the cross-linked product, which is easy to dissociate and precipitate during the application process, which is reflected in the chromatography test strip as incomplete chromatography, showing too high background or false positive results.

[0007] The specific method for preparing colloidal gold in the traditional way is the citrate reduction method, and the specific operation is as follows:

[0008] (1) Prepare HAUCl4 into a 0.01% aqueous solution and heat 100 ml to boiling.

[0009] (2) Add a certain amount of 1% trisodium citrate (Na3C6H5O7·2H2O) aqueous solution while stirring.

[0010] (3) Continue heating and boiling for 15 minutes. At this time, it can be observed that the light yellow chloroauric acid aqueous solution quickly turns gray after the addition of sodium citrate, then turns black, and then gradually stabilizes to red. The whole process takes about 2 to 3 minutes.

[0011] (4) After cooling to room temperature, restore the volume to its original volume with distilled water.

[0012] There are many areas that are difficult to control when using the above preparation method in actual production, including:

[0013] (1) During the preparation, a round-bottom flask was used with aluminum foil covering the flask mouth. When boiling, the amount of water volatilized could not be controlled, and the actual concentration of HAUCl4 solution was not controlled, resulting in batch differences;

[0014] (2) When adding trisodium citrate, pour it from the bottle mouth. If the operation is not done properly, it is easy for it to stick to the bottle mouth. The actual amount of trisodium citrate added is different from the theoretical amount, resulting in batch differences.

[0015] (3) Different operators add trisodium citrate at different speeds, resulting in different solution reaction rates and uneven colloidal gold particle sizes, leading to batch differences.

[0016] Therefore, the current production of colloidal gold particles still has many deficiencies and urgently needs to be improved to obtain colloidal gold particles with uniform particle size and stable morphology, and the differences between different batches are as small as possible. Utility Model Content

[0017] In view of the above problems, the utility model provides a reaction device for preparing colloidal gold. By improving the reaction cavity, the stirring device and the feeding device, the process of preparing colloidal gold is easier to control, so that it is easy to obtain a product with uniform properties and reduce the difference between different batches of products.

[0018] In order to solve this technical problem, the utility model adopts the following solutions:

[0019] A reaction device for preparing colloidal gold, comprising a sealed reaction cavity, wherein the reaction cavity is connected to a condensation device;

[0020] The invention also comprises a stirring device, wherein the stirring device comprises a stirring driving device arranged outside the reaction cavity, a stirring rod connected to the stirring driving device and extending into the reaction cavity, and a stirring bar arranged at the free end of the stirring rod; wherein the centers of the stirring rod and the stirring bar are connected to each other as a hollow body, and the surface of the stirring bar has pores;

[0021] It also includes a feeding device, which is arranged outside the reaction cavity. The outlet of the feeding device is connected to a feeding pipe, and the free end of the feeding pipe extends into the hollow body of the stirring rod, so that the material is added into the stirring bar through the stirring rod, and then enters the reaction cavity through the pores on the surface of the stirring bar.

[0022] Furthermore, the reaction cavity is a two-necked flask or a three-necked flask.

[0023] Furthermore, the reaction cavity is a round-bottom flask.

[0024] Furthermore, the condensing device is a reflux pipe.

[0025] Furthermore, the feeding device is a syringe, the feeding tube is a rubber tube, and the diameter of the rubber tube is adapted to the outlet of the syringe.

[0026] The feeding device comprises a sealed material tank and a peristaltic pump connected to the material tank, and the peristaltic pump is connected to the feeding pipe.

[0027] Furthermore, the pore size of the stirring rotor is 0.1-10 mm.

[0028] Furthermore, the pore size of the stirring rotor is 0.5-5 mm.

[0029] By adopting the above technical solution, the utility model has the following advantages compared with the prior art:

[0030] (1) Compared with the conventional flask which is not sealed, water vapor will evaporate during the heating process, resulting in changes in the concentration of the chloroauric acid solution. In the device adopted by the utility model, the reaction cavity is sealed, which reduces the evaporation of water vapor, ensures the consistency of the concentration during the preparation process, and is conducive to forming a more stable reaction environment.

[0031] (2) A feeding device is used to control the speed at which the reaction materials enter the reaction cavity, and the small holes on the stirring bar are used to allow the reducing agent to be completely dispersed into the chloroauric acid solution, making the reaction more complete and the reaction speed controllable, thereby obtaining colloidal gold of uniform quality, greatly reducing the differences in products between different reaction batches.

[0032] (3) The entire process of using the device can be automated, and the addition of the reducing agent can also be completed by an electric device, which reduces the deviation caused by manual operation and improves accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a schematic diagram of the structure of the device provided in Example 1 of the utility model;

[0034] Figure 2 It is a schematic diagram of the device structure provided in Example 2 of the utility model. DETAILED DESCRIPTION

[0035] The technical solution of the utility model will be clearly and completely described below in conjunction with the accompanying drawings and specific embodiments, but those skilled in the art will understand that the embodiments described below are part of the embodiments of the utility model, rather than all of the embodiments, and are only used to illustrate the utility model, and should not be considered as limiting the scope of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the utility model. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0036] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0037] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0038] Embodiment 1:

[0039] refer to Figure 1 , a reaction device for preparing colloidal gold, comprising a sealed reaction cavity 1, wherein the reaction cavity 1 is connected to a condensation device 2;

[0040] The stirring device further comprises a stirring device, wherein the stirring device comprises a stirring driving device 3 arranged outside the reaction cavity 1, a stirring rod 4 connected to the stirring driving device 3 and extending into the reaction cavity 1, and a stirring bar 5 arranged at the free end of the stirring rod 4; wherein the centers of the stirring rod 4 and the stirring bar 5 are connected to each other as a hollow body, and the surface of the stirring bar 5 has pores;

[0041] It also includes a feeding device 6, which is arranged outside the reaction cavity 1, and the outlet of the feeding device 6 is connected to a feeding pipe 7, and the free end of the feeding pipe 7 extends into the hollow body of the stirring rod 4, so that the material is added to the stirring bar 5 through the stirring rod 4, and then enters the reaction cavity 1 through the pores on the surface of the stirring bar 5. The appropriate pore size is 0.5-5mm. When the volume of the reaction cavity 1 is large, for example, more than 5L, a pore of 10mm can also be used; when the volume of the reaction cavity 1 is small, for example, less than 1L, a pore of 0.1mm can also be used.

[0042] In this embodiment, the reaction cavity 1 adopts a three-necked flask, preferably a round-bottomed flask, so that the reaction materials are mixed more evenly. In other embodiments, a two-necked flask can also be used.

[0043] In this embodiment, the middle opening of the three-necked flask is used to connect the stirring rod 4, and the two symmetrical side openings are respectively connected to the condensing device 2. The condensing device 2 can adopt a reflux tube with water as the outer condensing medium.

[0044] In this embodiment, the feeding device 6 is a syringe, and the material enters the reaction cavity 1 by manual or electric control, specifically by pushing the syringe, so that the liquid material enters the stirring bar 5 through the feeding tube 7. When the stirring driving device 3 is turned on, the stirring rod 4 is driven to rotate, and the stirring bar 5 is then driven to rotate, and the material in the stirring bar 5 is thrown out and enters the reaction cavity 1, and is mixed with another material previously added to the reaction cavity 1, and then a chemical reaction occurs.

[0045] The method for preparing colloidal gold using the above device is as follows:

[0046] (1) Add a chloroauric acid solution of appropriate concentration into the reaction cavity 1, place the reaction cavity 1 on an electric heating mantle (or other heating device), connect the stirring drive device 3 and the stirring rod 4 to the reaction cavity 1, and confirm that the seal is intact;

[0047] (2) Heat and stir at a constant speed until the chloroauric acid solution boils, and continue heating for 2 to 3 minutes;

[0048] (3) The selected reducing agent is sealed in the feeding device 6, and the selected reducing agent is injected through the feeding pipe 7 to carry out a chemical reaction.

[0049] The preparation device is used to prepare colloidal gold, and the batch difference of colloidal gold preparation can be effectively controlled; by preparing three batches of colloidal gold solutions, verification was carried out on an influenza A / influenza B detection kit (colloidal gold method), and the results showed that the batch differences of the three batches of colloidal gold solutions prepared were obvious due to the traditional method.

[0050] Embodiment 2:

[0051] refer to Figure 2 , a reaction device for preparing colloidal gold, comprising a sealed reaction cavity 1, wherein the reaction cavity 1 is connected to a condensation device 2;

[0052] The stirring device further comprises a stirring device, wherein the stirring device comprises a stirring driving device 3 arranged outside the reaction cavity 1, a stirring rod 4 connected to the stirring driving device 3 and extending into the reaction cavity 1, and a stirring bar 5 arranged at the free end of the stirring rod 4; wherein the centers of the stirring rod 4 and the stirring bar 5 are connected to each other as a hollow body, and the surface of the stirring bar 5 has pores;

[0053] It also includes a feeding device, which includes a sealed material tank 8 and a peristaltic pump 61 connected to the material tank 8. The material tank 8 and the peristaltic pump 61 are arranged outside the reaction cavity 1. The material tank 8 is used to store the reaction material, and the reaction material is transported to the feeding pipe 7 through the peristaltic pump 61.

[0054] The free end of the feeding tube 7 extends into the hollow body of the stirring rod 4 , so that the reaction materials are added into the stirring bar 5 , and then enter the reaction cavity 1 through the pores on the surface of the stirring bar 5 .

[0055] The method for preparing colloidal gold using the above device is as follows:

[0056] (1) Add a chloroauric acid solution of appropriate concentration into the reaction cavity 1, place the reaction cavity 1 on an electric heating mantle (or other heating device), connect the stirring drive device 3 and the stirring rod 4 to the reaction cavity 1, and confirm that the seal is intact;

[0057] (2) Heat and stir at a constant speed until the chloroauric acid solution boils, and continue heating for 2 to 3 minutes;

[0058] (3) The selected reducing agent is sealed in the material tank 8, and the selected reducing agent is injected through the feeding pipe 7 by the peristaltic pump 61 to carry out a chemical reaction.

[0059] The preparation device is used to prepare colloidal gold, and the batch difference of colloidal gold preparation can be effectively controlled; by preparing three batches of colloidal gold solutions, verification was carried out on an influenza A / influenza B detection kit (colloidal gold method), and the results showed that the batch differences of the three batches of colloidal gold solutions prepared were obvious due to the traditional method.

[0060] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are illustrative and cannot be understood as limitations on the present invention. Ordinary technicians in the field can change, modify, replace and modify the above embodiments within the scope of the present invention without departing from the principles and purpose of the present invention.

Claims

1. A reaction device for preparing colloidal gold, characterized in that: It includes a sealed reaction cavity, wherein the reaction cavity is connected to a condensation device; The invention also comprises a stirring device, wherein the stirring device comprises a stirring driving device arranged outside the reaction cavity, a stirring rod connected to the stirring driving device and extending into the reaction cavity, and a stirring bar arranged at the free end of the stirring rod; wherein the centers of the stirring rod and the stirring bar are connected to each other as a hollow body, and the surface of the stirring bar has pores; It also includes a feeding device, which is arranged outside the reaction cavity. The outlet of the feeding device is connected to a feeding pipe, and the free end of the feeding pipe extends into the hollow body of the stirring rod, so that the material is added into the stirring bar through the stirring rod, and then enters the reaction cavity through the pores on the surface of the stirring bar.

2. The reaction device for preparing colloidal gold according to claim 1, characterized in that: The reaction cavity is a two-necked flask or a three-necked flask.

3. The reaction device for preparing colloidal gold according to claim 2, characterized in that: The reaction cavity is a round-bottom flask.

4. The reaction device for preparing colloidal gold according to claim 1, characterized in that: The condensing device is a reflux pipe.

5. The reaction device for preparing colloidal gold according to any one of claims 1 to 4, characterized in that: The feeding device is a syringe, and the feeding tube is a rubber tube, the diameter of which is matched with the outlet of the syringe.

6. The reaction device for preparing colloidal gold according to any one of claims 1 to 4, characterized in that: The feeding device comprises a sealed material tank and a peristaltic pump connected to the material tank, and the peristaltic pump is connected to the feeding pipe.

7. The reaction device for preparing colloidal gold according to any one of claims 1 to 4, characterized in that: The pore size of the stirring bar is 0.1-10 mm.

8. The reaction device for preparing colloidal gold according to claim 7, characterized in that: The pore size of the stirring bar is 0.5-5 mm.

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