48-hole double-color PCR (Polymerase Chain Reaction) plate

Abstract

The utility model discloses a 48-hole two-color PCR (Polymerase Chain Reaction) plate which comprises a one-color 48-hole body and a plurality of two-color tube bodies which are uniformly distributed on the one-color 48-hole body. The one-color 48-hole body made of the PC material and the two-color tube body made of the PP material are injection-molded into a whole, so that the product is small in deformation, good in flatness and more stable in film sealing after being molded, good stability can be provided in the pipetting process, and treatment of an automatic robot is facilitated; compared with a 96-hole PCR plate, the PCR plate is more flexible in use and can be used for detecting a small number of samples, the dosage of a detection reagent is reduced, and the detection cost is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of PCR plates, and particularly relates to a 48-well double-color PCR plate. Background Art

[0002] At present, PCR plates on the market are generally made of PP materials, usually single-color 96-well PCR plates, which are widely used in genetics, biochemistry, immunology, medicine and other fields. They are not only used in basic research such as gene separation, cloning and nucleic acid sequence analysis, but also can be used for disease diagnosis or any place with DNA and RNA; but the material has low hardness, and after being exposed to high temperature of 121°C and low temperature of -80°C, the product shrinks greatly, is prone to deformation, and has poor surface flatness, which leads to the risk of leakage of the sealing film and unstable performance. Utility Model Content

[0003] Purpose of the utility model: In order to overcome the above shortcomings, the purpose of the utility model is to provide a 48-well two-color PCR plate, which has the advantages of small deformation, good flatness, more stable sealing film, and better stability during pipetting.

[0004] Technical solution: In order to achieve the above purpose, the utility model provides a 48-well two-color PCR plate, including:

[0005] A single-color 48-well body and a plurality of two-color tubes evenly distributed on the single-color 48-well body.

[0006] Furthermore, the one-color 48-hole body includes a base, a side panel, and a top panel; the side panel is connected to the base in a stepped manner and narrows from bottom to top in a trapezoidal shape, a top panel is provided above the side panel, and a plurality of perforations are evenly provided on the top panel; the setting of the base increases its own stability.

[0007] Furthermore, a group of reinforcing ribs are provided around the inner wall of the side panel, which improves the overall connection strength of the 48-hole body, thereby improving its own good structural firmness.

[0008] Furthermore, the top plate is provided with transverse serial numbers at the edge along the length direction, and with longitudinal serial numbers at the edge along the width direction; the markings designed in the length direction and the width direction can facilitate the inspectors to read the inspection results quickly and accurately.

[0009] The upper end of the two-color tube body is cylindrical, and the lower end is conical. The outer side of the upper end is provided with a limit snap-on connected to the perforation on the top plate; it can be better connected to the one-color 48-hole body into one during injection molding.

[0010] The tube mouth of the two-color tube body is higher than the upper surface of the top plate, which is convenient for sealing the sealing film, increases the sealing performance, and prevents problems such as liquid leakage, volatilization, and cross contamination.

[0011] The one-color 48-hole body is made of PC material; the thermal expansion rate of PC material is much smaller than that of PP material. The one-color 48-hole body made of PC material is more thermally stable, which reduces the thermal expansion rate and shrinkage rate and reduces deformation.

[0012] The two-color tube body is made of PP.

[0013] It can be seen from the above technical solution that the utility model has the following beneficial effects:

[0014] 1. The utility model provides a 48-well two-color PCR plate, which is formed into a whole by injection molding of a one-color 48-well body made of PC material and a two-color tube body made of PP material. After the product is formed, the deformation is small, the flatness is good, the sealing film is more stable, and better stability can be provided during the pipetting process.

[0015] 2. The utility model provides a 48-well two-color PCR plate, which can reduce the number of samples and the amount of detection reagents used, thereby reducing the detection cost, compared with a single-color 96-well PCR plate.

[0016] 3. The utility model provides a 48-well two-color PCR plate, and the corresponding detection equipment is light in weight, small in size, and easy to carry. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a cross-sectional view of a 48-well dual-color PCR plate described in the utility model;

[0018] Figure 2 This is a schematic diagram of the structure of a 48-hole body of one color described in the utility model;

[0019] Figure 3 It is a structural schematic diagram of the two-color tube body described in the utility model;

[0020] Figure 4 This is a schematic diagram of the structure of a 48-well dual-color PCR plate described in the utility model;

[0021] In the figure: 1 one-color 48-hole body, 11 base, 12 side plate, 13 through hole, 14 reinforcing rib, 15 top plate, 2 two-color tube body. DETAILED DESCRIPTION

[0022] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0023] Example 1

[0024] A 48-well two-color PCR plate, such as Figures 1 to 4 As shown, including:

[0025] like Figure 1 As shown, a single-color 48-hole body 1 and a plurality of two-color tube bodies 2 evenly distributed on the single-color 48-hole body 1.

[0026] like Figure 2 As shown, the 48-hole body 1 includes a base 11, a side panel 12 and a top panel 15; the side panel 12 is connected to the base 11 in a stepped manner and narrows from bottom to top in a trapezoidal shape, and a top panel 15 is provided above the side panel 12, and a plurality of through holes 13 are evenly provided on the top panel 15; in this embodiment, 48 through holes 13 are provided on the top panel 15.

[0027] The upper end of the two-color tube body 2 is cylindrical, and the lower end is conical. A limit stopper connected to the through hole 13 is provided on the outer side of the upper end portion.

[0028] In this embodiment, the number of the two-color tube bodies 2 is 48, arranged in eight rows and six columns, and connected with the through holes 13 on the top plate 15 .

[0029] The tube opening of the two-color tube body 2 is higher than the upper surface of the top plate 15 .

[0030] In this embodiment, the main body 1 with 48 holes of one color is made of PC material.

[0031] In this embodiment, the two-color tube body 2 is made of PP material.

[0032] Example 2

[0033] A 48-well two-color PCR plate, such as Figures 1 to 4 As shown, including:

[0034] like Figure 1 As shown, a single-color 48-hole body 1 and a plurality of two-color tube bodies 2 evenly distributed on the single-color 48-hole body 1.

[0035] like Figure 2 As shown, the 48-hole body 1 includes a base 11, a side panel 12 and a top panel 15; the side panel 12 is connected to the base 11 in a stepped manner and narrows from bottom to top in a trapezoidal shape, and a top panel 15 is provided above the side panel 12, and a plurality of through holes 13 are evenly provided on the top panel 15; in this embodiment, 48 through holes 13 are provided on the top panel 15.

[0036] A group of reinforcing ribs 14 are disposed around the inner wall of the side plate 12 .

[0037] The upper surface of the top plate 15 is provided with transverse serial numbers on the edge positions along the length direction. In this embodiment, the transverse serial numbers are A, B, C, D, E, F, G and H respectively; the edge positions along the width direction are provided with longitudinal serial numbers. In this embodiment, the longitudinal serial numbers are 1, 2, 3, 4, 5 and 6 respectively; corresponding identifications can be set according to actual needs to facilitate reading and recording.

[0038] The upper end of the two-color tube body 2 is cylindrical, and the lower end is conical, and a limit stopper connected to the through hole 13 is provided on the outer side of the upper end portion;

[0039] In this embodiment, the number of the two-color tube bodies 2 is 48, arranged in eight rows and six columns, and connected with the through holes 13 on the top plate 15 .

[0040] The tube opening of the two-color tube body 2 is higher than the upper surface of the top plate 15 .

[0041] In this embodiment, the main body 1 with 48 holes of one color is made of PC material.

[0042] In this embodiment, the two-color tube body 2 is made of PP material.

[0043] The production process of a 48-well two-color PCR plate described in this embodiment is as follows:

[0044] 1): Inject a single-color PC material into the mold of the 48-hole body, and wait for it to be formed into a single-color 48-hole body 1;

[0045] 2): Place the one-color 48-hole body 1 into the tube body mold, inject the two-color PP material into the mold, and wait for it to be formed, so that the one-color 48-hole body 1 and the two-color tube body 2 become a whole.

[0046] The above is only a preferred embodiment of the present invention. It should be pointed out that a person skilled in the art can make several improvements without departing from the principle of the present invention, and these improvements should also be regarded as within the protection scope of the present invention.

Claims

1. A 48-well two-color PCR plate, characterized in that: include: A single-color 48-hole body (1) and a plurality of two-color tube bodies (2) evenly distributed on the single-color 48-hole body (1); the single-color 48-hole body (1) comprises a base (11), a side plate (12) and a top plate (15); the side plate (12) is connected to the base (11) in a stepped manner and narrows from bottom to top in a trapezoidal shape; a top plate (15) is provided above the side plate (12), and a plurality of perforations (13) are evenly provided on the top plate (15); a group of reinforcing ribs (14) are provided around the inner wall of the side plate (12); the single-color 48-hole body (1) is made of PC material; The two-color tube body (2) is made of PP material.

2. A 48-well two-color PCR plate according to claim 1, characterized in that: The top plate (15) is provided with transverse serial numbers at edge positions along the length direction, and longitudinal serial numbers at edge positions along the width direction.

3. A 48-well two-color PCR plate according to claim 1, characterized in that: The upper end of the two-color tube body (2) is cylindrical, and the lower end is conical. A limit stopper connected to a through hole (13) on the top plate (15) is provided on the outer side of the upper end portion.

4. A 48-well two-color PCR plate according to claim 1, characterized in that: The tube opening of the two-color tube body (2) is higher than the upper surface of the top plate (15).

Images