Enrichment device for anaerobic ammonium oxidation bacteria

Abstract

The utility model discloses an enrichment device for anaerobic ammonium oxidation bacteria. The device comprises an enrichment unit and a control unit, the enrichment unit comprises one or more culture tanks and carriers fixed in the culture tanks; a liquid inlet and a liquid outlet are respectively formed in a tank body of the culture tank; the one or more culture tanks are connected in sequence; the culture tank is filled with a nutrient medium; the nutrient medium circularly flows in one or more culture tanks to form an aerobic and anaerobic environment. The enrichment device for the anaerobic ammonium oxidation bacteria is independently arranged outside a sewage treatment system, efficient enrichment is achieved, and the problem that the growth and reproduction of the anaerobic ammonium oxidation bacteria are slow due to the fact that the anaerobic ammonium oxidation bacteria are affected by pollutants in the sewage treatment system in the redebugging period is solved.

Description

Technical Field

[0001] The present application relates to the field of environmental engineering microbial technology, and in particular to an enrichment device for anaerobic ammonia-oxidizing bacteria. Background Art

[0002] In recent years, due to the increasing standards for urban sewage treatment in my country, the research and development of new biological denitrification processes is imminent. Anaerobic ammonium oxidation (anammox) is a wastewater denitrification process mediated by a type of Planctomycete bacteria and is a new type of biological denitrification technology.

[0003] Anaerobic ammonium oxidation (ANAOM) is a process in which anaerobic ammonium oxidizing bacteria use NO 2- As an electron acceptor, it oxidizes ammonia in wastewater into nitrogen gas, thus achieving the denitrification process. Compared with the traditional biological denitrification process, the anaerobic ammonium oxidation process does not require an external organic carbon source, which not only reduces the cost of organic carbon sources, but also reduces CO2 emissions during sewage treatment, promoting the realization of carbon neutrality.

[0004] However, the slow growth cycle and long doubling time of anaerobic ammonium oxidizing bacteria result in low sludge production, which in turn leads to a long startup cycle of anaerobic ammonium oxidizing reactors, which often takes hundreds of days, limiting their engineering applications. Therefore, how to quickly enrich anaerobic ammonium oxidizing bacteria is a problem that needs to be solved urgently. Summary of the invention

[0005] In order to solve the above-mentioned deficiencies in the art, the present application aims to provide an enrichment device for anaerobic ammonia-oxidizing bacteria. The enrichment method using the enrichment device of the present application can realize continuous enrichment culture and achieve rapid doubling.

[0006] According to one aspect of the present application, there is provided an anaerobic ammonia-oxidizing bacteria enrichment device comprising a pretreatment unit, a circulation unit, and an enrichment unit connected in sequence;

[0007] Wherein, the enrichment unit comprises: one or more culture tanks, and a carrier fixed in each tank body of the one or more culture tanks;

[0008] Each of the one or more culture tanks is provided with a liquid inlet and a liquid outlet on its body, and the liquid inlet is provided with a nozzle;

[0009] One or more culture tanks are connected in sequence;

[0010] Each of the one or more culture tanks is filled with a nutrient medium;

[0011] The nutrient substrate is circulated in one or more culture tanks to form an aerobic or anaerobic environment.

[0012] According to some embodiments of the present application, the carrier is a polyvinylidene chloride membrane;

[0013] According to some embodiments of the present application, there are 2 or more polyvinylidene chloride membranes;

[0014] According to some embodiments of the present application, the polyvinylidene chloride membrane has a mesh structure.

[0015] According to some embodiments of the present application, the circulation unit includes a circulation pump for circulating the flowing nutrient substrate, an efflux pump for effluxing the nutrient substrate, and a pipeline mixer.

[0016] According to some embodiments of the present application, the pretreatment unit includes: a nutrient dissolution tank, a nutrient matrix storage tank, a nutrient transfer pump, and a nutrient metering pump;

[0017] Wherein, the nutrient dissolution tank is connected to the nutrient matrix storage tank through a nutrient transfer pump;

[0018] The nutrient substrate storage tank is connected to the pipeline mixer through a nutrient metering pump.

[0019] The present application also provides a method for enriching anaerobic ammonia-oxidizing bacteria of the above-mentioned enrichment device, comprising:

[0020] fixing the carrier in a culture tank and inoculating sludge in the culture tank;

[0021] preparing the nutrient medium in a nutrient medium storage tank;

[0022] Start the circulation pump to allow the nutrient medium to circulate in the culture tank through the liquid inlet and liquid outlet of the culture tank;

[0023] Observe the growth of the colonies on the carrier, and replace the carrier according to the growth of the colonies to carry out the next round of enrichment.

[0024] According to some embodiments of the present application, the inoculated sludge concentration is 4000-5000 mg / L.

[0025] According to some embodiments of the present application, the nutrient matrix includes: methanol 80-160 mg / L, NH4 + -N: 35-50mg / L, NO2-N: 35-65mg / L, sodium bicarbonate: 300-500mg / L, calcium chloride: 120-180mg / L, potassium dihydrogen phosphate: 15-25mg / L, leucine 0-5mg / L, lysine, 0-2mg / L, aspartic acid 0-2mg / L, glutamic acid, 0-2mg / L, trace elements: 0-5mg / L.

[0026] According to some embodiments of the present application, the hourly circulation volume of the nutrient medium is 1-5 times the volume of water in the culture tank.

[0027] According to some embodiments of the present application, the temperature of the culture tank is 30-35°C, and the pH is 7.8-8.

[0028] According to some embodiments of the present application, the concentrations of ammonia nitrogen and nitrite nitrogen in the effluent from the culture tank are controlled at 30-50 mg / L.

[0029] Compared with the prior art, this application has at least the following beneficial effects:

[0030] The present application provides an enrichment device for anaerobic ammonia-oxidizing bacteria, in which a carrier diaphragm is fixed in a culture tank, and the suitable growth environment for anaerobic ammonia-oxidizing bacteria is accurately simulated by precisely controlling the circulation flow of the mud-water mixture, the flushing amount on the carrier surface, and the composition and concentration of the nutrient matrix, thereby accelerating the growth and enrichment of the anaerobic ammonia-oxidizing bacteria; at the same time, a nozzle flushing method is adopted instead of an immersion method, so that aged strains can be flushed away, thereby ensuring the renewal of the anaerobic ammonia-oxidizing strains on the carrier, so that the inoculated colonies are all viable strains that are not easy to fall off.

[0031] The enrichment device of anaerobic ammonia-oxidizing bacteria of the present application is independent of the sewage treatment system, and is highly efficient in enrichment, thereby avoiding the problem of slow growth and reproduction caused by the influence of pollutants in the sewage treatment system during the re-commissioning of anaerobic ammonia-oxidizing bacteria. The present application provides an enrichment method of anaerobic ammonia-oxidizing bacteria, which is simple and easy to implement. During the enrichment process, the carrier membrane can be replaced at any time according to the growth of the colony, so as to achieve continuous and stable operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a schematic diagram of the structure of an enrichment device according to an exemplary embodiment of the present application.

[0033] Explanation of symbols: 1. Carrier; 2. Culture tank; 3. Nozzle; 4. Circulation pump; 5. Nutrient dissolution tank; 6. Nutrient matrix storage tank; 7. Nutrient transfer pump; 8. Nutrient metering pump; 9. Pipeline mixer; 10. External discharge pump. DETAILED DESCRIPTION

[0034] The technical solution of the present application will be clearly and completely described below in conjunction with the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present application.

[0035] It is particularly important to point out that similar substitutions and modifications made to the present application are obvious to those skilled in the art, and they are all deemed to be included in the present application. Relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the present application to implement and apply the technology of the present application. Obviously, the described embodiments are only some embodiments of the present application, not all embodiments.

[0036] If no specific conditions are specified in this application, the preparation shall be carried out under conventional conditions or the conditions recommended by the manufacturer. The raw materials or excipients used, as well as the reagents or instruments used, for which the manufacturers are not specified, are all conventional products that can be obtained commercially.

[0037] This application provides Figure 1 The enrichment device of an anaerobic ammonia-oxidizing bacteria shown includes: 1. a carrier; 2. a culture tank; 3. a nozzle; 4. a circulation pump; 5. a nutrient dissolution tank; 6. a nutrient matrix storage tank; 7. a nutrient transfer pump; 8. a nutrient metering pump; 9. a pipeline mixer; and 10. an external discharge pump.

[0038] The nutrient dissolving tank is connected to the nutrient matrix storage tank through a nutrient transfer pump; the nutrient matrix storage tank is connected to the pipeline mixer through a nutrient metering pump;

[0039] The pipeline mixer is connected to the nozzle of the liquid inlet of the culture tank through a pipeline;

[0040] A carrier and a nutrient matrix are arranged inside the culture tank.

[0041] The attachment method using the above-mentioned anaerobic ammonia-oxidizing bacteria enrichment device of the present application comprises the following steps:

[0042] The carrier is fixed in a culture tank, and activated sludge rich in anaerobic ammonium oxidation is inoculated into the culture tank;

[0043] The activated sludge-water mixture in the culture tank flows back to the top of the culture tank through a circulation pump, and then is evenly sprayed onto the carrier through a nozzle on the top of the culture tank.

[0044] The nutrient components of the nutrient matrix and water are placed in a nutrient matrix dissolving tank, and the nutrient matrix after stirring and dissolving is transferred to a nutrient matrix storage tank through a nutrient transfer pump.

[0045] The nutrient solution matrix in the nutrient matrix storage tank is transported to the pipeline mixer through a nutrient metering pump and enters the nozzle together with the mud and water mixture.

[0046] After the carrier has completed the enrichment of anaerobic ammonia-oxidizing bacteria, the carrier is taken out for use. Then a new carrier is fixed in the culture tank, and the enrichment can be directly carried out without inoculating sludge again, and the cycle is repeated.

[0047] The culture tank is in a closed state; the interior is an anaerobic environment; the liquid level in the culture tank is lower than the lower position of the carrier.

[0048] When the liquid level is too high or some aged sludge needs to be discharged, a very small amount is discharged through the external discharge pump to achieve dynamic balance.

[0049] The application is described in detail below.

[0050] Example 1

[0051] Use Figure 1 The apparatus shown is used to enrich anaerobic ammonium oxidizing bacteria through the following steps.

[0052] The carrier is fixed in the culture tank, and the activated sludge rich in anaerobic ammonium oxidation is inoculated into the culture tank; the activated sludge and water mixture in the culture tank is refluxed to the top of the culture tank through the circulation pump, and then evenly sprayed onto the carrier through the nozzle on the top of the culture tank; the nutrient components of the nutrient matrix and water are placed in the nutrient matrix dissolution tank, and the nutrient matrix after stirring and dissolving is transferred to the nutrient matrix storage tank through the nutrient transfer pump; the nutrient solution matrix in the nutrient matrix storage tank is transported to the pipeline mixer through the nutrient metering pump, and enters the nozzle together with the sludge and water mixture. After the carrier completes the enrichment of anaerobic ammonium oxidation bacteria, the carrier is taken out.

[0053] Target bacteria: anaerobic ammonia-oxidizing bacteria;

[0054] Inoculated sludge: 10 tons of mixed sludge of anaerobic ammonium oxidizing bacteria and activated sludge from industrial park sewage treatment plants, with a moisture content of 97% and an anaerobic ammonium oxidizing bacteria content of 10%;

[0055] Inoculation sludge concentration: 4000-5000mg / L

[0056] Culture tank group: 10 groups;

[0057] Carrier specification: 15 pieces / can;

[0058] Nutrient matrix: methanol 100mg / L, NH4 + -N: 40mg / L, NO2-N: 40mg / L, sodium bicarbonate: 3000mg / L, calcium chloride: 150mg / L, potassium dihydrogen phosphate: 15mg / L, leucine 1mg / L, lysine, 1mg / L, aspartic acid 1mg / L, glutamic acid, 1mg / L;

[0059] Bacteria enrichment setting temperature: 30℃;

[0060] Bacteria enrichment control pH: 7.5;

[0061] Circulation pump return flow of each tank group: 2m 3 / h;

[0062] Bacteria enrichment time: 100 days

[0063] Results: The total weight of the biofilm on the carrier was 12.7 tons; the water content of the biofilm was 61%; the average content of anaerobic ammonia-oxidizing bacteria was 55%.

[0064] Example 2

[0065] Use Figure 1 The device shown is used to enrich anaerobic ammonia-oxidizing bacteria, and the steps are the same as those in Example 1.

[0066] Target bacteria: anaerobic ammonia-oxidizing bacteria;

[0067] Inoculated sludge: 10 tons of mixed sludge of anaerobic ammonium oxidizing bacteria and activated sludge from industrial park sewage treatment plant, with a moisture content of 97% and an anaerobic ammonium oxidizing bacteria content of 40%;

[0068] Inoculation sludge concentration: 4000-5000mg / L

[0069] Culture tank group: 10 groups;

[0070] Carrier specification: 15 pieces / can;

[0071] Nutrient matrix: methanol 200mg / L, NH4 + -N: 35mg / L, NO2-N: 35mg / L, Sodium bicarbonate: 3000mg / L, Calcium chloride: 150mg / L, Potassium dihydrogen phosphate: 15mg / L, Fe 3+ :2mg / L;

[0072] Bacteria enrichment setting temperature: 30℃;

[0073] Bacteria enrichment control pH: 7.0-7.5;

[0074] Circulation pump return flow of each tank group: 1.5m 3 / h;

[0075] Bacteria enrichment time: 60 days

[0076] Results: The total weight of the biofilm on the carrier was 11.4 tons; the water content of the biofilm was 61%; the average content of anaerobic ammonia-oxidizing bacteria was 57%.

[0077] It is known that the debugging of conventional anaerobic ammonium oxidation wastewater biochemical treatment system, the input sludge bacteria situation is similar to Example 1, anaerobic ammonium oxidation bacteria and sewage treatment plant activated sludge mixed sludge, anaerobic ammonium oxidation bacteria content 10%; then use clean water to start, gradually increase the sewage treatment capacity, generally to reach a stable anaerobic ammonium oxidation state requires 8-18 months.

[0078] According to the data of the above embodiments, it can be seen that the use of the device of the present application can shorten the culture cycle of anaerobic ammonia-oxidizing bacteria, and can be carried out simultaneously with the project construction and installation, without the need to wait until all installation work is completed before debugging and strain enrichment.

[0079] The description of the above embodiments is only used to help understand the method and core idea of ​​the present application. It should be noted that, for ordinary technicians in this technical field, several improvements and modifications can be made to the present application without departing from the principles of the present application, and these improvements and modifications also fall within the scope of protection of the claims of the present application.

Claims

1. An anaerobic ammonium oxidizing bacteria enrichment device, characterized in that: It includes a pretreatment unit, a circulation unit, and an enrichment unit connected in sequence; Wherein, the enrichment unit comprises: one or more culture tanks, and a carrier fixed in each tank body of the one or more culture tanks; Each of the one or more culture tanks is provided with a liquid inlet and a liquid outlet on its tank body, and the liquid inlet is provided with a nozzle; The one or more culture tanks are connected in sequence; Each of the one or more culture tanks is filled with a nutrient medium; The nutrient substrate circulates in the one or more culture tanks to form an aerobic environment or an anaerobic environment.

2. The enrichment device according to claim 1, characterized in that: The carrier is a polyvinylidene chloride membrane.

3. The enrichment device according to claim 2, characterized in that: The number of the polyvinylidene chloride membranes is 2 or more.

4. The enrichment device according to any one of claims 2-3, characterized in that: The polyvinylidene chloride membrane has a mesh structure.

5. The enrichment device according to claim 4, characterized in that: The circulation unit comprises a circulation pump for circulating the nutrient matrix, an exhaust pump for exhausting the nutrient matrix, and a pipeline mixer.

6. The enrichment device according to claim 5, characterized in that: The pretreatment unit comprises: a nutrient dissolving tank, a nutrient matrix storage tank, a nutrient transfer pump, and a nutrient metering pump.

7. The enrichment device according to claim 6, characterized in that: The nutrient dissolving tank is connected to the nutrient matrix storage tank via the nutrient transfer pump.

8. The enrichment device according to claim 7, characterized in that: The nutrient substrate storage tank is connected to the pipeline mixer via the nutrient metering pump.

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