Soil organic carbon mineralization culture bottle

Abstract

The utility model relates to the field of experimental devices, in particular to a soil organic carbon mineralization culture bottle which comprises a container and a cover body, the container is detachably connected with the cover body, the inner bottom of the container protrudes to form an alkali liquor absorption bottle limiting ring, and an alkali liquor absorption bottle is detachably arranged in an inner cavity of the alkali liquor absorption bottle limiting ring. A soil containing cavity is formed between the outside of the limiting ring of the alkali liquor absorption bottle and the circumferential wall of the container ring. The space in the container is divided into the alkali liquor absorption bottle in the alkali liquor absorption bottle limiting ring and the soil containing cavity outside the alkali liquor absorption bottle limiting ring through the alkali liquor absorption bottle limiting ring, and the alkali liquor absorption bottle is used for containing CO2 absorption solution, particularly alkali liquor (such as sodium hydroxide solution). The soil containing cavity outside the alkali liquor absorption bottle limiting ring is used for containing soil to be detected, the overall size is small, and the structure is compact.

Description

Technical Field

[0001] The utility model relates to the field of experimental devices, in particular to a soil organic carbon mineralization culture bottle. Background Art

[0002] In the field of soil science and environmental research, it is of great significance to have a deep understanding of the mineralization process of soil organic carbon. The mineralization of soil organic carbon not only affects soil fertility and the carbon cycle of the ecosystem, but is also closely related to climate change. The traditional soil organic carbon mineralization culture test is to put a certain mass of processed soil samples into a culture container, spread the soil evenly on the bottom of the bottle, and then place the culture bottle in a constant temperature incubator with a set temperature for culture. During the culture process, the absorption liquid absorbs the CO2 released by soil organisms, and then uses hydrochloric acid standard solution for titration. The volume of hydrochloric acid consumed characterizes the rate of soil organic carbon mineralization. At present, the more advanced methods for studying soil organic carbon mineralization are gas chromatography and infrared gas analyzer. Gas chromatography is mainly suitable for small sample determination, that is, detecting the amount of CO2 released by the sample in a short time (1-2 min); while the infrared gas analyzer method is to set up an open gas path system through a computer to establish a linear relationship between the change of CO2 concentration and time in the system to calculate the mineralization of organic carbon. The above test measurement requires equipment with complex structure and operation, which is inconvenient to operate and not easily accepted by people. In addition, the equipment cost is high. Once a failure occurs, the repair time is long and the maintenance cost is high.

[0003] Patent CN201621169745.1 discloses an indoor device for measuring soil organic carbon mineralization, including a plastic outer cover, a rubber inner plug, a CO2 absorption cup, a soil sample incubator and a brown plastic bottle, wherein the CO2 absorption cup and the soil sample incubator are placed in a brown plastic bottle, the rubber inner plug plugs the mouth of the brown plastic bottle, and the plastic outer cover is nested on the upper part of the rubber inner plug and tightly buckled to seal the mouth of the brown plastic bottle. A 2 L brown honey bottle with a larger diameter is used, and the CO2 absorption cup is filled with alkali solution and the soil sample incubator is filled with soil and a certain amount of water, and then placed in the bottle together, and sealed with a rubber inner plug and a plastic outer cover. This structure is large in size and is not suitable for simultaneous measurement of multiple soil samples. Utility Model Content

[0004] The utility model aims to overcome the shortcomings and deficiencies of the prior art and to provide a soil organic carbon mineralization culture bottle.

[0005] The technical effects adopted by the utility model are as follows: a soil organic carbon mineralization culture bottle, which includes a container and a cover body, the container and the cover body are detachably connected, the bottom protrusion of the container is formed with a limiting ring for an alkali liquid absorption bottle, the inner cavity of the limiting ring for the alkali liquid absorption bottle is detachably provided with an alkali liquid absorption bottle, and a soil containing cavity is formed between the outside of the limiting ring for the alkali liquid absorption bottle and the circumferential wall of the container.

[0006] The limiting ring of the alkali solution absorption bottle is arranged at the center of the container.

[0007] The upper end surface of the cover body is concave to form a matching groove, and at least two first limit blocks are protruding inward from the upper end of the matching groove and distributed at equal intervals, a slot is formed between adjacent first limit blocks, and a limit rotation groove is formed between the first limit block and the bottom surface of the matching groove;

[0008] The bottom of the container is convex to form a matching block adapted to the matching groove, and the lower end of the matching block is circumferentially convex with a second limiting block, the second limiting block is adapted to the slot and the thickness of the second limiting block is adapted to the height of the limiting rotating groove.

[0009] There are three first limit blocks and three second limit blocks.

[0010] A sealing member is provided between the container and the cover body.

[0011] The container and the cover body are connected via a snap assembly.

[0012] The buckle assembly comprises a connecting piece fixed on the container, a fixing piece fixed on the cover body and a flip piece rotatably connected to the connecting piece.

[0013] The flip member includes a first rotating member hinged on the connecting member and a second rotating member hinged on the first rotating member, the end of the second rotating member forms a buckle plate opening, and the end of the fixing member forms a buckle hook that cooperates with the buckle plate opening.

[0014] The flipping members are provided in two groups and are arranged at two opposite sides of the container, and the fixing members are provided in two groups and are arranged at two opposite sides of the cover body.

[0015] The beneficial effects of the utility model are as follows: the utility model divides the space in the container into an alkali liquid absorption bottle inside the alkali liquid absorption bottle limiting ring and a soil containing cavity outside the alkali liquid absorption bottle limiting ring through the alkali liquid absorption bottle limiting ring; the alkali liquid absorption bottle is used for loading CO2 absorption solution, specifically alkali liquid (such as sodium hydroxide solution); the soil containing cavity outside the alkali liquid absorption bottle limiting ring is used for loading soil to be tested; the overall volume is small and the structure is compact. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, without paying creative labor, other drawings obtained based on these drawings still belong to the scope of the utility model.

[0017] Figure 1This is a schematic diagram of the structure of an embodiment of the utility model;

[0018] Figure 2 A cross-sectional view of an embodiment of the utility model;

[0019] Figure 3 This is a schematic diagram of the three-dimensional structure of a container according to an embodiment of the utility model;

[0020] Figure 4 A bottom view of a container according to an embodiment of the utility model;

[0021] Figure 5 This is a schematic diagram of the structure of a cover body according to an embodiment of the utility model;

[0022] Figure 6 Two stacking matching schematic diagrams of an embodiment of the utility model;

[0023] Figure 7 for Figure 2 The enlarged schematic diagram of part A in the middle;

[0024] In the figure, container-1, alkali liquid absorption bottle limiting ring-101, matching block-102, second limiting block-103, cover body-2, matching groove-201, first limiting block-202, slot-203, limiting rotation groove-204, alkali liquid absorption bottle-3, connecting member-401, fixing member-402, first rotating member-403, second rotating member-404, buckle plate mouth-405, buckle hook-406, sealing member-5. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solutions and advantages of the utility model clearer, the utility model will be further described in detail below with reference to the accompanying drawings.

[0026] It should be noted that all expressions using "first" and "second" in the embodiments of the present invention are for distinguishing two non-identical entities with the same name or non-identical parameters. It can be seen that "first" and "second" are only for the convenience of expression and should not be understood as limitations on the embodiments of the present invention. The subsequent embodiments will not explain this one by one.

[0027] The directions and positions mentioned in the present invention, such as up, down, front, back, left, right, inside, outside, top, bottom, side, etc., are only directions or positions with reference to the drawings. Therefore, the directions and positions used are used to explain and understand the present invention, but not to limit the scope of protection of the present invention.

[0028] A soil organic carbon mineralization culture bottle, such as Figure 1 , Figure 2 As shown, it comprises a container 1 and a cover 2, wherein the container 1 and the cover 2 are detachably connected. Figure 3 As shown, the bottom of the container 1 is convexly formed with an alkali liquid absorption bottle limiting ring 101, and the inner cavity of the alkali liquid absorption bottle limiting ring 101 can be detachably provided with an alkali liquid absorption bottle 3, and a soil holding cavity is formed between the outer portion of the alkali liquid absorption bottle limiting ring 101 and the circumferential wall of the container 1. The space in the container is divided into the alkali liquid absorption bottle 3 inside the alkali liquid absorption bottle limiting ring 101 and the soil holding cavity outside the alkali liquid absorption bottle limiting ring 101 by the alkali liquid absorption bottle limiting ring. The alkali liquid absorption bottle 3 is used to load the CO2 absorption solution, specifically alkali liquid (such as sodium hydroxide solution), and the soil holding cavity outside the alkali liquid absorption bottle limiting ring 101 is used to load the soil to be tested. The overall volume is small and the structure is compact. Among them, the container 1 and the cover body 2 can be made of plastic or glass, and the alkali liquid absorption bottle 3 can be a common beaker.

[0029] When used in this embodiment, the test process is roughly as follows: The soil organic carbon mineralization culture test adopts the indoor constant temperature culture alkali solution absorption method. Soil sample Weigh fresh soil equivalent to 20 g of dry soil weight and place it in the soil holding cavity of the culture bottle of this embodiment. Adjust the soil moisture content to 60% of the field water holding capacity (WHC), seal it and place it in a 25 ℃ constant temperature incubator for pre-culture for 1 day to restore the activity of soil microorganisms. Then put 10 mL of 0.5 mol L －1 The NaOH solution absorption bottle (i.e., the alkali solution absorption bottle 3 in this embodiment) was placed in the culture bottle, sealed and placed in a 25°C constant temperature incubator for culture. The alkali solution absorption bottle was replaced on the 1st, 3rd, 5th, 7th, 14th, 28th, and 42nd day after culture, and the alkali solution in the replaced bottle was titrated. The amount of soil organic carbon mineralization (mg C·kg －1 ) .

[0030] Furthermore, the alkali solution absorption bottle limiting ring 101 is arranged at the center of the container 1 .

[0031] Further, such as Figure 5 As shown, the upper end surface of the cover body 2 is concave to form a matching groove 201, and at least two first limit blocks 202 with equal spacing protrude inward from the upper end of the matching groove 201, a slot 203 is formed between adjacent first limit blocks 202, and a limit rotation groove 204 is formed between the first limit block 202 and the bottom surface of the matching groove 201; Figure 4As shown, the bottom of the container 1 is raised to form a matching block 102 adapted to the matching groove 201, and the lower end of the matching block 102 is circumferentially raised with a second limit block 103, and the second limit block 103 is adapted to the slot 203 and the thickness of the second limit block 103 is adapted to the height of the limit rotation slot 204. Through the above-mentioned structural setting, the second limit block 103 can be inserted into the matching groove 201 through the slot 203, and then rotated into the limit rotation slot 204 between the first limit block 202 and the bottom surface of the matching groove 201, and the culture bottles can be connected and stacked longitudinally, and moved into / taken out of the constant temperature incubator for operation. Generally speaking, the soil organic carbon mineralization culture test will involve the rhizosphere and non-rhizosphere tests of multiple plants in multiple test sites. At the same time, each soil needs to set up multiple groups of repeated test groups, so a large number of test groups will be involved and need to be tested at the same time. The culture bottles of this embodiment can be stacked longitudinally, which can save space in the constant temperature incubator, and can form a stable connection relationship, and are not easy to collapse when moved in / out.

[0032] Furthermore, three first limit blocks 202 are provided, and three second limit blocks 103 are provided to improve connection stability.

[0033] Further, such as Figure 7 As shown, a sealing member 5 is provided between the container 1 and the cover body 2 . Specifically, the sealing member 5 is an O-ring sleeved on the outer periphery of the cover body 2 and is sealed with the inner wall of the container 1 .

[0034] Furthermore, the container 1 and the cover body 2 are connected via a snap assembly 4 .

[0035] Further, such as Figure 7 As shown, the buckle assembly 4 includes a connecting member 401 fixed on the container 1, a fixing member 402 fixed on the cover 2, and a flip member rotatably connected to the connecting member 401. The flip member includes a first rotating member 403 hinged on the connecting member 401 and a second rotating member 404 hinged on the first rotating member 403, the end of the second rotating member 404 forms a buckle plate opening 405, and the end of the fixing member 402 forms a buckle hook 406 that cooperates with the buckle plate opening 405. The flip member is provided in two groups and is provided on two opposite sides of the container 1, and the fixing member 402 is provided in two groups and is provided on two opposite sides of the cover 2.

[0036] The above disclosure is only a preferred embodiment of the present invention, and certainly cannot be used to limit the scope of rights of the present invention. Therefore, equivalent changes made according to the claims of the present invention are still within the scope covered by the present invention.

Claims

1. A soil organic carbon mineralization culture bottle, comprising a container (1) and a cover (2), wherein the container (1) and the cover (2) are detachably connected, and characterized in that: The bottom of the container (1) is raised to form an alkali solution absorption bottle limiting ring (101), the inner cavity of the alkali solution absorption bottle limiting ring (101) is detachably provided with an alkali solution absorption bottle (3), and a soil containing cavity is formed between the outside of the alkali solution absorption bottle limiting ring (101) and the circumferential wall of the container (1).

2. The soil organic carbon mineralization culture bottle according to claim 1, characterized in that: The alkali solution absorption bottle limiting ring (101) is arranged at the center of the container (1).

3. The soil organic carbon mineralization culture bottle according to claim 1, characterized in that: The upper end surface of the cover body (2) is concave to form a matching groove (201), and at least two first limiting blocks (202) are protruding inward from the upper end of the matching groove (201) and are distributed at equal intervals, a slot (203) is formed between adjacent first limiting blocks (202), and a limiting rotation groove (204) is formed between the first limiting blocks (202) and the bottom surface of the matching groove (201); The bottom of the container (1) is raised to form a matching block (102) adapted to the matching groove (201); a second limiting block (103) is circumferentially raised at the lower end of the matching block (102); the second limiting block (103) is adapted to the slot (203) and the thickness of the second limiting block (103) is adapted to the height of the limiting rotation groove (204).

4. The soil organic carbon mineralization culture bottle according to claim 3, characterized in that: There are three first limit blocks (202) and three second limit blocks (103).

5. The soil organic carbon mineralization culture bottle according to claim 1, characterized in that: A sealing member (5) is provided between the container (1) and the cover body (2).

6. The soil organic carbon mineralization culture bottle according to claim 1, characterized in that: The container (1) and the cover body (2) are connected via a snap assembly (4).

7. The soil organic carbon mineralization culture bottle according to claim 6, characterized in that: The buckle assembly (4) comprises a connecting piece (401) fixed on the container (1), a fixing piece (402) fixed on the cover body (2), and a flip piece rotatably connected to the connecting piece (401).

8. The soil organic carbon mineralization culture bottle according to claim 7, characterized in that: The flip member comprises a first rotating member (403) hinged on the connecting member (401) and a second rotating member (404) hinged on the first rotating member (403), the end of the second rotating member (404) forms a buckling plate opening (405), and the end of the fixing member (402) forms a buckling hook (406) that cooperates with the buckling plate opening (405).

9. The soil organic carbon mineralization culture bottle according to claim 8, characterized in that: The flipping members are provided in two groups and are arranged on two opposite sides of the container (1); the fixing members (402) are provided in two groups and are arranged on two opposite sides of the cover body (2).

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