Ultrafiltration device for enriching microorganisms in water body

Abstract

The utility model discloses an ultrafiltration device for enriching microorganisms in a water body, the ultrafiltration device comprises a tubular shell, a liquid inlet and a water inlet are respectively arranged at two opposite ends of the shell along the length direction, hollow fiber filter membranes are embedded in the liquid inlet and the water inlet, the water inlet is used for introducing a water sample, and the hollow fiber filter membranes are used for introducing a water sample. Wherein the liquid inlet is used for being communicated with an eluent inlet device, a liquid return prevention elastic valve is arranged on the outer side, close to the hollow fiber filter membranes, in the liquid inlet of the shell, a water outlet is formed in the side, close to the liquid inlet, of the shell, and the water outlet is formed between the hollow fiber filter membranes at the two ends of the shell. According to the ultrafiltration device for enriching the microorganisms in the water body, the tubular shell which is of an integrated shell structure is adopted, it can be guaranteed that the interior is kept in a sterile environment to the maximum extent, the risk of bacterial pollution in the filtering process can be reduced, and the filtering effect is better.

Description

Technical Field

[0001] The utility model relates to the technical field of filtering equipment, in particular to an ultrafiltration device for enriching microorganisms in water bodies. Background Art

[0002] With the development of urbanization and population concentration, drinking water safety has become an important issue in densely populated areas. Once the water source is contaminated by infectious organisms, it is very likely to threaten public health safety. Therefore, how to efficiently and quickly enrich and detect microorganisms in water bodies and give early warning of risk pollution is one of the important conditions for reducing public health safety risks.

[0003] At present, ultrafiltration based on hollow fiber membrane is a membrane separation technology with an average pore size between 3-100nm. This membrane can intercept solutes in the solution through screening and the chemical properties of the membrane pore surface, thereby achieving purification, separation and concentration of the solution. When water flows through the surface of the ultrafiltration membrane, only water and low molecular weight solutes can pass through, while solutes larger than the membrane pore size are intercepted.

[0004] However, conventional hollow fiber membrane ultrafiltration devices have a complex structure and are not easy to disassemble and maintain. At the same time, due to poor structural stability, there is also a risk of internal bacterial contamination. Utility Model Content

[0005] One advantage of the utility model is that it provides an ultrafiltration device for enriching microorganisms in water bodies, wherein the use of an integrated shell structure such as a tubular shell can ensure that the internal environment is kept as sterile to the greatest extent, can reduce the risk of bacterial contamination during the filtration process, and achieve a better filtration effect.

[0006] One advantage of the utility model is that it provides an ultrafiltration device for enriching microorganisms in water bodies, wherein an anti-liquid backflow elastic valve is independently arranged at the liquid inlet, which automatically remains in a closed state when not connected, and can effectively prevent the water sample from flowing back.

[0007] One advantage of the utility model is that it provides an ultrafiltration device for enriching microorganisms in water bodies, wherein the elastic pressure of the inner end of the anti-liquid backflow elastic valve can be reduced by designing a flat duckbill structure, making it easier to insert the liquid inlet needle into the liquid inlet cavity and to pull it out without reducing the anti-liquid backflow effect of the anti-liquid backflow elastic valve.

[0008] In order to achieve at least one of the above advantages of the utility model, the utility model provides an ultrafiltration device for enriching microorganisms in water bodies, including a tubular shell, wherein the shell is respectively provided with a liquid inlet and a water inlet at two opposite ends along the length direction, and hollow fiber filter membranes are embedded in the liquid inlet and the water inlet, wherein the water inlet is used to introduce water samples, wherein the liquid inlet is used to connect to an eluent inlet, and the shell is provided with an anti-liquid backflow elastic valve on the outside of the hollow fiber filter membrane in the liquid inlet, and the shell is provided with a water outlet on a side close to the liquid inlet, and the water outlet is located between the hollow fiber filter membranes at both ends.

[0009] According to one embodiment of the utility model, the anti-liquid backflow elastic valve is embedded in the liquid inlet in an interference fit manner, and the anti-liquid backflow elastic valve is provided with an injection channel along the central axis, so that the liquid inlet needle of the eluent inlet device can pass through the injection channel and then be inserted into the liquid inlet cavity between the anti-liquid backflow elastic valve and the adjacent hollow fiber filter membrane, and after the liquid inlet needle is pulled out, the injection channel can be automatically closed based on the elastic properties of the anti-liquid backflow elastic valve.

[0010] According to an embodiment of the utility model, the anti-liquid backflow elastic valve is provided with a limiting spherical structure and a flat duckbill structure at two opposite ends along the length direction, wherein the limiting spherical structure is interference fit with the inner wall of the liquid inlet, the flat duckbill structure is located at one end close to the water inlet, and the injection channel is located at the center of the flat duckbill structure.

[0011] According to an embodiment of the utility model, the anti-liquid-backflow elastic valve is further provided with a fixing ring near the middle, and the inner wall of the liquid inlet is provided with a limiting groove matched with the fixing ring.

[0012] According to an embodiment of the utility model, the water outlet is connected to a Luer female connector.

[0013] According to an embodiment of the utility model, the hollow fiber filter membrane is connected to the inner wall of the shell by an adhesive.

[0014] According to an embodiment of the present invention, the pore size of the hollow fiber filter membrane is 0.01-1.0 micrometer.

[0015] These and other purposes, features and advantages of the present invention are fully reflected in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A schematic structural diagram of an ultrafiltration device for enriching microorganisms in water according to a preferred embodiment of the present application is shown.

[0017] Figure 2A cross-sectional schematic diagram showing an ultrafiltration device for enriching microorganisms in water according to a preferred embodiment of the present application

[0018] Figure 3 A partial enlarged structural schematic diagram of the ultrafiltration device in the present application in conjunction with the eluent inlet device is shown.

[0019] Figure 4 A partial enlarged structural schematic diagram of the ultrafiltration device in the present application in conjunction with the eluent inlet device is shown.

[0020] Figure 5 A schematic diagram of the use process of the ultrafiltration device in the present application is shown. DETAILED DESCRIPTION

[0021] The following description is used to disclose the utility model so that those skilled in the art can implement the utility model. The preferred embodiments described below are only examples, and those skilled in the art can think of other obvious variations. The basic principles of the utility model defined in the following description can be applied to other embodiments, variations, improvements, equivalents, and other technical solutions that do not deviate from the spirit and scope of the utility model.

[0022] Those skilled in the art should understand that, in the disclosure of the specification, the orientation or position relationship indicated by the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. are based on the orientation or position relationship shown in the drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the above terms should not be understood as limiting the present invention.

[0023] It is to be understood that the term "one" should be understood as "at least one" or "one or more", that is, in one embodiment, the number of an element may be one, while in another embodiment, the number of the element may be multiple, and the term "one" should not be understood as a limitation on the quantity.

[0024] refer to Figures 1 to 5According to a preferred embodiment of the utility model, an ultrafiltration device for enriching microorganisms in water will be described in detail below, wherein the ultrafiltration device 100 for enriching microorganisms in water includes a tubular shell 103, and the shell 103 is respectively provided with a liquid inlet 101 and a water inlet 104 at two opposite ends along the length direction, and a hollow fiber filter membrane 105 is embedded in the liquid inlet 101 and the water inlet 104, wherein the hollow fiber filter membrane 105 is a modified hydrophilic membrane filter medium made of modified polyethersulfone (mPES), which can provide a higher filtration flux, wherein polymer particles are formed into hollow fibers in a molten state through a high-speed rotating spinning nozzle, wherein the pore size of the hollow fiber filter membrane 105 is 0.01-1.0 microns. In order to facilitate the installation of the hollow fiber filter membrane 105 and ensure that it has good connection structure stability after installation, the hollow fiber filter membrane 105 is connected to the inner wall of the shell 103 through an adhesive 106, wherein the adhesive 106 is made of polyurethane / epoxy resin. In addition, the water inlet 104 is used to pass the water sample, and the liquid inlet 101 is used to connect the eluent inlet 200. The shell 103 is provided with an anti-return elastic valve 107 on the outside of the hollow fiber filter membrane 105 in the liquid inlet 101, and the shell 103 is provided with a water outlet 102 on one side close to the liquid inlet 101, and the water outlet 102 is between the hollow fiber filter membranes 105 at both ends. For easy installation and quick connection, the water outlet 102 is connected with a Luer female head, which can be quickly adapted to a Luer male head. Therefore, the ultrafiltration device 100 for enriching microorganisms in water provided by the present application adopts an integrated shell structure such as a tubular shell, which can ensure that the internal environment is kept aseptic to the greatest extent, reduce the risk of bacterial contamination during the filtration process, and achieve better filtration effect.

[0025] In one embodiment, the anti-liquid backflow elastic valve 107 is embedded in the liquid inlet 101 in an interference fit manner, and the anti-liquid backflow elastic valve 107 is provided with a liquid injection channel 1074 along the central axis, so that the liquid inlet needle 201 of the eluent inlet 200 can penetrate the liquid injection channel 1074, and then be inserted into the liquid inlet cavity 108 between the anti-liquid backflow elastic valve 107 and the adjacent hollow fiber filter membrane 105, and after the liquid inlet needle 201 is pulled out, based on the elastic properties of the anti-liquid backflow elastic valve 107, the liquid injection channel 1074 can be automatically closed, so that the liquid inlet 101 of the ultrafiltration device 100 is always in a closed state when not connected, which can effectively prevent the backflow of water samples, and in the state of adding liquid through the liquid inlet needle 201, the pressure inside the liquid adding needle 201 is much greater than the pressure in the liquid inlet cavity 108, and the liquid inlet 101 is still maintained in a state of only taking in liquid but not discharging liquid, which can also prevent the backflow of water samples.

[0026] During specific use, the ultrafiltration device 100 provided by the present application is kept in a vertical state, wherein the liquid inlet 101 is connected to the eluent inlet 200, specifically, the liquid inlet needle 201 of the eluent inlet 200 is inserted into the liquid inlet 101, and penetrates the anti-liquid return elastic valve 107 and extends into the liquid inlet cavity 108, wherein the water inlet 104 is connected to the water sample cup 111, and the volume of the water sample is usually 50mL-200mL, more specifically, the water outlet 102 is connected to the water outlet silicone tube 110, and is connected to the waste liquid cylinder 112 through the peristaltic pump 109. The water sample to be enriched passes through the ultrafiltration device 100 in the water sample cup 111, and then passes through the water outlet silicone tube 110 and the peristaltic pump 109 (the speed is set at 300rpm, and the flow rate range is 200mL / min-300mL / min), and is finally connected to the waste liquid cylinder 112. The eluent uses 1mM phosphate buffer Tween 20 solution (Tween 20 is 0.05-0.1% concentration) and Tris-EDTA buffer (Tris 0.05-0.1% concentration), which enters the ultrafiltration device 100 through the liquid inlet 101 from the liquid inlet needle 201 on the eluent inlet 200, and then flows out of the ultrafiltration device 100 through the water inlet 104, and finally collected in a clean container. The volume of the water sample is concentrated from 50mL-100mL to 0.1mL-1.5mL. The time is within 3 minutes, and the enrichment and concentration of microorganisms in the water body are completed.

[0027] In one embodiment, the anti-liquid-backward elastic valve 107 is provided with a limited spherical structure 1071 and a flat duckbill structure 1073 at two opposite ends along the length direction, wherein the limited spherical structure 1071 is interference fit with the inner wall of the liquid inlet 101 to limit the anti-liquid-backward elastic valve 107 in the liquid inlet 101, wherein the flat duckbill structure 1073 is located at one end close to the water inlet 104, and the injection channel 1074 is located at the center of the flat duckbill structure 1073. Position, as a result, the side wall of the flat duckbill structure 1073 is relatively thin, and the elastic squeezing force is relatively weak, so that the liquid inlet needle 201 can be more conveniently inserted into the liquid inlet cavity 108, or pulled out from the liquid inlet cavity 108. In the anti-liquid backflow elastic valve 107, the elastic squeezing force is relatively thin only at the flat duckbill structure 1073, and the elastic squeezing force at other parts is still relatively strong. Therefore, even if the flat duckbill structure 1073 is set, the anti-liquid backflow effect of the anti-liquid backflow elastic valve 107 will not be reduced or affected.

[0028] Further preferably, the anti-liquid backflow elastic valve 107 is also provided with a fixing ring 1072 near the middle, and the inner wall of the liquid inlet 101 is provided with a limiting groove matching with the fixing ring 1072, so that the stability of the anti-liquid backflow elastic valve 107 in the shell 103 can be further improved through the cooperation of the fixing ring 1072 and the limiting groove.

[0029] It should be understood by those skilled in the art that the embodiments of the present invention described above and shown in the accompanying drawings are only examples and do not limit the present invention. The advantages of the present invention have been fully and effectively realized. The functions and structural principles of the present invention have been demonstrated and explained in the embodiments, and the embodiments of the present invention may be deformed or modified in any way without departing from the principles.

Claims

1. An ultrafiltration device for enriching microorganisms in water, characterized in that: It comprises a tubular shell, wherein the two opposite ends of the shell along the length direction are respectively provided with a liquid inlet and a water inlet, and hollow fiber filter membranes are embedded in the liquid inlet and the water inlet, wherein the water inlet is used to introduce water samples, wherein the liquid inlet is used to connect to an eluent inlet, and the shell is provided with an anti-liquid return elastic valve on the outside of the hollow fiber filter membrane in the liquid inlet, and the shell is provided with a water outlet on one side close to the liquid inlet, and the water outlet is located between the hollow fiber filter membranes at both ends.

2. The ultrafiltration device for enriching microorganisms in water according to claim 1, characterized in that: The anti-liquid backflow elastic valve is embedded in the liquid inlet in an interference fit manner, and the anti-liquid backflow elastic valve is provided with an injection channel along the central axis, so that the liquid inlet needle of the eluent inlet device can pass through the injection channel and then be inserted into the liquid inlet cavity between the anti-liquid backflow elastic valve and the adjacent hollow fiber filter membrane, and after the liquid inlet needle is pulled out, the injection channel can be automatically closed based on the elastic properties of the anti-liquid backflow elastic valve.

3. The ultrafiltration device for enriching microorganisms in water as claimed in claim 2, characterized in that: The anti-liquid backflow elastic valve is provided with a limiting spherical structure and a flat duckbill structure at two opposite ends along the length direction, wherein the limiting spherical structure is interference fit with the inner wall of the liquid inlet, the flat duckbill structure is located at one end close to the water inlet, and the injection channel is located at the center of the flat duckbill structure.

4. The ultrafiltration device for enriching microorganisms in water as claimed in claim 3, characterized in that: The anti-liquid-backward elastic valve is also sleeved with a fixing ring near the middle, and the inner wall of the liquid inlet is provided with a limiting groove matched with the fixing ring.

5. The ultrafiltration device for enriching microorganisms in water according to claim 1, characterized in that: The water outlet is connected with a Luer female connector.

6. The ultrafiltration device for enriching microorganisms in water according to claim 1, characterized in that: The hollow fiber filter membrane is connected to the inner wall of the shell through an adhesive.

7. The ultrafiltration device for enriching microorganisms in water according to claim 6, characterized in that: The pore size of the hollow fiber filter membrane is 0.01-1.0 micron.

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