Flow antibody management device

Abstract

The utility model relates to the technical field of reagent management, and discloses a flow antibody management device which comprises a freezing box, a freezing cavity is arranged in the freezing box, a sealing door is connected to the front end of the freezing box in an openable and closable mode, the flow antibody management device further comprises a supporting frame, and the supporting frame is embedded in the freezing cavity in a sliding mode. The supporting frame comprises a box body defined by a bottom wall and four side walls, one end of the box body is open, the open end of the box body faces the left side or the right side of the freezing box, and a plurality of transverse plates and vertical plates are fixedly arranged in the supporting frame so that the inner space of the supporting frame can be divided into a plurality of supporting cavities. A driving assembly used for driving the supporting frame to move is arranged on the freezing box, a rack is fixedly arranged on one side of the supporting frame in the front-back direction of the freezing box, the driving assembly comprises a motor, the motor is fixedly installed on the inner wall of the freezing cavity, and a gear in meshing transmission with the rack is fixed to the output end of the motor. The problem that corresponding antibodies cannot be automatically taken by existing flow type antibody management equipment is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of reagent management, in particular to a flow antibody management device. Background Art

[0002] Flow cytometry antibodies usually refer to monoclonal antibodies against cell surface antigens that are labeled with fluorescent dyes. When the labeled fluorescent dyes are large molecular fluorescent proteins, such as phycoerythrin and allophycocyanin, flow cytometry antibodies are not suitable for storage at low temperatures of -20°C, because the -20°C environment will freeze the antibody-dye coupling molecules, destroy their fluorescent light-emitting groups or cause changes in the antibody structure. Therefore, flow cytometry antibodies are generally stored in a 2-8°C refrigerator in a liquid state.

[0003] Existing flow cytometry antibody storage is usually performed by placing reagent tubes containing flow cytometry antibodies on a reagent rack, which is placed on a support frame. The support frame is slidably arranged in the freezer chamber of a refrigerator. When taking out antibodies, the user needs to manually pull out the support frame and then remove the reagent rack from the support frame. Existing flow cytometry antibody management equipment has relatively single functions and cannot automatically take out corresponding antibodies. During the taking process, there may be a risk of antibody activity reduction or contamination due to improper operation. Utility Model Content

[0004] In view of this, the purpose of the utility model is to provide a flow cytometry antibody management device to solve the problem that the existing flow cytometry antibody management equipment has a relatively single function and cannot automatically obtain the corresponding antibodies, and there is a risk of antibody activity reduction or contamination due to improper operation during the retrieval process.

[0005] The utility model solves the above technical problems through the following technical means: a flow antibody management device, including a freezer, a freezer chamber is arranged inside the freezer, a front end of the freezer is openably connected to a sealed door, and also includes a support frame, the support frame is slidably embedded in the freezer chamber, the support frame includes a box body with one end open formed by a bottom wall and four side walls, the open end of the box body faces the left side or the right side of the freezer, a plurality of horizontal plates and vertical plates are fixedly arranged inside the support frame to divide the internal space of the support frame into a plurality of support chambers, a drive assembly for driving the support frame to move is arranged on the freezer, a rack is fixedly arranged on one side of the support frame along the front-rear direction of the freezer, the drive assembly includes a motor, the motor is fixedly mounted on the inner wall of the freezer chamber, and a gear meshing with the rack is fixed at the output end of the motor.

[0006] Furthermore, a lower cavity is provided inside the freezer below the freezing cavity, and a partition is vertically provided on the inner wall of the lower cavity, and the partition divides the lower cavity into an inner moisturizing cavity and an outer installation cavity, a moisturizing layer is provided in the moisturizing cavity, and the drive assembly is installed in the installation cavity.

[0007] Furthermore, a connecting frame is fixed to the inner end surface of the supporting frame, a cavity is provided inside the connecting frame, the top of the connecting frame is closed, the bottom of the connecting frame is open and faces the moisturizing cavity, a through hole is provided on the top wall of the moisturizing cavity, the moisturizing cavity and the cavity are connected through the through hole, a first through hole is provided on the side wall of the supporting frame at the inner end and each vertical plate inside the supporting frame, a second through hole is provided on the side wall of the supporting frame facing the position corresponding to the first through hole, and the supporting cavity and the cavity are connected through the first through hole and the second through hole.

[0008] Furthermore, a mounting groove is provided in the middle position of the bottom of the support frame along the front-to-rear direction of the freezer, the rack is fixed in the mounting groove, the motor is fixed to the inner wall of the mounting cavity, a connecting groove is provided on the top wall of the mounting cavity, the mounting cavity and the freezer cavity are connected through the connecting groove, and the top end of the gear extends out of the connecting groove and meshes with the rack for transmission.

[0009] Furthermore, the top and bottom ends of the support frame are respectively provided with dovetail grooves, and the inner wall of the freezing chamber is provided with dovetail blocks matching the dovetail grooves.

[0010] Furthermore, there are multiple freezing cavities, which are distributed on the freezer in a linear array. The freezer is provided with a temperature control system for controlling and adjusting the temperature of each freezing cavity.

[0011] The beneficial effects of the utility model are:

[0012] 1. In the utility model, the motor rotates, driving the gear to rotate, and the gear drives the rack and the support frame to move along the front and rear direction of the freezer, so that the corresponding antibodies can be automatically taken when needed, which greatly reduces the need for manual operation and reduces the risk of antibody activity reduction or contamination due to improper operation.

[0013] 2. By dividing the internal space of the support frame into multiple support cavities, each support cavity can be used to place a reagent rack, so that multiple reagent racks and reagent tubes can be placed, which is convenient for batch storage of flow cytometry antibodies. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The present invention can be further described by the non-limiting embodiments given in the accompanying drawings;

[0015] Figure 1 It is a structural schematic diagram of a flow cytometry antibody management device of the utility model;

[0016] Figure 2 This is a structural schematic diagram of a support frame in a flow antibody management device of the utility model;

[0017] Figure 3 yes Figure 2 A schematic diagram of the enlarged structure at A in the middle;

[0018] Figure 4 It is a cross-sectional view of a support frame in a flow antibody management device of the utility model;

[0019] Figure 5 yes Figure 4 Schematic diagram of the enlarged structure at point B in the middle.

[0020] In the above drawings: 1. freezer; 2. support frame; 3. connecting frame; 4. moisturizing cavity; 5. moisturizing layer; 6. mounting cavity; 7. horizontal plate; 8. vertical plate; 9. cavity; 10. through hole; 11. gear; 12. rack; 13. connecting groove; 14. mounting groove; 15. dovetail groove; 16. dovetail block; 17. first perforation; 18. second perforation; 19. control panel; 20. reagent tube; 21. reagent rack. DETAILED DESCRIPTION

[0021] The following describes the implementation of the present invention through specific embodiments. Those skilled in the art can understand the advantages and effects of the present invention from the contents disclosed in this specification. It should be noted that the illustrations provided in the following embodiments are only for exemplary descriptions, and they are only schematic diagrams, not physical diagrams, and cannot be understood as limitations on the present invention. In order to better illustrate the embodiments of the present invention, some parts in the figures may be omitted, enlarged or reduced, and do not represent the size of the actual product; for those skilled in the art, it is understandable that some well-known structures and their descriptions in the figures may be omitted.

[0022] The same or similar numbers in the figures of the embodiments of the present invention correspond to the same or similar parts. In the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right", "front", "back", etc. indicate the orientation or position relationship, they are based on the orientation or position relationship shown in the figure. This is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the terms describing the position relationship in the figures are only used for illustrative purposes and cannot be understood as limitations on the present invention. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to the specific circumstances. In the description of this application, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0023] like Figure 1-5 As shown, the embodiment of the utility model proposes a flow antibody management device, including a freezer 1, the middle part of the freezer 1 is provided with six freezing chambers in a linear array in the horizontal direction, and the front end of the freezer 1 can be opened and closed and connected with a sealed door (not shown in the figure), and the sealed door is used to seal the freezing chamber, which is a common technology of the refrigeration module, and no further elaboration is made here. A high-precision temperature control system is provided on the freezer 1, and the temperature control system is used to control and adjust the temperature of each freezing chamber. Specifically, the temperature control system includes a sensor, a control panel 19, an actuator and a power supply, wherein the sensor includes a temperature sensor, a humidity sensor and other sensing devices to sense the actual temperature and humidity changes, and the control panel 19 is provided on the top front of the freezer 1, which is convenient for the user to operate, which is a common technology of the temperature control module, and no further elaboration is made here. With such a structural design, a variety of different flow antibodies can be placed inside the device, and at the same time, the device is equipped with a high-precision temperature control system inside, which can automatically adjust the storage temperature according to the requirements of different antibodies, thereby maintaining the best active state of the antibodies.

[0024] It also includes a support frame 2, which is slidably embedded in the freezing chamber. The support frame 2 includes a box body formed by a bottom wall and four side walls and opened at one end. The open end of the box body faces the left side of the freezing box 1. Several horizontal plates 7 and vertical plates 8 are fixedly provided inside the support frame 2 to divide the internal space of the support frame 2 into several support chambers for placing reagent racks 21. The freezing box 1 is provided with a driving assembly for driving the support frame 2 to move. A rack 12 is fixedly provided on one side of the support frame 2 along the front-to-back direction of the freezing box 1. The driving assembly includes a motor, which is fixedly installed on the inner wall of the freezing chamber, and a gear 11 meshing with the rack 12 is fixed at the output end of the motor. With such a structural design, the motor drives the gear 11 to rotate, and the gear 11 drives the rack 12 to move, so that the support frame 2 can automatically slide out of the freezing chamber and automatically take or store the corresponding antibodies when needed.

[0025] A lower cavity is provided inside the freezer box 1 below the freezing cavity, and a partition is vertically provided on the inner wall of the lower cavity, which divides the lower cavity into an inner moisturizing cavity 4 and an outer installation cavity 6. A moisturizing layer 5 is provided in the moisturizing cavity 4, and the moisturizing layer 5 is used to maintain the humidity in the freezing cavity. Specifically, the moisturizing layer 5 is a water storage layer or a water-absorbing sponge.

[0026] The drive assembly is installed in the installation cavity 6. A mounting groove 14 is provided at the middle position of the bottom of the support frame 2 along the front-to-back direction of the freezer 1. The rack 12 is fixed in the mounting groove 14. The motor is fixed to the inner wall of the installation cavity 6 by bolts. A connecting groove 13 is provided on the top wall of the installation cavity 6. The installation cavity 6 and the freezer cavity are connected through the connecting groove 13. The top of the gear 11 extends out of the connecting groove 13 and meshes with the rack 12 for transmission. With such a structural design, the rack 12 is located in the middle of the support frame 2, so that the support frame 2 can run smoothly when sliding.

[0027] A connecting frame 3 is integrally formed and fixed on the inner end side wall surface of the supporting frame 2, a cavity 9 is provided inside the connecting frame 3 along the vertical direction, the top of the connecting frame 3 is closed, the bottom of the connecting frame 3 is open and faces the moisturizing cavity 4, a through hole 10 is provided on the top wall of the moisturizing cavity 4, the moisturizing cavity 4 and the cavity 9 are connected via the through hole 10, a first through hole 17 is provided on the side wall of the supporting frame 2 at the inner end and each vertical plate 8 inside the supporting frame 2, a second through hole 18 is provided on the side wall of the supporting frame 2 corresponding to the first through hole 17, the supporting cavity and the cavity 9 are connected via the first through hole 17 and the second through hole 18. With such a structural design, the water vapor in the moisturizing layer 5 can diffuse into each supporting cavity through the through hole 10, the cavity 9, the first perforation 17, and the second perforation 18, so as to moisturize all the flow antibodies. Due to the existence of the moisturizing layer 5, the humidity inside the flow antibody support frame 2 is maintained within a certain range. At this time, even if the sealing performance of the reagent tube 20 is not good, the water in the flow antibody will not evaporate, thereby ensuring that the antibody amount, concentration and antibody activity in the flow antibody do not change.

[0028] It should be noted that the moisturizing layer 5 of the device is not directly connected to the support cavity of the support frame 2. Even if the moisturizing layer 5 is a water storage layer, the movement of the support frame 2 will not cause the liquid in the water storage layer to flow into the support cavity from the through hole 10 and cause liquid contamination.

[0029] Dovetail grooves 15 are respectively provided on both sides of the top end and the bottom end of the support frame 2, and the dovetail grooves 15 extend toward the connecting frame 3 and penetrate the connecting frame 3. A dovetail block 16 matching the dovetail grooves 15 is fixedly provided on the inner wall of the freezing chamber. The dovetail grooves 15 and the dovetail blocks 16 slide in cooperation with each other. Such a structural design enables the support frame 2 to run smoothly. At the same time, when the support frame 2 extends out of the freezing chamber, the connecting frame 3 is still located in the freezing chamber. At this time, the connecting frame 3 can form a stable support for the support frame 2.

[0030] Working principle:

[0031] The operator first inserts the reagent tube 20 containing the flow antibody into the storage hole on the reagent rack 21, then opens the sealed door, starts the motor through the control panel 19, and the motor drives the gear 11 to rotate, and the gear 11 drives the rack 12 and the support frame 2 to slide horizontally in the freezing chamber until the support frame 2 slides out of the freezing chamber. At this time, the connecting frame 3 is still located in the freezing chamber to form a stable support for the support frame 2. The operator can directly place the reagent rack 21 in the support cavity of the support frame 2, and start the motor again, so that the support frame 2 slides into the freezing chamber. The support frame 2 itself seals the corresponding freezing chamber. At this time, the moisturizing cavity 4 is connected to the cavity 9, and the preservation of the flow antibody is completed. During the preservation process of the flow antibody, due to the presence of the moisturizing layer 5, the humidity inside the flow antibody storage box is maintained within a certain range, ensuring that the antibody amount, concentration and antibody activity in the flow antibody do not change.

[0032] The intelligent management device for flow antibodies provided by the utility model can automatically control the storage conditions of antibodies, such as temperature and humidity control, and automatically take out the corresponding antibodies when needed. This greatly reduces the need for manual operation and reduces the risk of antibody activity decline or contamination due to improper operation. The equipment is equipped with a high-precision temperature control system that can automatically adjust the storage temperature according to the requirements of different antibodies, thereby maintaining the optimal activity state of the antibodies. Through automated management, operators can save a lot of time for manual management of antibodies, allowing researchers to focus more on experiments and data analysis themselves. Since the possibility of manual operation errors and antibody degradation is reduced, the use of intelligent management equipment can significantly improve the consistency and reliability of experimental data. At the same time, optimized storage conditions and reduced waste can effectively extend the use cycle of high-priced antibodies, thereby reducing overall costs.

[0033] The above embodiments are only used to illustrate the technical solution of the utility model and are not intended to limit it. Although the utility model is described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model. The technology, shape, and structure that are not described in detail in the utility model are all well-known technologies.

Claims

1. A flow cytometry antibody management device, comprising a freezer (1), wherein a freezing chamber is provided inside the freezer (1), and a sealed door is openably connected to the front end of the freezer (1), characterized in that: The freezer (1) further comprises a support frame (2), wherein the support frame (2) is slidably embedded in the freezing chamber, the support frame (2) comprises a box body with one end open and formed by a bottom wall and four side walls, the open end of the box body facing the left side or the right side of the freezing chamber (1), a plurality of horizontal plates (7) and vertical plates (8) are fixedly provided inside the support frame (2) to divide the internal space of the support frame (2) into a plurality of support chambers, the freezing chamber (1) is provided with a driving assembly for driving the support frame (2) to move, a rack (12) is fixedly provided on one side of the support frame (2) along the front-rear direction of the freezing chamber (1), the driving assembly comprises a motor, the motor is fixedly mounted on the inner wall of the freezing chamber, and a gear (11) meshing with the rack (12) is fixedly provided at the output end of the motor.

2. A flow cytometry antibody management device according to claim 1, characterized in that: The freezer box (1) is provided with a lower cavity below the freezing cavity, and a partition is vertically provided on the inner wall of the lower cavity, and the partition divides the lower cavity into an inner moisturizing cavity (4) and an outer installation cavity (6), a moisturizing layer (5) is provided in the moisturizing cavity (4), and the drive assembly is installed in the installation cavity (6).

3. A flow cytometry antibody management device according to claim 2, characterized in that: A connection frame (3) is fixed on the inner end surface of the support frame (2), a cavity (9) is provided inside the connection frame (3), the top end of the connection frame (3) is closed, the bottom end is open and faces the moisturizing cavity (4), a through hole (10) is provided on the top wall of the moisturizing cavity (4), the moisturizing cavity (4) and the cavity (9) are connected via the through hole (10), a first through hole (17) is provided on the side wall of the support frame (2) at the inner end and each vertical plate (8) inside the support frame (2), a second through hole (18) is provided on the side wall of the support frame (2) at a position corresponding to the first through hole (17), and the support cavity and the cavity (9) are connected via the first through hole (17) and the second through hole (18).

4. A flow cytometry antibody management device according to claim 2, characterized in that: A mounting groove (14) is provided at the middle position of the bottom of the support frame (2) along the front-rear direction of the freezer (1); the rack (12) is fixed in the mounting groove (14); the motor is fixed to the inner wall of the mounting cavity (6); a connecting groove (13) is provided on the top wall of the mounting cavity (6); the mounting cavity (6) and the freezer cavity are connected via the connecting groove (13); the top end of the gear (11) extends out of the connecting groove (13) and meshes with the rack (12) for transmission.

5. A flow cytometry antibody management device according to claim 1, characterized in that: The top and bottom ends of the support frame (2) are respectively provided with dovetail grooves (15), and the inner wall of the freezing chamber is provided with dovetail blocks (16) matching the dovetail grooves (15).

6. A flow cytometry antibody management device according to claim 1, characterized in that: There are a plurality of freezing chambers, which are distributed on the freezing box (1) in a linear array. The freezing box (1) is provided with a temperature control system for controlling and adjusting the temperature of each freezing chamber.

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