Purification device based on bovine serum

Abstract

The bovine serum-based purification device comprises a salting-out cabin, a cabin cover is arranged at the top of the salting-out cabin, and a stirring mechanism is arranged in the middle of the bottom end of the cabin cover; the stirring mechanism comprises a first motor which is located in the middle of the top end of the hatch cover. By arranging the stirring mechanism, when the bovine serum is subjected to salting-out purification, the bovine serum is stirred by the stirring filter rod, and the bovine serum or blocky bovine serum is separated by the filter holes in the stirring filter rod in the stirring process, so that the contact area of the bovine serum and saline water is increased, and the mixing efficiency of the bovine serum and the saline water is improved; a rotating adjusting push plate drives a stirring filter rod to move and be clamped on the inner wall of the salting-out cabin, and an upper clamping plate drives the stirring filter rod to rotate through a positioning plate and the adjusting push plate so as to scrape and brush stains on the inner wall of the salting-out cabin, so that the coarse purification frequency is conveniently reduced, and the coarse purification efficiency is improved; and the inner wall of the device can be conveniently scraped and cleaned after the device is used.

Description

Technical Field

[0001] The utility model relates to the technical field of bovine serum purification, in particular to a purification device based on bovine serum. Background Art

[0002] Bovine serum albumin (BSA), also known as the fifth component, is a globulin in bovine serum. It contains 583 amino acid residues, has a molecular weight of 66.430 kDa, and an isoelectric point of 4.7. As a functional factor, bovine serum albumin is widely used in animal cell culture, diagnostic reagents, food emulsification, etc. It can increase the number of human T lymphocytes and antioxidant activity. Bovine serum purification equipment is divided into centrifuge purification equipment, salting out purification equipment, etc.

[0003] Most of the existing salt precipitation purification equipment consists of a sedimentation chamber, a chamber cover, a stirring rod, a feeding port, a circulation conveying pipe and a drainage pipe.

[0004] The stirring rods in the existing salting-out purification equipment are mostly simple in structure and only have the function of stirring the bovine serum poured into the sedimentation chamber. In addition, most of the existing salting-out purification equipment are not equipped with a filtering mechanism. After purification, the purified bovine serum needs to be subjected to salting-out purification for multiple times, which makes the purification process more complicated. Most of the existing salting-out purification equipment are not equipped with a cleaning mechanism. After the device is used, it is more troublesome to clean the sedimentation chamber. Utility Model Content

[0005] The purpose of the utility model is to provide a purification device based on bovine serum, so as to solve the problem that the stirring rods in the existing salting-out purification equipment proposed in the above-mentioned background technology are mostly simple in structure and only have the function of stirring the bovine serum poured into the sedimentation chamber, and most of the existing salting-out purification equipment are not provided with a filtering mechanism, and the purified bovine serum needs to be subjected to salting-out purification for multiple times after purification, which makes the purification process more complicated, and most of the existing salting-out purification equipment are not provided with a cleaning mechanism, and the subsequent cleaning of the sedimentation chamber after the use of the device is more troublesome.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a purification device based on bovine serum, comprising a salting-out chamber, a hatch cover is provided on the top of the salting-out chamber, and a stirring mechanism is provided in the middle of the bottom end of the hatch cover;

[0007] The stirring mechanism includes a first motor, which is located in the middle of the top end of the hatch cover, and the output end of the first motor is fixedly connected to a driving rotating rod, an upper clamping plate is clamped at the bottom of the driving rotating rod, a mounting column is fixed at the middle of the bottom end of the upper clamping plate, and three positioning plates are evenly distributed on the outside of the mounting column, one end of the positioning plate is rotatably connected to an adjusting push plate, one end of the top of the adjusting push plate is fixedly connected to a connecting gear, a toothed belt is meshed on the outside of the connecting gear, the other end of the adjusting push plate is fixedly connected to a stirring filter rod, and a lower clamping plate is fixedly connected to the bottom of the mounting column.

[0008] Preferably, a plurality of test tube clamp cavities are distributed below the lower clamp plate, and the upper portion of the test tube clamp cavities passes through the upper clamp plate and is fixedly connected to the top of the upper clamp plate.

[0009] Preferably, three positioning hangers are evenly distributed on the outer side of the lower clamping plate, and the positioning hangers are fixedly connected to the outer side of the lower clamping plate, and the positioning hangers are staggered with the positioning plates.

[0010] Preferably, one end below the positioning hanger plate is slidably connected to a support hoop ring plate, and the outer side of the support hoop ring plate is fixedly connected to the inner wall of the top of the salting out tank.

[0011] Preferably, a docking slot is provided at the connection between the driving rotating rod and the middle part of the upper clamping plate, and arc-shaped grooves are evenly distributed on the inner wall of the docking slot. An arc-shaped protrusion is clamped on the inner side of the arc-shaped groove, and the top of the arc-shaped protrusion is fixedly connected to the bottom end of the driving rotating rod.

[0012] Preferably, a semicircular groove matching the stirring filter rod is formed on the outer surface of the lower clamping plate, and the semicircular groove is adjacent to the positioning hanging plate.

[0013] Preferably, a second motor is provided at one end of the top of the upper clamping plate, and an output end of the second motor is fixedly connected to the middle part of the top end of the connecting gear.

[0014] Compared with the prior art, the utility model has the following beneficial effects: it is convenient to perform preliminary filtration on the purified bovine serum when the bovine serum is roughly purified by salting out, it is convenient to reduce the number of rough purifications, which is beneficial to improve the efficiency of rough purification, and it is convenient to scrape and clean the inner wall of the device after the device is used.

[0015] Through the stirring mechanism, when the bovine serum is subjected to salting out and purification, the bovine serum is stirred by the stirring filter rod. During the stirring process, the filter holes on the stirring filter rod separate the bovine serum or the agglomerated bovine serum, thereby increasing the contact area between the bovine serum and the brine and improving the mixing efficiency of the bovine serum and the brine. During cleaning, the stirring filter rod is driven by the rotating adjusting push plate to move and clamp onto the inner wall of the salting out chamber, and then the upper clamping plate drives the stirring filter rod to rotate through the positioning plate and the adjusting push plate to scrape and brush the stains on the inner wall of the salting out chamber, thereby facilitating the preliminary filtration of the purified bovine serum when the bovine serum is subjected to salting out and crude purification, thereby reducing the number of crude purification times and improving the efficiency of crude purification, and facilitating the scraping and brushing of the inner wall of the device after the device is used. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the cross-sectional structure of the main view of the utility model;

[0017] Figure 2 This is a structural schematic diagram of a top view of the connection between the lower clamping plate and the adjusting push plate of the utility model;

[0018] Figure 3 For this utility model Figure 1 The structural diagram of the enlarged view of the middle A part;

[0019] Figure 4 It is a structural schematic diagram of a disassembled stereoscopic diagram of the stirring filter rod and the adjusting push plate of the utility model.

[0020] In the figure: 1, salt precipitation chamber; 2, hatch cover; 3, stirring mechanism; 31, stirring filter rod; 32, lower clamping plate; 33, upper clamping plate; 34, first motor; 35, mounting column; 36, positioning plate; 37, adjusting push plate; 38, connecting gear; 39, toothed belt; 310, test tube clamp cavity; 311, docking slot; 312, driving rotating rod; 313, second motor; 4, support hoop ring plate; 5, positioning hanging plate. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] See also Figure 1 , Figure 2 , Figure 3 and Figure 4The utility model provides a technical solution: a purification device based on bovine serum, comprising a salting-out chamber 1, a hatch cover 2 is provided on the top of the salting-out chamber 1 for sealing the top of the salting-out chamber 1, and a stirring mechanism 3 is provided in the middle of the bottom end of the hatch cover 2;

[0023] The stirring mechanism 3 includes a first motor 34, which is located in the middle of the top of the hatch cover 2 and is used to drive the driving rotating rod 312 to rotate. The output end of the first motor 34 is fixedly connected to the driving rotating rod 312, which is used to drive the upper clamping plate 33 to rotate. The upper clamping plate 33 is clamped below the driving rotating rod 312 to block the upward flowing hot air flow. A mounting column 35 is fixed to the middle of the bottom end of the upper clamping plate 33, which is used to fix the positioning plate 36 and to connect the upper clamping plate 33 and the lower clamping plate 32. Three positioning plates 36 are evenly distributed on the outside of the mounting column 35 for positioning the adjusting push plate 37. One end of the positioning plate 36 is rotatably connected to the adjusting push plate 37 for adjusting the position of the stirring filter rod 31. One end of the top of the adjusting push plate 37 is fixedly connected to a connecting gear 38 for driving the adjusting push plate 37 fixedly connected at the bottom to rotate. A toothed belt 39 is meshed on the outside of the connecting gear 38 for driving the three connecting gears The bottom of the adjusting column 35 is fixedly connected with a lower clamping plate 32 for supporting the bottom of the adjusting push plate 37. A plurality of test tube clamp cavities 310 are distributed below the lower clamping plate 32 for clamping sample test tubes. The upper part of the test tube clamp cavity 310 passes through the upper clamping plate 33 and is fixedly connected to the top of the upper clamping plate 33 for fixing the test tube clamp cavity 310. The outer surface of the lower clamping plate 32 is provided with a semicircular groove matching the stirring filter rod 31 for clamping the top of the folded stirring filter rod 31. The semicircular groove is adjacent to the positioning hanging plate 5. A second motor 313 is provided at one end of the top of the upper clamping plate 33. The output end of the second motor 313 is fixedly connected to the middle part of the top of the connecting gear 38 for driving the connecting gear 38 to rotate.

[0024] See also Figure 2 and Figure 3 A docking slot 311 is provided at the connection between the driving rotating rod 312 and the middle part of the upper clamping plate 33. Arc-shaped grooves are evenly distributed on the inner wall of the docking slot 311. An arc-shaped protrusion is clamped on the inner side of the arc-shaped groove. The top of the arc-shaped protrusion is fixedly connected to the bottom end of the driving rotating rod 312, which is used to fix the driving rotating rod 312 clamp to the upper clamping plate 33.

[0025] See also Figure 1 and Figure 2Three positioning hangers 5 are evenly distributed on the outer side of the lower splint 32, and the positioning hangers 5 are fixedly connected to the outer side of the lower splint 32 for suspending the lower splint 32. The positioning hangers 5 are staggered with the positioning plates 36. A support hoop plate 4 is slidably connected to one end below the positioning hangers 5. The outer side of the support hoop plate 4 is fixedly connected to the inner wall of the top of the salt precipitation tank 1 for supporting and positioning the positioning hangers 5.

[0026] Working principle: during use, when a large amount of bovine serum needs to be salted out and purified, the bovine serum is first injected into the stirring filter rod 31 through the opening at the top of the stirring filter rod 31. After entering the stirring filter rod 31, the bovine serum flows into the salting-out chamber 1 through the filter holes on the outside of the stirring filter rod 31. When the bovine serum passes through the filter holes and enters the salting-out chamber 1, the preliminary filtration of the bovine serum is completed, and then the second motor 313 is started, and the second motor 313 drives the corresponding connecting gear 38 at the bottom to rotate. The rotating connecting gear 38 will drive the other two connecting gears 38 to rotate synchronously through the toothed belt meshing on the outside, and the rotating connecting gear 38 will drive the corresponding adjusting push plate 37 at the bottom to rotate inward or outward by a certain angle, thereby pushing the stirring filter rod 31 fixedly connected at one end of the adjusting push plate 37 to move a specified distance in a direction away from the inner middle of the salting-out chamber 1 or in a direction close to the inner middle of the salting-out chamber 1, and then the power supply of the second motor 313 is disconnected;

[0027] The power supply of the first motor 34 is started, and the driving rotating rod 312 fixedly connected to the output end of the first motor 34 drives the upper clamping plate 33 clamped at the bottom end of the driving rotating rod 312 to rotate. The rotating upper clamping plate 33 will drive the stirring filter rod 31 fixedly connected to one end of the adjusting push plate 37 to rotate through the mounting column rod 35 fixedly connected to the middle part of the bottom end and the positioning plate 36 and the adjusting push plate 37 provided on the outer side of the mounting column rod 35. The rotating stirring filter rod 31 will stir the bovine serum in the salting-out chamber 1 to mix it with the purified brine. The filter holes on the stirring filter rod 31 will rotate under the drive of the stirring filter rod 31 to filter and crush the bovine serum, prevent the bovine serum from adhering to blocks, and increase the contact area between the brine and the bovine serum, thereby improving the mixing effect of the brine and the bovine serum. During purification, the sample test tube is clamped into the test tube clamp cavity 310 to fix the sample test tube, and then the rotating upper clamp plate 33 drives the clamped test tube to produce centrifugal rotation, so that the bovine serum sample in the test tube produces liquid separation. When the inner wall of the salting-out chamber 1 needs to be cleaned, the adjusting push plate 37 drives the stirring filter rod 31 to move in a direction away from the inner middle part of the salting-out chamber 1. When the stirring filter rod 31 is clamped on the inner wall of the salting-out chamber 1, the adjusting push plate 37 is stopped from rotating. Then the rotating upper clamp plate 33 drives the stirring filter rod 31 to rotate along the inner wall of the salting-out chamber 1 through the positioning plate 36, and the striped blocks on the stirring filter rod 31 are used to scrape and clean the stains attached to the inner wall of the salting-out chamber 1. The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0028] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A purification device based on bovine serum, comprising a salting-out chamber (1), characterized in that: The top of the salt precipitation chamber (1) is provided with a hatch cover (2), and the middle part of the bottom end of the hatch cover (2) is provided with a stirring mechanism (3); The stirring mechanism (3) comprises a first motor (34), wherein the first motor (34) is located in the middle of the top end of the hatch cover (2), the output end of the first motor (34) is fixedly connected to a driving rotating rod (312), an upper clamping plate (33) is clamped below the driving rotating rod (312), a mounting column (35) is fixed in the middle of the bottom end of the upper clamping plate (33), three positioning plates (36) are evenly distributed on the outside of the mounting column (35), one end of the positioning plate (36) is rotatably connected to an adjusting push plate (37), one end of the top of the adjusting push plate (37) is fixedly connected to a connecting gear (38), a toothed belt (39) is meshed on the outside of the connecting gear (38), the other end of the adjusting push plate (37) is fixedly connected to a stirring filter rod (31), and a lower clamping plate (32) is fixedly connected below the mounting column (35).

2. A purification device based on bovine serum according to claim 1, characterized in that: A plurality of test tube clamp cavities (310) are distributed below the lower clamp plate (32), and the upper portion of the test tube clamp cavities (310) passes through the upper clamp plate (33) and is fixedly connected to the top of the upper clamp plate (33).

3. A purification device based on bovine serum according to claim 1, characterized in that: Three positioning hangers (5) are evenly distributed on the outer side of the lower clamping plate (32), and the positioning hangers (5) are fixedly connected to the outer side of the lower clamping plate (32), and the positioning hangers (5) and the positioning plates (36) are staggered.

4. A purification device based on bovine serum according to claim 3, characterized in that: One end below the positioning hanging plate (5) is slidably connected to a support hoop ring plate (4), and the outer side of the support hoop ring plate (4) is fixedly connected to the inner wall of the top of the salt precipitation tank (1).

5. The bovine serum-based purification device according to claim 1, characterized in that: A docking slot (311) is provided at the connection between the driving rotating rod (312) and the middle part of the upper clamping plate (33), and arc-shaped grooves are evenly distributed on the inner wall of the docking slot (311). An arc-shaped protrusion is clamped on the inner side of the arc-shaped groove, and the top of the arc-shaped protrusion is fixedly connected to the bottom end of the driving rotating rod (312).

6. A purification device based on bovine serum according to claim 1, characterized in that: The outer surface of the lower clamping plate (32) is provided with a semicircular groove matching the stirring filter rod (31), and the semicircular groove is adjacent to the positioning hanging plate (5).

7. A purification device based on bovine serum according to claim 1, characterized in that: A second motor (313) is provided at one end of the top of the upper clamping plate (33), and an output end of the second motor (313) is fixedly connected to the middle part of the top end of the connecting gear (38).

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