Micro sperm freezing capturer

Abstract

The micro sperm freezing capturer comprises a micro-filter screen head, a hollow connecting rod, a protective sleeve head and an injection pump, the injection pump can be arranged in an injection pump fixing frame, one end of the hollow connecting rod is connected with the injection pump, and the other end of the hollow connecting rod is connected with the micro-filter screen head; a protective sleeve head is arranged on the outer layer of the micro-filter screen head, a liquid inlet and a liquid outlet are formed in the protective sleeve head, and the liquid inlet and the liquid outlet are sealed through a sealing cap. According to the utility model, rapid collection, rapid cleaning, rapid freezing, rapid unfreezing and rapid collection after unfreezing of trace cells, gametes or embryos can be integrated, and damage to the trace cells, gametes or embryos during collection, cleaning, freezing, unfreezing recovery and collection after recovery is reduced. As a novel micro-cell, gamete or embryo collecting and freezing device, the micro-cell, gamete or embryo collecting and freezing device has a wide application prospect in the aspects of assisted reproduction and biological breeding.

Description

Technical Field

[0001] The utility model belongs to the technical field of medical assisted reproductive devices, and particularly relates to a micro sperm freezing capture device. Background Art

[0002] In recent years, there are more and more patients with severe oligoasthenozoospermia and azoospermia. Because the number of sperm obtained from patients with severe oligoasthenozoospermia and azoospermia (some patients can obtain sperm through epididymal / testicular puncture or microsurgery) is small, the sperm obtained is very precious, and biological offspring can only be obtained through assisted reproductive technology. In the process of assisted reproductive technology, these patients often have excessive mental stress and low spermatogenesis, which will cause their spermatogenesis to decline further. If sperm is collected on the day of egg retrieval, they often face the embarrassing situation of not being able to obtain sperm. Clinically, the freezing technology of eggs is not very mature, and the recovery rate after freezing is low. Therefore, before preparing for assisted reproductive technology treatment, the best treatment plan is to collect sperm in advance and select high-quality sperm for freezing. The collection, freezing, and recovery effect of trace sperm, as well as the timely capture of trace high-quality sperm in the recovery fluid after recovery are the keys to the success of assisted reproductive technology treatment.

[0003] The existing micro sperm freezing devices mainly include: (1) one uses human zona pellucida as a freezing carrier, but the source of human zona pellucida and the experimental equipment and technology required for its preparation limit its clinical application. (2) the other uses ordinary straws, modified straws, dry ice or frozen stainless steel plates, freezing rings, microinjection needles, Cell Sleepers, freezing blades, and glass capillaries. These freezing devices have the following problems: first, these freezing devices are difficult to collect and obtain after freezing and thawing. It is difficult to find a small amount of high-quality sperm in the cleaning solution or thawing solution in a short time (high-quality sperm swims fast). Second, these freezing devices (except glass capillaries) are open freezing, lack of carrier isolation, and cannot avoid potential cross-infection. Third, these freezing devices can cause sperm loss due to the adsorption effect of the wall. Cell Sleepers are relatively better and reduce sperm loss. Fourth, glass capillaries also have the problem of being fragile when frozen at ultra-low temperatures. Therefore, the current micro sperm freezing devices are not ideal sperm freezing devices.

[0004] In summary, there is an urgent need for a micro-sperm freezing and capturing device that is simple to operate, can quickly collect, quickly freeze, quickly thaw, and can quickly obtain sperm after thaw; can avoid the possibility of contamination, and has high biological safety. Summary of the invention

[0005] The utility model aims to provide a device for quickly collecting, freezing and thawing a small amount of sperm, and quickly capturing the sperm after thawing, in view of the shortcomings of the existing micro sperm freezing devices.

[0006] The technical solution of the utility model is as follows: a micro-sperm freezing capture device comprises a microfiltration mesh head, a hollow connecting rod, a protective sleeve head and an injection pump, wherein the injection pump is placed in an injection pump fixing frame, one end of the hollow connecting rod is connected to the injection pump, and the other end is connected to the microfiltration mesh head; a protective sleeve head is arranged on the outer layer of the microfiltration mesh head, and a liquid inlet and outlet are arranged on the protective sleeve head, and the liquid inlet and outlet are closed by a sealing cap.

[0007] The microfilter head is made of super-hydrophobic biomaterial, and the mesh size of frozen human sperm is about 1.5um (the mesh size of frozen human eggs can be about 150um; the mesh size of frozen human embryos can be about 200um; the appropriate mesh size can be selected according to the frozen cells, gametes or embryos). The microfilter head is designed to be elliptical and streamlined, considering that during the flushing process, the cells, gametes or embryos are in full contact with the liquid to prevent the cells, gametes or embryos from adhering to the filter wall. The material of the microfilter head is super-hydrophobic biomaterial to prevent liquid, cells, gametes or embryos from adsorbing to the microfilter head. The microfilter head is connected to a hollow connecting rod, and the microfilter head is covered with a protective sleeve.

[0008] The hollow connecting rod is made of biocompatible superhydrophobic material, one end of which is connected to the microfiltration head, and the other end of which can be connected to the injection pump; (when not connected, the opening of the hollow connecting rod can be closed with a sealing cap) If necessary, it can be made into a hollow connecting rod that can be fixed on a fixed bracket.

[0009] The protective sleeve head is made of biocompatible hydrophobic material and is used for the protective sleeve head of the microfiltration mesh head. The interface is clamped in the connecting part between the hollow connecting rod and the microfiltration mesh head. A gap is reserved between the protective sleeve head and the microfiltration mesh head. The protective sleeve head is designed to use male and female surfaces that are buckled with male and female buckles; the male and female surfaces of the protective sleeve head each have a raised opening, one is a liquid inlet and the other is a liquid outlet; the raised openings of the protective sleeve head can be closed with a sealing cap.

[0010] The hollow connecting hose and the sealing cap are both made of polymer composite biocompatible materials. The hollow connecting hose is connected to the raised opening of the protective sleeve, the liquid inlet is connected to the injection pump, and the liquid outlet is connected to the waste liquid bag. The sealing cap is used to close the raised opening of the protective sleeve and the opening of the hollow connecting rod.

[0011] Operation steps:

[0012] Sperm collection and cleaning: the injection pump draws semen, flows through the hollow connecting rod, is injected into the microfilter head, and is pressurized to filter the semen; the injection pump draws cleaning fluid, flows through the hollow connecting rod, is injected into the microfilter head, and is pressurized to clean the sperm.

[0013] Sperm freezing: The cleaned sperm is retained in the microfilter. After being quickly filled with liquid nitrogen through the hollow connecting rod, the protective cover is put on. The inlet and outlet of the protective cover and the opening of the hollow connecting rod are sealed with sealing caps. After being labeled, the sperm is stored in a liquid nitrogen tank.

[0014] Sperm resuscitation: Take out the frozen sperm from the liquid nitrogen tank, remove the sealing caps of the inlet and outlet ports of the protective sleeve and the opening of the hollow connecting rod, connect the injection pump to the opening of the hollow connecting rod, blow away the liquid nitrogen, and quickly and cyclically inject the resuscitation liquid through the inlet and outlet ports of the protective sleeve and the opening of the hollow connecting rod. After the sperm is quickly resuscitated, blow away the resuscitation liquid through the hollow connecting rod.

[0015] Collection of revived sperm: Remove the protective sleeve and absorb the culture medium through the microfilter. The culture medium and sperm are sucked into the injection pump through the hollow connecting rod for use.

[0016] The utility model has the following beneficial effects:

[0017] (1) The utility model is provided with a microfilter head, a hollow rod connected to the microfilter head, and a protective sleeve of the microfilter head. It can realize the rapid collection, rapid cleaning, rapid freezing, rapid thawing, and rapid collection after thawing of trace cells, gametes, or embryos, thereby reducing the damage of trace cells, gametes, or embryos during the collection, cleaning, freezing, thawing and recovery, and collection after recovery. As a new device for collecting and freezing trace cells, gametes, or embryos, it has broad application prospects in human assisted reproduction and biological breeding, and is worthy of further promotion and application.

[0018] (2) The mesh of the microfiltration head of the utility model can be designed with different pore sizes according to the size of frozen cells, gametes or embryos, which is conducive to the collection and processing of cells, gametes or embryos.

[0019] (3) The protective cover of the microfilter head of the utility model can be used as a container for cleaning solution, freezing solution and resuscitation solution during the process of collecting, cleaning, freezing and resuscitation of cells, gametes or embryos, which is conducive to the rapid cleaning, freezing and resuscitation of cells, gametes or embryos; it can also be used as a protective cover to avoid contamination.

[0020] (4) The utility model can realize the collection, cleaning, freezing, cryopreservation and resuscitation of trace cells, gametes or embryos in one device, thus saving consumables.

[0021] (5) The various components of the utility model can be disassembled and combined as needed. After assembly, the operation is simple and can be operated by one person, thus saving human resources.

[0022] (6) The microfiltration head of the utility model is designed to be streamlined and the material used is selected to be highly hydrophobic and biocompatible material, which can achieve the effects of rapid collection, cleaning, freezing and resuscitation while avoiding the loss and damage of cell, gamete or embryo adhesion.

[0023] (7) The entire device of the utility model can be designed to be transparent, which is convenient for operation and observation. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the structure of the utility model;

[0025] Figure 2 This is a schematic diagram of the structure of the micro-filter head and the hollow connecting rod of the utility model;

[0026] Figure 3 It is a structural schematic diagram of the micro-filter head protective cover of the utility model.

[0027] Figure 1-Figure 3 Middle: 1. Microfilter head; 2. Hollow connecting rod; 3. Protective sleeve head; 4. Sealing cap; 5. Liquid inlet and outlet; 6. Syringe pump; 7. Syringe pump fixing bracket. DETAILED DESCRIPTION

[0028] The specific implementation of the utility model is described in conjunction with the accompanying drawings.

[0029] like Figure 1-Figure 3 As shown, the micro-sperm freezing capture device includes a microfiltration mesh head 1, a hollow connecting rod 2, a protective sleeve head 3 and an injection pump 6. The injection pump 6 is placed in an injection pump fixing frame 7. One end of the hollow connecting rod 2 is connected to the injection pump 6, and the other end is connected to the microfiltration mesh head 1; the outer layer of the microfiltration mesh head 1 is provided with a protective sleeve head 3, and the protective sleeve head 3 is provided with a liquid inlet and outlet 5, and the liquid inlet and outlet 5 are closed by a sealing cap 4.

[0030] The injection pump 6 can be connected to the raised opening 5 of the protective sleeve head 3 through a hose, the liquid inlet is connected to the injection pump 6, and the liquid outlet is connected to the waste liquid bag; the function of the sealing cap 4 is to close the raised opening 5 of the protective sleeve head 3 and the opening of the hollow connecting rod 2.

[0031] As a preferred technical solution, the mesh of the microfiltration head 1 can be designed with a pore size according to the size of the frozen cells, gametes or embryos.

[0032] As a preferred technical solution, the protective cover head 3 is designed as a parent-child buckle type, which is convenient for disassembly as needed.

[0033] As a preferred technical solution, each component can be disassembled and combined as needed. After assembly, the operation is simple and can be performed by one person, thus saving human resources and reducing damage to cells, gametes or embryos during freezing and thawing.

[0034] As a preferred technical solution, the utility model can be used in the freezing and recovery process of cells, gametes or embryos to achieve rapid collection, rapid cleaning, rapid freezing, rapid thawing, rapid collection after thawing, and can be directly used as a carrier tool for cryopreservation, saving consumables costs.

[0035] As a preferred technical solution, the liquid inlet of the protective sleeve 3 can be directly designed as an opening interface of the injection pump (device), reducing connecting hoses and consumables; the liquid outlet of the protective sleeve can be directly connected to a waste liquid bag or a waste liquid barrel.

[0036] As a preferred technical solution, the micro-filter head 1, the protective sleeve head 3, and the hollow connecting rod 2 can all be made transparent, which is conducive to operation and observation.

[0037] As a preferred technical solution, one side of the microfiltration head 1 is designed to be flat, which is convenient for operation in a plate.

Claims

1. A micro sperm freezing capture device, characterized in that: It includes a microfiltration mesh head, a hollow connecting rod, a protective sleeve head and an injection pump. The injection pump can be placed in an injection pump fixing frame. One end of the hollow connecting rod is connected to the injection pump, and the other end is connected to the microfiltration mesh head. A protective sleeve head is arranged outside the microfiltration mesh head, and a liquid inlet and outlet are arranged on the protective sleeve head. The liquid inlet and outlet are closed by sealing caps.

2. The micro-sperm freezing trap according to claim 1, characterized in that: The microfiltration mesh head is made of super hydrophobic biological material.

3. The micro sperm freezing trap according to claim 1, characterized in that: The protective sleeve head is made of a biocompatible hydrophobic material and is used for the protective sleeve head of the microfiltration mesh head. The interface is clamped at the connection part between the hollow connecting rod and the microfiltration mesh head. A gap is reserved between the protective sleeve head and the microfiltration mesh head. The protective sleeve head is designed as a sub-surface that is buckled with a sub-surface buckle. The sub-surfaces of the protective sleeve head each have a raised opening, one for the liquid inlet and the other for the liquid outlet. The raised opening of the protective sleeve head can be closed with a sealing cap.

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