Coomassie brilliant blue dyeing device

Abstract

The utility model provides a coomassie brilliant blue dyeing device which comprises a dyeing box, a box door is rotatably installed at the front end of the dyeing box through a hinge, a soaking box is placed at the bottom of the dyeing box, four sliding rods are fixed to the four corners of the soaking box and the inner wall of the top of the dyeing box, and placing assemblies are installed on the surfaces of the sliding rods in a sliding mode. A partition assembly is arranged at the top of the placement assembly, the placement assembly comprises a placement frame and a lower frame, and compared with the prior art, the placement frame is installed at the bottom of the partition assembly, the top plate shields the top of the placement frame, and the lower frame shields the bottom and the two sides of the placement frame, so that polyacrylamide gel in the placement frame can be conveniently washed through the top; the waste water is independently collected, and the placing frame can be leaked out through the movement of the lower frame, so that the placing frame is fed into the soaking box, the polyacrylamide gel is conveniently dyed, the polyacrylamide gel does not need to be transferred in the middle process, and the dyeing efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of dyeing devices, in particular to a Coomassie brilliant blue dyeing device. Background Art

[0002] When staining with Coomassie Brilliant Blue stain, the polyacrylamide gel after electrophoresis needs to be rinsed, and the rinsed polyacrylamide gel is placed in a staining dish for staining, and then it needs to be taken out and rinsed. During this process, the polyacrylamide gel needs to be shaken on a decolorizing shaker. These steps are performed separately, which greatly increases the staining time.

[0003] Patent CN212301060U proposes a polyacrylamide gel electrophoresis Coomassie brilliant blue rapid staining device, which places the polyacrylamide gel on a mesh plate and pushes it into a box, rinses the polyacrylamide gel on the mesh plate through a flushing mechanism and then drains it, then places a staining dish filled with a staining solution on a movable support plate through a positioning groove and pushes it into the box, starts a first motor to drive the polyacrylamide gel on the mesh plate to descend to the staining dish through a lifting frame for staining, shakes the polyacrylamide gel in staining through a shaking table seat mechanism, takes out the staining dish after staining is completed, rinses the polyacrylamide gel again through a flushing mechanism, drains it, and then takes it out for observation.

[0004] In the above scheme, the polyacrylamide gel is stained by a mesh plate and a staining dish. However, during the shaking process, because there is no shielding above the staining dish, the Coomassie Brilliant Blue stain is easily spilled on the outside due to shaking, thereby polluting the environment in the box. In addition, it is not convenient to collect and process different solutions for rinsing and soaking separately. Utility Model Content

[0005] In view of the shortcomings of the prior art, the utility model aims to provide a Coomassie Brilliant Blue staining device to solve the problems raised in the above-mentioned background technology. The utility model has a novel structure. A placement frame is installed at the bottom of a partition component, a top plate shields the top of the placement frame, and a lower frame shields the bottom and both sides of the placement frame. The polyacrylamide gel in the placement frame can be easily rinsed through the top, and waste water can be collected separately. The placement frame can be leaked out by moving the lower frame, and then sent into a soaking box, so that the polyacrylamide gel can be easily stained. In the intermediate process, the polyacrylamide gel does not need to be transported, thereby improving the staining efficiency.

[0006] In order to achieve the above-mentioned purpose, the utility model is realized by the following technical scheme: a Coomassie brilliant blue dyeing device comprises a dyeing box, a box door is installed at the front end of the dyeing box through hinge rotation, the bottom of the dyeing box is placed on a soaking box, four sliding rods are fixed at the four corners of the soaking box and the inner wall of the top of the dyeing box, and a placement component is slidably installed on the surface of the sliding rod, a partition component is provided on the top of the placement component, the placement component comprises a placement frame and a lower frame, openings are provided on both sides of the placement frame, and the lower frame slides along both sides of the placement frame, the partition component comprises a top plate, the top plate is slidably sleeved on the sliding rod and is located at the top of the placement frame, an upper frame is slidably installed on the top of the top plate, and the upper frame is transmission-connected to the lower frame.

[0007] Furthermore, the partition assembly also includes a horizontal plate, which is fixed on both sides of the bottom of the top plate and slidably sleeved on the sliding rod. A screw device is arranged inside the horizontal plate, and a sliding frame that slides along the horizontal plate is meshed and sleeved on the outer surface of the screw device.

[0008] Furthermore, the placement component also includes an inserting plate, and the inserting plates are fixed at both ends of the placement frame, and the inserting plates can be snap-fitted and inserted into the interior of the sliding frame.

[0009] Furthermore, slide grooves are provided at the bottoms of both sides of the upper rack, and sliders are slidably connected inside the slide grooves. Inserting posts are slidably inserted in the sliders, and the inserting posts can be inserted into corresponding positions of the lower rack.

[0010] Furthermore, electric push rods are fixed on both sides of the top plate located on the upper frame, and the extended ends of the electric push rods are fixedly connected to the upper frame.

[0011] Furthermore, drain grooves are provided on the surfaces of the top plate and the placement frame.

[0012] Furthermore, a spray rack is installed on the inner wall of the top of the dyeing box, and two rows of spray heads facing the top plate drain grooves are arranged at the bottom of the spray rack.

[0013] Furthermore, a drainage pipe is provided on the outward side surface of the lower frame.

[0014] Beneficial effects of the utility model: A Coomassie brilliant blue dyeing device of the utility model comprises a dyeing box; a box door; a soaking box; a sliding rod; a spray rack; a placement component; a placement frame; a lower rack; a drain pipe; a plug board; a partition component; a top plate; an electric push rod; an upper rack; a slide groove; a slider; a plug column; a horizontal plate; a screw rod device; a slide frame;

[0015] 1. After the placing frame is inserted into the sliding frame through the plug plate, the plug posts are inserted into both sides of the lower frame, and the upper frame is plugged with the lower frame, so that the upper frame and the lower frame can move vertically synchronously. At the same time, when the placing frame moves left and right, the slider slides along the slide groove to keep the connection between the upper frame and the lower frame.

[0016] 2. The utility model uses an obliquely arranged spray rack to rinse the polyacrylamide gel in the placement frame, and sends the waste water out from the drain pipe to prevent it from falling into the soaking box. Similarly, during soaking and dyeing, the upper rack blocks the leakage groove of the top plate, and the lower rack leaks the leakage grooves on both sides and the bottom of the placement frame, making it convenient for the dye to enter the placement frame to dye the polyacrylamide gel.

[0017] 3. Compared with the prior art, the utility model has a placement frame installed at the bottom of the partition assembly, a top plate shielding the top of the placement frame, and a lower frame shielding the bottom and both sides of the placement frame, so that the polyacrylamide gel in the placement frame can be easily rinsed through the top, and the waste water can be collected separately. By moving the lower frame, the placement frame can be leaked out and sent into the immersion box, which is convenient for dyeing the polyacrylamide gel. There is no need to transport the polyacrylamide gel in the intermediate process, thereby improving the dyeing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the overall structure of a Coomassie Brilliant Blue staining device of the utility model;

[0019] Figure 2 This is one of the schematic diagrams of the connection between the partition component and the placement component of a Coomassie Brilliant Blue staining device of the utility model;

[0020] Figure 3 This is the second schematic diagram of the connection between the partition component and the placement component of a Coomassie Brilliant Blue staining device of the utility model;

[0021] Figure 4 This is the third schematic diagram of the connection between the partition component and the placement component of a Coomassie Brilliant Blue staining device of the utility model.

[0022] In the figure: 1. dyeing box; 11. box door; 12. soaking box; 13. sliding rod; 2. spray rack; 3. placement component; 31. placement frame; 32. lower rack; 33. drain pipe; 34. plug board; 4. partition component; 41. top plate; 42. electric push rod; 43. upper rack; 44. slide groove; 45. slider; 46. plug column; 47. cross plate; 48. screw device; 49. sliding frame. DETAILED DESCRIPTION

[0023] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0024] See also Figures 1 to 4The utility model provides a technical solution: a Coomassie brilliant blue staining device, comprising a staining box 1, a box door 11 is installed at the front end of the staining box 1 through a hinge, the bottom of the staining box 1 is placed on a soaking box 12, four sliding rods 13 are fixed at the four corners of the soaking box 12 and the top inner wall of the staining box 1, and a placement component 3 is slidably installed on the surface of the sliding rod 13, a partition component 4 is provided on the top of the placement component 3, the placement component 3 includes a placement frame 31 and a lower frame 32, both sides of the placement frame 31 are provided with openings, and the lower frame 32 slides along both sides of the placement frame 31, and the partition component 4 includes a top The top plate 41 is slidably sleeved on the slide bar 13 and is located at the top of the placement frame 31. The top of the top plate 41 is slidably installed with an upper frame 43, and the upper frame 43 is transmission-connected to the lower frame 32. When the device is used, the polyacrylamide gel is placed inside the placement frame 31, and then installed at the bottom of the partition component 4, and then slid along the slide bar 13 to send the placement frame 31 into the immersion box 12. The top plate 41 blocks the top of the immersion box 12, and then the placement frame 31 moves back and forth horizontally to stain the polyacrylamide gel inside, and then the placement frame 31 is pulled up to rinse the polyacrylamide gel, and the waste water is sent out separately.

[0025] In this embodiment, the partition assembly 4 also includes a transverse plate 47, and the transverse plates 47 are fixed on both sides of the bottom of the top plate 41, and the transverse plates 47 are slidably sleeved on the slide rod 13. A screw device 48 is arranged inside the transverse plate 47, and a slide frame 49 that slides along the transverse plate 47 is meshed and sleeved on the outer surface of the screw device 48. The placement assembly 3 also includes an insert plate 34, and the insert plates 34 are fixed at both ends of the placement frame 31, and the insert plates 34 can be snap-fitted into the slide frame 49. The screw device 48 is a common motor and screw driving method, which drives the slide frame 49 and the placement frame 31 to move horizontally, accelerates the contact between the polyacrylamide gel and the dye for dyeing, and the polyacrylamide gel can be taken out after the placement frame 31 is removed by separating the insert plate 34 from the slide frame 49.

[0026] In this embodiment, a slide groove 44 is provided at the bottom of both sides of the upper shelf 43, and a slider 45 is slidably connected inside the slide groove 44. A plug post 46 is slidably inserted in the slider 45, and the plug post 46 can be inserted into the corresponding position of the lower shelf 32. After the placement frame 31 is inserted into the slide frame 49 through the plug plate 34, the plug post 46 is inserted into both sides of the lower shelf 32, and the upper shelf 43 is plugged with the lower shelf 32, so that the upper shelf 43 and the lower shelf 32 can move vertically synchronously. At the same time, when the placement frame 31 moves left and right, the slider 45 slides along the slide groove 44 to keep the upper shelf 43 connected to the lower shelf 32.

[0027] In this embodiment, the top plate 41 is located on both sides of the upper frame 43 and is fixed with electric push rods 42, and the extended ends of the electric push rods 42 are fixedly connected to the upper frame 43. The top plate 41 and the placement frame 31 are both provided with drain grooves on their surfaces. A spray rack 2 is installed on the top inner wall of the dyeing box 1, and two rows of nozzles facing the drain grooves of the top plate 41 are provided at the bottom of the spray rack 2. A drain pipe 33 is provided on the outward side surface of the lower frame 32. Due to the position setting of the upper frame 43 and the lower frame 32, the drain grooves of the top plate 41 and the placement frame 31 are opened alternately. , the upper rack 43 is moved upward by electric pushing, and the lower rack 32 blocks the bottom and both sides of the placement frame 31. Then, one end of the drain pipe 33 is manually connected to the external hose, and the polyacrylamide gel in the placement frame 31 is flushed through the obliquely arranged spray rack 2, and the waste water is sent out from the drain pipe 33 to avoid falling into the immersion box 12. Similarly, during immersion dyeing, the upper rack 43 blocks the leakage groove of the top plate 41, and the lower rack 32 leaks the leakage grooves on both sides and the bottom of the placement frame 31, which facilitates the dye to enter the placement frame 31 to dye the polyacrylamide gel.

[0028] When using the device, the polyacrylamide gel is placed in the placement frame 31, and then installed at the bottom of the partition component 4, and then slid along the slide rod 13 to send the placement frame 31 into the immersion box 12, and the top plate 41 blocks the top of the immersion box 12. Then the placement frame 31 moves back and forth horizontally to dye the polyacrylamide gel inside, and then the placement frame 31 is pulled up to rinse the polyacrylamide gel, and the waste water is sent out separately. The upper frame 43 is moved up by electric pushing, and the lower frame 32 blocks the bottom and both sides of the placement frame 31. Then, one end of the drain pipe 33 is manually connected to the external hose, and the polyacrylamide gel in the placement frame 31 is rinsed through the obliquely arranged spray rack 2, and the waste water is sent out from the drain pipe 33 to avoid falling into the immersion box 12. Similarly, during immersion dyeing, the upper frame 43 blocks the leakage groove of the top plate 41, and the lower frame 32 leaks the leakage grooves on both sides and the bottom of the placement frame 31, so as to facilitate the dye to enter the placement frame 31 to dye the polyacrylamide gel.

[0029] The basic principles and main features of the present invention and the advantages of the present invention are shown and described above. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention.

[0030] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A Coomassie Brilliant Blue staining device, comprising a staining box (1), characterized in that: The front end of the dyeing box (1) is rotatably provided with a box door (11) through a hinge, the bottom of the dyeing box (1) is placed on a soaking box (12), four sliding rods (13) are fixed at the four corners of the soaking box (12) and the inner wall of the top of the dyeing box (1), and a placement component (3) is slidably installed on the surface of the sliding rods (13), and a partition component (4) is provided on the top of the placement component (3), the placement component (3) comprises a placement frame (31) and a lower frame (32), both sides of the placement frame (31) are provided with openings, and the lower frame (32) slides along both sides of the placement frame (31), and the partition component (4) comprises a top plate (41), the top plate (41) is slidably sleeved on the sliding rods (13) and located at the top of the placement frame (31), and an upper frame (43) is slidably installed on the top of the top plate (41), and the upper frame (43) is transmission-connected to the lower frame (32).

2. A Coomassie Brilliant Blue staining device according to claim 1, characterized in that: The partition assembly (4) also includes a transverse plate (47), the transverse plates (47) are fixed on both sides of the bottom of the top plate (41), and the transverse plates (47) are slidably sleeved on the slide rod (13), a screw rod device (48) is arranged inside the transverse plate (47), and a slide frame (49) that slides along the transverse plate (47) is meshed and sleeved on the outer surface of the screw rod device (48).

3. A Coomassie Brilliant Blue staining device according to claim 2, characterized in that: The placement component (3) further comprises an inserting plate (34). The inserting plates (34) are fixed at both ends of the placement frame (31), and the inserting plates (34) can be inserted into the interior of the sliding frame (49) by being snap-fitted.

4. A Coomassie Brilliant Blue staining device according to claim 3, characterized in that: The bottoms of both sides of the upper frame (43) are provided with sliding grooves (44), the interior of the sliding grooves (44) is slidably connected with a slider (45), the slider (45) is slidably plugged with a plug post (46), and the plug post (46) can be inserted into the corresponding position of the lower frame (32).

5. A Coomassie Brilliant Blue staining device according to claim 4, characterized in that: The top plate (41) is located on both sides of the upper frame (43) and is fixed with electric push rods (42), and the extended ends of the electric push rods (42) are fixedly connected to the upper frame (43).

6. A Coomassie Brilliant Blue staining device according to claim 5, characterized in that: The top plate (41) and the placement frame (31) are both provided with drain grooves on their surfaces.

7. A Coomassie Brilliant Blue staining device according to claim 6, characterized in that: A spray rack (2) is installed on the inner wall of the top of the dyeing box (1), and two rows of spray heads facing the drain grooves of the top plate (41) are arranged at the bottom of the spray rack (2).

8. A Coomassie Brilliant Blue staining device according to claim 7, characterized in that: A drainage pipe (33) is provided on a surface of one side of the lower frame (32) facing outward.

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