Test tube, test tube assembly and polymerase chain reaction device
By setting an opening and a support structure in the test tube, the detection part of the temperature detector and the inner wall of the test tube are kept a gap, which solves the problem of temperature detection error in the prior art and achieves higher detection accuracy.
Patent Information
- Application Number
- CN202323368227.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2023-12-11
- Publication Date
- 2025-03-11
- Estimated Expiration
- 2033-12-11
AI Technical Summary
In the prior art, the temperature detector is directly fixed to the inner wall of the test tube through a fixed structure, causing the detection end to come into contact with the inner wall of the test tube, resulting in a temperature measurement error.
A test tube is designed, by providing an opening on the test tube body, the first and second portions of the temperature detector are allowed to extend axially in the test tube, and a gap is maintained between the detection part and the inner wall of the test tube through the support part of the support structure.
It effectively avoids contact between the temperature detector and the inner wall of the test tube, improves the accuracy and accuracy of temperature detection, and reduces measurement errors.
Smart Images
Figure CN222593851U_ABST
Abstract
Description
Technical Field
[0001] The present disclosure relates to the technical field of experimental devices, and in particular to a test tube, a test tube assembly and a polymerase chain reaction device. Background Art
[0002] In biological and / or chemical reactions, it is sometimes necessary to control the reactants within a certain temperature range so that the reactants are at the required temperature, thereby promoting the progress of certain reactions.
[0003] For example, in the polymerase chain reaction (PCR), the DNA fragment to be amplified and the buffer solution need to be heated to 94-98° (i.e., the denaturation temperature) to deform the double-stranded DNA structure, and then the temperature of the DNA fragment to be amplified and the buffer solution is lowered to 50-65° (i.e., the annealing temperature) to allow the primer to specifically bind to the target DNA fragment, and then the temperature of the DNA fragment to be amplified and the buffer solution is raised to 72° (i.e., the extension temperature) to synthesize a new DNA chain, and the above three temperature cycles are repeated to achieve DNA amplification.
[0004] In order to facilitate real-time detection of the temperature of the reactants, a temperature detector is usually set in a test tube used to contain the reactants. In the related art, the temperature detector is usually fixed to the inner wall of the test tube by a fixed structure, which causes the detection end of the temperature detector to contact the inner wall of the test tube. However, since the temperature of the inner wall of the test tube and the temperature of the reactants contained in the test tube may be different, there is an error between the temperature measured by the temperature detector and the actual temperature of the reactants. Utility Model Content
[0005] The purpose of the present disclosure is to provide a test tube, a test tube assembly and a polymerase chain reaction device to solve the problems existing in the related art.
[0006] According to a first aspect of the present disclosure, there is provided a test tube, comprising:
[0007] A test tube body, the test tube body is provided with an opening so that the temperature detector can be placed in the test tube body through the opening and has a first portion extending along the axial direction of the test tube body and a second portion intersecting the first portion;
[0008] A support structure is installed in the test tube body and has a support portion, wherein the support portion is used to support the second part so that a gap can exist between the detection portion at the bottom of the first part and the inner wall of the test tube body.
[0009] Optionally, the support structure is configured to enable a central axis of the first portion to be coaxial with a central axis of the test tube body.
[0010] Optionally, a through cavity is formed on the support structure and penetrates the support structure along the axial direction of the test tube body, and the through cavity is used for the first part to pass through.
[0011] Optionally, the central axis of the through cavity is coaxial with the central axis of the test tube body.
[0012] Optionally, the opening is formed on the outer circumferential surface of the test tube body and extends axially along the test tube body, the top end of the opening is open and constitutes a first open end, the first open end is used for the second part to pass through and enter the opening, and the support portion is located between the through cavity and the opening.
[0013] Optionally, a groove is formed on the support structure, and the groove is located between the through-cavity and the opening. The side of the groove close to the through-cavity is connected to the through-cavity, and the side of the groove close to the opening is opposite to the opening and connected to the opening. The top end of the groove is open and constitutes a second open end, and the second open end is used for the second part to pass through and enter the groove, and the support portion is the bottom wall of the groove.
[0014] Optionally, a connection between the cavity wall of the through cavity and the bottom wall of the groove has a chamfer.
[0015] Optionally, the test tube body includes a tube body and a tube cover, the opening is formed on the outer circumferential surface of the tube body, the top of the tube body is formed with a tube mouth connected to the interior of the tube body, the support structure can enter the interior of the tube body through the tube mouth and have an interference fit with the inner wall of the tube body, and the tube cover is detachably connected to the tube body and can cover the tube mouth.
[0016] Optionally, the support structure comprises a mounting portion and a tube cover matching portion, the mounting portion is used for interference fit with the inner wall of the tube body, the tube cover matching portion is located on a side of the mounting portion close to the tube cover, and a slot is defined between the outer peripheral surface of the tube cover matching portion and the inner wall of the tube body;
[0017] The pipe cover comprises a cover body and a clamping portion formed on the cover body, wherein the cover body is used to cover the pipe opening, and the clamping portion can be clamped in the clamping groove.
[0018] Optionally, an end of the clamping portion away from the cover body is used to press the second part against the supporting portion.
[0019] Optionally, the support structure includes a mounting portion and an insertion portion, the mounting portion is used for interference fit with the inner wall of the tube body, the insertion portion is located on a side of the mounting portion away from the tube cover, and the cross-sectional area of the insertion portion is smaller than the cross-sectional area of the mounting portion.
[0020] Optionally, the supporting structure is made of polyetheretherketone material; or, the supporting structure is made of rubber material.
[0021] Optionally, there are multiple test tube bodies, the multiple test tubes are arranged at intervals, every two adjacent test tubes are connected to each other, and the support structure is installed in each test tube body.
[0022] According to a second aspect of the present disclosure, there is provided a test tube assembly, comprising a temperature detector and the test tube as described above.
[0023] Optionally, the temperature detector includes a detection part and a wiring harness electrically connected to the detection part, the detection part and at least part of the wiring harness can be placed into the test tube body of the test tube through an opening on the test tube body of the test tube, the wiring harness located in the test tube body has a first part extending along the axial direction of the test tube body and a second part intersecting with the first part, the second part is supported on a supporting part of the support structure of the test tube.
[0024] Optionally, the temperature detector comprises a K-type thermocouple.
[0025] According to a third aspect of the present disclosure, a polymerase chain reaction device is provided, comprising the test tube assembly as described above.
[0026] Through the above technical solution, since the temperature detector is installed in the test tube body through the supporting structure, and the supporting part of the supporting structure can support the second part of the temperature detector, the detection part of the temperature detector for measuring the temperature of the reactant can have a gap with the inner wall of the test tube body, that is, the detection part of the temperature detector will not contact the inner wall of the test tube body. In this way, the inner wall of the test tube body with a temperature different from that of the reactant will not interfere with the detection result of the detection part, and the detection part can accurately measure the real-time temperature of the reactant, which is beneficial to improving the detection accuracy of the temperature detector.
[0027] For example, in the polymerase chain reaction (PCR), the test tube body contains the DNA fragments to be amplified and the buffer solution, and the temperature detector is installed in the test tube body through the support structure. Due to the supporting effect of the support structure, the detection part will not directly contact the inner wall of the test tube body. The detection part of the temperature detector can be located in the liquid surface of the DNA fragments to be amplified and the buffer solution, and accurately measure the temperature of the DNA fragments to be amplified and the buffer solution. In this way, by reasonably adjusting the heating device used to heat the test tube, the temperature of the DNA fragments to be amplified and the buffer solution can be cycled between the denaturation temperature, the annealing temperature and the extension temperature, thereby achieving DNA amplification.
[0028] Compared with the technical solution in the related art in which the temperature detector is directly fixed on the inner wall of the test tube through a fixed structure, in the test tube provided by the present invention, the temperature detector can be stably installed in the test tube body through the supporting structure, and the detection part of the temperature detector will not directly contact the inner wall of the test tube body. The temperature detector has high detection accuracy and small error, which effectively avoids the inner wall with a different temperature from the reactant interfering with the detection part, resulting in the situation where the temperature of the reactant measured by the detection part is different from the actual temperature of the reactant.
[0029] Other features and advantages of the present disclosure will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS
[0030] The accompanying drawings are used to provide a further understanding of the present disclosure and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the present disclosure but do not constitute a limitation of the present disclosure. In the accompanying drawings:
[0031] Figure 1 is a schematic cross-sectional view of a test tube provided in an exemplary embodiment of the present disclosure, wherein a temperature detector is also shown.
[0032] Figure 2 It is a schematic diagram of the three-dimensional structure of a support structure of a test tube provided in an exemplary embodiment of the present disclosure.
[0033] Figure 3 It is a schematic diagram of the three-dimensional structure of a test tube assembly provided in an exemplary embodiment of the present disclosure, wherein the support structure is installed in the test tube body.
[0034] Figure 4 It is a schematic diagram of an explosion of a test tube assembly provided in an exemplary embodiment of the present disclosure.
[0035] Description of Reference Numerals
[0036] 1-test tube body; 11-tube body; 111-opening; 112-first open end; 113-tube mouth; 12-tube cover; 121-cover body; 122-clamping part; 2-support structure; 21-support part; 22-groove; 23-second open end; 24-installation part; 25-tube cover matching part; 26-slot; 27-insertion part; 28-chamfer; 29-through cavity; 3-temperature detector; 31-detection part; 32-wiring harness; 321-first part; 322-second part. DETAILED DESCRIPTION
[0037] The specific implementation of the present disclosure is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described herein is only used to illustrate and explain the present disclosure, and is not used to limit the present disclosure.
[0038] In the present disclosure, unless otherwise stated, the orientation or positional relationship indicated by the directional words such as "top" and "bottom" is defined based on the drawing direction shown in the corresponding drawings. It is only for the convenience of describing the present disclosure and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, specific orientation structure and operation. Therefore, it cannot be understood as a limitation on the present disclosure. The terms "inside and outside" refer to the inside and outside of the corresponding structural contour. The "axial direction" in the drawings and text generally refers to the axial direction relative to the contour of the test tube itself, and can also refer to the up and down direction of the test tube body. For details, please refer to Figure 1 direction shown.
[0039] In addition, it should be noted that the terms used, such as "first", "second", etc., are used to distinguish one element from another element, and do not have order or importance. In addition, in the description with reference to the drawings, the same number in different drawings represents the same element.
[0040] In the description of the present disclosure, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "connect", "connected", and "installed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present disclosure can be understood according to specific circumstances.
[0041] like Figures 1 to 4 As shown, according to the first aspect of the present disclosure, there is provided a test tube, comprising a test tube body 1 and a support structure 2, wherein the test tube body 1 is provided with an opening 111 so that a temperature detector 3 can be placed into the test tube body 1 through the opening 111 and comprises a first portion 321 extending along the axial direction of the test tube body 1 and a second portion 322 intersecting with the first portion 321, the support structure 2 is installed in the test tube body 1 and comprises a support portion 21, the support portion 21 is used to support the second portion 322 so that a gap can be formed between the detection portion 31 at the bottom of the first portion 321 and the inner wall of the test tube body 1.
[0042] In the above-mentioned test tube, the temperature detector 3 can be installed in the test tube through the opening 111 on the test tube body 1, and the temperature detector 3 located in the test tube body 1 can have a first part 321 extending along the axial direction of the test tube body 1 and a second part 322 intersecting with the first part 321, wherein the detection part 31 for detecting the temperature of the reactant is located at the bottom of the first part 321, and the second part 322 of the temperature detector 3 can extend out of the test tube body 1 through the opening 111 and be electrically connected to the data acquisition instrument and / or the controller of the heating component, so as to feed back the collected temperature data to the data acquisition instrument and / or the controller of the heating component.
[0043] Through the above technical scheme, since the temperature detector 3 is installed in the test tube body 1 through the supporting structure 2, and the supporting part 21 of the supporting structure 2 can support the second part 322 of the temperature detector 3, the detection part 31 used to measure the temperature of the reactant in the temperature detector 3 can have a gap with the inner wall of the test tube body 1, that is, the detection part 31 of the temperature detector 3 will not contact the inner wall of the test tube body 1. In this way, the inner wall of the test tube body 1 with a temperature different from that of the reactant will not interfere with the detection result of the detection part 31. The detection part 31 can accurately measure the real-time temperature of the reactant, which is beneficial to improving the detection accuracy of the temperature detector 3.
[0044] For example, in the polymerase chain reaction (PCR), the test tube body 1 contains the DNA fragment to be amplified and the buffer solution, and the temperature detector 3 is installed in the test tube body 1 through the support structure 2. Due to the supporting effect of the support structure 2, the detection part 31 will not directly contact the inner wall of the test tube body 1. The detection part 31 of the temperature detector 3 can be located in the liquid surface of the DNA fragment to be amplified and the buffer solution, and accurately measure the temperature of the DNA fragment to be amplified and the buffer solution. In this way, by reasonably adjusting the heating device for heating the test tube, the temperature of the DNA fragment to be amplified and the buffer solution can be cycled between the denaturation temperature, the annealing temperature and the extension temperature, thereby realizing DNA amplification.
[0045] Compared with the technical solution in the related art in which the temperature detector is directly fixed on the inner wall of the test tube through a fixed structure, in the test tube provided by the present invention, the temperature detector 3 can be stably installed in the test tube body 1 through the support structure 2, and the detection part 31 of the temperature detector 3 will not directly contact the inner wall of the test tube body 1. The temperature detector 3 has high detection accuracy and small error, which effectively avoids the inner wall with a different temperature from the reactant interfering with the detection part 31, resulting in the situation that the temperature of the reactant measured by the detection part 31 is different from the actual temperature of the reactant.
[0046] Here, it should be noted that the present disclosure does not limit the use of the above test tube, and it can be used in any experiment that requires the use of a test tube and detects the temperature of the reactants in the test tube. For example, the above test tube can be used in polymerase chain reaction (PCR) to detect the real-time temperature of the DNA fragment to be amplified and the buffer, and cooperate with other devices to achieve accurate regulation of the temperature of the DNA fragment to be amplified and the buffer, thereby achieving DNA amplification. As other embodiments, the above test tube can also be used in other experiments such as the silver mirror experiment and the experiment to explore the effect of temperature on enzyme activity.
[0047] In addition, the present disclosure does not limit the specific type of the temperature detector 3. For example, the temperature detector may be a thermocouple or a temperature sensor. The temperature detector 3 may have a first portion 321 and a second portion 322 intersecting each other before being placed in the test tube body 1. After the temperature detector 3 is placed in the test tube body 1, the second portion 322 of the temperature detector 3 may be supported on the support portion 21 of the support structure 2, so that the temperature detector 3 can have a stable posture in the test tube body 1; or Figure 1 As shown, the temperature detector 3 may also include a detection part 31 and a wiring harness 32 connected to the detection part 31. After the detection part 31 and at least part of the wiring harness 32 are placed in the test tube body 1, the wiring harness 32 is supported on the support part 21 of the support structure 2. The support part 21 bends the wiring harness 32 and has a first part 321 and a second part 322. The support part 21 is supported on the second part 322.
[0048] In order to further improve the detection accuracy of the temperature detector 3, optionally, as Figure 1 As shown, the support structure 2 is configured to enable the central axis of the first portion 321 to be coaxial with the central axis of the test tube body 1. In this way, along the axial direction of the test tube body 1, the central axis of the detection portion 31 of the temperature detector 3 can be coaxial with the rotational central axis of the test tube body 1. In this way, on the same horizontal plane, each position of the detection portion 31 can be equal to the distance between the inner wall of the test tube body 1 surrounding the detection portion 31. By adjusting the height of the detection portion 31, the detection portion 31 can also be located at the center of the reactant contained in the test tube body 1, so that the detection portion 31 can accurately measure the real-time temperature of the reactant.
[0049] Here, it should be noted that the present disclosure does not limit the specific structure of the support structure 2, as long as the support structure 2 can support the temperature detector 3 and can ensure that there is a gap and no contact between the detection part 31 of the temperature detector 3 and the inner wall of the test tube body 1, for example, the above-mentioned support structure 2 can be formed as a hanging ring or a support seat, etc.
[0050] For the implementation mode in which the supporting structure 2 is formed as a hanging ring, the second part 322 of the temperature detector 3 can pass through the hanging ring and be mounted on the hanging ring, and the first part 321 of the temperature detector 3 passes through the hanging ring and is hung in the test tube body 1, so that there is a gap between the detection part 31 at the bottom of the first part 321 and the inner wall of the test tube body 1.
[0051] For the embodiment in which the support structure 2 is formed as a support seat, in order to facilitate the first part 321 of the temperature detector 3 to be installed in the test tube body 1, optionally, as Figure 1 and Figure 2As shown, a through cavity 29 is formed on the support structure 2 and penetrates the support structure 2 along the axial direction of the test tube body 1, and the through cavity 29 is used for the first part 321 to pass through. In this way, the first part 321 of the temperature detector 3 is inserted into the test tube body 1 or taken out from the test tube body 1 through the through cavity 29 on the support structure 2, and the installation of the temperature detector 3 in the test tube body 1 is relatively simple. In addition, the through cavity 29 can limit the first part 321 of the temperature detector 3, so that the temperature detector 3 can be installed in the test tube body 1 relatively stably, and the first part 321 of the temperature detector 3 is prevented from shaking and contacting the inner wall of the test tube body 1.
[0052] In order to make the central axis of the first part 321 of the temperature detector 3 coaxial with the central axis of the test tube body 1, optionally, as Figure 1 As shown, the central axis of the through cavity 29 is coaxial with the central axis of the test tube body 1. In this way, the operator does not need to measure the installation position of the temperature detector 3 in the test tube body 1, but only needs to pass the first part 321 of the temperature detector 3 through the through cavity 29 on the support structure 2 and install it into the test tube body 1, so that the detection part 31 arranged at the bottom of the first part 321 of the temperature detector 3 can be coaxial with the central axis of the test tube body 1.
[0053] The present disclosure does not limit the specific structure of the test tube body 1. In order to facilitate the installation of the temperature detector 3 into the test tube body 1, optionally, as Figure 1 and Figure 4 As shown, the opening 111 is formed on the outer peripheral surface of the test tube body 1 and extends along the axial direction of the test tube body 1. The top end of the opening 111 is open and constitutes a first open end 112. The first open end 112 is used for the second part 322 to pass through and enter the opening 111. The support portion 21 is located between the through cavity 29 and the opening 111. In this way, when the temperature detector 3 is placed in the test tube body 1, the second part 322 of the temperature detector 3 can at least partially pass through the first open end 112 of the opening 111, so that the first part 321 of the temperature detector 3 intersecting with the second part 322 can pass through the through cavity 29 and be installed in the test tube body 1, and the first part 321 can be supported on the support portion 21, which is convenient for the installation of the temperature detector 3.
[0054] Furthermore, the above technical solution also avoids the situation where the first part 321 and / or the second part 322 of the temperature detector 3 are made of rigid material and cannot be deformed, resulting in the detection part 31 of the temperature detector 3 being unable to be installed in the test tube body 1.
[0055] In addition, since the support portion 21 is located between the through cavity 29 and the opening 111, when the first portion 321 of the temperature detector 3 passes through the through cavity 29 and is installed in the test tube body 1, the second portion 322 of the temperature detector 3 can be directly overlapped on the support portion 21 of the support structure 2, and the support portion 21 can support the temperature detector 3, thereby achieving the relative fixation of the position of the detection portion 31 in the test tube body 1.
[0056] Alternatively, if Figure 2 As shown, a groove 22 is formed on the support structure 2, and the groove 22 is located between the through cavity 29 and the opening 111. The side of the groove 22 close to the through cavity 29 is connected to the through cavity 29, and the side of the groove 22 close to the opening 111 is opposite to the opening 111 and connected to the opening 111. The top of the groove 22 is open and forms a second open end 23, and the second open end 23 is used for the second part 322 to pass through and enter the groove 22. The support part 21 is the bottom wall of the groove 22. When the temperature detector 3 is installed in the test tube body 1, the second part 322 of the temperature detector 3 can partially pass through the second open end 23 formed on the groove 22 of the support structure 2 and be supported on the bottom wall of the groove 22.
[0057] In addition, since the supporting portion 21 is the bottom wall of the groove 22, when the first portion 321 of the temperature detector 3 passes through the through cavity 29 and is installed in the test tube body 1, the bottom wall of the groove 22 and the bottom wall of the opening 111 of the test tube body 1 can jointly support the second portion 322 of the temperature detector 3, and the side walls of the groove 22 and the side walls of the opening 111 can limit the second portion 322 of the temperature detector 3, so that the temperature detector 3 is reliably fixed in the test tube body 1, and the detection portion 31 is not easy to shake in the test tube body 1.
[0058] In order to prevent the support structure 2 from damaging the temperature detector 3, optionally, the connection between the cavity wall of the through cavity 29 and the bottom wall of the groove 22 has a chamfer 28. The chamfer 28 can prevent the connection between the cavity wall of the through cavity 29 and the bottom wall of the groove 22 from rubbing against the inflection of the first part 321 and the second part 322 of the temperature detector 3, thereby causing scratches or even damage to the temperature detector 3.
[0059] The present disclosure does not limit the specific structure of the test tube body 1, as long as the test tube body 1 can be used as a reaction container for accommodating reactants and can facilitate the installation of the temperature detector 3. As an embodiment of the present disclosure, the test tube body 1 includes a tube body 11 and a tube cover 12, an opening 111 is formed on the outer peripheral surface of the tube body 11, and a pipe mouth 113 connected to the interior of the tube body 11 is formed at the top of the tube body 11, the support structure 2 can enter the interior of the tube body 11 through the pipe mouth 113 and have an interference fit with the inner wall of the tube body 11, and the tube cover 12 is detachably connected to the tube body 11 and can cover the pipe mouth 113.
[0060] The tube cover 12 can prevent the tube body 11 from pollution, evaporation and leakage. The tube cover 12 can protect the reactants and effectively prevent the reactants from being polluted by the external environment, affecting the experimental results or even making it impossible to conduct the experiment normally. At the same time, the tube cover 12 can also improve the safety of the experiment.
[0061] In addition, since an interference fit is formed between the support structure 2 and the inner wall of the tube body 11, the support structure 2 is reliably fixed in the tube body 11 and is not easy to shake in the tube body 11, which effectively avoids the situation where the support structure 2 is unreliably fixed and the support structure 2 becomes loose in the tube body 11, causing the detection part 31 of the temperature detector 3 to be displaced in the tube body 11, thereby affecting the detection result of the temperature detector 3.
[0062] The present disclosure does not limit the specific structure of the support structure 2. In order to facilitate the installation of the support structure 2 into the tube body 11, optionally, as Figure 1 and Figure 2 As shown, the support structure 2 includes a mounting portion 24 and a tube cover matching portion 25. The mounting portion 24 is used for interference fit with the inner wall of the tube body 11. The tube cover matching portion 25 is located on the side of the mounting portion 24 close to the tube cover 12. A clamping groove 26 is defined between the outer peripheral surface of the tube cover matching portion 25 and the inner wall of the tube body 11. The tube cover 12 includes a cover body 121 and a clamping portion 122 formed on the cover body 121. The cover body 121 is used to cover the tube opening 113. The clamping portion 122 can be clamped in the clamping groove 26. The tube cover 12 is clamped in the clamping groove 26 between the support structure 2 and the tube body 11 through the clamping portion 122 on the cover body 121. The tube cover 12 is reliably fixed on the tube body 11, which effectively avoids the situation that the tube cover 12 is not reliably fixed on the tube body 11 and the tube cover 12 is easy to fall off the tube body 11.
[0063] In addition, the tube cover 12 clamped on the tube body 11 can also play a role in limiting the support structure 2. When the reactants inside the tube body 11 react, the pressure inside the tube body 11 is greater than the external environmental pressure, and the support structure 2 is subjected to a force that causes the support structure 2 to move upward, the support structure 2 squeezes the tube cover 12 upward. The tube cover 12 can apply a reaction force to squeeze the support structure 2, effectively preventing the support structure 2 from being displaced inside the tube body 11, causing the detection part 31 of the temperature detector 3 to be displaced inside the tube body 11, thereby affecting the detection result of the temperature detector 3.
[0064] In order to further prevent the detection part 31 of the temperature detector 3 from being displaced in the tube body 11, which may cause errors in the detection result of the temperature detector 3, optionally, one end of the clamping part 122 away from the cover body 121 is used to press the second part 322 against the support part 21. In this way, the second part 322 of the temperature detector 3 can be clamped between the support part 21 and the cover body 121, the temperature detector 3 is more reliably fixed in the test tube body 1, and the second part 322 of the temperature detector 3 is not easily moved by force, which effectively prevents the second part 322 of the temperature detector 3 from being displaced in the test tube body 1, causing the first part 321 to be displaced, and affecting the detection result of the temperature detector 3.
[0065] In order to facilitate the installation of the support structure 2 into the tube body 11, optionally, as Figure 1 , Figure 2 and Figure 4 As shown, the support structure 2 includes a mounting portion 24 and an insertion portion 27. The mounting portion 24 is used for interference fit with the inner wall of the tube body 11. The insertion portion 27 is located on the side of the mounting portion 24 away from the tube cover 12. The cross-sectional area of the insertion portion 27 is smaller than the cross-sectional area of the mounting portion 24. The insertion portion 27 with a smaller area can guide the support structure 2. When the support structure 2 is installed, the insertion portion 27 can be inserted into the tube body 11 first, and then the mounting portion 24 can be inserted into the tube body 11, so that the mounting portion 24 and the inner wall of the tube body 11 form an interference fit, thereby realizing the installation of the support structure 2 in the tube body 11.
[0066] Here, it can be understood that, for the implementation mode in which a through cavity 29 is formed on the support structure 2 and penetrates the support structure 2 along the axial direction of the test tube body 1, the through cavity 29 penetrates the insertion portion 27, the mounting portion 24 and the tube cover matching portion 25, and the through cavity 29 is relatively long. The longer through cavity 29 can also play a limiting role on the first part 321 of the temperature detector 3. The first part 321 of the temperature detector 3 is not easy to shake in the through cavity 29, which effectively avoids the first part 321 of the temperature detector 3 from shaking easily in the through cavity 29, causing the detection part 31 to shake in the test tube body 1 and contact the inner wall of the test tube body 1.
[0067] The present disclosure does not limit the material of the support structure 2, as long as the support structure 2 is inside the test tube body 1 and does not react with the reactants contained in the test tube body 1 and is not corroded by the reactants. As an embodiment of the present disclosure, the above-mentioned support structure 2 can be made of polyetheretherketone material.
[0068] Here, it should be noted that the polyetheretherketone material (Peek) is usually a black opaque material. Polyetheretherketone is a polymer composed of repeating units containing one ketone bond and two ether bonds in the main chain structure. It belongs to a special polymer material. It has physical and chemical properties such as high temperature resistance and chemical corrosion resistance. It can be used as a high temperature resistant structural material and electrical insulation material, and can also be composited with glass fiber or carbon fiber to prepare reinforcement material. The polyetheretherketone material itself is not easy to react with the reactants, and the polyetheretherketone material itself is not easily corroded by the reactants, which effectively improves the service life of the support structure 2.
[0069] As another embodiment, the supporting structure 2 may also be made of rubber material.
[0070] The present disclosure does not limit the number of test tube bodies 1. To improve the efficiency of the experiment, optionally, Figure 3 and Figure 4 As shown, there are multiple test tube bodies 1, which are arranged at intervals, and each two adjacent test tubes are connected to each other, and a support structure 2 is installed in each test tube body 1. In other words, the test tubes can be row-by-row test tubes. Multiple tube bodies 11 can accommodate the same and / or different reactants, effectively improving the efficiency of the experiment.
[0071] Here, it can be understood that, for an implementation mode in which there are multiple test tube bodies 1, multiple test tubes are arranged at intervals, and every two adjacent test tubes are interconnected, the number of tube covers 12 used to cover the tube opening 113 on the tube body 11 is also correspondingly multiple, the multiple tube covers 12 correspond to the tube body 11 one by one, and the cover bodies 121 of every two adjacent tube covers 12 can also be interconnected as a whole.
[0072] For example, Figure 3 and Figure 4 As shown, the number of the test tube bodies 1 may be eight, the eight test tubes are arranged at intervals, every two adjacent test tubes are connected to each other, and a support structure 2 is installed in each test tube body 1 .
[0073] The present disclosure does not limit the material of the test tube. Optionally, the test tube is made of medical high-transparency polypropylene. Medical high-transparency polypropylene has high stability and heat resistance. The test tube has good performance and can be used to accommodate a variety of different reactants.
[0074] According to a second aspect of the present disclosure, there is provided a test tube assembly, comprising a temperature detector 3 and the test tube as described above.
[0075] The test tube assembly has all the beneficial effects of the above test tubes, which will not be described in detail here.
[0076] The present disclosure does not limit the specific function of the temperature detector 3, as long as the temperature detector 3 can be installed in the test tube and can measure the temperature of the reactant contained in the test tube. As an embodiment of the present disclosure, the temperature detector 3 includes a detection part 31 and a wiring harness 32 electrically connected to the detection part 31, the detection part 31 and at least part of the wiring harness 32 can be placed in the test tube body 1 through the opening 111 on the test tube body 1 of the test tube, the wiring harness 32 located in the test tube body 1 has a first part 321 extending along the axial direction of the test tube body 1 and a second part 322 intersecting with the first part 321, and the second part 322 is supported on the support part 21 of the support structure 2 of the test tube.
[0077] The present disclosure does not limit the specific type of the temperature detector 3, as long as the detection frequency and detection accuracy of the temperature detector 3 can meet the requirements of the experiment. As an embodiment of the present disclosure, the temperature detector 3 includes a K-type thermocouple. The K-type thermocouple is composed of two metal materials of different components to form a closed loop. When the temperature of the reactant contained in the test tube changes, due to the existence of the temperature gradient, a current will pass through the closed loop. By reading the current signal in the closed loop, the temperature of the reactant contained in the test tube can be obtained.
[0078] Here, it can be understood that, for the temperature detector 3 including the detection part 31, and the temperature detector 3 is an embodiment of a K-shaped thermocouple, the temperature sensing element on the K-shaped thermocouple is the detection part 31 of the temperature detector 3, and in the K-shaped thermocouple, the part of the wiring harness 32 located in the test tube body 1 extending along the axial direction of the test tube body 1 constitutes the first part 321 of the temperature detector 3, and the part supported on the support part 21 of the support structure 2 constitutes the second part 322 of the temperature detector 3.
[0079] According to a third aspect of the present disclosure, a polymerase chain reaction device is provided, comprising the test tube assembly as described above.
[0080] The polymerase chain reaction device has all the beneficial effects of the above-mentioned test tube, which will not be described in detail here.
[0081] The preferred embodiments of the present disclosure are described in detail above in conjunction with the accompanying drawings; however, the present disclosure is not limited to the specific details in the above embodiments. Within the technical concept of the present disclosure, a variety of simple modifications can be made to the technical solution of the present disclosure, and these simple modifications all fall within the protection scope of the present disclosure.
[0082] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present disclosure will not further describe various possible combinations.
[0083] In addition, various embodiments of the present disclosure may be arbitrarily combined, and as long as they do not violate the concept of the present disclosure, they should also be regarded as the contents disclosed by the present disclosure.
Claims
1. A test tube, characterized in that: include: A test tube body, the test tube body is provided with an opening so that the temperature detector can be placed in the test tube body through the opening and has a first portion extending along the axial direction of the test tube body and a second portion intersecting the first portion; A support structure is installed in the test tube body and has a support portion, wherein the support portion is used to support the second part so that a gap can exist between the detection portion at the bottom of the first part and the inner wall of the test tube body.
2. The test tube according to claim 1, characterized in that The support structure is configured to enable a central axis of the first portion to be coaxial with a central axis of the test tube body.
3. The test tube according to claim 1, characterized in that A through cavity is formed on the support structure and penetrates the support structure along the axial direction of the test tube body, and the through cavity is used for the first part to pass through.
4. The test tube according to claim 3, characterized in that The central axis of the through cavity is coaxial with the central axis of the test tube body.
5. The test tube according to claim 3, characterized in that The opening is formed on the outer peripheral surface of the test tube body and extends along the axial direction of the test tube body. The top end of the opening is open and constitutes a first open end, and the first open end is used for the second part to pass through and enter the opening. The support part is located between the through cavity and the opening.
6. The test tube according to claim 5, characterized in that A groove is formed on the supporting structure, and the groove is located between the through-cavity and the opening. The side of the groove close to the through-cavity is connected with the through-cavity, and the side of the groove close to the opening is opposite to the opening and connected with the opening. The top end of the groove is open and constitutes a second open end, and the second open end is used for the second part to pass through and enter the groove. The supporting part is the bottom wall of the groove.
7. The test tube according to claim 6, characterized in that A connection between the cavity wall of the through cavity and the bottom wall of the groove has a chamfer.
8. The test tube according to claim 3, characterized in that The test tube body includes a tube body and a tube cover, the opening is formed on the outer circumferential surface of the tube body, the top end of the tube body is formed with a tube mouth connected to the interior of the tube body, the support structure can enter the interior of the tube body through the tube mouth and have an interference fit with the inner wall of the tube body, and the tube cover is detachably connected to the tube body and can cover the tube mouth.
9. The test tube according to claim 8, characterized in that The support structure comprises a mounting portion and a tube cover matching portion, wherein the mounting portion is used for interference fitting with the inner wall of the tube body, the tube cover matching portion is located on a side of the mounting portion close to the tube cover, and a card slot is defined between the outer peripheral surface of the tube cover matching portion and the inner wall of the tube body; The pipe cover comprises a cover body and a clamping portion formed on the cover body, wherein the cover body is used to cover the pipe opening, and the clamping portion can be clamped in the clamping groove.
10. The test tube according to claim 9, characterized in that One end of the clamping portion away from the cover body is used to press the second portion against the supporting portion.
11. The test tube according to claim 8, characterized in that The support structure includes a mounting portion and an insertion portion, wherein the mounting portion is used for interference fit with the inner wall of the tube body, and the insertion portion is located on a side of the mounting portion away from the tube cover, and the cross-sectional area of the insertion portion is smaller than the cross-sectional area of the mounting portion.
12. The test tube according to any one of claims 1 to 11, characterized in that The supporting structure is made of polyetheretherketone material; or, the supporting structure is made of rubber material.
13. The test tube according to any one of claims 1 to 11, characterized in that There are multiple test tube bodies, which are arranged at intervals, and every two adjacent test tubes are connected to each other. The support structure is installed in each test tube body.
14. A test tube assembly, characterized in that: The test tube comprises a temperature detector and any one of claims 1-13.
15. The test tube assembly according to claim 14, characterized in that The temperature detector includes a detection part and a wiring harness electrically connected to the detection part, the detection part and at least part of the wiring harness can be placed into the test tube body through an opening on the test tube body of the test tube, the wiring harness located in the test tube body has a first part extending along the axial direction of the test tube body and a second part intersecting with the first part, the second part is supported on a supporting part of the support structure of the test tube.
16. The test tube assembly according to claim 14 or 15, characterized in that: The temperature detector includes a K-type thermocouple.
17. A polymerase chain reaction device, characterized in that: A test tube assembly comprising any one of claims 14-16.