Mass spectrum microbiological identification sample protein pretreatment device

Abstract

The utility model discloses a mass spectrum microbiological identification sample protein pretreatment device which comprises a tube body, wherein the tube body is a non-hollow container; the tube cover is detachably mounted at the opening of the tube body; the filtering assembly is detachably arranged in the pipe body, and the filtering assembly comprises a filtering cone, a first filtering part, a communicating pipe and a second filtering part; the filter cone is sequentially communicated with the first filter part, the communicating pipe and the second filter part; the filter assembly can be completed only by installing the first filter membrane and the second filter membrane, operation is easy and convenient, and time and labor are saved; and after cleaning, the first filter membrane and the second filter membrane can be repeatedly used after being replaced, so that the detection cost is reduced.

Description

Technical Field

[0001] The utility model relates to a mass spectrometry microorganism identification sample protein pretreatment device, belonging to the field of microorganism identification. Background Art

[0002] Traditional microbial identification methods rely on phenotypes and physiological and biochemical reactions, and generally have problems such as being time-consuming and having poor specificity, and cannot meet the needs of microbial safety assurance under the new situation.

[0003] Molecular biology methods, such as PCR rapid detection technology and nucleic acid sequencing technology, although more specific and sensitive than traditional methods, are relatively complex to operate and require high professional qualities of experimental personnel, and cannot be widely promoted in grassroots laboratories.

[0004] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a laser desorption rapid bacterial species identification technology that can quickly identify microbial species within minutes. It is fast, accurate, and has low daily costs. The matrix solution, strain culture medium, culture time, and sample pretreatment method are the main factors affecting the mass spectrometry characteristic protein spectrum. Different types of microorganisms have different cell wall structures and compositions. Therefore, different sample pretreatment methods have different degrees of bacterial protein release, which often affects the identification results.

[0005] Currently, there are no commercial products for biosafety extraction of samples for mass spectrometry microbial detection worldwide. In the process of preparing samples for the MALDI-TOF MS method reported in the prior art, there are biosafety risks. This is because the microorganisms to be identified are all unknown microorganisms. Operators cannot judge whether the microorganism is highly pathogenic based on the appearance of the strain, nor can they predict in advance whether the microorganism to be tested has capsules or spores. Sample pretreatment can only be performed uniformly according to a biosafety level. For microorganisms such as thick-walled and spore-bearing microorganisms, the current extraction device cannot easily and quickly kill or isolate pathogens, which poses a biosafety hazard.

[0006] Therefore, this device aims to design a pretreatment device that can quickly isolate pathogens. Utility Model Content

[0007] In view of the deficiencies in the prior art, the utility model aims to provide a mass spectrometry microbial identification sample protein pretreatment device to solve the problem.

[0008] In order to achieve the above-mentioned object, the utility model is realized by the following technical scheme: a mass spectrometry microorganism identification sample protein pretreatment device, comprising a tube body, a tube cover, and a filter assembly, wherein the tube cover is detachably mounted on the opening of the tube body; the filter assembly is detachably arranged in the tube body, and the filter assembly comprises a filter bucket, a first filter portion arranged at the bottom of the filter bucket, a connecting pipe arranged at the bottom of the first filter portion, and a second filter portion arranged at the bottom of the connecting pipe;

[0009] The first filter part and the second filter part are used to filter the pretreatment reagent;

[0010] The first filter part and the second filter part are used for filtering.

[0011] Preferably, a flexible connecting belt is connected between the tube body and the tube cover.

[0012] Preferably, the tube cover is provided with a first plug, and the first plug will be inserted into the filter funnel.

[0013] Preferably, a limiting ring is provided on the filter bucket, and the diameter of the limiting ring is larger than the diameter of the opening.

[0014] Preferably, the first filter portion comprises a first filter membrane, and the filter membrane is arranged between the filter bowl and the connecting tube, wherein the diameter of the filter membrane is larger than the diameter of the connecting tube.

[0015] Preferably, a fence is provided at the inlet of the connecting pipe.

[0016] Preferably, the second filter portion comprises a second filter membrane, and the second filter membrane is located at the outlet of the connecting pipe.

[0017] Preferably, the second filter part comprises a shell for placing the second filter membrane, the top of the shell is connected to the connecting pipe, and the bottom of the shell has a semi-open recess, and the second filter membrane is placed in the recess.

[0018] Preferably, a protrusion is provided on the inner edge of the recess, and the protrusion supports the second filter membrane.

[0019] Beneficial Effects

[0020] The utility model only needs to install the first filter membrane and the second filter membrane to complete the filtration assembly, which is easy to operate, time-saving and labor-saving; and after cleaning, the first filter membrane and the second filter membrane can be replaced and reused, thereby reducing the detection cost. It can effectively avoid the potential hazards brought by biosafety, and all intracellular proteins of microorganisms can be obtained through a simple device, which promotes the research of microbial sample pretreatment methods and has a great role in promoting mass spectrometry microbial sample preparation technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Other features, objects and advantages of the present invention will become more apparent by reading the detailed description of non-limiting embodiments with reference to the following drawings:

[0022] Figure 1 This is a schematic diagram of the structure of a mass spectrometry microbial identification sample protein pretreatment device of the utility model;

[0023] Figure 2 This is a structural schematic diagram of the first filter unit of the utility model;

[0024] Figure 3 It is a structural schematic diagram of the second filter part of the utility model. DETAILED DESCRIPTION

[0025] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0026] See also Figure 1-Figure 3 The utility model provides a technical solution of a mass spectrometry microbial identification sample protein pretreatment device: comprising a tube body 1, a tube cover 2, and a filtering component 3.

[0027] The tube body 1 is a non-hollow container. Preferably, the tube body 1 is made of visible glass so that the inspector can visually observe the inside of the tube body 1 .

[0028] The tube cover 2 is detachably mounted on the opening A of the tube body 1. Preferably, a flexible connecting belt 4 is connected between the tube body 1 and the tube cover 2. Specifically, the two ends of the connecting belt 4 are respectively fixed to the tube body 1 and the tube cover 2, so that the tube cover 2 can move to a certain extent, thereby being detachably mounted on the opening A of the tube body 1.

[0029] The filter assembly 3 is detachably disposed in the tube body 1 , so that the filter assembly 3 can be directly taken out after the filtering is completed.

[0030] The filter assembly 3 further comprises a filter cup 31, a first filter part 32, a connecting pipe 33, and a second filter part 34; the filter cup 31 is sequentially connected with the first filter part 32, the connecting pipe 33, and the second filter part 34. In one embodiment, the pretreatment reagent is poured into the filter cup 31, the pretreatment reagent is filtered for the first time through the first filter part 32, the filtered pretreatment reagent flows into the second filter part 34 through the connecting pipe 33, is filtered for the second time by the second filter part 34, and then flows into the bottom of the tube body 1. After the pretreatment reagent has flowed out, the filter assembly 3 can be taken out.

[0031] See also Figure 2Preferably, the first filter part 32 includes a first filter membrane 321, which is arranged between the filter bucket 31 and the connecting pipe 33, and filtering is achieved by the first filter membrane 321. In one embodiment, in order to prevent the filter membrane from sinking into the connecting pipe 33 and falling into the second filter part 34, the diameter of the filter membrane is larger than the diameter of the connecting pipe 33, so that the filter membrane can be prevented from being impacted by the reagent and sinking into the connecting pipe 33. In another embodiment, a fence 331 is provided at the entrance of the connecting pipe 33, and the fence 331 plays a role of supporting and blocking the filter membrane, so that the filter membrane can be further prevented from being impacted by the reagent and sinking into the connecting pipe 33.

[0032] To further prevent the filter assembly 3 from sinking into the bottom of the tube body 1, a limiting ring 331 is provided on the filter bucket 31, and the diameter of the limiting ring 331 is larger than the diameter of the opening A. In this way, after the filter assembly 3 is placed on the tube body 1, the limiting ring 331 supports the opening A of the tube body 1, thereby preventing the filter assembly 3 from sinking into the bottom of the tube body 1.

[0033] The second filter unit 34 includes a second filter membrane 341, and the second filter membrane 341 is located at the outlet of the connecting pipe 33. The second filter membrane 341 performs secondary filtration.

[0034] See also Figure 3 In one embodiment, the second filter portion 34 includes a shell 342 for placing a second filter membrane 341 , and the top of the shell 342 is connected to the connecting pipe 33 , so that the reagent can flow from the connecting pipe 33 into the shell 342 , thereby acting on the second filter membrane 341 .

[0035] Furthermore, in order to prevent the second filter membrane 341 from falling to the bottom of the tube body 1 due to the impact of the reagent, the bottom of the shell 342 has a semi-open recess B, and the second filter membrane 341 is placed in the recess B. In this way, the installation of the second filter membrane 341 can be completed. After the second filter membrane 341 completes the filtration, the filtered reagent will also be discharged from the open port A of the recess B.

[0036] To further strengthen the installation of the second filter membrane 341 in the recess B, a protrusion 343 is provided on the inner edge of the recess B, and the protrusion 343 supports the second filter membrane 341 , so that the protrusion 343 can bite the second filter membrane 341 , thereby improving the pressure resistance of the second filter membrane 341 .

[0037] In order to achieve rapid filtration of the reagent, a first plug 21 is provided on the tube cover 2, and the first plug 21 will be inserted into the filter funnel 31, wherein the first plug 21 can be made of a flexible material, such as rubber, silicone, etc. In this way, the first plug 21 can be selectively inserted into the filter funnel 31, and the first plug 21 will squeeze the residual air in the filter funnel 31, so that the reagent in the filter funnel 31 is pressurized and accelerated to flow to the first filter part 32. Further, in order to achieve the sealing installation of the tube cover 2 and the opening A, a second plug 22 can be provided on the outer edge of the first plug 21, and the first plug 21 and the second plug 22 are concentric circles, and the diameter of the second plug 22 corresponds to the diameter of the opening A, so as to achieve the sealing installation of the tube cover 2 and the opening A. The thickness of the second plug 22 needs to be smaller than that of the first plug 21, so as not to affect the insertion of the first plug 21 into the filter funnel 31.

[0038] Working principle: Step 1: Complete the assembly of the filter assembly 3, place the first filter membrane 321 at the connecting pipe 33 corresponding to the filter bucket 31 to form the first filter part 32, and then place the second filter membrane 341 from the bottom of the shell 342 to form the second filter part 34.

[0039] Step 2: Install the filter assembly 3, insert the filter assembly 3 assembled in step 1 into the tube body 1 to complete the installation.

[0040] Step 3: Filter, pour the pretreatment reagent from the filter bucket 31, the reagent passes through the first filter membrane 321 to complete the first filtration, then flows into the connecting tube 33, and then flows into the second filter membrane 341 through the connecting tube 33 to complete the second filtration, wherein the second filtered reagent, that is, the target protein, will be collected at the bottom of the tube body 1.

[0041] Step 4: After the collection is completed, take out the filter assembly 3 and then close the tube cover 2.

[0042] The first filter membrane 321 and the second filter membrane 341 mentioned in this patent can be selected according to the experimental object, such as filter membranes with a pore size range of 0.03-0.5μm, 5μm, 0.001-0.01μm, to filter the target pathogens.

[0043] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0044] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A mass spectrometry microbial identification sample protein pretreatment device, characterized by: The invention comprises a tube body, a tube cover, and a filter assembly, wherein the tube cover is detachably mounted on the opening of the tube body; the filter assembly is detachably arranged in the tube body, and the filter assembly comprises a filter bucket, a first filter portion arranged at the bottom of the filter bucket, a connecting pipe arranged at the bottom of the first filter portion, and a second filter portion arranged at the bottom of the connecting pipe; The first filter part and the second filter part are used to filter the pretreatment reagent.

2. A mass spectrometry microorganism identification sample protein pretreatment device according to claim 1, characterized in that: A flexible connecting belt is connected between the tube body and the tube cover.

3. A mass spectrometry microorganism identification sample protein pretreatment device according to claim 1, characterized in that: The tube cover is provided with a first plug, which will be inserted into the filter funnel.

4. The mass spectrometry microorganism identification sample protein pretreatment device according to claim 1, characterized in that: The filter bucket is provided with a limiting ring, and the diameter of the limiting ring is larger than the diameter of the opening.

5. The mass spectrometry microorganism identification sample protein pretreatment device according to claim 1, characterized in that: The first filter part comprises a first filter membrane, and the filter membrane is arranged between the filter bowl and the connecting pipe, wherein the diameter of the filter membrane is larger than the diameter of the connecting pipe.

6. A mass spectrometry microorganism identification sample protein pretreatment device according to claim 5, characterized in that: The inlet of the communicating pipe is provided with a fence.

7. The mass spectrometry microorganism identification sample protein pretreatment device according to claim 1, characterized in that: The second filter portion includes a second filter membrane, and the second filter membrane is located at the outlet of the connecting pipe.

8. The mass spectrometry microorganism identification sample protein pretreatment device according to claim 7, characterized in that: The second filter part comprises a shell for placing the second filter membrane, the top of the shell is communicated with the connecting pipe, and the bottom of the shell has a semi-open recess, and the second filter membrane is placed in the recess.

9. A mass spectrometry microorganism identification sample protein pretreatment device according to claim 8, characterized in that: A protrusion is provided on the inner edge of the recess, and the protrusion supports the second filter membrane.

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