Ovulation device for microinjection

Abstract

The utility model relates to the technical field of cell separation, and particularly discloses an ovulation device for microinjection, which comprises an ovulation table provided with a plurality of linearly arranged grooves; only one egg can be accommodated in the groove; and the bottom of the groove is provided with a drain hole. The ovulation device for microinjection has the advantages that the ovulation device for microinjection comprises an ovulation table, and a plurality of linearly-arranged grooves are formed in the ovulation table; a plurality of egg cells are placed on the ovulation table, and a part of the egg cells sink into the grooves, so that the egg cells can be automatically arranged in a row. The other part of the egg cells which are not sunk into the groove can be easily washed away, and water remaining on the ovulation table in the operation process is drained through the drainage holes. As the number of the grooves is fixed, counting can be directly carried out.

Description

Technical Field

[0001] The utility model relates to the technical field of cell separation equipment, and specifically discloses an ovulation device for microinjection. Background Art

[0002] Microinjection is a method of injecting foreign gene fragments directly into pronuclear embryos or cultured cells using a glass microinjection needle with an extremely fine tip (0.1 to 0.5 μm). The foreign gene is then embedded into the host's chromosomes through possible recombination, deletion, duplication or translocation of the host's genome sequence. This technology is widely used, highly reliable and has good results.

[0003] A Chinese invention patent (authorization announcement number CN105420099A) discloses an efficient microinjection device, which includes a quartz substrate and an injection body, the injection body is fixed on the quartz substrate, the injection body is provided with an input microchannel, an output microchannel and a cell transport microchannel, a microinjection channel and a cell movement control channel are also provided between the input microchannel and the output microchannel on the injection body, the microinjection channel is vertical and vertically connected with the cell transport microchannel, a microinjection needle is fixed on the microinjection channel, there is a gap between the microinjection channel and the microinjection needle, and the gap size is smaller than the cell diameter, a limiting protrusion is provided in the microinjection channel, the cell movement control channel is vertically connected with the microinjection channel, and the cell movement control channel is located above the limiting protrusion. The novel device indirectly controls the cell movement by controlling the movement of the microfluid, so that the cell "himself" hits the microinjection needle, thereby completing the cell microinjection operation. The advantages of the above technical solution are: it can greatly reduce the complexity and cost of the equipment, and is simple to operate. The disadvantages are that it cannot automatically arrange itself in a row when ovulating and does not have an automatic counting function. Utility Model Content

[0004] The technical problem to be solved by the utility model is to provide an ovulation device for microinjection which can automatically ovulate and count.

[0005] In order to solve the above technical problems, the technical solution adopted by the utility model is:

[0006] Provided is an ovulation device for microinjection, comprising an ovulation platform, on which a plurality of linearly arranged grooves are arranged; the groove can only accommodate one egg; and a drainage hole is arranged at the bottom of the groove.

[0007] Furthermore, the groove is a tapered groove whose diameter gradually decreases from top to bottom.

[0008] Furthermore, the diameter of the horizontal cross section of the uppermost end of the groove is 1 to 1.3 mm, and the diameter of the horizontal cross section of the lowermost end is 0.3 to 0.5 mm; the depth of the groove is 0.6 to 0.8 mm.

[0009] Furthermore, the edges of the groove are rounded.

[0010] Furthermore, the ovulation platform is provided with a drainage groove on one side with the groove, the length direction of the drainage groove is perpendicular to the arrangement direction of the groove, and the drainage grooves correspond to the grooves one by one; each drainage groove connects the corresponding groove and the extension of the ovulation platform.

[0011] Furthermore, the bottom surface of the drainage groove is inclined toward the edge of the ovulation platform.

[0012] Furthermore, the table top of the ovulation table is in a "V" shape.

[0013] Furthermore, the included angle of the "V" shape is 179 to 180 degrees.

[0014] The utility model has the beneficial effect of providing an ovulation device for microinjection. When a plurality of oocytes are placed on the ovulation table, a portion of the oocytes will fall into the grooves and automatically line up in a row. The other portion of the oocytes that are not fallen into the grooves can be easily washed away, and the water remaining on the ovulation table during the operation is discharged through the drainage holes. Since the number of grooves is fixed, they can be directly counted. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the structure of the ovulation device for microinjection of the utility model;

[0016] Figure 2 This is a schematic diagram of the ovulation device for microinjection of the utility model loaded with egg cells;

[0017] Figure 3 It is a cross-sectional schematic diagram of the ovulation device for microinjection of the utility model;

[0018] Figure 4 It is a schematic diagram of the groove of the ovulation device for microinjection of the utility model;

[0019] Description of labels:

[0020] 1. Ovulation platform; 11. Groove; 12. Drainage hole; 13. Drainage groove;

[0021] 2. Oocyte. DETAILED DESCRIPTION

[0022] In order to explain the technical content, achieved objectives and effects of the present invention in detail, the following is an explanation in conjunction with the implementation modes and the accompanying drawings.

[0023] See also Figure 1-4 The utility model provides an ovulation device which can automatically ovulate and count eggs. During ovulation, the eggs can be directly arranged in a row and can be automatically counted.

[0024] In order to solve the above technical problems, the technical solution adopted by the utility model is:

[0025] Provided is an ovulation device for microinjection, comprising an ovulation platform 1, on which a plurality of linearly arranged grooves 11 are provided; the groove 11 can only accommodate one egg; and a drainage hole 12 is provided at the bottom of the groove 11.

[0026] From the above description, it can be seen that an ovulation device for microinjection is provided. When in use, multiple oocytes 2 are placed on the ovulation platform 1, and some of the oocytes 2 will fall into the grooves 11 and automatically line up in a row. The other oocytes 2 that are not in the grooves 11 can be easily washed away. During the operation, the water remaining on the ovulation platform 1 is discharged through the drainage holes 12. Since the number of grooves 11 is fixed, they can be directly counted.

[0027] Furthermore, the groove 11 is a tapered groove whose diameter gradually decreases from top to bottom.

[0028] From the above description, it can be seen that the diameters of different egg cells 2 are different. Using conical grooves with different groove depths and different diameters can better accommodate egg cells 2 of different sizes.

[0029] For further information, see Figure 4 The diameter A of the horizontal cross section of the uppermost end of the groove 11 ( Figure 4 ) is 1 to 1.3 mm, and the diameter B of the horizontal cross section at the bottom ( Figure 4 ) is 0.3 to 0.5 mm; the depth C of the groove 11 ( Figure 4 ) is 0.6~0.8 mm.

[0030] It can be seen from the above description that the diameter of the fish eggs is about 1 mm, and the size of the above groove 11 should be adapted to the size of the fish eggs.

[0031] Furthermore, the edge of the groove 11 is rounded.

[0032] It can be seen from the above description that the egg cell 2 trapped in the groove 11 is prevented from being damaged by the edge of the groove 11.

[0033] Furthermore, the ovulation platform 1 is provided with a drainage groove 13 on one side where the groove 11 is provided, the length direction of the drainage groove 13 is perpendicular to the arrangement direction of the groove 11, and the drainage groove 13 corresponds to the groove 11 one by one; each drainage groove 13 connects the corresponding groove 11 and the extension of the ovulation platform 1.

[0034] It can be seen from the above description that the water on the countertop can be drained off the countertop.

[0035] Furthermore, the bottom surface of the drainage groove 13 is inclined toward the edge of the ovulation platform 1 .

[0036] From the above description, it can be seen that the drainage groove 13 is inclined along the outer extension direction of the ovulation platform 1, which is conducive to the automatic discharge of residual water from the ovulation platform 1.

[0037] Furthermore, the table top of the ovulation table 1 is in a "V" shape.

[0038] As can be seen from the above description, when the egg cell 2 is placed on the ovulation table 1, it can slide into the groove 11 along the table surface. And when washing, the egg cell 2 that has not fallen into the groove 11 can slide out of the ovulation table 1 along the angle generated by the two inclined surfaces, making it easier to collect.

[0039] Furthermore, the included angle D of the "V" shape is 179 to 180 degrees.

[0040] From the above description, it can be seen that since the diameter of the egg cell 2 is about 1 mm, the angle of the cross section should not be too large, otherwise the extension of the ovulation platform 1 will be too high, affecting the operation of the staff.

[0041] Embodiment 1:

[0042] Application scenario: This embodiment is an ovulation device that can automatically ovulate and count eggs. During ovulation, the eggs can be directly arranged in a row and can be automatically counted.

[0043] like Figure 1-4 The ovulation device for microinjection shown in the figure comprises an ovulation platform 1, on which a plurality of grooves 11 arranged in a linear manner are provided; the groove 11 can only accommodate one egg, and a drainage hole 12 is provided at the bottom of the groove 11. The groove 11 is a conical groove whose diameter gradually decreases from top to bottom. The ovulation platform 1 is provided with a drainage groove 13 on one side provided with the groove 11, and the length direction of the drainage groove 13 is perpendicular to the arrangement direction of the groove 11, and the drainage groove 13 corresponds to the groove 11 one by one; each drainage groove 13 connects the corresponding groove 11 and the extension of the ovulation platform 1.

[0044] When the ovulation device for microinjection described above is used: multiple ovum cells 2 are placed on the ovulation platform 1, and a part of the ovum cells 2 will fall into the groove 11, and can be automatically arranged in a row. The other part of the ovum cells 2 that are not trapped in the groove 11 can be easily washed away, because the number of the grooves 11 is fixed, and can be directly counted. A drainage hole 12 is provided at the bottom of the groove 11, and the water remaining on the ovulation platform 1 during the operation can be automatically drained. The groove 11 is a tapered groove with a diameter that gradually decreases from top to bottom. There are differences in the diameters of different ovum cells 2. Using a tapered groove, different groove depths have different diameters, which can better accommodate ovum cells 2 of different sizes. At the same time, the above-mentioned drainage groove 13 can drain the water on the table surface.

[0045] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent transformations made using the contents of the specification and drawings of the present invention, or directly or indirectly applied in the relevant technical field, are also included in the patent protection scope of the present invention.

Claims

1. An ovulation device for microinjection, comprising an ovulation platform, characterized in that: The ovulation platform is provided with a plurality of grooves arranged linearly; the groove can only accommodate one egg; a drainage hole is provided at the bottom of the groove; the groove is a conical groove whose diameter gradually decreases from top to bottom; the ovulation platform is provided with a drainage groove on one side provided with the groove, the length direction of the drainage groove is perpendicular to the arrangement direction of the grooves, and the drainage grooves correspond to the grooves one by one; each of the drainage grooves is connected to the corresponding groove and the extension of the ovulation platform.

2. The ovulation device for microinjection according to claim 1, characterized in that: The diameter of the horizontal cross section of the uppermost end of the groove is 1 to 1.3 mm, and the diameter of the horizontal cross section of the lowermost end is 0.3 to 0.5 mm; the depth of the groove is 0.6 to 0.8 mm.

3. The ovulation device for microinjection according to claim 1, characterized in that: The edges of the groove are rounded.

4. The ovulation device for microinjection according to claim 1, characterized in that: The bottom surface of the drainage groove is inclined toward the edge of the ovulation platform.

5. The ovulation device for microinjection according to claim 1, characterized in that: The table top of the ovulation table is in a "V" shape.

6. The ovulation device for microinjection according to claim 5, characterized in that: The included angle of the "V" shape is 179 to 180 degrees.

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