Full-automatic fraction collector for single-cantilever movable liquid chromatography

Abstract

The utility model discloses a full-automatic fraction collector for single-cantilever mobile liquid chromatography, which relates to the technical field of separation and purification of liquid chromatography, and comprises a first bracket, a first driving device, a second driving device, a liquid receiving device, an electric control valve and a controller, the controller controls the first driving device, the second driving device and the electric control valve; and at least one of a laboratory pore plate and a test tube rack is arranged in the liquid receiving device. The liquid receiving device is arranged below the first driving device and the second driving device; the first driving device is arranged on the first bracket and drives the second driving device to do horizontal linear motion; the electric control valve is arranged on the second driving device, and the second driving device drives the electric control valve to do horizontal linear motion; the second driving device and the electric control valve move vertically; the installation height of the electric control valve is adjustable, and the possibility of sputtering and losing can be effectively reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of liquid chromatography separation and purification, and more specifically, to a single-cantilever mobile full-automatic fraction collector for liquid chromatography. Background Art

[0002] At present, the fraction collector is an auxiliary device of the liquid chromatograph, which is mainly used for separation, purification and preparation of pure components. When it is necessary to provide a standard or to further identify a component, the fraction collector is often used to collect the required components at the outlet of the liquid separation column, and the pure component is obtained by removing the solvent in the fraction. The collector can cut manually or automatically according to the signal of the chromatographic peak. The current development trend is to realize the automation of sample injection and fraction collection, so that continuous operation can be carried out and efficiency can be improved.

[0003] Chinese patent CN219348774U provides an automatic fraction collector, which includes: a bracket, an X-direction moving device, a Y-direction moving device, a test tube rack, a collector assembly and a controller, wherein the Y-direction moving device is slidably mounted on the top of the bracket, the X-direction moving device is slidably mounted on the Y-direction moving device, the test tube rack is placed on the bracket, the collector assembly includes a first liquid outlet pipe, a second liquid outlet pipe, a third liquid outlet pipe, a three-way valve 1, a rotary sealing joint, a liquid inlet pipe and a waste liquid cup, the third liquid outlet pipe is connected to the liquid inlet pipe through the rotary sealing joint, the bottom end of the third liquid outlet pipe is connected to the top end of the three-way valve 1, the first liquid outlet pipe and the second liquid outlet pipe are connected to both sides of the three-way valve 1, a waste liquid cup bracket is arranged at the bottom end of the X-direction moving device, the waste liquid cup is placed on the waste liquid cup bracket, the controller is mounted on the side wall of the bracket, and the X-direction moving device, the Y-direction moving device and the three-way valve 1 are all electrically connected to the controller.

[0004] The installation height of the three-way valve in the patent is not adjustable, so that the relative height between the three-way valve and the test tube rack is fixed. When the vertical distance between the test tube mouth and the three-way valve is large, liquid is prone to splashing and loss during dripping, thereby affecting the accuracy and completeness of fraction collection. Utility Model Content

[0005] In view of the problem of the relative height between the valve and the test tube rack being fixed in the prior art, the purpose of the utility model is to provide a single-cantilever mobile fully automatic fraction collector for liquid chromatography, which includes a first bracket, a first drive device, a second drive device, a liquid receiving device, an electric control valve and a controller; the controller is used to control the first drive device, the second drive device and the electric control valve; the liquid receiving device is built with at least one of a laboratory orifice plate and a test tube rack. The liquid receiving device is arranged below the first drive device and the second drive device; the first drive device is installed on the first bracket, and is used to drive the second drive device to perform horizontal linear motion; the electric control valve is installed on the second drive device, and the second drive device is used to drive the electric control valve to perform horizontal linear motion; the moving directions of the second drive device and the electric control valve are perpendicular to each other; the installation height of the electric control valve on the second drive device is adjustable.

[0006] The utility model is further configured as follows: the first driving device includes a first synchronous belt, a first motor and two first pulleys; the two first pulleys are rotatably connected to the first bracket, the first synchronous belt is tensioned on the two first pulleys, and the first motor drives any one of the first pulleys to rotate; the second driving device includes a driving bracket, a second synchronous belt, a second motor, a mounting plate and two second pulleys; the driving bracket is horizontally linearly slidably connected to the first bracket and fixedly connected to the first synchronous belt, the two second pulleys are rotatably connected to the driving bracket, the second synchronous belt is tensioned on the two second pulleys, and the second motor drives any one of the second pulleys to rotate; the mounting plate is horizontally linearly slidably connected to the driving bracket and fixedly connected to the second synchronous belt, the electric control valve is mounted on the mounting plate, and the mounting height of the electric control valve on the mounting plate is adjustable; the first motor and the second motor are controlled by a controller.

[0007] The utility model is further configured as follows: the first driving device also includes a first position sensing component, which is used to detect the position of the second synchronous belt on the first bracket; the second driving device also includes a second position sensing component, which is used to detect the position of the mounting plate on the driving bracket; the first position sensing component and the second position sensing component are connected to the controller signal.

[0008] The utility model is further configured as follows: the laboratory orifice plate and the test tube rack are both detachably connected to the liquid receiving device, the laboratory orifice plate and the test tube rack are interchangeable in the liquid receiving device, and the plane sizes of the laboratory orifice plate and the test tube rack are the same or different.

[0009] The utility model is further configured as follows: it also includes a second bracket, the first bracket is fixedly connected to the second bracket, and the liquid receiving device is detachably connected to the top of the second bracket; a longitudinal through groove is provided on the liquid receiving device, and a storage space connected to the longitudinal through groove is provided below the liquid receiving device.

[0010] The utility model is further configured to include: a liquid leakage detection device, which is used to detect liquid leakage during the fraction collection process; and the liquid leakage detection device is connected to the controller signal.

[0011] The utility model is further configured as follows: it also includes a light strip, which is connected to the controller signal; the lighting mode of the light strip is a combination of an operating mode and a lighting color, the operating mode is any one of a marquee, a breathing light, a periodic flashing, and a constant light, and the light strip has at least one lighting color; in the following three situations, the light strip presents different lighting modes respectively: when the leakage detection device detects leakage, when any one of the first drive device, the second drive device and the electric control valve is working, and when the first drive device, the second drive device and the electric control valve stop working.

[0012] The utility model is further configured to include a photoelectric sensor, which is arranged below the electric control valve and connected to the controller signal, and is used to detect the number of drops of the liquid to be collected that drips from the electric control valve.

[0013] The utility model is further configured as follows: the controller is provided with a data transmission port.

[0014] The utility model is further configured as follows: the electric control valve is a two-position three-way valve, which has a liquid inlet and two liquid outlets, one of which is used to collect liquid fractions, and the other is used to discharge waste liquid.

[0015] In summary, the utility model has the following beneficial effects compared with the prior art: the installation height of the electric control valve of the utility model is adjustable, and it can be compatible with collection containers of different heights, such as laboratory orifice plates and test tubes, so that the vertical distance between the collection container and the starting dripping position of the liquid to be collected is moderate, reducing the possibility of splashing and loss. In addition, the liquid receiving device in the utility model can be compatible with laboratory orifice plates and test tubes at the same time to meet the mixed collection needs, and when the longitudinal groove at the bottom of the liquid receiving device is exposed, the liquid to be collected dripped by the electric control valve can also fall from the longitudinal groove into the large-capacity collection bottle arranged below the longitudinal groove, so that the utility model can be compatible with the collection needs of large-capacity samples. Finally, the utility model also improves the accuracy of the horizontal displacement of the electric control valve through the cooperation of the belt drive and the guide rail slider. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A top view of an embodiment;

[0017] Figure 2 It is a left side view of the embodiment;

[0018] Figure 3 A schematic diagram showing a first synchronous belt in an embodiment;

[0019] Figure 4 A schematic diagram showing a second synchronous belt in an embodiment;

[0020] Figure 5 Schematic diagram of a liquid receiving device in an embodiment.

[0021] In the figure: 1. first bracket; 2. first shell; 21. first synchronous belt; 22. first motor; 23. first pulley; 24. first guide rail; 25. first limit block; 3. second shell; 31. driving bracket; 32. second synchronous belt; 33. second motor; 34. mounting plate; 35. second pulley; 4. liquid receiving device; 41. 96-well plate; 42. 384-well plate; 43. test tube rack; 5. electric control valve; 6. second bracket; 7. photoelectric sensor. DETAILED DESCRIPTION

[0022] The technical solution of the utility model will be clearly described below in conjunction with the accompanying drawings. Obviously, the described embodiments are not all embodiments of the utility model. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0023] It should be noted that the terms "center", "up", "down", "horizontal", "left", "right", "front", "back", "lateral", "longitudinal", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the utility model and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the utility model.

[0024] like Figure 1-5 As shown, it is a schematic diagram of a preferred embodiment of the utility model. This embodiment provides a single-cantilever mobile liquid chromatography full-automatic fraction collector, which includes a first bracket 1, a first drive device, a second drive device, a liquid receiving device 4, an electric control valve 5 and a controller; the controller is used to control the first drive device, the second drive device and the electric control valve 5; the liquid receiving device 4 is built with at least one of a laboratory orifice plate and a test tube rack 43. The liquid receiving device 4 is arranged below the first drive device and the second drive device; the first drive device is installed on the first bracket 1, and is used to drive the second drive device to perform horizontal linear motion; the electric control valve 5 is installed on the second drive device, and the second drive device is used to drive the electric control valve 5 to perform horizontal linear motion; the moving directions of the second drive device and the electric control valve 5 are perpendicular to each other; the installation height of the electric control valve 5 on the second drive device is adjustable, so that the vertical distance between the laboratory orifice plate and the test tube and the starting dripping position of the liquid to be collected is moderate, reducing the possibility of splashing and loss.

[0025] The flow rate of the liquid to be collected can be directly controlled by controlling the opening of the electric control valve 5. At the same time, adjusting the installation height of the electric control valve 5 on the second drive device can also achieve the effect of adjusting the dripping speed or collection rate when the opening adjustment of the electric control valve 5 fails.

[0026] It should be noted that, in order to further increase the height adjustment range of the electric-controlled valve 5 , the installation height of the first driving device relative to the first bracket 1 may also be adjusted.

[0027] The electric control valve 5 in this embodiment is a two-position three-way valve having a liquid inlet and two liquid outlets, one of which is used to collect liquid fractions and the other is used to discharge waste liquid.

[0028] Specifically, the first driving device includes a first synchronous belt 21, a first motor 22 and two first pulleys 23; the two first pulleys 23 are rotatably connected to the first bracket 1, the first synchronous belt 21 is tensioned on the two first pulleys 23, and the first motor 22 drives any one of the first pulleys 23 to rotate; the second driving device includes a driving bracket 31, a second synchronous belt 32, a second motor 33, a mounting plate 34 and two second pulleys 35; the driving bracket 31 is horizontally linearly slidably connected to the first bracket 1 and is fixedly connected to the first synchronous belt 21, the two second pulleys 35 are rotatably connected to the driving bracket 31, the second synchronous belt 32 is tensioned on the two second pulleys 35, and the second motor 33 drives any one of the second pulleys 35 to rotate; the mounting plate 34 is horizontally linearly slidably connected to the driving bracket 31 and is fixedly connected to the second synchronous belt 32, the electric control valve 5 is installed on the mounting plate 34, and the electric control valve 5 is installed on the mounting plate 34 with adjustable height; the first motor 22 and the second motor 33 are controlled by a controller.

[0029] Specifically, the installation height of the electric control valve 5 on the second drive device is adjustable, which can be achieved through the following schemes: Scheme 1, connecting the linear drive device between the electric control valve 5 and the mounting plate 34, and driving the electric control valve 5 to rise and fall relative to the mounting plate 34 through the linear drive device; Scheme 2, vertically slidingly connecting the electric control valve 5 to the mounting plate 34, and fixing the electric control valve 5 at different heights of the mounting plate 34 through fasteners; Scheme 3, directly fixing the electric control valve 5 at different heights of the mounting plate 34 through fasteners.

[0030] Specifically, the first driving device further includes a first guide rail 24 and a first slider, wherein the first guide rail 24 is fixed on the first bracket 1, the first slider is fixed on the driving bracket 31, the first guide rail 24 and the first slider are slidably connected, and the first guide rail 24 and the first slider are used to achieve the effect of horizontal linear sliding connection of the driving bracket 31 on the first bracket 1, so as to assist the first synchronous belt 21 in defining the sliding direction of the driving bracket 31. In this embodiment, two first guide rails 24 are provided, and the first slider is slidably connected between the two first guide rails 24.

[0031] Specifically, the second driving device also includes a second guide rail and a second slider, the second guide rail is fixed on the driving bracket 31, the second slider is fixed on the mounting plate 34, the second guide rail and the second slider are slidably connected, and the second guide rail and the second slider are used to achieve the effect of horizontal linear sliding connection of the mounting plate 34 on the driving bracket 31 to assist the second synchronous belt 32 in limiting the sliding direction of the mounting plate 34.

[0032] Specifically, it also includes a first shell 2 and a second shell 3. The first shell 2 is fixedly connected to the first bracket 1, and the first driving device is located in the first shell 2. The second shell 3 is fixedly connected to the driving bracket 31, and the second driving device is located in the second shell 3.

[0033] Specifically, the first drive device further includes a first position sensor assembly, which is used to detect the position of the second synchronous belt 32 on the first bracket 1. The second drive device further includes a second position sensor assembly, which is used to detect the position of the mounting plate 34 on the drive bracket 31. The first position sensor assembly and the second position sensor assembly are connected to the controller signal to ensure that the stroke of the second drive device and the mounting plate 34 is always within a safe range. In this embodiment, the first position sensor is an encoder built into the first motor 22, and in this embodiment, the second position sensor is an encoder built into the second motor 33.

[0034] Specifically, the first driving device also includes a first limit block 25, two of which are provided, and the two first limit blocks 25 are fixed on the first bracket 1 along the sliding direction of the second driving device to mechanically limit the sliding stroke of the second driving device; the second driving device also includes a second limit block, two of which are provided, and the two second limit blocks are fixed on the driving bracket 31 along the sliding direction of the mounting plate 34 to mechanically limit the sliding stroke of the mounting plate 34.

[0035] Specifically, two first limit blocks 25 are fixed on the first bracket 1 along the sliding direction of the first slider, and the sliding stroke of the first slider is mechanically limited, and the first slider always slides between the two first limit blocks 25. Two second limit blocks are fixed on the driving bracket 31 along the sliding direction of the second slider, and the sliding stroke of the second slider is mechanically limited, and the second slider always slides between the two second limit blocks.

[0036] Specifically, the laboratory orifice plate and the test tube rack 43 are both detachably connected to the liquid receiving device 4, and the laboratory orifice plate and the test tube rack 43 are interchangeable in the liquid receiving device 4 to meet the needs of mixed collection. The plane dimensions of the laboratory orifice plate and the test tube rack 43 are the same or different. When the plane dimensions of the laboratory orifice plate and the test tube rack 43 are the same, the two are easier to interchange. In this embodiment, the plane dimensions of the laboratory orifice plate and the test tube rack 43 are different. The orifice plate for the experiment is a 96-well plate 41 or a 384-well plate 42 with the same plane edge dimensions, and the test tube rack 43 is a standard test tube rack 43. The liquid receiving device 4 in this embodiment can accommodate 2 test tube racks 43, or 4 orifice plates for the experiment, or 1 test tube rack 43 and 2 orifice plates for the experiment at the same time. Figure 1 The test tube rack 43 is temporarily placed above the laboratory well plate for illustration. The test tube rack 43 is not located in the liquid receiving device 4. Please refer to the text description for details.

[0037] This embodiment also includes a second bracket 6, the first bracket 1 is fixedly connected to the second bracket 6, and the liquid receiving device 4 is detachably connected to the top of the second bracket 6 to raise the height of the liquid receiving device 4, so as to facilitate the experimenter to stand and operate. The liquid receiving device 4 is provided with a longitudinal through groove, and a storage space connected to the longitudinal through groove is provided below the liquid receiving device 4. The storage space can be used to place a large-capacity collection bottle. When there is no test tube rack 43 or an experimental orifice plate above the longitudinal through groove, the liquid to be collected dripped from the electric control valve 5 can fall from the longitudinal through groove into the large-capacity collection bottle, so that this embodiment is compatible with the collection requirements of large-capacity samples. In addition, a refrigeration module can also be placed in the storage space to provide a low-temperature environment for samples that require a low-temperature environment.

[0038] This embodiment also includes a liquid leakage detection device, which is used to detect liquid leakage during the fraction collection process; the liquid leakage detection device is connected to the controller signal, and when the liquid leakage detection device detects that there is liquid leakage during the fraction collection process, the controller controls the electric control valve 5 to close. The liquid leakage detection device is a liquid detector or a pressure sensor; the liquid detector is used to detect whether there is liquid around the single-cantilever mobile liquid chromatography full-automatic fraction collector, and if there is liquid, a liquid leakage signal is issued; the pressure sensor is used to detect the pressure at the liquid inlet end of the electric control valve 5, and when the pressure is lower than the set value, a liquid leakage signal is issued.

[0039] This embodiment also includes a light strip, which is connected to the controller signal; the light mode of the light strip is a combination of an operating mode and a light color, the operating mode is any one of a marquee, a breathing light, a periodic flashing, and a constant light, and the light strip has at least one light color; in the following three cases, the light strip presents different light modes respectively: when the leakage detection device detects leakage, when any one of the first drive device, the second drive device and the electric control valve 5 is working, and when the first drive device, the second drive device and the electric control valve 5 stop working.

[0040] When the leakage detection device described in this embodiment detects leakage, the lighting mode of the light strip is red periodic flashing; when any one of the first drive device, the second drive device and the electric control valve 5 is working, the lighting mode of the light strip is blue marquee; when the first drive device, the second drive device and the electric control valve 5 stop working, the lighting mode of the light strip is blue breathing light.

[0041] This embodiment further includes a photoelectric sensor 7 , which is disposed below the electric control valve 5 and connected to the controller signal. The photoelectric sensor 7 is used to detect the number of drops of the liquid to be collected that drips from the electric control valve 5 .

[0042] In this embodiment, the controller is provided with a data transmission port to facilitate the computer to establish communication and realize accurate control of each component through the software on the computer. In this embodiment, the data transmission port includes a BNC connector, a network cable connector and a USB connector.

[0043] In summary, the installation height of the electric control valve 5 of this embodiment is adjustable, and it can be compatible with collection containers of different heights, such as laboratory orifice plates and test tubes, so that the vertical distance between the collection container and the starting dripping position of the liquid to be collected is moderate, reducing the possibility of splashing and loss. At the same time, adjusting the installation height of the electric control valve 5 on the second drive device can also achieve the effect of adjusting the dripping speed or collection rate, without the need for a flow control device, and the cost is low. In addition, the liquid receiving device 4 in this embodiment can be compatible with laboratory orifice plates and test tubes at the same time to meet the mixed collection needs, and when the longitudinal groove at the bottom of the liquid receiving device 4 is exposed, the liquid to be collected dripped by the electric control valve 5 can also fall from the longitudinal groove into the large-capacity collection bottle arranged below the longitudinal groove, so that this embodiment is compatible with the collection needs of large-capacity samples. Finally, this embodiment also improves the accuracy of the horizontal displacement of the electric control valve 5 through the cooperation of the belt drive and the guide rail slider.

[0044] The above description is only the preferred embodiment of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A fully automatic fraction collector for single-cantilever mobile liquid chromatography, characterized in that: The invention comprises a first support (1), a first driving device, a second driving device, a liquid receiving device (4), an electric control valve (5) and a controller; the controller is used to control the first driving device, the second driving device and the electric control valve (5); the liquid receiving device (4) is internally equipped with at least one of a laboratory orifice plate and a test tube rack (43); The liquid receiving device (4) is arranged below the first driving device and the second driving device; the first driving device is mounted on the first bracket (1) and is used to drive the second driving device to perform horizontal linear motion; the electric control valve (5) is mounted on the second driving device and the second driving device is used to drive the electric control valve (5) to perform horizontal linear motion; The moving directions of the second driving device and the electric control valve (5) are perpendicular to each other; and the installation height of the electric control valve (5) on the second driving device is adjustable.

2. The single-cantilever mobile fully automatic fraction collector for liquid chromatography according to claim 1, characterized in that: The first driving device comprises a first synchronous belt (21), a first motor (22) and two first pulleys (23); the two first pulleys (23) are rotatably connected to the first bracket (1), the first synchronous belt (21) is tensioned on the two first pulleys (23), and the first motor (22) drives any one of the first pulleys (23) to rotate; the second driving device comprises a driving bracket (31), a second synchronous belt (32), a second motor (33), a mounting plate (34) and two second pulleys (35); the driving bracket (31) is horizontally linearly slidably connected to the first bracket (1) and is rotatably connected to the first synchronous belt (21). The step belt (21) is fixedly connected, the two second pulleys (35) are rotatably connected to the driving bracket (31), the second synchronous belt (32) is tensioned on the two second pulleys (35), and the second motor (33) drives any one of the second pulleys (35) to rotate; the mounting plate (34) is horizontally linearly slidably connected to the driving bracket (31) and is fixedly connected to the second synchronous belt (32), the electric control valve (5) is mounted on the mounting plate (34), and the electric control valve (5) is mounted on the mounting plate (34) with an adjustable height; the first motor (22) and the second motor (33) are controlled by a controller.

3. The single-cantilever mobile fully automatic fraction collector for liquid chromatography according to claim 2, characterized in that: The first driving device also includes a first position sensing component, which is used to detect the position of the second synchronous belt (32) on the first bracket (1); the second driving device also includes a second position sensing component, which is used to detect the position of the mounting plate (34) on the driving bracket (31); the first position sensing component and the second position sensing component are connected to the controller signal.

4. The single-cantilever mobile fully automatic fraction collector for liquid chromatography according to claim 1, characterized in that: The laboratory orifice plate and the test tube rack (43) are both detachably connected to the liquid receiving device (4), the laboratory orifice plate and the test tube rack (43) are interchangeable in the liquid receiving device (4), and the plane dimensions of the laboratory orifice plate and the test tube rack (43) are the same or different.

5. The single-cantilever mobile fully automatic fraction collector for liquid chromatography according to claim 4, characterized in that: It also comprises a second bracket (6), the first bracket (1) is fixedly connected to the second bracket (6), the liquid receiving device (4) is detachably connected to the top of the second bracket (6); a longitudinal through groove is provided on the liquid receiving device (4), and a storage space connected to the longitudinal through groove is provided below the liquid receiving device (4).

6. The single-cantilever mobile fully automatic fraction collector for liquid chromatography according to claim 1, characterized in that: It also includes a liquid leakage detection device, which is used to detect liquid leakage during the fraction collection process; the liquid leakage detection device is connected to the controller signal.

7. The single-cantilever mobile fully automatic fraction collector for liquid chromatography according to claim 6, characterized in that: It also includes a light strip, which is connected to the controller signal; the light mode of the light strip is a combination of an operating mode and a light color, the operating mode is any one of a marquee, a breathing light, a periodic flashing, and a constant light, and the light strip has at least one light color; In the following three situations, the light strip presents different lighting modes respectively: when the leakage detection device detects leakage, when any one of the first drive device, the second drive device and the electric control valve (5) is working, and when the first drive device, the second drive device and the electric control valve (5) stop working.

8. A single-cantilever mobile fully automatic fraction collector for liquid chromatography according to any one of claims 1 to 7, characterized in that: It also comprises a photoelectric sensor (7), which is arranged below the electric control valve (5) and connected to the controller signal, and is used to detect the number of drops of the liquid to be collected that drips from the electric control valve (5).

9. A single-cantilever mobile fully automatic fraction collector for liquid chromatography according to any one of claims 1 to 7, characterized in that: The controller is provided with a data transmission port.

10. A single-cantilever mobile fully automatic fraction collector for liquid chromatography according to any one of claims 1 to 7, characterized in that: The electric control valve (5) is a two-position three-way valve having a liquid inlet and two liquid outlets, wherein one liquid outlet is used to collect liquid fractions and the other liquid outlet is used to discharge waste liquid.

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