Fixing device of gravity flow protein purification column

Abstract

The utility model discloses a fixing device of a gravity flow protein purification column, and relates to the technical field of molecular biology. A fixing groove of the gravity flow protein purification column is formed in the middle of the fixing main body, two storage grooves are formed in the fixing main body, the two storage grooves are formed in the two sides of the fixing groove respectively, a supporting bottom plate is installed at the bottom of the fixing main body, movable grooves are symmetrically formed in the two upper sides of the fixing main body, and movable placement frames are movably connected into the movable grooves; the middle part of the rear side wall of the fixed main body is movably connected with an adjustable fixing mechanism; the fixing body is supported through the supporting bottom plate, and the supporting stability can be improved; sliding is achieved through the movable containing frame, adjustment of the movable containing frame is convenient to achieve, and meanwhile tools can be placed through the movable containing frame; the fixing position is adjusted through the adjustable fixing mechanism, meanwhile, the fixing main body is conveniently fixed, and when the fixing main body is touched, the phenomena of shaking and toppling are not prone to occurring.

Description

Technical Field

[0001] The utility model belongs to the technical field of molecular biology, and in particular relates to a fixing device for a gravity flow protein purification column. Background Art

[0002] Gravity flow protein purification column is a common method for purifying proteins. Elution is performed under the action of gravity, thereby achieving chromatographic purification. Gravity flow protein purification column can be filled with agarose filler for purifying proteins containing specific tags. When the protein in the protein solution combines with the agarose filler in the gravity flow protein purification column, the eluent is used to elute and remove impurity molecules by gravity, and finally the purified protein is collected.

[0003] The existing gravity flow protein purification column needs to be fixed by a fixing device when in use, and the existing fixing device has the following disadvantages when in use:

[0004] First, the existing fixing device has poor stability when supporting, and when adding liquid to the gravity flow protein purification column, it is easy to cause the fixing device to shake when it touches the fixing device.

[0005] Second, when adding liquid to the gravity flow protein purification column, it is necessary to configure a flask rack to place test tubes, flasks, instruments, etc., which requires searching when adding liquid, wasting time. Utility Model Content

[0006] In order to solve the problems mentioned in the above background technology, the purpose of the utility model is to provide a fixing device for a gravity flow protein purification column.

[0007] The utility model discloses a fixing device for a gravity flow protein purification column, comprising a fixing body, a supporting bottom plate, a movable placement rack and an adjustable fixing mechanism; a fixing groove for the gravity flow protein purification column is provided in the middle of the fixing body, the front side of the fixing groove is open, two storage grooves are provided on the fixing body, the two storage grooves are respectively arranged on both sides of the fixing groove, a supporting bottom plate is installed at the bottom of the fixing body, movable grooves are symmetrically provided on both upper sides of the fixing body, the movable placement racks are movably connected in the movable grooves, and the adjustable fixing mechanism is movably connected in the middle of the rear side wall of the fixing body.

[0008] Preferably, thermal insulation pads are installed on the front and rear end surfaces of the inner side wall of the storage groove and the end surface away from the fixing groove.

[0009] Preferably, drainage holes are provided on both lower sides of the fixed body, the drainage holes are communicated with the bottom of the storage tank, and plugs are installed in the drainage holes.

[0010] Preferably, two plug-in blocks matching the movable grooves are arranged on the lower side of the movable placement rack, a plurality of circular placement grooves and elongated placement grooves are opened on the upper end surface of the movable placement rack, and a pushing handle is installed on the outer side of the upper end surface of the movable placement rack.

[0011] Preferably, a bottom trough body with an open front end and a closed rear end is provided in the middle of the supporting bottom plate, an anti-skid pad is installed at the bottom of the supporting bottom plate, and a plurality of anti-skid grooves are provided at the bottom of the anti-skid pad.

[0012] Preferably, the adjustable fixing mechanism includes a sliding block, a locking bolt, a telescopic rod, and a fixing plate; a sliding groove which passes through from top to bottom is opened in the middle of the rear side wall of the fixing body, the sliding block is movably connected in the sliding groove, a telescopic rod is installed in the middle of the rear end of the sliding block, a fixing plate is installed at the rear end of the telescopic rod, a locking bolt is connected to one side of the rear end of the sliding block, and the head of the locking bolt is in contact with the rear side of the fixing body.

[0013] Preferably, the sliding block is an I-shaped sliding block.

[0014] Preferably, a fixing suction cup is installed on the rear side wall of the fixing plate, and a plurality of fixing holes are opened at the inner edge of the fixing plate.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] 1. The support of the fixed main body is realized by supporting the bottom plate, which can improve the stability of the support.

[0017] Second, a movable placement rack is used to achieve sliding, which facilitates the adjustment of the movable placement rack, and the movable placement rack can also be used to place tools.

[0018] 3. The adjustable fixing position is achieved through an adjustable fixing mechanism, and at the same time, it is easy to fix the fixed body, and it is not easy to shake or tip over when the fixed body is touched. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] For ease of explanation, the present invention is described in detail by the following specific embodiments and drawings.

[0020] Figure 1 It is a schematic diagram of the structure of the utility model;

[0021] Figure 2 It is a structural schematic diagram of a fixed main body in the utility model;

[0022] Figure 3 It is a structural schematic diagram of the movable placement rack in the utility model;

[0023] Figure 4 This is a bottom view of the supporting base plate in the utility model;

[0024] Figure 5 It is a top view of the fixed main body in the utility model.

[0025] In the figure: 1-fixed body; 2-supporting bottom plate; 3-movable placement frame; 4-sliding block; 5-locking bolt; 6-telescopic rod; 7-fixed plate;

[0026] 1-1-fixed slot; 1-2-storage slot; 1-3-movable slot; 1-4-plug;

[0027] 1-21- thermal insulation pad;

[0028] 2-1-bottom tank; 2-2-anti-slip pad; 2-3-anti-slip groove;

[0029] 3-1-plug-in block; 3-2-circular placement slot; 3-3-long placement slot; 3-4-push handle. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical scheme and advantages of the utility model clearer, the utility model is described below by the specific embodiments shown in the accompanying drawings. However, it should be understood that these descriptions are only exemplary and are not intended to limit the scope of the utility model. The structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions that can be implemented in the utility model, so they have no technical substantive significance. Any structural modification, change in proportional relationship or adjustment of size, without affecting the effects that can be produced by the utility model and the purposes that can be achieved, should still fall within the scope of the technical content disclosed by the utility model. In addition, in the following description, the description of known structures and technologies is omitted to avoid unnecessary confusion of the concept of the utility model.

[0031] It should also be noted that, in order to avoid obscuring the present invention due to unnecessary details, only structures and / or processing steps closely related to the scheme according to the present invention are shown in the accompanying drawings, while other details that are not closely related to the present invention are omitted.

[0032] like Figure 1 , Figure 2As shown, this specific embodiment realizes the fixation of the gravity flow protein purification column by a fixed body, and specifically adopts the following technical scheme: it includes a fixed body 1 and a supporting bottom plate 2; a fixing groove 1-1 of the gravity flow protein purification column is provided in the middle of the fixed body 1, and the fixing groove 1-1 can realize the fixation of the gravity flow protein purification column, and the front side of the fixing groove 1-1 is open, and the opening can realize the observation of the gravity flow protein purification column, and two storage grooves 1-2 are provided on the fixed body 1, and the storage groove 1-2 can realize the placement of water or ice cubes. The two storage grooves 1-2 are respectively arranged on both sides of the fixing groove 1-1, and a supporting bottom plate 2 is installed at the bottom of the fixed body 1, and the supporting bottom plate 2 can realize the support of the fixed body 1, as shown in FIG. Figure 5 As shown, the front and rear end surfaces of the inner wall of the storage slot 1-2 and the end surface away from the fixing slot 1-1 are both equipped with a heat insulation pad 1-21, which can achieve heat insulation and facilitate cooling of the fixing slot 1-1. Figure 1 As shown, drainage holes are provided on the two lower sides of the fixed body 1, and the drainage holes are connected to the bottom of the storage tank 1-2. A plug 1-4 is installed in the drainage hole. After the plug 1-4 is removed, drainage is achieved through the drainage hole. Figure 4 As shown, a bottom groove body 2-1 with an open front end and a closed rear end is provided in the middle of the supporting base plate 2, and an anti-skid pad 2-2 is installed at the bottom of the supporting base plate 2. The anti-skid pad 2-2 can achieve anti-skid and improve the stability of the support, and a plurality of anti-skid grooves 2-3 are provided at the bottom of the anti-skid pad 2-2.

[0033] like Figure 1 As shown, in this specific embodiment, a movable placement rack is used to implement the tool, which is convenient for adding liquid to the gravity flow protein purification column. The following technical scheme is specifically adopted: comprising a movable placement rack 3; movable grooves 1-3 are symmetrically opened on the two upper sides of the fixed body 1, and the movable placement rack 3 is movably connected in the movable groove 1-3, and the movable placement rack 3 can move in the movable groove 1-3, such as Figure 3 As shown, two plug-in blocks 3-1 matching with the movable grooves 1-3 are arranged on the lower side of the movable placement rack 3, and the plug-in blocks 3-1 are plugged into the movable grooves 1-3 to facilitate left and right movement. A plurality of circular placement grooves 3-2 and elongated placement grooves 3-3 are provided on the upper end surface of the movable placement rack 3, and the circular placement grooves 3-2 and the elongated placement grooves 3-3 can realize the placement of tools. A pushing handle 3-4 is installed on the outer side of the upper end surface of the movable placement rack 3, and the pushing handle 3-4 can realize the pushing of the movable placement rack 3.

[0034] like Figure 1As shown, in this specific embodiment, an adjustable fixing mechanism is used to fix the fixed body, and the following technical solutions are specifically adopted: including an adjustable fixing mechanism; the middle part of the rear side wall of the fixed body 1 is movably connected with the adjustable fixing mechanism, and the adjustable fixing mechanism can fix the fixed body 1 and can also quickly adjust the position; Figure 5 As shown, the adjustable fixing mechanism includes a sliding block 4, a locking bolt 5, a telescopic rod 6, and a fixing plate 7; a sliding groove that runs through the upper and lower parts is opened in the middle of the rear side wall of the fixed body 1, and the sliding block 4 is movably connected in the sliding groove. The sliding block 4 can slide up and down in the sliding groove to facilitate the adjustment of the fixed position. The sliding block 4 is an I-shaped sliding block, and a telescopic rod 6 is installed in the middle of the rear end of the sliding block 4. A fixing plate 7 is installed at the rear end of the telescopic rod 6. The telescopic rod 6 can increase the telescopic length, and the fixing plate 7 can be realized on the back plate of the laboratory bench. A locking bolt 5 is connected to one side of the rear end of the sliding block 4, and the head of the locking bolt 5 contacts the rear side of the fixed body 1. A fixed suction cup is installed on the rear side wall of the fixing plate 7, and the fixed suction cup can realize rapid adsorption. A number of fixing holes are opened at the inner edge of the fixing plate 7, and bolts can be inserted into the fixing holes and fixed by bolts.

[0035] The working principle of this specific embodiment is as follows: when in use, the gravity flow protein purification column is placed through the fixed groove 1-1 of the fixed body 1, and the opening at the front end of the fixed groove is convenient for observing the gravity flow protein purification column. The support of the fixed body 1 is achieved by the supporting bottom plate 2, and the position of the sliding block 4 is adjusted at the same time, and the extension length of the telescopic rod 6 is adjusted. After the adjustment is completed, the locking of the sliding block 4 is achieved through the locking bolt 5, and finally the fixation is achieved through the fixing plate 7. When conducting an experiment, ice cubes are placed in the storage groove, the movable placement rack 3 is plugged into the movable groove 1-3, and the experimental devices (beakers, test tube racks and other tools) are placed on the movable placement rack 3. During the experiment, the temperature in the storage groove 1-2 is detected by a temperature sensor. When the temperature is too high, the plug 4 is opened to release the water inside, and ice cubes are added again.

[0036] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention.

[0037] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A fixing device for a gravity flow protein purification column, comprising a fixing body (1), a supporting bottom plate (2), a movable placement frame (3), and an adjustable fixing mechanism; a fixing groove (1-1) for a gravity flow protein purification column is provided in the middle of the fixing body (1), the front side of the fixing groove (1-1) is open, and two storage grooves (1-2) are provided on the fixing body (1), and the two storage grooves (1-2) are respectively arranged on both sides of the fixing groove (1-1), characterized in that: A supporting base plate (2) is installed at the bottom of the fixed body (1), movable grooves (1-3) are symmetrically opened on the two upper sides of the fixed body (1), a movable placement frame (3) is movably connected in the movable groove (1-3), and an adjustable fixing mechanism is movably connected in the middle of the rear side wall of the fixed body (1).

2. A fixing device for a gravity flow protein purification column according to claim 1, characterized in that: Heat insulation pads (1-21) are installed on the front and rear end surfaces of the inner side wall of the storage slot (1-2) and the end surface away from the fixing slot (1-1).

3. A fixing device for a gravity flow protein purification column according to claim 1, characterized in that: Drain holes are provided on the two lower sides of the fixed body (1), the drainage holes are connected to the bottom of the storage tank (1-2), and plugs (1-4) are installed in the drainage holes.

4. The fixing device for a gravity flow protein purification column according to claim 1, characterized in that: The lower side of the movable placement rack (3) is provided with two plug-in blocks (3-1) matched with the movable grooves (1-3); the upper end surface of the movable placement rack (3) is provided with a plurality of circular placement grooves (3-2) and elongated placement grooves (3-3); and the outer side of the upper end surface of the movable placement rack (3) is provided with a pushing handle (3-4).

5. The fixing device for a gravity flow protein purification column according to claim 1, characterized in that: A bottom groove body (2-1) with an open front end and a closed rear end is provided in the middle of the supporting bottom plate (2), an anti-skid pad (2-2) is installed at the bottom of the supporting bottom plate (2), and a plurality of anti-skid grooves (2-3) are provided at the bottom of the anti-skid pad (2-2).

6. A fixing device for a gravity flow protein purification column according to claim 1, characterized in that: The adjustable fixing mechanism comprises a sliding block (4), a locking bolt (5), a telescopic rod (6), and a fixing plate (7); a sliding groove which passes through from top to bottom is provided in the middle of the rear side wall of the fixing body (1); the sliding block (4) is movably connected in the sliding groove; a telescopic rod (6) is installed in the middle of the rear end of the sliding block (4); a fixing plate (7) is installed at the rear end of the telescopic rod (6); a locking bolt (5) is connected to one side of the rear end of the sliding block (4); and a head of the locking bolt (5) contacts the rear side of the fixing body (1).

7. A fixing device for a gravity flow protein purification column according to claim 6, characterized in that: The sliding block (4) is an I-shaped sliding block.

8. A fixing device for a gravity flow protein purification column according to claim 6, characterized in that: A fixing suction cup is installed on the rear side wall of the fixing plate (7), and a plurality of fixing holes are provided at the inner edge of the fixing plate (7).

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