Nucleic acid extractor for animal epidemic disease detection

Abstract

The utility model discloses a nucleic acid extraction instrument for animal epidemic disease detection, which comprises a main machine body for animal epidemic disease detection, the main machine body is formed by combining a reagent adding table and a magnetic bead extraction instrument main body, and an automatic feeding and discharging device is arranged in the reagent adding table. The automatic feeding and discharging device comprises a displacement base which is movably arranged on the bottom surface in the reagent adding table through a screw rod transmission mechanism. According to the utility model, the additionally arranged reagent adding table provides a special platform for introducing animal samples and reagents into the reagent box for detection personnel; after introduction, the kit is automatically introduced into the magnetic bead extraction instrument main body through the arranged automatic feeding and discharging device for nucleic acid extraction, and the kit can be automatically reset and guided out after extraction is completed, so that the whole animal nucleic acid extraction operation process is more convenient; and the risk of sample and reagent leakage pollution caused by falling and dumping of the animal nucleic acid detection kit can be effectively avoided, and smooth development of animal epidemic disease detection is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of animal disease detection, in particular to a nucleic acid extractor for animal disease detection. Background Art

[0002] At present, my country's breeding industry is constantly developing and progressing. The breeding method has changed from traditional free-range breeding to large-scale breeding. Animal disease detection is of great significance to the development of the current breeding industry. Carrying out animal disease detection is conducive to timely understanding of the animal's situation and maintaining the animal's growth law. When the disease occurs, it can be studied and examined in time to clarify the cause and root cause, reduce the incidence of the disease, improve the treatment effect, and promote the sustainable development of the breeding industry.

[0003] Moreover, nucleic acid testing is one of the commonly used methods for animal disease detection, and has the advantages of short experimental cycle, high sensitivity, strong specificity, and real-time quantification. At the same time, when extracting nucleic acid from animal samples, it is necessary to introduce the animal samples and the nucleic acid extraction reagents used for animal disease detection into the reagent kit in sequence, and then manually move them to the inside of the nucleic acid extraction instrument, and install the reagent kit, and then manually export it after the test is completed. The whole process is cumbersome on the one hand, and there is a risk of the animal nucleic acid detection kit falling and tipping over, resulting in the leakage and contamination of samples and reagents, which is not conducive to the smooth development of animal disease detection.

[0004] Therefore, it is particularly important to design a nucleic acid extractor for animal disease detection to solve the above-mentioned defects. Utility Model Content

[0005] 1) Technical problems to be solved by the utility model

[0006] In view of the shortcomings of the existing technology, the utility model designs a nucleic acid extraction instrument for animal disease detection, which solves the technical problems in the existing animal disease detection technology, such as the cumbersome manual import and export operations of the test kit and the risk of sample and reagent leakage and contamination due to the falling and tipping of the animal nucleic acid detection kit, which is not conducive to the smooth implementation of animal disease detection.

[0007] 2) Technical solution

[0008] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0009] A nucleic acid extractor for animal disease detection comprises a main body for animal disease detection, the main body being composed of a reagent adding table and a magnetic bead extraction instrument main body, an automatic feeding and discharging device being arranged inside the reagent adding table, the automatic feeding and discharging device comprising a displacement base movably mounted on the bottom surface of the reagent adding table through a screw transmission mechanism, and a reagent reagent rack being movably mounted on the top surface of the displacement base through an electric telescopic rod.

[0010] As a preferred solution of the utility model, the interior of the reagent kit rack is provided with a plurality of groups of box slots, and the reagent kits are embedded and placed inside the box slots. Positioning frames are fixedly installed on the front and rear sides of the reagent kit rack near both ends, and the bottom end of the positioning frame extends to the four sides of the displacement base. The positioning frame is provided with a limiting sliding groove near one side of the displacement base.

[0011] As a preferred solution of the utility model, sliding holes and screw holes are respectively opened through the interior of the displacement base near both ends, and limiting sliders corresponding to the positioning frames are fixedly installed on the front and rear sides of the displacement base near both ends, and the limiting slider and the limiting slide groove are slidably connected.

[0012] As a preferred solution of the utility model, the screw transmission mechanism includes a sliding rod and a screw rod installed through the sliding hole and the screw rod hole. A servo motor is provided at one end of the screw rod, and the servo motor and the screw rod are fixedly connected by a coupling.

[0013] As a preferred solution of the utility model, both ends of the sliding rod are provided with fixing frames, and the end of the screw rod away from the servo motor is provided with a positioning frame, the fixing frame, the positioning frame and the inner wall of the reagent adding platform are all fixedly connected by screws, and the servo motor is fixedly installed inside the reagent adding platform by an L-shaped mounting frame.

[0014] As a preferred solution of the utility model, the reagent adding table is fixedly installed at the front bottom end of the main body of the magnetic bead extraction instrument, and the top surface of the reagent adding table is provided with a rack groove adapted to the reagent kit rack.

[0015] As a preferred solution of the utility model, a control panel is fixedly installed at the top of the front side of the main body of the magnetic bead extraction instrument, a stirring sleeve replacement door is movably installed at the bottom of the control panel, a visual window is provided on the stirring sleeve replacement door, and a magnetic rod and a stirring sleeve mechanism are movably installed near the stirring sleeve replacement door inside the main body of the magnetic bead extraction instrument through a driving mechanism.

[0016] 3) Beneficial effects

[0017] Compared with the prior art, the beneficial effects of the utility model are:

[0018] The utility model provides a dedicated platform for introducing animal samples and reagents into the test kit for test personnel through an additional reagent adding table. After the introduction, the test kit is automatically introduced into the interior of the magnetic bead extraction instrument body for nucleic acid extraction through an automatic loading and unloading device. After the extraction is completed, it can be automatically reset and exported, thereby making the entire animal nucleic acid extraction operation process more convenient, and can effectively avoid the risk of sample and reagent leakage and contamination caused by the animal nucleic acid detection kit falling and tipping over, thereby ensuring the smooth development of animal disease detection, thereby effectively solving the problems raised in the above-mentioned background technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall three-dimensional external structure of the utility model;

[0020] Figure 2 It is a schematic diagram of the three-dimensional structure of the automatic feeding and discharging device in the utility model;

[0021] Figure 3 It is a three-dimensional enlarged structural schematic diagram of the reagent box rack in the utility model;

[0022] Figure 4 It is a three-dimensional enlarged structural schematic diagram of the displacement base in the utility model;

[0023] Figure 5 It is a three-dimensional enlarged structural diagram of the screw transmission mechanism in the utility model;

[0024] In the figure: 1. main body; 2. reagent adding table; 3. main body of magnetic bead extraction instrument; 31. control panel; 32. stirring sleeve replacement door; 321. visual window; 33. magnetic rod and stirring sleeve mechanism; 4. automatic feeding and discharging device; 41. screw transmission mechanism; 411. slide rod; 4111. fixed frame; 412. screw rod; 4121. positioning frame; 413. servo motor; 4131. L-shaped mounting frame; 42. displacement base; 421. slide hole; 422. screw rod hole; 423. limit slider; 43. electric telescopic rod; 44. reagent kit rack; 441. box slot; 442. reagent kit; 443. positioning stand; 4431. limit slide slot. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0026] Example:

[0027] The utility model provides a nucleic acid extraction instrument for animal disease detection, which solves the technical problems in the existing animal disease detection technology that the manual import and export operation of the reagent kit is troublesome, and there is a risk of sample and reagent leakage and contamination caused by the animal nucleic acid detection kit falling and tipping, which is not conducive to the smooth development of animal disease detection;

[0028] See also Figure 1-Figure 5 The utility model provides a technical solution: a nucleic acid extractor for animal disease detection, comprising a main body 1 for animal disease detection, the main body 1 is composed of a reagent adding table 2 and a magnetic bead extraction instrument main body 3, the reagent adding table 2 is fixedly installed at the bottom end of the front side of the magnetic bead extraction instrument main body 3, a control panel 31 is fixedly installed at the top end of the front side of the magnetic bead extraction instrument main body 3, a stirring sleeve replacement door 32 is movably installed at the bottom of the control panel 31, a visual window 321 is provided on the stirring sleeve replacement door 32, and the interior of the magnetic bead extraction instrument main body 3 is close to the stirring sleeve replacement door A magnetic rod and a stirring sleeve mechanism 33 are movably installed at 32 through a driving mechanism. Before extraction, the stirring sleeve can be embedded and installed inside the main body 3 of the magnetic bead extraction instrument through a stirring sleeve replacement door 32. During extraction, the nucleic acid molecules released from the animal sample are specifically adsorbed to the surface of the magnetic beads, and impurities such as proteins are not adsorbed but remain in the solution. Under the action of the driving mechanism, the magnetic rod moves the magnetic beads carrying nucleic acids to different reagent tanks through the stirring sleeve. By repeatedly stirring and mixing the liquid quickly, after steps such as cell lysis, nucleic acid adsorption, washing and elution, pure nucleic acids are finally obtained.

[0029] An automatic feeding and discharging device 4 is provided inside the reagent adding station 2.

[0030] Among them, the automatic feeding and discharging device 4 includes a displacement base 42 movably installed on the bottom surface of the reagent adding platform 2 through a screw transmission mechanism 41, and the interior of the displacement base 42 is respectively penetrated by a sliding hole 421 and a screw hole 422 near both ends. The screw transmission mechanism 41 includes a sliding rod 411 and a screw rod 412 penetrated and installed in the sliding hole 421 and the screw hole 422. A servo motor 413 is arranged at one end of the screw rod 412, and the servo motor 413 and the screw rod 412 are fixedly connected by a coupling. A reagent kit rack 44 is movably installed on the top surface of the displacement base 42 through an electric telescopic rod 43, and a plurality of groups of box slots 441 are opened inside the reagent kit rack 44, and a reagent kit 442 is embedded and placed inside the box slot 441. A rack slot compatible with the reagent kit rack 44 is opened on the top surface of the reagent adding platform 2. When injecting samples, the reagent kit rack 44 is located inside the rack slot, and the required reagent kit 442 for animal nucleic acid detection can be embedded in To the inside of the box slot 441, and introduce the animal sample and the required reagents into the reagent box slot on the top of the reagent adding table 2. After the sample is injected, the electric telescopic rod 43 shrinks downward, driving the reagent box rack 44 downward until the reagent box 442 enters the inside of the reagent adding table 2. At this time, the servo motor 413 works, and under the action of the screw rod 412, the displacement base 42 moves from one end to the other end inside the reagent adding table 2, thereby driving the reagent box 442 to move to the bottom of the magnetic rod and the stirring sleeve mechanism 33, and then the animal nucleic acid is extracted. After the extraction, the automatic feeding and discharging device 4 automatically resets, so that the reagent box 442 after the animal nucleic acid extraction is automatically exported to the top surface of the reagent adding table 2, thereby making the animal nucleic acid extraction operation process more convenient, and can effectively avoid the risk of sample and reagent leakage and contamination caused by the animal nucleic acid detection reagent box falling and tipping, thereby ensuring the smooth development of animal disease detection;

[0031] In addition, in this embodiment, please refer to Figure 2 , Figure 3 and Figure 4 , positioning frames 443 are fixedly installed on both sides of the reagent box rack 44 near both ends, the bottom of the positioning frames 443 extends to the four sides of the displacement base 42, and a limiting slide groove 4431 is provided on one side of the positioning frames 443 near the displacement base 42, and limiting sliders 423 corresponding to the positioning frames 443 are fixedly installed on both sides of the displacement base 42 near both ends, and the limiting slider 423 is slidably connected with the limiting slide groove 4431, and the positioning frames 443, the limiting slide groove 4431 and the limiting slider 423 can make the lifting process of the reagent box rack 44 stable and reliable, thereby facilitating the automatic import and export of the reagent box 442 and the stability during the extraction process;

[0032] In addition, in this embodiment, please refer to Figure 5, both ends of the slide bar 411 are provided with a fixing frame 4111, and the end of the screw rod 412 away from the servo motor 413 is provided with a positioning frame 4121, the fixing frame 4111, the positioning frame 4121 and the inner wall of the reagent adding station 2 are fixedly connected by screws, and the servo motor 413 is fixedly installed inside the reagent adding station 2 through an L-shaped mounting frame 4131, and the fixing frame 4111, the positioning frame 4121 and the L-shaped mounting frame 4131 are provided to facilitate the installation of the screw rod transmission mechanism 41 inside the reagent adding station 2;

[0033] It should be noted that the magnetic bead nucleic acid extractor and its internal driving mechanism are existing technologies and will not be described in detail here.

[0034] The working process of the utility model is as follows: during sample injection, the reagent kit rack 44 is located inside the rack slot, and the reagent kit 442 required for animal nucleic acid detection can be embedded into the inside of the box slot 441, and the animal sample and the required reagents are introduced into the reagent kit slot on the top of the reagent adding station 2. After the sample injection is completed, the electric telescopic rod 43 contracts downward, driving the reagent kit rack 44 downward until the reagent kit 442 enters the inside of the reagent adding station 2. At this time, the servo motor 413 works, and under the action of the screw rod 412, the displacement base 42 moves from one end to the other end inside the reagent adding station 2, thereby driving the reagent kit 442 to move to the bottom of the magnetic rod and the stirring sleeve mechanism 33, and then the animal nucleic acid is extracted. During the extraction, free nucleic acids in the animal sample are The nucleic acid molecules are specifically adsorbed onto the surface of the magnetic beads, while impurities such as proteins are not adsorbed and remain in the solution. Under the action of the driving mechanism, the magnetic rod moves the magnetic beads carrying the nucleic acid to different reagent tanks through the stirring sleeve. By repeatedly stirring and mixing the liquid quickly, after cell lysis, nucleic acid adsorption, washing and elution, pure nucleic acid is finally obtained. After extraction, the automatic feeding and discharging device 4 automatically resets, so that the reagent kit 442 after the animal nucleic acid extraction is automatically exported to the top surface of the reagent adding table 2, thereby making the animal nucleic acid extraction operation process more convenient, and can effectively avoid the risk of sample and reagent leakage and contamination caused by the falling and dumping of the animal nucleic acid detection kit, thereby ensuring the smooth implementation of animal disease detection.

[0035] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid extraction instrument for animal disease detection, comprising a main body (1) for animal disease detection, characterized in that: The main body (1) is composed of a reagent adding platform (2) and a magnetic bead extraction instrument main body (3). An automatic loading and unloading device (4) is arranged inside the reagent adding platform (2). The automatic loading and unloading device (4) includes a displacement base (42) movably mounted on the bottom surface of the reagent adding platform (2) through a screw transmission mechanism (41). The top surface of the displacement base (42) is movably mounted with a reagent reagent rack (44) through an electric telescopic rod (43).

2. The nucleic acid extraction instrument for animal disease detection according to claim 1, characterized in that: The reagent box rack (44) is provided with a plurality of groups of box slots (441) inside, and the reagent box (442) is embedded and placed inside the box slots (441). Positioning frames (443) are fixedly installed near both ends of the front and rear sides of the reagent box rack (44). The bottom end of the positioning frame (443) extends to the periphery of the displacement base (42), and a limiting sliding groove (4431) is provided on a side of the positioning frame (443) close to the displacement base (42).

3. The nucleic acid extraction instrument for animal disease detection according to claim 1, characterized in that: The displacement base (42) is provided with a sliding hole (421) and a screw rod hole (422) respectively near both ends inside the displacement base (42), and a limiting sliding block (423) corresponding to the positioning stand (443) is fixedly installed near both ends on the front and rear sides of the displacement base (42), and the limiting sliding block (423) is slidably connected to the limiting sliding groove (4431).

4. The nucleic acid extraction instrument for animal disease detection according to claim 1, characterized in that: The screw transmission mechanism (41) comprises a sliding rod (411) and a screw rod (412) which are installed through a sliding hole (421) and a screw rod hole (422); a servo motor (413) is arranged at one end of the screw rod (412); and the servo motor (413) and the screw rod (412) are fixedly connected via a coupling.

5. The nucleic acid extraction instrument for animal disease detection according to claim 4, characterized in that: Both ends of the slide bar (411) are provided with a fixing frame (4111), and the end of the screw rod (412) away from the servo motor (413) is provided with a positioning frame (4121), and the fixing frame (4111), the positioning frame (4121) and the inner wall of the reagent adding platform (2) are all fixedly connected by screws, and the servo motor (413) is fixedly installed inside the reagent adding platform (2) by an L-shaped mounting frame (4131).

6. The nucleic acid extraction instrument for animal disease detection according to claim 1, characterized in that: The reagent adding platform (2) is fixedly mounted at the front bottom end of the magnetic bead extraction instrument body (3), and a rack groove matching the reagent reagent rack (44) is provided on the top surface of the reagent adding platform (2).

7. The nucleic acid extraction instrument for animal disease detection according to claim 6, characterized in that: A control panel (31) is fixedly mounted at the top of the front side of the main body (3) of the magnetic bead extraction instrument, a stirring sleeve replacement door (32) is movably mounted at the bottom of the control panel (31), a visual window (321) is provided on the stirring sleeve replacement door (32), and a magnetic rod and a stirring sleeve mechanism (33) are movably mounted inside the main body (3) of the magnetic bead extraction instrument near the stirring sleeve replacement door (32) via a driving mechanism.

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