Culture solution mixing device for culturing mesenchymal stem cells

Abstract

The utility model relates to a mixing device, belongs to the technical field of cell culture devices, and particularly relates to a culture solution mixing device for culturing mesenchymal stem cells. The hydraulic cylinder is fixedly mounted on the top end wall surface of the culture tank, the output end of the hydraulic cylinder is fixedly connected with a connecting rod, a mounting plate is fixedly mounted on the bottom end wall surface of the connecting rod, a threaded rod is fixedly mounted on the inner bottom end wall surface of the culture tank, and a stirring rod is mounted on the side wall of the threaded rod in a threaded manner; the hydraulic cylinder is started to drive the stirring rod to move downwards, the stirring rod rotates clockwise in the thread direction of the threaded rod when moving downwards, then the stirring blades are driven to stir a culture solution, when the hydraulic cylinder drives the stirring rod to move upwards, the stirring rod rotates upwards anticlockwise, multi-layer mixing and stirring of the culture solution are achieved, the mixing speed is increased, and the stirring efficiency is improved. The problem that an existing culture solution mixing device for culturing the mesenchymal stem cells is low in culture solution mixing efficiency is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture devices, in particular to a culture fluid mixing device for culturing mesenchymal stem cells. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions.

[0003] The Chinese patent with publication number CN115992040A relates to the technical field of cell culture devices, and specifically to a mesenchymal stem cell culture device, including multiple support components, a culture tank, a mixing component, a first motor, a connecting shaft, a connecting rod and two cleaning brushes; when culturing the mesenchymal stem cells, the culture fluid and the mesenchymal stem cells are placed in the culture tank, and the culture fluid and the mesenchymal stem cells are stirred and mixed by the mixing component, so as to culture the mesenchymal stem cells; when it is necessary to clean the suspended cells attached to the inner wall of the culture tank, clean water is first introduced into the culture tank, and then the first motor is turned on to drive the connecting shaft to rotate, and the connecting shaft drives the two cleaning brushes to rotate through the connecting rod, so as to clean the suspended cells attached to the inner wall of the culture tank, and no manual cleaning is required, which saves time and physical strength of the staff.

[0004] The microbial culture device of the above technical solution uses a motor to drive two rotating shafts to rotate, thereby driving multiple stirring blades to stir the culture solution. However, during the stirring process, the stirring blades always rotate in the same direction, and the microbial culture solution is difficult to mix evenly, and the mixing efficiency is low. Utility Model Content

[0005] In order to solve the above technical problems, the utility model proposes a culture medium mixing device for mesenchymal stem cell culture. The hydraulic cylinder is started to drive the stirring rod to move downward. When the stirring rod moves downward, it rotates clockwise along the thread direction of the threaded rod, thereby driving the stirring blade to stir the culture medium. When the hydraulic cylinder drives the stirring rod to move upward, the stirring rod rotates counterclockwise upward, thereby achieving multi-level mixing and stirring of the culture medium and improving the mixing rate.

[0006] The technical solution to achieve the purpose of the utility model is: a culture medium mixing device for culturing mesenchymal stem cells, comprising a culture tank, the top of the side wall of the culture tank is fixedly connected to a feed pipe, the bottom wall of the culture tank is fixedly connected to a discharge pipe, and also includes;

[0007] A hydraulic cylinder is fixedly installed on the top wall of a culture tank, the output end of the hydraulic cylinder is fixedly connected to a connecting rod, the bottom wall of the connecting rod is fixedly installed with a mounting plate, the top side wall of the mounting plate is provided with a groove, the inner side wall of the groove is fixedly installed with a first bearing, the inner bottom wall of the culture tank is fixedly installed with a threaded rod, the side wall of the threaded rod is threadedly installed with a stirring rod, the side wall of the stirring rod is equidistantly installed with a plurality of stirring blades, the first bearing is sleeved on the top of the side wall of the stirring rod, and the mounting plate and the stirring rod are connected through the first bearing.

[0008] In some embodiments, a second bearing is sleeved on the bottom end of the side wall of the stirring rod, a movable plate is fixedly mounted on the outer side wall of the second bearing, and a plurality of open grooves are equidistantly formed on the top wall of the movable plate.

[0009] In some embodiments, an annular plate is fixedly mounted on the top wall of the movable plate, and the outer side walls of the movable plate and the annular plate fit with the inner side walls of the culture tank.

[0010] In some embodiments, a plurality of fixing rods are fixedly mounted on the top wall of the annular plate, and the top wall of the annular plate is connected to the inner top wall of the culture tank via the fixing rods.

[0011] In some embodiments, the inner side wall of the annular plate is provided with two arcuate grooves, and the two arcuate grooves are longitudinally distributed in an axisymmetric manner.

[0012] In certain embodiments, a first one-way valve is fixedly mounted on the side wall of the feed pipe, and a second one-way valve is fixedly mounted on the side wall of the discharge pipe.

[0013] Compared with the prior art, the present invention has the following significant advantages:

[0014] First, the utility model starts the hydraulic cylinder, and the hydraulic cylinder drives the connecting rod to move downward, and the movement of the connecting rod drives the mounting plate to move. When the mounting plate moves downward, the stirring rod will be driven to move downward. Since the stirring rod is connected to the threaded rod by threads, when the stirring rod moves downward, the stirring rod will rotate clockwise around the threaded rod, so that the stirring rod drives multiple stirring blades to rotate, and stir the culture medium. When the hydraulic cylinder drives the mounting plate to move upward, the stirring rod will rotate counterclockwise upward along the thread direction of the threaded rod, and the culture medium can be stirred in different directions to achieve multi-level mixing and stirring, thereby improving the mixing rate. The problem of low culture medium mixing efficiency in the existing culture medium mixing device for culturing mesenchymal stem cells is solved.

[0015] Secondly, in the process of the hydraulic cylinder driving the stirring rod to move downward, the movable plate will move downward together with the stirring rod. At this time, the culture liquid under the movable plate will gush out from the open groove, flow toward the direction of the stirring blade through the shape of the arc groove under the inner side wall of the annular plate, and then be fully stirred by the stirring blade. When the movable plate subsequently moves upward along with the stirring rod, the culture liquid above the movable plate will flow into the open groove along the arc groove above the inner side wall of the annular plate. Through the arrangement of the movable plate and the annular plate, the culture liquid inside the culture tank can be fully stirred, thereby improving the practicability of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The utility model is further explained below in conjunction with the accompanying drawings and embodiments:

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 It is a cross-sectional view of the utility model;

[0019] Figure 3 This is a structural schematic diagram of the moving process of the movable plate of the utility model;

[0020] Figure 4 It is a cross-sectional view of the annular plate of the utility model;

[0021] Figure 5 It is an enlarged view of point A of the present utility model.

[0022] Description of reference numerals:

[0023] 1. Culture tank; 11. Feed pipe; 12. Discharge pipe; 13. First one-way valve; 14. Second one-way valve; 2. Hydraulic cylinder; 21. Connecting rod; 22. Mounting plate; 23. First bearing; 24. Stirring rod; 241. Stirring blade; 3. Moving plate; 31. Opening groove; 32. Second bearing; 4. Ring plate; 5. Threaded rod; 6. Fixed rod. DETAILED DESCRIPTION

[0024] The utility model is described in detail below, and the technical solutions in the embodiments of the utility model are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] The utility model provides a culture medium mixing device for culturing mesenchymal stem cells through improvement. The technical solution of the utility model is:

[0026] like Figure 1 - Figure 5 As shown, a culture medium mixing device for culturing mesenchymal stem cells comprises a culture tank 1, a feed pipe 11 is fixedly connected to the top of the side wall of the culture tank 1, a discharge pipe 12 is fixedly connected to the bottom wall of the culture tank 1, and a hydraulic cylinder 2 is also included; the hydraulic cylinder 2 is fixedly installed on the top wall of the culture tank 1, a connecting rod 21 is fixedly connected to the output end of the hydraulic cylinder 2, a mounting plate 22 is fixedly installed on the bottom wall of the connecting rod 21, a groove is provided on the top side wall of the mounting plate 22, a first bearing 23 is fixedly installed on the inner side wall of the groove, a threaded rod 5 is fixedly installed on the inner bottom wall of the culture tank 1, a stirring rod 24 is threadedly installed on the side wall of the threaded rod 5, the thread angle of the threaded rod 5 is preferably between 45° and 60°, so that the stirring rod 24 can spirally rotate along the thread direction of the threaded rod 5, the stirring rod 24 can rotate along the thread direction of the threaded rod 5 by setting the first bearing 23, and a plurality of equidistantly installed side walls of the stirring rod 24 A stirring blade 241 is provided, and a first bearing 23 is sleeved on the top of the side wall of the stirring rod 24. The mounting plate 22 and the stirring rod 24 are connected through the first bearing 23. The culture solution to be stirred and mixed is injected into the culture tank 1 from the feed pipe 11, and the hydraulic cylinder 2 is started. The hydraulic cylinder 2 drives the connecting rod 21 to move downward, and the movement of the connecting rod 21 drives the mounting plate 22 to move. When the mounting plate 22 moves downward, the stirring rod 24 is driven to move downward. Since the stirring rod 24 is connected to the threaded rod 5 by threads, the stirring rod 24 rotates clockwise around the threaded rod 5 during the downward movement of the stirring rod 24, so that the stirring rod 24 drives multiple stirring blades 241 to rotate and stir the culture solution. When the hydraulic cylinder 2 drives the mounting plate 22 to move upward, the stirring rod 24 rotates counterclockwise upward along the thread direction of the threaded rod 5. By stirring the culture solution in different directions, multi-level mixing and stirring are achieved, thereby improving the mixing rate.

[0027] like Figure 2 — Figure 5As shown, in one embodiment, the bottom end of the side wall of the stirring rod 24 is sleeved with a second bearing 32, the outer side wall of the second bearing 32 is fixedly installed with a moving plate 3, the top wall of the moving plate 3 is equidistantly provided with a plurality of open grooves 31, the top wall of the moving plate 3 is fixedly installed with an annular plate 4, the outer side walls of the moving plate 3 and the annular plate 4 are in contact with the inner side walls of the culture tank 1, a plurality of fixing rods 6 are fixedly installed on the top wall of the annular plate 4, the top wall of the annular plate 4 is connected to the inner top wall of the culture tank 1 through the fixing rods 6, the fixing rods 6 and the second bearing 32 are arranged to prevent the annular plate 4 from rotating with the rotation of the stirring rod 24, the inner side wall of the annular plate 4 is provided with two arc grooves, the two The arc grooves are axially symmetrically distributed longitudinally; when the hydraulic cylinder 2 drives the stirring rod 24 to move downward, the moving plate 3 will move downward with the stirring rod 24. At this time, the culture liquid under the moving plate 3 will gush out from the open groove 31, flow toward the direction of the stirring blade 241 through the shape of the arc groove under the inner side wall of the annular plate 4, and then be fully stirred by the stirring blade 241. When the moving plate 3 subsequently moves upward with the stirring rod 24, the culture liquid above the moving plate 3 will flow into the open groove 31 along the arc groove above the inner side wall of the annular plate 4. The arrangement of the moving plate 3 and the annular plate 4 allows the culture liquid inside the culture tank 1 to be fully stirred, thereby improving the practicability of the device.

[0028] like Figure 1 - Figure 4 As shown, in one embodiment, a first one-way valve 13 is fixedly installed on the side wall of the feed pipe 11, and the culture solution from outside enters the culture tank 1 through the first one-way valve 13, thereby preventing the culture solution in the culture tank 1 from flowing out of the feed pipe 11. A second one-way valve 14 is fixedly installed on the side wall of the discharge pipe 12, and the second one-way valve 14 prevents the culture solution in the culture tank 1 from flowing out of the discharge pipe 12 and causing waste.

[0029] The specific working method is: inject the culture solution that needs to be stirred and mixed from the feed pipe 11 into the culture tank 1, start the hydraulic cylinder 2, the hydraulic cylinder 2 drives the connecting rod 21 to move downward, the movement of the connecting rod 21 drives the mounting plate 22 to move, and in the process of the mounting plate 22 moving downward, it will drive the stirring rod 24 to move downward. Since the stirring rod 24 is connected to the threaded rod 5 by threads, when the stirring rod 24 moves downward, the moving plate 3 will move downward with the stirring rod 24. At this time, the culture solution under the moving plate 3 will gush out from the opening groove 31, and flow to the stirring blade through the shape of the arc groove under the inner side wall of the annular plate 4. The stirring rod 24 rotates clockwise around the threaded rod 5 in the direction of the blade 241, so that the stirring rod 24 drives the multiple stirring blades 241 to rotate and stir the culture solution. When the hydraulic cylinder 2 drives the mounting plate 22 to move upward, the stirring rod 24 rotates counterclockwise upward along the thread direction of the threaded rod 5, and the movable plate 3 also moves upward with the stirring rod 24. At this time, the culture solution above the movable plate 3 flows into the open groove 31 along the arc groove above the inner side wall of the annular plate 4. Repeat this operation for stirring, and finally flow out from the discharge pipe 12. By stirring the culture solution in different directions, multi-level mixing and stirring are achieved, thereby improving the mixing rate.

[0030] The technical means disclosed in the utility model are not limited to the technical means disclosed in the above technical means, but also include technical solutions composed of equivalent replacement of the above technical features. Matters not covered in the utility model belong to the common knowledge of those skilled in the art.

Claims

1. A culture medium mixing device for culturing mesenchymal stem cells, comprising a culture tank (1), wherein a feed pipe (11) is fixedly connected to the top of the side wall of the culture tank (1), and a discharge pipe (12) is fixedly connected to the bottom wall of the culture tank (1), characterized in that: Also includes; A hydraulic cylinder (2) is fixedly mounted on the top wall of a culture tank (1); a connecting rod (21) is fixedly connected to the output end of the hydraulic cylinder (2); a mounting plate (22) is fixedly mounted on the bottom wall of the connecting rod (21); a groove is provided on the top side wall of the mounting plate (22); a first bearing (23) is fixedly mounted on the inner side wall of the groove; a threaded rod (5) is fixedly mounted on the inner bottom wall of the culture tank (1); a stirring rod (24) is threadedly mounted on the side wall of the threaded rod (5); a plurality of stirring blades (241) are equidistantly mounted on the side wall of the stirring rod (24); the first bearing (23) is sleeved on the top of the side wall of the stirring rod (24); and the mounting plate (22) and the stirring rod (24) are connected via the first bearing (23).

2. The culture medium mixing device for culturing mesenchymal stem cells according to claim 1, characterized in that: A second bearing (32) is sleeved on the bottom end of the side wall of the stirring rod (24), a movable plate (3) is fixedly mounted on the outer side wall of the second bearing (32), and a plurality of opening grooves (31) are equidistantly formed on the top wall of the movable plate (3).

3. The culture medium mixing device for culturing mesenchymal stem cells according to claim 2, characterized in that: An annular plate (4) is fixedly mounted on the top wall of the movable plate (3), and the outer side walls of the movable plate (3) and the annular plate (4) are in contact with the inner side walls of the culture tank (1).

4. The culture medium mixing device for culturing mesenchymal stem cells according to claim 3, characterized in that: A plurality of fixing rods (6) are fixedly mounted on the top wall surface of the annular plate (4), and the top wall surface of the annular plate (4) is connected to the inner top wall surface of the culture tank (1) via the fixing rods (6).

5. The culture medium mixing device for culturing mesenchymal stem cells according to claim 3, characterized in that: The inner side wall of the annular plate (4) is provided with two arc-shaped grooves, which are longitudinally distributed in an axisymmetric manner.

6. The culture medium mixing device for culturing mesenchymal stem cells according to claim 1, characterized in that: A first one-way valve (13) is fixedly mounted on the side wall of the feed pipe (11), and a second one-way valve (14) is fixedly mounted on the side wall of the discharge pipe (12).

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