Full-automatic brucellosis antibody detection workstation

Abstract

The utility model provides a full-automatic brucellosis antibody detection workstation. The full-automatic brucellosis antibody detection workstation comprises a cabinet body, a pipettor, a sample frame, a sample tube, a suction head box, an oscillation module, an experiment board, a waste liquid tank, a photographing unit, a disinfectant tank and a reagent tank, the suction head box, the waste liquid tank, the disinfectant tank and the reagent tank are located in the middle, and the suction head box is used for providing suction head consumables; the waste liquid tank is used for recycling waste liquid and sucker consumables, the disinfectant tank is used for providing disinfectant, and the reagent tank is used for providing reagents. According to the multi-region physical sample loading device disclosed by the utility model, sample loading, accurate pipetting, liquid transferring, pore plate filling, reagent loading, uniform mixing and oscillation, reagent extraction, detection system construction and sample photographing can be integrated, and multi-region physical division can be carried out according to the sample loading requirements of an experiment sequence, time and each step; cross contamination of samples is avoided, the high-throughput sample treatment requirement is met, full oscillation and uniform mixing can be realized, the accuracy of a detection result is improved, and the detection efficiency is also greatly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of virus detection, in particular to a full-automatic brucellosis antibody detection workstation. Background Art

[0002] Brucellosis is an infectious disease caused by infection with Brucella bacteria and is common to both humans and animals. The tiger red plate agglutination test is also called the Bancroftan red plate agglutination test. The antigen used is an acidic (PH3.6-3.9) colored antigen. When the antigen reacts with the serum being tested, it can inhibit the agglutination activity of the IgM class antibodies in the serum, and the antibodies tested are IgG class, thus improving the specificity of the reaction. When being tested, 25ul of serum and antigen need to be drawn separately and added to both sides of the grid in the glass plate, and then the serum and antigen are quickly mixed, and the results are observed after standing for 4 minutes. When the standard negative serum does not agglutinate and the standard positive serum agglutinates, the test is established; those with visible agglutination are judged as positive (+), and those with no agglutination and the reaction mixture is uniformly pink are judged as negative (-).

[0003] The isolation and culture of Brucella has always been the gold standard for laboratory diagnosis of brucellosis infection, but due to the long experimental time and high biosafety risks, it is not suitable for universal use. Currently, the most widely used serological detection methods in my country are the red bengal agglutination test (RBT) and SAT.

[0004] At present, brucellosis testing is mostly done manually. If the number of tests is large, the number of people required is large and the manual work intensity is high, which will also cause certain human operation errors. Errors may occur when adding serum and antigens, and the effect cannot be guaranteed to be consistent when mixing. In addition, there are certain safety risks in brucellosis testing experiments. Brucella can be transmitted through damaged skin mucosa, digestive tract and respiratory tract, etc., and there is a risk of infection in manual operation. Utility Model Content

[0005] The utility model aims to provide a fully automatic brucellosis antibody detection workstation, which can automatically perform detection, has a built-in shaking and oscillating function for mixing, ensures that all tested samples have the same mixing effect, and is equipped with a camera for automatic photography.

[0006] In order to achieve the above-mentioned purpose, the utility model proposes the following technical solution: a fully automatic brucellosis antibody detection workstation, a cabinet (1), and also includes:

[0007] A pipette (2), wherein the moving mechanism drives the pipette (2) to move in the cabinet (1);

[0008] A sample rack (3) and a sample tube (4), wherein the sample rack (3) is placed in the cabinet (1), and the sample tube (4) is placed on the sample rack (3);

[0009] A pipette tip box (5), wherein the pipette tip box (5) is used to provide pipette tip consumables;

[0010] A waste liquid tank (8), a disinfectant tank (13) and a reagent tank (14), wherein the waste liquid tank (8) is used to recover waste liquid and pipette tip consumables, the disinfectant tank (13) is used to provide disinfectant, and the reagent tank (14) is used to provide reagents;

[0011] An oscillating module (7) and an experimental board (6), wherein the experimental board (6) is placed on the oscillating module (7), and the oscillating module (7) drives the experimental board (6) to perform circular motion;

[0012] A photographing unit (9), wherein the photographing unit (9) is used to take a photograph of the experimental board (6).

[0013] Furthermore, in the present invention, the moving mechanism is a three-axis linear module.

[0014] Furthermore, in the utility model, the cabinet door (11) is movably connected to the cabinet body (1), and a hydraulic cylinder (10) is provided on the cabinet door (11).

[0015] Furthermore, in the utility model, a support seat (12) is provided at the bottom of the cabinet (1).

[0016] Furthermore, in the utility model, an ultraviolet lamp for disinfection is arranged inside the cabinet (1).

[0017] Beneficial effects: The technical solution of this application has the following technical effects:

[0018] The utility model can perform sample loading, precise pipetting, liquid transfer, well plate filling, reagent loading, mixing and shaking, reagent extraction, detection system construction, and sample photography in one, and can perform multi-area physical division according to the experimental sequence, time, and sample addition requirements of each step; avoid cross-contamination of samples, meet the requirements of high-throughput sample processing, can fully shake and mix, improve the accuracy of detection results, and greatly improve the efficiency of detection.

[0019] It should be appreciated that all combinations of the foregoing concepts, as well as additional concepts described in greater detail below, may be considered part of the inventive subject matter of the present disclosure, provided such concepts are not mutually inconsistent.

[0020] The foregoing and other aspects, embodiments and features of the present invention can be more fully understood from the following description in conjunction with the accompanying drawings. Other additional aspects of the present invention, such as the features and / or beneficial effects of the exemplary embodiments, will be apparent from the following description or learned from the practice of the specific embodiments according to the teachings of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The accompanying drawings are not intended to be drawn to scale. In the accompanying drawings, each identical or nearly identical component shown in various figures may be represented by the same reference numeral. For the sake of clarity, not every component is labeled in each figure. Now, embodiments of various aspects of the utility model will be described by way of example and with reference to the accompanying drawings, in which:

[0022] Figure 1 It is a schematic diagram of the structure of the utility model.

[0023] Figure 2 It is a schematic diagram of the structure of the utility model.

[0024] Figure 3 This is an experimental display diagram of the utility model.

[0025] In the figure, the meaning of each figure mark is as follows: 1. cabinet; 2. pipette; 3. sample rack; 4. sample tube; 5. tip box; 6. experimental plate; 7. shaking module; 8. waste liquid tank; 9. camera unit; 10. hydraulic cylinder; 11. cabinet door; 12. support base; 13. disinfectant tank; 14. reagent tank. DETAILED DESCRIPTION

[0026] In order to better understand the technical content of the utility model, specific embodiments are cited and described as follows in conjunction with the accompanying drawings. In this disclosure, various aspects of the utility model are described with reference to the accompanying drawings, and many illustrative embodiments are shown in the accompanying drawings. The embodiments of the present disclosure are not necessarily defined to include all aspects of the utility model. It should be understood that the various concepts and embodiments introduced above, as well as those described in more detail below, can be implemented in any of many ways, because the concepts and embodiments disclosed in the utility model are not limited to any implementation method. In addition, some aspects disclosed in the utility model can be used alone, or used in any appropriate combination with other aspects disclosed in the utility model.

[0027] like Figure 1-3 As shown, a fully automatic brucellosis antibody detection workstation includes a cabinet 1, a pipette 2, a sample rack 3, a sample tube 4, a tip box 5, an oscillation module 7, an experimental plate 6, a waste liquid tank 8, a camera unit 9, a disinfectant tank 13 and a reagent tank 14.

[0028] The moving mechanism drives the pipette 2 to move in the cabinet 1. The moving mechanism is a three-axis linear module, which can move in the X, Y, and Z axes.

[0029] The sample rack 3 is placed in the cabinet 1, located Figure 1 On the left side, the sample tubes 4 are placed on the sample rack 3 and are uniformly put in by the staff;

[0030] The tip box 5, the waste liquid tank 8, the disinfectant tank 13 and the reagent tank 14 are located in the middle position. The tip box 5 is used to provide tip consumables; the waste liquid tank 8 is used to recover waste liquid and tip consumables, the disinfectant tank 13 is used to provide disinfectant, and the reagent tank 14 is used to provide reagents;

[0031] The oscillation module 7 and the experimental board 6 are placed on the right side. The experimental board 6 is placed on the oscillation module 7 . The oscillation module 7 drives the experimental board 6 to perform circular motion. The photographing unit 9 is used to take photos of the experimental board 6 .

[0032] In this embodiment, the cabinet 1 is composed of a sheet metal structure and an acrylic structure, and the connection is fixed by a sheet metal bolt. The acrylic is black and translucent. There is an openable cabinet door 11 on the front. The upper part of the cabinet door 11 is fixed to the upper part by an invisible hinge. The upper part of the cabinet door 11 is fixed by a hydraulic rod 10, wherein the other end of the hydraulic rod 10 is fixed inside the door frame, and the two sides are symmetrically arranged. The hydraulic rod 10 can support the cabinet door 11 to open and close, and the cabinet door 11 is opened and supported by the hydraulic rod. The operating platform below the cabinet 1 is processed from a whole piece of aluminum plate, and above the operating platform are sample racks 3, sample tubes 4, suction head boxes 5, oscillation modules 7, experimental plates 6, waste liquid tanks 8, disinfectant tanks 13 and reagent tanks 14. The bottom of the cabinet 1 is provided with a support seat 12 to increase the stability of placement. Ultraviolet light disinfection is provided in the cabinet 1, and the ultraviolet light disinfection is turned on to ensure the safety of the entire experiment.

[0033] Experimental process 1

[0034] The fully automatic brucellosis antibody detection workstation in the present embodiment adopts the principle of brucellosis tiger red plate agglutination test, and the experiment is uniformly processed to reduce interference from other factors, so as to achieve uniform results and reduce errors. The computer equipped with the fully automatic brucellosis antibody detection workstation of the present invention is connected to the host computer through a network cable, and quick settings can be performed. Turn on the switch, start the dynamic workstation, and the three-axis linear module starts to move. Thereafter, the motor of the oscillation module drives the oscillation reaction table to rotate so that it touches the induction switch and performs a reset operation. When the three axes and the oscillation module trigger the induction switch, they stop, and all positions are reset to zero at this time.

[0035] After making relevant settings, the workstation will perform linear interpolation, and the X-axis and Y-axis of the three-axis linear module will move simultaneously to reach the tip box 5, and the Z-axis of the three-axis linear module will control the slide to move downward. In the following text, the X-axis, Y-axis, and Z-axis of the three-axis linear module are referred to as the X-axis, Y-axis, and Z-axis, thereby controlling the overall downward movement of the pipette 2. When it reaches a certain height, the double-channel tip of the pipette 2 can continue to move downward and touch the tip box 5. Under the action of pressure, the pipette 2 will be embedded in the tip box 5 and fix the tip.

[0036] At this time, the Z-axis controls the slide to move upward, and then controls the pipette 2 together with the pipette tip to move up, and performs linear interpolation. The X-axis and Y-axis move simultaneously. When the coordinates of the sample tube 4 are moved, the Z-axis controls the slide to move downward. When it descends to 5MM above the sample tube 4, the speed is reduced, and the liquid level detection is started. It slowly moves down to the inside of the sample tube 4. When the pipette 2 detects the liquid level, it stops and probes 3MM downward. The motor of the pipette 2 rotates in the opposite direction to absorb the liquid. After the absorbed liquid reaches the required standard, the motor of the pipette 2 stops rotating.

[0037] The Z axis controls the slide to move up, and at this time, the motor of the pipette 2 continues to rotate for several turns before stopping, sealing the internal liquid to prevent liquid leakage. Perform linear interpolation, with the X axis and Y axis moving simultaneously to reach the position of the experimental board 6. At this time, the Z axis controls the slide to move downward, and then controls the pipette 2 to move downward. When it drops to the suspension height of the experimental board 6, the motor of the pipette 2 rotates forward to discharge the liquid. After all the liquid is discharged, the motor of the pipette 2 stops rotating.

[0038] The Z-axis controls the slide to move up, and performs linear interpolation. The X-axis and Y-axis move simultaneously to reach the waste liquid tank 8. The Z-axis controls the slide to move downward, and then controls the pipette 2 to move downward as a whole. When it reaches a certain height, the motor of the pipette 2 moves forward, and the pipette tip on the pipette 2 is ejected to the waste liquid tank 8. Then repeat this operation until the sample is flattened, and start flattening the reagent.

[0039] Experimental process 2

[0040] The workstation will perform linear interpolation, with the X-axis and Y-axis moving simultaneously to reach the tip box 5. The Z-axis controls the slide to move downward, thereby controlling the overall downward movement of the pipette 2. When it reaches a certain height, the double-channel tip of the pipette 2 can continue to move downward and touch the tip box 5. Under the action of pressure, the pipette 2 will be embedded in the tip box 5 and fix the new tip.

[0041] At this time, the Z-axis controls the slide to move upward, and then controls the pipette 2 together with the pipette tip to move up, and performs linear interpolation. The X-axis and Y-axis move simultaneously. When it moves to the coordinate of the reagent tank 14, the Z-axis can control the slide to move downward. When it descends to 5MM above the reagent tank 14, the speed is reduced. When it is suspended above the reagent tank 14, the liquid level detection is started, and it slowly moves down to the inside of the reagent tank 14. When the pipette 2 detects the liquid level, it stops and probes downward to the lowest position of the reagent tank 14. The motor of the pipette 2 rotates in the opposite direction to absorb the reagent liquid, and then the motor rotates forward to discharge the liquid. The operation is repeated three times for mixing. After the reagent mixing is completed, the motor of the pipette 2 rotates in the opposite direction to absorb the liquid. After the absorbed liquid reaches the required standard, the motor of the pipette 2 stops rotating.

[0042] The Z axis controls the slide to move up, and at this time, the motor of the pipette 2 continues to rotate upward for several turns before stopping, sealing the internal liquid to prevent liquid leakage. Perform linear interpolation, with the X axis and Y axis moving simultaneously to reach the position of the experimental board 6. At this time, the Z axis controls the slide to move downward, and then controls the pipette 2 to move downward. When it drops to the suspension height of the experimental board 6, the motor of the pipette 2 rotates forward to discharge the liquid. The X axis moves forward with reference to the software deviation, and the pipette 2 discharges the liquid according to the software setting. After all the liquid is discharged, the motor of the pipette 2 stops rotating.

[0043] The Z-axis controls the slide to move up and performs linear interpolation. The X-axis and Y-axis move simultaneously to reach the waste liquid tank 8. The Z-axis controls the slide to move downward, thereby controlling the overall downward movement of the pipette 2. When it reaches a certain height, the motor of the pipette 2 moves forward to discharge the pipette 2 tip into the waste liquid tank 8.

[0044] Experimental process 3

[0045] At this time, the motor of the oscillation module 7 rotates forward, driving the eccentric shaft and the bearing to rotate, so that the experimental plate 6 performs a circular motion. After rotating for 90 seconds, the motor stops rotating and starts to stand for 4 minutes to wait for the sample and reagent in the experimental plate 6 to fully react. After the standing is completed, the X-axis and Y-axis move simultaneously to the specified position, and the Z-axis controls the slide to move down to the specified height. The camera unit (9) is a camera, and the camera automatically takes a photo and saves it. After the photo is taken, the ultraviolet lamp module is automatically turned on for overall disinfection, and then the power is turned off to complete the single experiment operation.

[0046] Experimental process 3

[0047] The X-axis and Y-axis move simultaneously to reach the disinfectant tank 13, and the Z-axis controls the slide to move downward, thereby controlling the overall downward movement of the pipette 2. When it reaches a certain height, the motor of the pipette 2 rotates in the opposite direction to absorb the disinfectant. When the absorbed liquid reaches the required standard, the motor of the pipette 2 stops rotating. The Z-axis controls the slide to move upward, and performs linear interpolation. The X-axis and Y-axis move simultaneously to reach the positive sample position. The motor of the pipette 2 rotates forward to discharge the liquid. The X-axis and Y-axis move forward with reference to the software deviation. The pipette 2 discharges the liquid according to the software setting. After all the liquid is discharged, the motor of the pipette 2 stops rotating and returns to the disinfectant tank 13 to repeat the action until the disinfection of the positive sample at the sample position is completed.

[0048] Then, the positive samples of the experimental plate 6 are disinfected. The pipette 2 returns to the disinfectant tank 13 and moves down to a certain height. The motor of the pipette 2 moves forward to discharge the excess disinfectant, and then the motor of the pipette 2 rotates in the opposite direction to absorb the disinfectant. After the absorbed liquid reaches the required standard, the motor of the pipette 2 stops rotating. The Z-axis controls the slide to move up, and the linear interpolation method is performed. The X-axis and the Y-axis move simultaneously to reach the positive sample position of the reaction plate. The motor of the pipette 2 rotates forward to discharge the liquid. The X-axis and the Y-axis move forward with reference to the software deviation. The pipette 2 discharges the liquid according to the software setting. After all the liquid is discharged, the motor of the pipette 2 stops rotating and returns to the disinfectant tank 13 to repeat the action until the positive sample of the reaction plate is disinfected. The Z-axis controls the slide to move up. By linear interpolation, the X-axis and Y-axis move simultaneously to reach the waste liquid tank 8, and the Z-axis controls the slide to move downward, thereby controlling the overall downward movement of the pipette 2. When it reaches a certain height, the motor of the pipette 2 moves forward to spray the pipette tip into the waste liquid tank 8. After the disinfection is completed, the ultraviolet lamp module is automatically turned on for overall disinfection, and then the power is turned off to complete a single experimental operation.

[0049] The standard parts used in this application document can all be purchased from the market and can be customized according to the description in the specification and drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the prior art. The machinery, parts and equipment all adopt conventional models in the prior art. The control method is automatic control through a controller. The control circuit of the controller can be implemented through simple programming by technicians in this field, which is common knowledge in the field. This application is mainly used to protect mechanical devices, so this application no longer explains the control method and circuit connection in detail.

[0050] Although the present invention has been disclosed as above with preferred embodiments, it is not intended to limit the present invention. A person with ordinary knowledge in the technical field to which the present invention belongs may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be determined by the definition of the claims.

Claims

1. A fully automatic brucellosis antibody detection workstation, cabinet (1), characterized in that: Also includes: A pipette (2), wherein the moving mechanism drives the pipette (2) to move in the cabinet (1); A sample rack (3) and a sample tube (4), wherein the sample rack (3) is placed in the cabinet (1), and the sample tube (4) is placed on the sample rack (3); A pipette tip box (5), wherein the pipette tip box (5) is used to provide pipette tip consumables; A waste liquid tank (8), a disinfectant tank (13) and a reagent tank (14), wherein the waste liquid tank (8) is used to recover waste liquid and pipette tip consumables, the disinfectant tank (13) is used to provide disinfectant, and the reagent tank (14) is used to provide reagents; An oscillating module (7) and an experimental board (6), wherein the experimental board (6) is placed on the oscillating module (7), and the oscillating module (7) drives the experimental board (6) to perform circular motion; A photographing unit (9), wherein the photographing unit (9) is used to take a photograph of the experimental board (6).

2. A fully automatic brucellosis antibody detection workstation according to claim 1, characterized in that: The moving mechanism is a three-axis linear module.

3. A fully automatic brucellosis antibody detection workstation according to claim 1, characterized in that: It also comprises a cabinet door (11), the cabinet door (11) being movably connected to the cabinet body (1), and a hydraulic cylinder (10) being arranged on the cabinet door (11).

4. A fully automatic brucellosis antibody detection workstation according to claim 1, characterized in that: A support seat (12) is provided at the bottom of the cabinet (1).

5. A fully automatic brucellosis antibody detection workstation according to claim 1, characterized in that: An ultraviolet lamp for disinfection is arranged inside the cabinet (1).

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