Pipetting mechanism for slide preparation

Abstract

The utility model provides a pipetting mechanism for slide preparation, which comprises a lower extension rod connecting plate capable of vertically moving, a pipette connector is arranged on the lower extension rod connecting plate, an air duct is arranged in the pipette connector, an air guide hole is formed in the upper end of the air duct, a connecting nozzle is arranged at the lower end of the air duct, and the connecting nozzle is provided with a plurality of cylindrical surface sleeving parts arranged in a step shape. A flange edge is arranged on the outer edge of the upper end of the transfer pipette, a pipette discharging frame is arranged on one side of the transfer pipette connector, a shifting piece is arranged at the lower end of the pipette discharging frame, the end of the shifting piece is attached to the outer wall of the connecting nozzle, and the problem of automatic pipetting of the slide preparation equipment is solved.

Description

Technical Field

[0001] This utility model relates to the field of liquid-based slide preparation, and in particular to a liquid transfer mechanism for glass slide preparation. Background Technology

[0002] In liquid-based slide preparation technology, cells or tissues are collected using a brush or other collection tools, and the samples are stored in a sampling bottle containing a preservation solution. The bottle is then sealed and transported to the slide preparation station, where necessary reagents are added to the cell samples. The samples are then transferred to the slide preparation chamber of a centrifuge for centrifugation and are made into glass slides.

[0003] In automated slide preparation equipment, due to the large workload and high precision requirements, traditional manual operation can no longer meet the needs of quantitative reagent addition and sample transfer. Furthermore, manual operation may lead to sample contamination, which in turn could potentially infect humans. Additionally, automated slide preparation requires various reagent types and inconsistent reagent bottle specifications, necessitating different dosages and different sizes of pipettes.

[0004] Therefore, there is an urgent need for an automated pipetting device that can safely and reliably meet the needs of the pipetting process. Utility Model Content

[0005] This invention provides a liquid transfer mechanism for glass slide preparation, which solves the problem of automated liquid transfer in glass slide preparation equipment.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is: a pipetting mechanism for glass slide preparation, including a vertically movable lower extension rod connecting plate, a pipetting tube connector provided on the lower extension rod connecting plate, a venting channel provided inside the pipetting tube connector, a venting hole provided at the upper end of the venting channel, a connecting nozzle provided at the lower end of the venting channel, and multiple cylindrical sleeve parts arranged in a stepped manner provided at the connecting nozzle, the cylindrical sleeve parts being used to engage with the pipetting tube, a flange provided at the outer edge of the upper end of the pipetting tube, a tube removal bracket provided on one side of the pipetting tube connector, a lever provided at the lower end of the tube removal bracket, and the end of the lever abutting against the outer wall of the connecting nozzle.

[0007] In a preferred embodiment, a third linear motion mechanism is also included. The third linear motion mechanism includes a base frame, on which a lifting frame plate that can move up and down is provided. A lower extension rod is provided on the lifting frame plate, and the lower end of the lower extension rod is connected to a lower extension rod connecting plate. A lower extension rod guide seat is provided on the base frame, and the lower extension rod guide seat is slidably sleeved with the lower extension rod. A lifting plate guide rod is provided at the upper end of the pipe unloading frame, and a guide rod guide seat is also provided on the base frame. The lifting plate guide rod is slidably sleeved with the guide rod guide seat. A spring balancer is provided at the upper end of the base frame, and the spring balancer suspends the upper end of the lifting plate guide rod.

[0008] In a preferred embodiment, the lower end of the pipette rack has a hollow section with a hinge shaft at the hollow section. One end of the lever is hinged to the hinge shaft, and the lever has a first countersunk hole. A baffle is provided on the side of the hinge shaft away from the pipette connector, and a second countersunk hole is provided inside the baffle. A spring is also provided, with one end of the spring inserted into the first countersunk hole and abutting against the bottom of the first countersunk hole, and the other end of the spring inserted into the second countersunk hole and abutting against the bottom of the second countersunk hole.

[0009] In the preferred embodiment, the lower extension rod connecting plate is provided with a positioning hole, the upper end of the pipette connector is provided with a positioning protrusion, the positioning protrusion is sleeved with the positioning hole, the outer wall of the positioning protrusion is provided with an annular groove, and the side wall of the positioning hole is provided with a threaded locking screw, which is inserted into the annular groove and presses against the positioning protrusion.

[0010] In the preferred embodiment, the positioning protrusion has a notch at the air guide hole, and the air guide hole has an air connector.

[0011] In a preferred embodiment, a third linear motion mechanism is also included. The third linear motion mechanism includes a base frame, on which a second lead screw and a third guide rail slider device are provided. A second lead screw nut is provided on one side of the lifting frame plate. The second lead screw nut is sleeved with the second lead screw. One end of the second lead screw nut is slidably connected to the base frame through the third guide rail slider device. A third drive motor is also provided on the base frame, and the shaft end of the third drive motor is connected to the second lead screw.

[0012] In a preferred embodiment, a first linear motion mechanism and a second linear motion mechanism are further provided. The first linear motion mechanism includes a transverse frame and a base plate. A first guide rail slider device is provided on the base plate. The transverse frame and the base plate are slidably connected through the first guide rail slider device. A first lead screw is provided on the base plate. A first drive motor is provided at the shaft end of the first lead screw. A first lead screw nut is also provided on the side of the transverse frame near the base plate. The first lead screw nut is sleeved with the first lead screw. The second linear motion mechanism includes a cantilever frame. A second guide rail slider device is provided on the cantilever frame. A base frame is slidably connected to the cantilever frame through the second guide rail slider device. A second drive motor is provided on the cantilever frame. A synchronous belt mechanism is provided on the cantilever frame. A synchronous belt clamping block is provided on the base frame. The second drive motor drives the base frame to move through the synchronous belt mechanism.

[0013] The beneficial effects of this utility model are as follows: a detachable pipette is connected by a pipette connector, and the pipette connector is provided with cylindrical sleeves of various diameters to accommodate various pipette specifications; the lever can swing and always stays close to the outer wall of the connector; after pipetting is completed, the pipette is automatically removed by the lever and replaced with a new clean pipette, avoiding cross-contamination of the sample. Attached Figure Description

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0015] Figure 1This is a schematic diagram of a pipette and its Cartesian three-dimensional movement mechanism.

[0016] Figure 2 This is a schematic diagram of the up-and-down movement mechanism of a pipette.

[0017] Figure 3 This is a partial view of the pipette connector.

[0018] Figure 4 This is a schematic diagram of the pipette connector and the pipette unloading mechanism.

[0019] Figure 5 This is a cross-sectional view of the connector.

[0020] Figure 6 This is a diagram showing the non-working position of the paddle shifter.

[0021] Figure 7 This is a schematic diagram of the lowest working position of the paddle shifter.

[0022] In the figure: First linear movement mechanism 1; transverse frame 101; base plate 102; first guide rail slider device 103; first drive motor 104; first lead screw 105; first lead screw nut 106; second linear movement mechanism 2; base frame 201; cantilever frame 202; second guide rail slider device 203; second drive motor 204; synchronous belt mechanism 205; synchronous belt clamping block 206; third linear movement mechanism 3; lifting frame plate 301; third drive motor 302; second lead screw 303; second lead screw nut 304; third guide rail slider device 305; lower extension rod 4 ; Lower extension rod connecting plate 401; Lower extension rod guide seat 402; Positioning hole 403; Locking screw 404; Pipe connector 5; Connecting nozzle 501; Cylindrical sleeve part 502; Air guide hole 503; Positioning protrusion 504; Annular groove part 505; Notch part 506; Air connector 507; Unloading rack 6; Paddle 601; Lifting plate guide rod 602; Guide rod guide seat 603; Slot part 604; Hollowed-out part 605; Hinge shaft 606; First countersunk hole 607; Spring 608; Baffle 609; Second countersunk hole 610; Pipe 7; Flange edge 701; Spring balancer 8. Detailed Implementation

[0023] like Figure 1-7 In the present invention, a pipetting mechanism for glass slide preparation includes a vertically movable lower extension rod connecting plate 401, a pipetting tube connector 5 is provided on the lower extension rod connecting plate 401, a venting channel is provided inside the pipetting tube connector 5, a venting hole 503 is provided at the upper end of the venting channel, a connecting nozzle 501 is provided at the lower end of the venting channel, the connecting nozzle 501 is provided with a plurality of cylindrical sleeve portions 502 arranged in a stepped manner, the cylindrical sleeve portions 502 are used to sleeve with a pipetting tube 7, a flange edge 701 is provided on the upper outer edge of the pipetting tube 7, a tube unloading bracket 6 is provided on one side of the pipetting tube connector 5, a lever 601 is provided at the lower end of the tube unloading bracket 6, and the end of the lever 601 abuts against the outer wall of the connecting nozzle 501.

[0024] 5 has a cavity structure and air guide holes at both the upper and lower ends. The air guide hole at the lower end is connected to the connecting nozzle 501.

[0025] The diameter of the lower cylindrical sleeve portion 502 is slightly smaller than that of the upper cylindrical sleeve portion 502, so the connecting nozzle 501 can be fitted with various pipettes 7 of different diameters.

[0026] The pipette 7 adopts a customized structure with an outwardly protruding flange 701 on the outer edge of the upper port. When the lower extension rod connecting plate 401 moves the pipette connector 5 upward, the paddle 601 has a slot 604. The slot 604 abuts against the flange 701 and scrapes the pipette 7 off.

[0027] The vent 503 can be switched between negative and positive pressure. When the pipette 7 is inserted into the reagent bottle, the vent 503 is connected to negative pressure to draw in liquid. Subsequently, when the pipette 7 is moved above the sample bottle, the vent 503 is connected to positive pressure, and the liquid in the pipette 7 is discharged into the sample bottle.

[0028] In a preferred embodiment, a third linear motion mechanism 3 is also included. The third linear motion mechanism 3 includes a base frame 201, on which a vertically movable lifting frame plate 301 is provided. A lower extension rod 4 is provided on the lifting frame plate 301, and the lower end of the lower extension rod 4 is connected to a lower extension rod connecting plate 401. A lower extension rod guide seat 402 is provided on the base frame 201, and the lower extension rod guide seat 402 is slidably sleeved with the lower extension rod 4. A lifting plate guide rod 602 is provided at the upper end of the pipe unloading frame 6. A guide rod guide seat 603 is also provided on the base frame 201, and the lifting plate guide rod 602 is slidably sleeved with the guide rod guide seat 603. A spring balancer 8 is provided at the upper end of the base frame 201, and the spring balancer 8 suspends the upper end of the lifting plate guide rod 602.

[0029] When the pipette connector 5 moves downward, its lower end abuts against the lever 601, causing the pipette rack 6 to descend. The connector 501 is inserted into the pipette 7 on the lower pipette rack. Since the pipette 7 is made of plastic, it has a certain degree of elasticity. The beveled structure at the lower end of the cylindrical sleeve 502 is inserted into the pipette 7 first, and the pipette opening expands slightly and fits onto the cylindrical sleeve 502, which can form a seal and has a certain clamping force.

[0030] Subsequently, the lifting platform 301 moves upward, causing the pipette 7 to detach from the pipe rack. The spring balancer 8 adjusts the tension to be slightly greater than the total weight of the unloading rack 6 and related structures. Under the action of the spring balancer 8, the unloading rack 6 moves upward and resets along with the pipette connector 5. When the upper end of the unloading rack 6 is stopped by the guide rod guide seat 603, it no longer moves upward. At this time, the pipette connector 5 continues to move upward, and the lever 601 can scrape off the pipette 7.

[0031] In a preferred embodiment, the lower end of the tube unloading rack 6 is provided with a hollow portion 605, and a hinge shaft 606 is provided at the hollow portion 605. One end of the lever 601 is hinged to the hinge shaft 606. The lever 601 is provided with a first countersunk hole 607. A baffle 609 is provided on the side of the hinge shaft 606 away from the pipette connector 5. A second countersunk hole 610 is provided in the baffle 609. A spring 608 is also provided. One end of the spring 608 is inserted into the first countersunk hole 607 and abuts against the bottom end of the first countersunk hole 607. The other end of the spring 608 is inserted into the second countersunk hole 610 and abuts against the bottom end of the second countersunk hole 610.

[0032] Under the elastic force of spring 608, the end of the paddle 601 is always tightly pressed against the outer wall of the connector 501. No matter what diameter pipette 7 is fitted onto the connector 501, the slot 604 can abut against the cylindrical sleeve 502 of the next level. When the connector 501 moves upward relative to the paddle 601, the pipette 7 is scraped off.

[0033] In the preferred embodiment, the lower extension rod connecting plate 401 is provided with a positioning hole 403, the upper end of the pipette connector 5 is provided with a positioning protrusion 504, the positioning protrusion 504 is sleeved with the positioning hole 403, the outer wall of the positioning protrusion 504 is provided with an annular groove 505, the side wall of the positioning hole 403 is provided with a threaded locking screw 404, the locking screw 404 is inserted into the annular groove 505 and presses against the positioning protrusion 504.

[0034] In the preferred embodiment, the positioning protrusion 504 has a notch 506 at the air guide hole 503, and the air guide hole 503 has an air connector 507.

[0035] The air connector 507 is used to connect to an external negative pressure and positive pressure switching air pipe.

[0036] In a preferred embodiment, a third linear motion mechanism 3 is also included. The third linear motion mechanism 3 includes a base frame 201, on which a second lead screw 303 and a third guide rail slider device 305 are provided. A second lead screw nut 304 is provided on one side of the lifting frame plate 301. The second lead screw nut 304 is sleeved with the second lead screw 303. One end of the second lead screw nut 304 is slidably connected to the base frame 201 through the third guide rail slider device 305. A third drive motor 302 is also provided on the base frame 201. The shaft end of the third drive motor 302 is connected to the second lead screw 303.

[0037] In a preferred embodiment, a first linear moving mechanism 1 and a second linear moving mechanism 2 are further provided. The first linear moving mechanism 1 includes a transverse frame 101 and a base plate 102. A first guide rail slider device 103 is provided on the base plate 102. The transverse frame 101 and the base plate 102 are slidably connected by the first guide rail slider device 103. A first lead screw 105 is provided on the base plate 102. A first drive motor 104 is provided at the shaft end of the first lead screw 105. A first lead screw nut 106 is also provided on the side of the transverse frame 101 near the base plate 102. The lead screw nut 106 is sleeved with the first lead screw 105. The second linear movement mechanism 2 includes a cantilever 202. The cantilever 202 is provided with a second guide rail slider 203. The base frame 201 is slidably connected to the cantilever 202 through the second guide rail slider 203. The cantilever 202 is provided with a second drive motor 204 and a synchronous belt mechanism 205. The base frame 201 is provided with a synchronous belt clamping block 206. The second drive motor 204 drives the base frame 201 to move through the synchronous belt mechanism 205.

[0038] The first linear movement mechanism 1 is installed on the frame of the slide preparation machine. The first linear movement mechanism 1, the second linear movement mechanism 2 and the third linear movement mechanism 3 constitute a Cartesian movement mechanism, which drives the pipette connector 5 to move freely in three orthogonal directions, ensuring that liquid can be aspirated, pipetted and added at any position in the space of the slide preparation equipment.

[0039] The above embodiments are merely preferred technical solutions of this utility model and should not be considered as limitations on this utility model. The protection scope of this utility model should be the technical solution described in the claims, including equivalent substitutions of the technical features described in the claims. That is, equivalent substitutions and improvements within this scope are also within the protection scope of this utility model.

Claims

1. A pipetting mechanism for slide preparation, characterized by: The lower extension rod connecting plate (401) is vertically movable, and a pipette connector (5) is arranged on the lower extension rod connecting plate (401); the pipette connector (5) is internally provided with a ventilation channel, the upper end of the ventilation channel is provided with a gas guide hole (503), the lower end of the ventilation channel is provided with a connecting nozzle (501), the connecting nozzle (501) is provided with a plurality of stepped cylindrical sleeve joints (502), the cylindrical sleeve joints (502) are used for sleeving with a pipette (7), the outer edge of the upper end of the pipette (7) is provided with a flange (701), one side of the pipette connector (5) is provided with a pipe removal rack (6), the lower end of the pipe removal rack (6) is provided with a push piece (601), and the end of the push piece (601) abuts against the outer wall of the connecting nozzle (501).

2. The pipette mechanism for slide preparation of claim 1, wherein: The third linear movement mechanism (3) comprises a base frame (201), the base frame (201) is provided with a vertically movable lifting frame plate (301), the lifting frame plate (301) is provided with a lower extension rod (4), the lower end of the lower extension rod (4) is connected with the lower extension rod connecting plate (401), the base frame (201) is provided with a lower extension rod guide seat (402), the lower extension rod guide seat (402) is slidably sleeved with the lower extension rod (4), the upper end of the pipe removal rack (6) is provided with a lifting plate guide rod (602), the base frame (201) is further provided with a guide rod guide seat (603), the lifting plate guide rod (602) is slidably sleeved with the guide rod guide seat (603), and the upper end of the base frame (201) is provided with a spring balancer (8).

3. The pipette mechanism for slide preparation of claim 1, wherein: The lower end of the pipe removal rack (6) is provided with a hollow part (605), the hollow part (605) is provided with a hinge shaft (606), one end of the push piece (601) is hinged with the hinge shaft (606), the push piece (601) is provided with a first counterbore (607), the side, away from the pipette connector (5), of the hinge shaft (606) is provided with a baffle (609), the baffle (609) is internally provided with a second counterbore (610), and a spring (608) is further arranged, one end of the spring (608) is inserted into the first counterbore (607) and abuts against the bottom end of the first counterbore (607), and the other end of the spring (608) is inserted into the second counterbore (610) and abuts against the bottom end of the second counterbore (610).

4. The pipette device for slide preparation of claim 1, wherein: The lower extension rod connecting plate (401) is provided with a positioning hole (403), the upper end of the pipette connector (5) is provided with a positioning convex column (504), the positioning convex column (504) is sleeved with the positioning hole (403), the outer wall of the positioning convex column (504) is provided with a ring groove part (505), the side wall of the positioning hole (403) is provided with a locking jack screw (404) in threaded connection, and the locking jack screw (404) is inserted into the ring groove part (505) and tightly abuts against the positioning convex column (504).

5. The pipette mechanism for slide preparation of claim 4, wherein: The positioning convex column (504) is provided with a notch part (506) at the gas guide hole (503), and a gas connector (507) is arranged at the gas guide hole (503).

6. The pipette device for slide preparation of claim 2, wherein: The third linear moving mechanism (3) comprises a base frame (201), the second screw rod (303) and the third guide rail sliding block device (305) are arranged on the base frame (201), the second nut seat (304) is arranged on one side of the lifting frame plate (301), the second nut seat (304) is sleeved with the second screw rod (303), one end of the second nut seat (304) is slidably connected with the base frame (201) through the third guide rail sliding block device (305), the third driving motor (302) is further arranged on the base frame (201), and the shaft end of the third driving motor (302) is connected with the second screw rod (303).

7. The pipette mechanism for slide preparation of claim 6, wherein: The first linear moving mechanism (1) and the second linear moving mechanism (2) are further arranged, the first linear moving mechanism (1) comprises a transverse moving frame (101) and a bottom plate (102), the first guide rail sliding block device (103) is arranged on the bottom plate (102), the transverse moving frame (101) and the bottom plate (102) are slidably connected through the first guide rail sliding block device (103), the first screw rod (105) is arranged on the bottom plate (102), the shaft end of the first screw rod (105) is provided with the first driving motor (104), the first nut seat (106) is further arranged on one side of the transverse moving frame (101) close to the bottom plate (102), the first nut seat (106) is sleeved with the first screw rod (105), the second linear moving mechanism (2) comprises a cantilever frame (202), the second guide rail sliding block device (203) is arranged on the cantilever frame (202), the base frame (201) is slidably connected with the cantilever frame (202) through the second guide rail sliding block device (203), the second driving motor (204) is arranged on the cantilever frame (202), the synchronous belt mechanism (205) is arranged on the cantilever frame (202), the synchronous belt clamping block (206) is arranged on the base frame (201), and the base frame (201) is driven to move by the second driving motor (204) through the synchronous belt mechanism (205).

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