Bacterial colony collecting device

Abstract

The utility model relates to the technical field of bacterial culture and collection, in particular to a bacterial colony collecting device which comprises a pipe body, and an air pressure control branch pipe is arranged on a pipe body of the pipe body. The air plug assembly is detachably installed on the air pressure control branch pipe. The piston push rod assembly is installed in the first end of the pipe body. The obtaining head is installed at the opening of the second end of the main tube body and is of a tubular structure, an inner cavity of the obtaining head communicates with an inner cavity of the tube body, and the tail end, away from the tube body, of the obtaining head is an annular blade part. The bacterial colony collecting device can obtain a single bacterial colony, keeps the morphological structure characteristics of the bacterial colony, can conveniently obtain a complete single bacterial colony, and is particularly suitable for bacterial colonies cultured by a water base and a soft base.

Description

Technical Field

[0001] This utility model relates to the field of bacterial culture and collection technology, and in particular to a colony collection device. Background Art

[0002] Existing colony collection devices are mostly scraper-type collectors, such as those used for collecting fully cultured colonies in hard glass containers. However, using a scraper to collect colonies can damage their morphological structure. Furthermore, on some soft culture media, such as agar, using a scraper to obtain colonies requires precisely cutting the agar blocks, making it difficult to preserve the colony's morphological characteristics. Utility Model Content

[0003] To address the aforementioned problems, this invention proposes a colony collection device that can acquire individual colonies while preserving their morphological and structural characteristics.

[0004] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0005] A colony collection device, comprising

[0006] The pipe body has a pneumatic control branch pipe on its body;

[0007] An air plug assembly, which is detachably mounted on a pressure control branch pipe;

[0008] Piston push rod assembly, which is installed inside the first end of the tube body;

[0009] The acquisition head is installed at the second end opening of the tube body. The acquisition head is a tubular structure with its internal cavity communicating with the internal cavity of the tube body, and the end of the acquisition head away from the tube body is a ring-shaped blade.

[0010] Preferably, the tube body and the acquisition head are detachably connected.

[0011] Preferably, the tube body and the receiving head are connected by threads or plugs.

[0012] Preferably, the connection between the tube body and the receiving head is sealed.

[0013] Preferably, the main body of the acquisition head is a base tube, with a reduced diameter section in the middle and a cutting section at the front end.

[0014] Preferably, the wall thickness of the cutting section is less than the wall thickness of the base pipe.

[0015] Preferably, the upper end of the tube body has a three-way structure, with the first passage being a piston control branch pipe that cooperates with the piston push rod assembly, and the second passage being a pneumatic control branch pipe. The tube body, the piston control branch pipe, and the pneumatic control branch pipe form a "Y" shaped structure.

[0016] The beneficial effects of using this utility model are:

[0017] This colony collection device allows for the control of internal air pressure via a pressure-controlled branch tube. It is suitable for collecting single colonies from general liquid surfaces, and can also be used to directly cut agar-like soft culture media without operating the pressure-controlled branch tube. This colony collection device can conveniently and completely collect single colonies, and is particularly suitable for colonies cultured on water-based and soft substrates. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the colony collection device of this utility model.

[0019] Figure 2 for Figure 1 A schematic diagram of obtaining the head.

[0020] Figure 3 This is a schematic diagram of the colony collection device in another embodiment.

[0021] The reference numerals in the figures include:

[0022] 10-Pipe body, 11-Pneumatic control branch pipe, 12-First sealing connection structure, 13-Piston control branch pipe, 20-Piston push rod assembly, 30-Acquisition head, 31-Base pipe, 32-Reduced diameter section, 33-Cutting section, 331-Cutting edge, 34-Storage cavity, 35-Second sealing connection structure, 40-Pneumatic plug assembly, 41-Soft connector, 42-Pneumatic plug head. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of this technical solution clearer, the following detailed description, in conjunction with specific embodiments, further illustrates this technical solution. It should be understood that these descriptions are merely exemplary and not intended to limit the scope of this technical solution.

[0024] To address the difficulty of collecting individual colonies from the surface of liquid or soft culture media using existing scrapers, such as... Figure 1 As shown, this embodiment proposes a colony collection device. This colony collection device adopts a principle similar to a syringe, which can obtain individual colonies relatively completely.

[0025] Specifically, the main body of this colony collection device is a tube 10, which is a straight glass tube with open ends at both the top and bottom. A piston push rod assembly 20 is detachably installed at the top of the tube 10. Similar to the piston assembly of a syringe in the prior art, the piston push rod assembly 20 can be manually pushed and pulled to change the internal air pressure of the tube 10. A pressure control branch tube 11 is provided on the side of the tube 10. The first end of the pressure control branch tube 11 is connected to the tube 10 and internally interconnected. An air plug assembly 40 is installed at the second end of the pressure control branch tube 11. The air plug assembly 40 includes an air plug head 42 and a flexible connector 41 connecting the air plug head 42 to the pressure control branch tube 11. The flexible connector 41 is used to prevent the air plug head 42 from being lost. The air plug head 42 can be inserted into the second end of the pressure control branch tube 11 to achieve a temporary seal.

[0026] like Figure 2 As shown, the lower end of the tube body 10 is a harvesting head 30, which can collect colonies on the surface of liquid or soft culture medium. Specifically, the harvesting head 30 is a rigid tube body 10, which can be a metal tube or a glass tube. In this embodiment, a glass tube is preferred. The main body of the harvesting head 30 is a base tube 31. The upper end of the base tube 31 can be inserted into the lower end of the tube body 10 for a detachable connection. The middle section of the base tube 31 is a tapered section 32, which has a tapered structure. The outer and inner diameters of the lower end are smaller than the outer and inner diameters of the main body of the base tube 31. The lower part of the tapered section 32 forms a cutting section 33, which is easier to insert into the soft culture medium. The transition between the lower end face and the outer peripheral face of the cutting section 33 is beveled and forms a blade 331 to facilitate cutting the soft culture medium. Inside the harvesting head 30, there is a storage cavity 34, the lower end of which is a tube body 10 with a smaller diameter and the upper part is a tube body 10 with a larger diameter.

[0027] In this embodiment, both the tube body 10 and the acquisition head 30 are preferably transparent glass tubes, which facilitates observation of the acquisition of colonies and the morphology of the colonies inside them.

[0028] The connection between the tube body 10 and the acquisition head 30 is a primary sealing structure with low sealing requirements. For example, the inner wall at the lower end of the tube body 10 has a first sealing connection structure 12, and the outer ring of the base tube 31 has a second sealing connection structure 35 near the upper end. In one feasible embodiment, both the first sealing connection structure 12 and the second sealing connection structure 35 can be frosted surfaces, forming a seal through the mutual contact of the frosted surfaces. In other feasible embodiments, both the first sealing connection structure 12 and the second sealing connection structure 35 can be threaded structures or silicone sleeves, achieving a seal through the cooperation of the threaded structure or the silicone sleeve.

[0029] When collecting colonies from the liquid surface, remove the air plug head 42 and lower the acquisition head 30 to the corresponding colony position until the lower end of the acquisition head 30 enters the liquid. The target colony is completely inside the cavity of the cutting section 33. During this process, the user does not need to pay attention to or control the piston push rod assembly 20; they only need to focus on the cutting section 33. Then, install the air plug head 42 at the end of the pneumatic control branch pipe 11, pull the piston push rod assembly 20, and the acquisition head 30 will draw in some liquid, lifting the colony to the connection between the narrowed section 32 and the cutting section 33. The colony collection device can be moved slowly without leakage. To discharge the colony, simply push the piston push rod assembly 20 down.

[0030] Obtaining colonies from the surface of soft culture media, such as agar, is relatively simple. Due to the stability of soft culture media, the acquisition head 30 is vertically inserted into the media via the blade 331, and a small section can be pulled upwards directly using the piston pusher assembly 20. If the soft culture medium is thin, it can be directly cut through the blade 331. If the soft culture medium is thick, after the target colony enters the cutting section 33, the piston pusher assembly 20 is pulled and the tube 10 is rotated to cut or break the soft culture medium through the blade 331. The target colony is relatively stable within the cutting section 33 and is restrained by it, preventing it from tipping over. Because the soft culture medium has a certain level of moisture, it is maintained within the medium by surface tension and will not fall off naturally. The soft culture medium can only be discharged from the acquisition head 30 by controlling the piston pusher assembly 20.

[0031] like Figure 3 As shown, this embodiment changes the positions of the pneumatic control branch pipe 11 and the piston pusher assembly 20. Specifically, the upper end of the pipe body 10 has a three-way structure, with the first passage being the piston control branch pipe 13 that cooperates with the piston pusher assembly 20, and the second passage being the pneumatic control branch pipe 11. The pipe body 10, the piston control branch pipe 13, and the pneumatic control branch pipe 11 form a "Y" shape. In this embodiment, both the piston pusher assembly 20 and the air plug assembly 40 are located at the top, making them easier to operate.

[0032] The above content is only a preferred embodiment of this utility model. For those skilled in the art, many changes can be made in the specific implementation and application scope based on the ideas of this technical content. As long as these changes do not depart from the concept of this utility model, they all fall within the protection scope of this patent.

Claims

1. A bacterial colony collection device, characterized in that: include A pipe body, wherein an air pressure control branch pipe is provided on the pipe body of the pipe body; An air plug assembly, the air plug assembly being detachably mounted on the air pressure control branch pipe; A piston push rod assembly is mounted inside the first end of the tubular body; The acquisition head is installed at the second end opening of the tube body. The acquisition head is a tubular structure, the internal cavity of which is connected with the internal cavity of the tube body, and the end of the acquisition head away from the tube body is an annular blade.

2. The bacterial colony collection device according to claim 1, characterized in that: The tube body and the acquisition head are detachably connected.

3. The bacterial colony collection device according to claim 2, characterized in that: The tube body and the acquisition head are connected by threads or plugs.

4. The bacterial colony collection device according to claim 3, characterized in that: The connection between the tube body and the acquisition head is sealed.

5. The bacterial colony collection device according to claim 1, characterized in that: The main body of the acquisition head is a base pipe, the middle part of the base pipe is provided with a reduced diameter section, and the front end forms a cutting section.

6. The bacterial colony collection device according to claim 5, characterized in that: The wall thickness of the cutting section is smaller than the wall thickness of the base pipe.

7. The bacterial colony collection device according to any one of claims 1 to 6, characterized in that: The upper end of the tube body is a three-way structure, the first passage of which is a piston control branch pipe that cooperates with the piston push rod assembly, and the second passage is an air pressure control branch pipe. The tube body, the piston control branch pipe, and the air pressure control branch pipe form a "Y" shape structure.

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