Rapid chromatographic equipment for industrial sephadex column

Abstract

The utility model discloses industrial dextran gel column rapid chromatography equipment which comprises an equipment shell, a chromatographic column and a chromatographic column, the flow dividing component comprises a first supporting ring, a second supporting ring, a third supporting ring, a flow dividing plate, a vertical rod, a supporting block, a rotating rod and a rotating shaft, the first supporting ring, the second supporting ring and the third supporting ring are installed on the inner surface of the equipment shell, the vertical rod is fixed to the top of the second supporting ring and the top of the third supporting ring, and the rotating rod is located on the upper surface of the vertical rod. According to the sephadex column chromatography disclosed by the utility model, a mixture is separated according to molecular size difference by utilizing a three-dimensional network structure of gel particles, small molecular substances can enter internal pores of the gel particles and stay in the column for a long time, macromolecular substances are excluded outside the particles and rapidly flow out along with a mobile phase, and meanwhile, a high-viscosity sample is poor in flowability, so that the sephadex column chromatography can be used for separating the mixture. The particles are slowly diffused in the column and are easily attached to the surfaces of gel particles to hinder mass transfer of other molecules.

Description

Technical Field

[0001] The utility model relates to the technical field of biochemical separation equipment, in particular to industrial dextran gel column rapid chromatography equipment. Background Art

[0002] In the field of biological separation and purification, efficient and precise technical means are crucial to obtaining high-purity biomacromolecules. As a classic and commonly used technology, dextran gel column chromatography occupies a pivotal position in biological science research and industrial production due to its unique separation principle and significant advantages.

[0003] Dextran gel column chromatography utilizes the three-dimensional network structure of gel particles to separate mixtures based on differences in molecular size. Small molecules can enter the internal pores of the gel particles and stay in the column for a long time, while large molecules are excluded from the outside of the particles and flow out quickly with the mobile phase. However, when faced with substances of similar molecular size, their retention behaviors in the gel column are similar, resulting in overlapping elution peaks and poor separation effects. At the same time, high-viscosity samples have poor fluidity and diffuse slowly in the column, and are easily attached to the surface of the gel particles, hindering the mass transfer of other molecules. Therefore, it is necessary to provide an industrial dextran gel column rapid chromatography equipment that can fully and evenly contact the gel particles and significantly improve the separation effect. Utility Model Content

[0004] The purpose of the utility model is to provide an industrial dextran gel column rapid chromatography device to solve the problems raised in the above background technology. To solve the above technical problems, the utility model is implemented through the following technical solutions:

[0005] The utility model is an industrial dextran gel column rapid chromatography device, comprising:

[0006] Equipment housing;

[0007] The diverter component includes support ring one, support ring two, support ring three, a diverter plate, a vertical pole, a support block, a rotating rod and a rotating shaft. The support ring one, support ring two and support ring three are respectively installed on the inner surface of the equipment casing, the vertical pole is fixed on the top of support ring two and support ring three, the rotating rod is located on the upper surface of the vertical pole, the diverter plate is respectively located on the upper surface of support ring two and support ring three, the support block is fixed on one end of the top of the diverter plate, the rotating shaft is arranged at both ends of the support block, and rotates on the outside of the rotating rod.

[0008] Furthermore, the diversion component also includes a vertical plate, and the vertical plate is fixed between the support ring one, the support ring two and the support ring three.

[0009] Furthermore, the support ring 1, the support ring 2 and the support ring 3 are detachably connected with filter screens, and the mesh sizes of the filter screens are different.

[0010] Furthermore, it also includes a fixed component, which includes a support plate, a movable groove, a movable rod and a support frame, the support frame is fixed between the rotating rod and the vertical pole, the support plate is fixed on the top of the vertical pole, the movable groove is opened inside the support plate, the movable rod is fixed on the outer surface of the support block, and is movably connected inside the movable groove.

[0011] Furthermore, a nut is threadedly connected to the outer surface of the moving rod, and the nut is fitted on the outer surface of the supporting plate.

[0012] Furthermore, it also includes an anti-sticking component, which includes an anti-sticking plate and a diversion groove. The anti-sticking plate is installed on the top of the diversion plate, and the diversion groove is opened on the top of the anti-sticking plate.

[0013] Furthermore, a fixing block is fixed on the outer wall of the diverter plate, and a slot is opened inside. An insert plate is fixed on the outer wall of the anti-sticking plate, and the insert plate is inserted and connected inside the slot.

[0014] The utility model has the following beneficial effects:

[0015] The utility model provides a diverter plate, a rotating rod and a rotating shaft between support ring one and support ring two, and between support ring two and support ring three, respectively. Large and small particles mixed in the sample can be filtered through the filter screens in support ring one, support ring two and support ring three, thereby improving the separation ability of substances with similar molecular sizes. The diverter plate can significantly optimize the processing of viscous samples by industrial dextran gel column rapid chromatography equipment. When the viscous sample enters the filter screen in support ring one, it will be quickly transferred to the diverter plate. At this time, the inclination angle of the diverter plate can be flexibly adjusted according to the specific viscosity characteristics and real-time flow conditions of the sample. The diverter plate rotates on the outside of the rotating rod through the rotating shaft, thereby avoiding the problem of blockage at the filter screen due to excessive viscosity of the sample. On the other hand, a more uniform sample distribution enables it to fully and evenly contact with the gel particles in the subsequent gel column separation process, thereby significantly improving the separation effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0017] Figure 1 It is an overall schematic diagram of the housing of the utility model device;

[0018] Figure 2 This is a schematic diagram of the interior of the housing of the utility model device;

[0019] Figure 3 This is a schematic diagram of support ring 1, support ring 2 and support ring 3 of the utility model;

[0020] Figure 4 For this utility model Figure 3 A magnified schematic diagram of the middle part;

[0021] Figure 5 This is a schematic diagram of the diverter plate of the utility model;

[0022] Figure 6 It is a schematic diagram of the anti-sticking plate of the utility model.

[0023] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0024] 100. Equipment casing;

[0025] 200, support ring 1; 201, support ring 2; 202, support ring 3; 203, filter screen; 204, vertical plate; 205, diverter plate; 206, vertical rod; 207, support block; 208, rotating rod; 209, rotating shaft;

[0026] 300, support plate; 301, moving groove; 302, moving rod; 303, nut; 304, support frame;

[0027] 400, anti-sticking plate; 401, diverter slot; 402, fixing block; 403, slot; 404, plug-in plate. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0029] In order to make the purpose, technical solution and advantages of the present invention more clear, the implementation mode of the present invention will be further described in detail below with reference to the accompanying drawings.

[0030] See also Figure 1-4 As shown, the utility model is an industrial dextran gel column rapid chromatography device, comprising:

[0031] Device housing 100;

[0032] A flow dividing component, wherein the flow dividing component comprises a support ring 1 200, a support ring 201, a support ring 3 202, a flow dividing plate 205, a vertical rod 206, a support block 207, a rotating rod 208 and a rotating shaft 209. The support ring 1 200, the support ring 201 and the support ring 3 202 are respectively mounted on the inner surface of the equipment housing 100, the vertical rod 206 is fixed on the top of the support ring 201 and the support ring 3 202, the rotating rod 208 is located on the upper surface of the vertical rod 206, the flow dividing plate 205 is respectively located on the upper surface of the support ring 201 and the support ring 3 202, the support block 207 is fixed on one end of the top of the flow dividing plate 205, and the rotating shaft 209 is arranged at both ends of the support block 207 and rotates outside the rotating rod 208;

[0033] Large and small particles mixed in the sample can be filtered through the filter 203 in the support ring 1 200, the support ring 201 and the support ring 3 202, so as to improve the separation ability of substances with similar molecular sizes. The diverter plate 205 can significantly optimize the processing of viscous samples by the industrial dextran gel column rapid chromatography equipment. When the viscous sample enters the filter 203 in the support ring 1 200, it will be quickly transferred to the diverter plate 205.

[0034] The flow diversion component further includes a vertical plate 204 , and the vertical plate 204 is fixed between the support ring 1 200 , the support ring 201 , and the support ring 3 202 .

[0035] The support ring 1 200, the support ring 201 and the support ring 3 202 are detachably connected with the filter screen 203, and the meshes of the filter screens 203 are different;

[0036] The filter holes of the filter screens 203 inside the support ring 1 200 , the support ring 2 201 and the support ring 3 202 are all different, and samples of different sizes can be filtered respectively.

[0037] It also includes a fixed component, which includes a support plate 300, a moving groove 301, a moving rod 302 and a support frame 304. The support frame 304 is fixed between the rotating rod 208 and the vertical rod 206. The support plate 300 is fixed on the top of the vertical rod 206. The moving groove 301 is opened inside the support plate 300. The moving rod 302 is fixed on the outer surface of the support block 207 and is movably connected inside the moving groove 301.

[0038] When the splitter plate 205 rotates, the moving rod 302 simultaneously moves inside the moving groove 301 , and then the nut 303 is fixed to the outside of the moving rod 302 , so that the angle of the splitter plate 205 after rotation can be fixed.

[0039] The outer surface of the moving rod 302 is threadedly connected with a nut 303 , and the nut 303 is attached to the outer surface of the supporting plate 300 .

[0040] Working principle: first, the large and small particles mixed in the sample can be filtered through the filter 203 in the support ring 1 200, the support ring 201 and the support ring 3 202, and the separation ability of substances with similar molecular sizes can be significantly optimized. The splitter plate 205 can significantly optimize the processing of viscous samples for the industrial dextran gel column rapid chromatography equipment. The splitter plate 205 rotates on the outside of the rotating rod 208 through the rotating shaft 209. As the splitter plate 205 rotates, the moving rod 302 moves inside the moving groove 301 at the same time. Then, the nut 303 is tightened on the moving rod. The outside of 302 fixes the adjusted angle of the diverter plate 205. When the viscous sample enters the filter screen 203 in the support ring 200, it will be quickly transferred to the diverter plate 205. At this time, the inclination angle of the diverter plate 205 can be flexibly adjusted according to the specific viscosity characteristics and real-time flow conditions of the sample, thereby avoiding the problem of blockage at the filter screen 203 due to excessive viscosity of the sample. On the other hand, a more uniform distribution of the sample enables it to fully and evenly contact with the gel particles in the subsequent gel column separation process, thereby significantly improving the separation effect.

[0041] See also Figure 1 , Figure 2 , Figure 5 , Figure 6 As shown, this embodiment is based on the above embodiment and also includes:

[0042] An anti-sticking component, the anti-sticking component includes an anti-sticking plate 400 and a diverter groove 401, the anti-sticking plate 400 is installed on the top of the diverter plate 205, and the diverter groove 401 is opened on the top of the anti-sticking plate 400;

[0043] An anti-sticking plate 400 is installed outside the diverter plate 205 . When samples with different characteristics, especially high-viscosity samples, flow through the diverter plate 205 , the anti-sticking material plays a vital role.

[0044] A fixing block 402 is fixed on the outer wall of the diverter plate 205, and a slot 403 is opened inside. An insert plate 404 is fixed on the outer wall of the anti-sticking plate 400, and the insert plate 404 is inserted and connected inside the slot 403;

[0045] The insert plate 404 is inserted into the interior of the slot 403 so that the anti-sticking plate 400 is installed on the top of the diverter plate 205 .

[0046] Working principle: first, an anti-sticking plate 400 is installed on the outside of the diverter plate 205. When samples with different characteristics, especially high-viscosity samples, flow through the diverter plate 205, the anti-sticking material plays a vital role. The plug plate 404 can be inserted into the inside of the slot 403, so that the anti-sticking plate 400 is installed on the top of the diverter plate 205, which can effectively reduce the adhesion between the sample and the surface of the diverter plate 205. When the high-viscosity sample contacts the surface of the diverter plate 205, it will not easily stick to the surface of ordinary materials. This means that the sample can flow more smoothly along the surface of the diverter plate 205, avoiding the local accumulation problem caused by sample adhesion.

[0047] The preferred embodiments of the utility model disclosed above are only used to help explain the utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the utility model to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that technicians in the relevant technical field can well understand and use the utility model. The utility model is limited only by the claims and their full scope and equivalents.

Claims

1. An industrial dextran gel column rapid chromatography device, characterized in that: include: Equipment housing (100); A flow diversion component, the flow diversion component comprising a support ring 1 (200), a support ring 2 (201), a support ring 3 (202), a diversion plate (205), a vertical rod (206), a support block (207), a rotating rod (208) and a rotating shaft (209), wherein the support ring 1 (200), the support ring 2 (201) and the support ring 3 (202) are respectively mounted on the inner surface of the equipment housing (100), the vertical rod (206) is fixed on the top of the support ring 2 (201) and the support ring 3 (202), the rotating rod (208) is located on the upper surface of the vertical rod (206), the diversion plate (205) is respectively located on the upper surface of the support ring 2 (201) and the support ring 3 (202), the support block (207) is fixed on one end of the top of the diversion plate (205), and the rotating shaft (209) is arranged at both ends of the support block (207) and rotates outside the rotating rod (208).

2. An industrial dextran gel column rapid chromatography equipment according to claim 1, characterized in that: The flow distribution component further comprises a vertical plate (204), wherein the vertical plate (204) is fixed between the first support ring (200), the second support ring (201) and the third support ring (202).

3. An industrial dextran gel column rapid chromatography equipment according to claim 1, characterized in that: The support ring 1 (200), the support ring 2 (201) and the support ring 3 (202) are detachably connected with a filter screen (203), and the mesh openings of the filter screens (203) are different.

4. The industrial dextran gel column rapid chromatography equipment according to claim 1, characterized in that: The invention also comprises a fixing component, wherein the fixing component comprises a support plate (300), a movable groove (301), a movable rod (302) and a support frame (304); the support frame (304) is fixed between the rotating rod (208) and the vertical rod (206); the support plate (300) is fixed on the top of the vertical rod (206); the movable groove (301) is provided inside the support plate (300); the movable rod (302) is fixed on the outer surface of the support block (207) and is movably connected inside the movable groove (301).

5. An industrial dextran gel column rapid chromatography equipment according to claim 4, characterized in that: The outer surface of the moving rod (302) is threadedly connected to a nut (303), and the nut (303) is fitted on the outer surface of the supporting plate (300).

6. The industrial dextran gel column rapid chromatography equipment according to claim 1, characterized in that: It also includes an anti-sticking component, which includes an anti-sticking plate (400) and a diversion groove (401). The anti-sticking plate (400) is installed on the top of the diversion plate (205), and the diversion groove (401) is opened on the top of the anti-sticking plate (400).

7. An industrial dextran gel column rapid chromatography equipment according to claim 6, characterized in that: A fixing block (402) is fixed on the outer wall of the diverter plate (205), and a slot (403) is provided inside. An insert plate (404) is fixed on the outer wall of the anti-sticking plate (400), and the insert plate (404) is inserted and connected inside the slot (403).

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