Homogenizing mechanism applicable to environmental DNA enricher

Abstract

The utility model relates to the technical field of environmental monitoring, in particular to a homogenizing mechanism applicable to environmental DNA enrichers, which comprises an adapter used for being connected with a vortex grinding device and also used for placing a plurality of environmental DNA enrichers; the gland is detachably connected with the adapter, and the gland is used for fixing a plurality of environmental DNA enrichers placed on the adapter. A plurality of environmental DNA enrichers can be installed through the adapter, the enrichers can be pressed and fixed through the gland, and the homogenizing mechanism and the vortex grinding device can act synchronously after being connected, so that the homogenizing and grinding functions are realized.

Description

Technical Field

[0001] The utility model relates to the technical field of environmental monitoring, in particular to a homogenizing mechanism suitable for an environmental DNA enricher. Background Art

[0002] Environmental DNA, or eDNA, refers to a method of directly sampling DNA fragments from environmental samples (soil, sediment, air, water, etc.) and then using sequencing technology for qualitative or quantitative analysis. In recent years, with the development of molecular biology, eDNA technology has become a new method for aquatic biological surveys, mainly used for detection and prevention of biological invasions, protection of endangered species, evaluation of biodiversity and biomass, real-time species distribution and community diversity information, tracking of species life history processes, and reflecting changes in ecosystem structure.

[0003] For example, Chinese patent publication number CN220270869U discloses an eDNA collection and analysis system. In this prior art, an indoor collector, an outdoor collector, a buoy collector and a float collector are respectively provided with an integrated filter for filtering and collecting eDNA, and this filter is an enricher.

[0004] The enricher that retains environmental DNA needs to be connected to a vortex grinding device to achieve the homogenization function of the environmental DNA enricher. However, in the prior art, there is a lack of a mechanism that can connect multiple environmental DNA enrichers to a vortex grinding device to achieve simultaneous homogenization and grinding of multiple environmental DNA enrichers. Utility Model Content

[0005] In order to solve the above problems, the purpose of the utility model is to provide a homogenizing mechanism suitable for an environmental DNA enricher.

[0006] The technical solution provided by the utility model is:

[0007] A homogenizing mechanism suitable for an environmental DNA enricher, comprising:

[0008] An adapter, the adapter is used to connect to the vortex grinding device, and the adapter is also used to place multiple environmental DNA enrichers;

[0009] A pressure cover is detachably connected to the adapter and is used to fix a plurality of environmental DNA enrichers placed on the adapter.

[0010] As an optional technical solution, the adapter is provided with a central through hole, and a connecting seat is provided in the central through hole;

[0011] in,

[0012] The adapter and the connecting seat are used to connect with the vortex grinding device;

[0013] The pressure cover is detachably connected to the connection seat.

[0014] Optionally, the connection seat is provided with a stud; the gland is provided with a through hole corresponding to the stud;

[0015] Also includes a screw cap, the screw cap is provided with an internal thread;

[0016] When the end of the stud extends out of the through hole and the screw cap is screwed on the stud, the screw cap presses and fixes the multiple environmental DNA enrichers placed on the adapter.

[0017] Optionally, the gland is provided with a hollow boss, and the through hole is provided on the upper end surface of the hollow boss;

[0018] In the vertical direction, the height of the upper end surface of the hollow boss is higher than the multiple environmental DNA enrichers placed on the adapter.

[0019] Optionally, the screw cap is provided with a convex tube, and the internal thread is provided with a tube wall of the convex tube;

[0020] The convex tube passes through the through hole and extends into the hollow boss. A bayonet is installed on the outer wall of the convex tube located in the hollow boss. The bayonet is used to prevent the rotary cover from falling off the hollow boss.

[0021] As an optional technical solution, the adapter is provided with an annular bevel edge, and the bevel edge is provided with a plurality of holes in an annular shape, and the holes are used to accommodate the lower nut of the environmental DNA enricher to be inserted;

[0022] The pressure cover is provided with a pressure plate corresponding to the insertion hole, and the pressure plate is provided with a strip hole, and the strip hole is used for accommodating the upper nut of the environmental DNA enricher to be inserted.

[0023] Optionally, the bevel edge is further provided with a limiting groove corresponding to the insertion hole.

[0024] Optionally, the bevel edge is also provided with a pair of limiting flanges corresponding to the insertion hole, and the pair of limiting flanges are respectively located on both sides of the hole end of the insertion hole;

[0025] When the lower nut of the environmental DNA enricher is inserted into the insertion hole, the limiting flange abuts against the outer wall of the environmental DNA enricher.

[0026] Optionally, the adapter is provided with a lower limit piece; the gland is provided with an upper limit piece matched with the lower limit piece;

[0027] When the gland is connected to the adapter, the upper limit member is connected to the lower limit member to prevent the gland from rotating relative to the adapter.

[0028] Furthermore, the lower limiter is annular and coaxial with the central through hole, and an annular tooth is provided on one end of the lower limiter facing the gland;

[0029] The upper limit member is also provided with annular teeth at one end facing the adapter.

[0030] Compared with the prior art, the technical solution provided by the utility model has the following beneficial effects:

[0031] The utility model can install a plurality of environmental DNA enrichers through the adapter, and can press and fix the enrichers through the pressure cover. After the homogenizing mechanism is connected with the vortex grinding device, they can act synchronously, thereby realizing the homogenizing and grinding function.

[0032] The pressure cover in the utility model can be disassembled, that is, the adapter can be kept connected to the vortex grinding device. Only the pressure cover is removed, and the processed environmental DNA enricher is taken out and replaced with a new environmental DNA enricher to be processed. There is no need to frequently disassemble and assemble the adapter and the vortex grinding device, making the homogenization mechanism easier to use.

[0033] The adapter of the utility model is provided with an annular bevel edge, and the environmental DNA enricher is in an inclined state when being fixed, so that the filter membrane in the enricher can be fully ground and homogenized under the action of the grinding beads. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is a three-dimensional diagram of a homogenizing mechanism in one embodiment of the present application;

[0035] Figure 2 This is a side view of a homogenizing mechanism in one embodiment of the present application;

[0036] Figure 3 This is a schematic diagram of a through hole provided in an adapter in one embodiment of the present application;

[0037] Figure 4 A schematic diagram of a stud provided on a connecting seat in an embodiment of the present application;

[0038] Figure 5 This is a schematic diagram of a limiting flange provided on an adapter in one embodiment of the present application;

[0039] Figure 6 This is a schematic diagram of the structure of a lower limit member in an embodiment of the present application;

[0040] Figure 7 This is a schematic diagram of the structure of the upper limit member provided on the gland in one embodiment of the present application;

[0041] Figure 8 A top view of a gland in one embodiment of the present application;

[0042] Fig. 9 This is a schematic diagram of the connection between the pressing cover and the rotating cover in one embodiment of the present application;

[0043] Fig.10This is a schematic diagram of a screw cap structure in one embodiment of the present application.

[0044] Explanation of the symbols in the schematic diagram:

[0045] Environmental DNA enricher 101, upper nut 102, lower nut 103;

[0046] Adapter 201, middle through hole 202, connecting seat 203, stud 204, lower limiting member 205, limiting flange 206, limiting groove 207, plug hole 208;

[0047] Pressure cover 301, pressure plate 302, strip-shaped hole 303, hollow boss 304, upper limit member 305;

[0048] Screw cap 401 , internal thread 402 , and bayonet pin 403 . DETAILED DESCRIPTION

[0049] In order to further understand the content of the utility model, the utility model is described in detail in conjunction with the drawings and embodiments.

[0050] The structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention, so they have no substantial technical significance. Any modification of the structure, change of the proportion relationship or adjustment of the size, without affecting the effects and purposes that can be achieved by the present invention, should still fall within the scope of the technical content disclosed by the present utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and so on quoted in this specification are only for the convenience of description, and are not used to limit the scope of implementation. The change or adjustment of their relative relationship should also be regarded as the scope of implementation of the present invention without substantially changing the technical content.

[0051] In one embodiment, if Figure 1 As shown, the utility model proposes a homogenizing mechanism suitable for environmental DNA enrichers, including an adapter 201 and a pressure cover 301, wherein the adapter 201 can be connected to a vortex grinding device, and the vortex grinding device is relatively mature in the prior art and will not be described in detail here. A plurality of environmental DNA enrichers 101 can be placed on the adapter 201, and then the plurality of environmental DNA enrichers 101 placed on the adapter 201 can be fixed by the pressure cover 301. When the vortex grinding device is connected to drive the homogenizing mechanism for high-speed vortex grinding, the enricher fixed by the pressure cover 301 will not fall off.

[0052] In order to facilitate the replacement of other environmental DNA enrichers to be processed after the environmental DNA enricher is processed, in one embodiment, the pressure cap 301 can be detachably connected to the adapter 201, that is, after the environmental DNA enricher in the homogenizing mechanism is processed, the adapter is kept connected to the vortex grinding device, the pressure cap is removed, and the processed environmental DNA enricher is taken out, and then replaced with a new environmental DNA enricher to be processed. In this way, there is no need to frequently disassemble and assemble the adapter and the vortex grinding device, making the homogenizing mechanism easier to use.

[0053] In one embodiment, the adapter 201 is provided with a central through hole 202, wherein a connection seat 203 is provided in the through hole 202. The adapter 201 and the connection seat 203 are both used to connect with the vortex grinding device. In this technical solution, the gland 301 is detachably connected with the connection seat 203. By fixing the adapter 201 on the vortex grinding device with screws, and fixing the connection seat 203 on the vortex grinding device with screws, the environmental DNA enricher samples are symmetrically placed on the adapter in sequence, and then the gland 301 is installed. The vortex grinding device is started, and the entire homogenizing mechanism is synchronously operated with the vortex grinding device to achieve the homogenizing and grinding function.

[0054] The environmental DNA enricher is placed symmetrically on the adapter, so that when the vortex grinding device drives the homogenizing mechanism to move, the homogenizing mechanism will move more stably and avoid shaking.

[0055] Specifically, the connecting seat 203 is provided with a stud 204, and the pressure cover 301 is provided with a through hole corresponding to the stud 204. After the pressure cover 301 is installed, the stud 204 is extended from the through hole, and then a screw cap 401 with an internal thread 402 is used to be installed on the stud 204, and the screw cap 401 is tightened on the stud 204. The pressure cover 301 can press and fix multiple environmental DNA enrichers 101 placed on the adapter 201.

[0056] In order to facilitate personnel to tighten or loosen the screw cap 401, in one embodiment, the gland 301 is provided with a hollow boss 304. The boss is hollow, which can reduce the weight of the gland 301 and facilitate the extension of the stud 204. The through hole is provided on the upper end surface of the hollow boss 304, so that the screw cap 401 is located at the upper end of the hollow boss 304, which is convenient for personnel to operate.

[0057] In the vertical direction, the height of the upper end surface of the hollow boss 304 is higher than the multiple environmental DNA enrichers 101 placed on the adapter 201 , so that when a person rotates the rotary cover 401 , the environmental DNA enrichers 101 will not be touched.

[0058] In order to connect the screw cap 401 with the gland 301, the screw cap 401 will not separate from the gland 301 even after being loosened. The screw cap 401 is provided with a convex tube, and the internal thread 402 is provided with a tube wall of the convex tube. The convex tube passes through the through hole and extends into the hollow boss 304. The outer wall of the convex tube located in the hollow boss 304 is provided with a bayonet 403, and the outer diameter of the bayonet 403 is larger than the inner diameter of the through hole, so that the screw cap 401 can rotate relative to the hollow boss 304, and can also move vertically within a certain range relative to the hollow boss 304, but it can prevent the screw cap 401 from falling off the hollow boss 304.

[0059] In one embodiment, the adapter 201 is provided with an annular bevel edge, and the bevel edge is provided with a plurality of insertion holes 208 in an annular shape, and the insertion holes 208 are used to accommodate the lower nut 103 of the environmental DNA enricher 101. A pressing plate 302 corresponding to the insertion hole 208 is provided on the pressure cover 301, and a strip hole 303 is provided on the pressing plate 302, and the strip hole 303 is used to accommodate the upper nut 102 of the environmental DNA enricher 101. When installing the environmental DNA enricher 101, the lower nut 103 is first inserted into the insertion hole 208, and the environmental DNA enricher 101 is in an inclined state at this time, and then the pressure cover 301 is installed. Since the pressing plate 302 is provided with a strip hole 303, the inclined upper nut 102 will be easier to insert into the strip hole 303.

[0060] The inclination angle of the annular bevel edge can be 45 degrees, so that the filter membrane in the enricher can be fully ground and homogenized under the action of the grinding beads.

[0061] In order to improve the fixing effect of the environmental DNA enricher 101, a limiting groove 207 corresponding to the insertion hole 208 is further provided on the oblique edge. The limiting groove 207 is adapted to the shape of the lower end surface of the enricher, so that the enricher can be placed on the adapter 201 in an inclined posture.

[0062] The bevel edge is also provided with a pair of limiting flanges 206 corresponding to the insertion hole 208, and the pair of limiting flanges 206 are respectively located on both sides of the hole end of the insertion hole 208. When the lower nut 103 of the environmental DNA enricher 101 is inserted into the insertion hole 208, the limiting flanges 206 abut against the outer wall of the environmental DNA enricher 101, thereby preventing the enricher from lateral displacement.

[0063] In order to improve the docking accuracy of the gland 301 and the adapter 201, the pressing plate 302 corresponds to the enricher one by one, and to prevent the gland 301 from rotating relative to the adapter 201 when the vortex grinding device drives the homogenizing mechanism to move. In this embodiment, the adapter 201 is provided with a lower stopper 205, and the gland 301 is provided with an upper stopper 305 adapted to the lower stopper 205. When the gland 301 is connected to the adapter 201, the upper stopper 305 is connected to the lower stopper 205 to prevent the gland 301 from rotating relative to the adapter 201, and at the same time, the pressing plate 302 corresponds to the enricher one by one.

[0064] Specifically, the lower limit member 205 is annular and coaxial with the middle through hole 202. The lower limit member 205 is provided with annular teeth at one end facing the gland 301, and the upper limit member 305 is also provided with annular teeth at one end facing the adapter 201. The annular teeth of the upper limit member 305 are butted and meshed with the annular teeth of the lower limit member 205, so that the gland 301 can be limited to rotate relative to the adapter 201, and the pressing plate 302 can also correspond to the enricher one by one. As an optional solution, the lower limit member 205 can also be detachably connected to the adapter 201, for example, the two can be connected together by screws, so that when the lower limit member 205 is damaged, it can also be replaced. Similarly, the upper limit member 305 can also be detachably connected to the gland 301, so that when the upper limit member 305 is damaged, it can also be replaced.

[0065] The above schematically describes the present invention and its implementation methods, which are not restrictive. The drawings show only one implementation method of the present invention, and the actual structure is not limited thereto. Therefore, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the present invention, they should all fall within the protection scope of the present invention.

Claims

1. A homogenizing mechanism suitable for an environmental DNA enricher, characterized in that: include: An adapter (201), the adapter (201) is used to connect to a vortex grinding device, and the adapter (201) is also used to place a plurality of environmental DNA enrichers (101); A pressure cover (301) is detachably connected to the adapter (201), and the pressure cover (301) is used to fix a plurality of environmental DNA enrichers (101) placed on the adapter (201).

2. The homogenizing mechanism according to claim 1, characterized in that: The adapter (201) is provided with a central through hole (202) in the center, and a connecting seat (203) is provided in the central through hole (202); in, The adapter (201) and the connection seat (203) are both used to connect to the vortex grinding device; The pressure cover (301) is detachably connected to the connection seat (203).

3. The homogenizing mechanism according to claim 2, characterized in that: The connection seat (203) is provided with a stud (204); the gland (301) is provided with a through hole corresponding to the stud (204); It also includes a screw cap (401), wherein the screw cap (401) is provided with an internal thread (402); When the end of the stud (204) extends out of the through hole and the screw cap (401) is screwed on the stud (204), the press cap (301) presses and fixes the multiple environmental DNA enrichers (101) placed on the adapter (201).

4. The homogenizing mechanism according to claim 3, characterized in that: The gland (301) is provided with a hollow boss (304), and the through hole is provided on the upper end surface of the hollow boss (304); In the vertical direction, the height of the upper end surface of the hollow boss (304) is higher than the multiple environmental DNA enrichers (101) placed on the adapter (201).

5. The homogenizing mechanism according to claim 4, characterized in that: The screw cap (401) is provided with a convex tube, and the internal thread (402) is provided with a tube wall of the convex tube; The convex tube passes through the through hole and extends into the hollow boss (304). A latch (403) is installed on the outer wall of the convex tube located in the hollow boss (304). The latch (403) is used to prevent the rotary cover (401) from falling off the hollow boss (304).

6. The homogenizing mechanism according to claim 1, characterized in that: The adapter (201) is provided with an annular bevel edge, and the bevel edge is provided with a plurality of insertion holes (208) in an annular shape, and the insertion holes (208) are used to accommodate the lower nut (103) of the environmental DNA enricher (101) to be inserted; The pressure cover (301) is provided with a pressure plate (302) corresponding to the insertion hole (208), and the pressure plate (302) is provided with a strip hole (303), and the strip hole (303) is used to accommodate the upper nut (102) of the environmental DNA enricher (101) to be inserted.

7. The homogenizing mechanism according to claim 6, characterized in that: The oblique edge is also provided with a limiting groove (207) corresponding to the insertion hole (208).

8. The homogenizing mechanism according to claim 6, characterized in that: The bevel edge is also provided with a pair of limiting flanges (206) corresponding to the insertion hole (208), and the pair of limiting flanges (206) are respectively located on both sides of the hole end of the insertion hole (208); When the lower nut (103) of the environmental DNA enricher (101) is inserted into the insertion hole (208), the limiting flange (206) abuts against the outer wall of the environmental DNA enricher (101).

9. The homogenizing mechanism according to claim 2, characterized in that: The adapter (201) is provided with a lower limit member (205); the gland (301) is provided with an upper limit member (305) adapted to the lower limit member (205); When the gland (301) is connected to the adapter (201), the upper limit member (305) is connected to the lower limit member (205) to prevent the gland (301) from rotating relative to the adapter (201).

10. The homogenizing mechanism according to claim 9, characterized in that: The lower limit member (205) is annular and coaxial with the central through hole (202), and an annular tooth is provided on one end of the lower limit member (205) facing the gland (301); The upper limit member (305) is also provided with annular teeth at one end facing the adapter (201).

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