Centrifugal column method nucleic acid extraction device

Abstract

The utility model provides a nucleic acid extraction device adopting a centrifugal column method. The nucleic acid extraction device comprises a centrifugal machine and a centrifugal column type kit, the centrifugal machine comprises a machine body, a machine cover and a rotary drum, a centrifugal cavity is formed in the middle of the top surface of the machine body, the rotary drum is mounted in the centrifugal cavity, the centrifugal column type kit comprises an adsorption column and a collecting pipe, and a plurality of mounting holes for obliquely inserting the collecting pipe are formed in the top surface of the rotary drum in a circumferential array manner; a liquid leakage hole is formed in the bottom surface of the centrifugal cavity, a waste liquid pipe is connected to the bottom of the liquid leakage hole, and the other end of the waste liquid pipe extends out of the machine body and is connected with a waste liquid bottle; the opening, the mounting hole, the through hole, the centrifugal cavity, the liquid leakage hole and the waste liquid pipe jointly form a waste liquid drainage channel, so that waste liquid generated by the adsorption column is drained into a waste liquid bottle through the drainage channel; in the nucleic acid extraction process, the operation step of pouring waste liquid for many times can be omitted, the working efficiency is high, and the working intensity of operators is also relieved.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction, in particular to a centrifugal column method nucleic acid extraction device. Background Art

[0002] Nucleic acid extraction refers to the separation and extraction of DNA or RNA molecules from complex biological samples to obtain pure nucleic acid samples. Nucleic acid extraction is a basic component of molecular biology, and the application of high-quality nucleic acids in molecular biology is crucial; centrifugal column method nucleic acid extraction is a relatively common nucleic acid extraction method. The main equipment used includes a centrifuge and a centrifugal column kit. The centrifugal column kit includes a collection tube and an adsorption column sleeved in the collection tube. The centrifugal column method nucleic acid extraction is mainly performed by injecting the biological sample into the adsorption column, and placing the centrifugal column kit in a centrifuge for centrifugation. The adsorption membrane on the adsorption column specifically adsorbs the DNA in the sample, while non-target substances can be eluted and removed by centrifugation with different reagents, and finally dissolved and collected to obtain the DNA adsorbed on the membrane; as shown in the attached manual Figure 1 This is an extraction step for centrifugal column method of nucleic acid extraction.

[0003] According to the instruction manual Figure 1 It can be seen from the extraction steps in that after the mixed liquid is transferred into the adsorption column for centrifugation in step 5, the waste liquid will be collected by the collection tube. When performing subsequent operation steps 6-9, the waste liquid in the collection tube in the previous step needs to be poured out before proceeding to the next step. Therefore, the operation of dumping the waste liquid needs to be repeated many times during the extraction process, which is not only time-consuming and labor-intensive, and has low work efficiency, but also increases the work intensity of the operator, especially when the sample size of the nucleic acid extraction is large, it will be more obvious. Summary of the invention

[0004] The utility model aims to provide a centrifugal column method nucleic acid extraction device which can save multiple waste liquid dumping operation steps in the nucleic acid extraction process, so as to solve the problems existing in the background technology.

[0005] The utility model adopts the following technical scheme: a centrifugal column method nucleic acid extraction device, comprising a centrifuge and a centrifugal column type reagent kit; the centrifuge comprises a body, a cover and a drum, a centrifugal chamber is arranged in the middle of the top surface of the body, the drum is installed in the centrifugal chamber, a motor is arranged in the body to indirectly or directly drive the drum to rotate, the centrifugal column type reagent kit comprises an adsorption column and a collection tube, the adsorption column is detachably inserted into the collection tube through the top opening of the collection tube, the drum is in the shape of a disc as a whole, and a plurality of mounting holes for the collection tube to be obliquely inserted are arranged in a circular array on the top surface of the drum;

[0006] An opening is provided at the bottom of the collection tube. A through hole extending out of the side or bottom of the drum is provided at the bottom of the mounting hole. A liquid leakage hole is provided on the bottom surface of the centrifugal chamber. A waste liquid pipe is connected to the bottom of the liquid leakage hole. The other end of the waste liquid pipe extends out of the machine body and is connected to a waste liquid bottle. The opening, the mounting hole, the through hole, the centrifugal chamber, the liquid leakage hole and the waste liquid pipe together form a drainage channel for the waste liquid, so that the waste liquid generated by the adsorption column is drained into the waste liquid bottle through the drainage channel.

[0007] Further, the centrifuge further includes a protective cover. The protective cover includes an annular top plate and side plates vertically installed on the outer edge of the top plate. A sleeve hole for exposing the top surface of the drum is provided in the middle of the top plate. The protective cover is fixedly connected to the side wall of the centrifugal chamber through the side plates. At least one handle is provided on the top surface of the top plate.

[0008] Further, the bottom end of the side plate is a snap ring with a circular cross-section. The diameter of the circular cross-section of the snap ring is greater than the thickness of the side plate, and the outer diameter of the snap ring is greater than or equal to the inner diameter of the centrifugal chamber. The protective cover is fixedly connected to the side wall of the centrifugal chamber through the snap ring at the bottom end of the side plate.

[0009] Further, a rotating shaft is provided in the middle of the centrifugal chamber. The rotating shaft is indirectly or directly driven to rotate by a motor arranged in the machine body. A rotating shaft pin is fixedly and vertically arranged on the rotating shaft. A hollow shaft sleeve is provided in the middle of the drum. A clamping groove is provided on the bottom surface of the shaft sleeve. The rotating shaft and the shaft sleeve are fixedly sleeved, and the rotating shaft pin is matched and clamped in the clamping groove.

[0010] Preferably, the waste liquid pipe is an inclined pipe. The end connected to the liquid leakage hole is the high end of the inclined pipe, and the end connected to the waste liquid bottle is the low end of the inclined pipe.

[0011] Further, the adsorption column includes a hollow column body and an end cover arranged at the top of the column body. The end cover is used to seal the top opening of the column body. The bottom end of the column body is a conical structure. A convex column is arranged on the inner side of the conical structure. An adsorption film is arranged on the convex column. The adsorption film is fixed on the convex column through a fixing ring. A plurality of vertically arranged clamping strips are arranged in a circumferential array on the upper part of the outer side of the column body;

[0012] A clamping ring is horizontally arranged on the inner side of the top opening of the collection tube. The adsorption column is detachably inserted into the collection tube through the top opening of the collection tube, and is clamped and fixed through the clamping strips and the clamping ring.

[0013] Further, extend the length of the part of the column body inserted into the collection tube, and / or extend the length of the part outside the collection tube to increase the total volume of the inner cavity of the column body.

[0014] The nucleic acid extraction device by the centrifugal column method in the present utility model is improved from the existing centrifuge and centrifugal column type reagent kit. An opening is provided at the bottom of the collection tube, a through hole extending out of the side or bottom of the drum is provided at the bottom of the mounting hole of the drum, a liquid leakage hole is provided at the bottom surface of the centrifugal chamber, a waste liquid pipe is connected to the bottom of the liquid leakage hole, and the other end of the waste liquid pipe extends out of the fuselage and is connected to a waste liquid bottle. The provided opening, mounting hole, through hole, centrifugal chamber, liquid leakage hole and waste liquid pipe together constitute a drainage channel for waste liquid, so that the waste liquid generated by the adsorption column can be directly drained into the waste liquid bottle through the drainage channel, which can save the operation steps of pouring waste liquid many times during nucleic acid extraction, with high working efficiency and reduced work intensity of the operator. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Attached Figure 1 is an extraction step of the nucleic acid extraction by the centrifugal column method.

[0016] Attached Figure 2 is a schematic structural view of the centrifuge in the present utility model Figure 1 .

[0017] Attached Figure 3 is a schematic structural view of the centrifuge in the present utility model Figure 2 .

[0018] Attached Figure 4 is a schematic structural view of the centrifuge in the present utility model after disassembling the drum 3 and the protective cover 4.

[0019] Attached Figure 5 is a schematic structural view of the drum 3 in the present utility model Figure 1 .

[0020] Attached Figure 6 is a schematic structural view of the drum 3 in the present utility model Figure 2 .

[0021] Attached Figure 7 is a schematic structural view of the protective cover 4 in the present utility model Figure 1 .

[0022] Attached Figure 8 is a schematic structural view of the protective cover 4 in the present utility model Figure 2 .

[0023] Attached Figure 9 is a partial sectional schematic view of the installation positions among the drum 3, the centrifugal chamber 11 and the protective cover 4 in the present utility model.

[0024] Attached Figure 10 is a schematic structural view of the first centrifugal column type reagent kit 7 in the present utility model.

[0025] Attached Figure 11 is a sectional structural schematic view of the first centrifugal column type reagent kit 7 in the present utility model.

[0026] Attached Figure 12 is a schematic structural view of the second centrifuge column type kit 7 in the present utility model.

[0027] Attached Figure 13 is a schematic sectional view of the second centrifuge column type kit 7 in the present utility model.

[0028] The description of the reference numerals is as follows: body 1, centrifugal chamber 11, rotating shaft 12, liquid leakage hole 13, machine cover 2, drum 3, shaft sleeve 31, mounting hole 32, through hole 33, clamping groove 34, protective cover 4, top plate 41, sleeve hole 411, side plate 42, snap ring 43, handle 44, waste liquid pipe 5, waste liquid bottle 6, centrifuge column type kit 7, adsorption column 71, column body 711, end cover 712, clamping strip 713, adsorption film 714, fixing ring 715, collection tube 72, clamping ring 721, opening 722. Detailed implementation manners

[0029] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings. The following further describes the content of the invention with reference to the drawings:

[0030] Embodiment 1

[0031] A nucleic acid extraction device by the centrifugal column method includes a centrifuge and a centrifuge column type kit 7.

[0032] Referring to the attached drawings of the specification Figure 2 、 3 、4, the centrifuge includes a body 1, a machine cover 2 and a drum 3. A centrifugal chamber 11 is provided in the middle of the top surface of the body 1. A liquid leakage hole 13 is opened at the bottom of the centrifugal chamber 11. A rotating shaft 12 is provided in the middle of the centrifugal chamber 11. Specifically, a bearing (preferably a waterproof bearing or a sealed bearing) is provided in the middle of the bottom side of the centrifugal chamber 11. The rotating shaft 12 is rotatably installed on the bearing. The rotating shaft 12 is indirectly or directly driven to rotate by a motor fixedly provided in the body 1. A rotating shaft pin is fixedly and vertically provided on the rotating shaft 12. One end of the machine cover 2 is hinged to the rear end of the body 1. When the machine cover 2 is closed, the centrifugal chamber 11 can be closed.

[0033] Referring to the attached drawings of the specification Figure 5 、 6, the drum 3 is integrally disc-shaped. On the top surface of the drum 3, a plurality of mounting holes 32 for the inclined insertion of the collection tubes 72 of the centrifugal column type reagent kit 7 are arranged in a circumferential array. Through holes 33 extending to the side or bottom of the drum 3 are provided at the bottoms of the mounting holes 32. A hollow sleeve 31 is arranged in the middle of the drum 3. A clamping groove 34 is provided on the bottom surface of the sleeve 31. The rotating shaft 12 is fixedly sleeved with the sleeve 31 (the rotating shaft 12 is sleeved into the inner cavity of the sleeve 31), and the rotating shaft pin is fitted and clamped in the clamping groove 34, so that the drum 3 is installed in the centrifugal chamber 11;

[0034] The centrifuge belongs to the commonly used and common instruments in the laboratory. The installation method and principle of its drum 3 should also be common knowledge. The following provides a specific installation method: Refer to the attached Figure 9 , the sleeve 31 is of a hollow or hollow structure. A convex ring is fixedly arranged in the upper part of the inner cavity of the sleeve 31. A threaded hole is provided on the top surface of the rotating shaft 12. When the sleeve 31 is sleeved on the rotating shaft 12 and the rotating shaft pin is fitted and clamped in the clamping groove 34, at this time, the top end of the rotating shaft 12 is located below the convex ring. The fixing screw (flat head or cross head or internal hexagon head) is placed from the top of the sleeve 31, passes through the convex ring, and then is screwed into the threaded hole through a screwdriver or an internal hexagon wrench until the nut of the fixing screw abuts against the top surface of the convex ring, that is, the fixed installation of the drum 3 on the rotating shaft 12 is completed.

[0035] Refer to the attached Figure 10 , 11 , the centrifugal column type reagent kit 7 includes an adsorption column 71 and a collection tube 72. The adsorption column 71 includes a hollow column body 711 and an end cap 712 arranged at the top of the column body 711. The end cap 712 is used to close the top opening of the column body 711. The bottom end of the column body 711 is of a conical structure. Convex columns are arranged on the inner side of the conical structure (the top surfaces of multiple convex columns form a plane). An adsorption membrane 714 is arranged on the convex columns. The adsorption membrane 714 is a silicon matrix filter membrane, which can selectively adsorb DNA or RNA fragments, while proteins and other impurities will not be adsorbed. The adsorption membrane 714 is fixed on the convex columns through a fixing ring 715. The middle of the fixing ring 715 is of a hollow structure, and its outer diameter is greater than or equal to the inner diameter of the column body 711. The fixing ring 715 can be clamped on the inner wall of the column body 711 (the outer diameter of the fixing ring 715 is greater than or equal to the inner diameter of the inner wall of the column body 711). A plurality of vertically arranged clamping strips 713 are arranged in a circumferential array on the upper part of the outer side of the column body 711;

[0036] A clamping ring 721 is horizontally arranged on the inner side of the top opening of the collection tube 72. The adsorption column 71 is detachably inserted into the collection tube 72 through the top opening of the collection tube 72, and is clamped and fixed through the clamping strips 713 and the clamping ring 721; An opening 722 is provided at the bottom of the collection tube 72.

[0037] A waste liquid pipe 5 is connected to the bottom of the liquid leakage hole 13. The other end of the waste liquid pipe 5 extends out of the fuselage 1 and is connected to a waste liquid bottle 6 outside the centrifuge. The waste liquid pipe 5 is an inclined pipe. The end connected to the liquid leakage hole 13 is the high end of the inclined pipe, and the end connected to the waste liquid bottle 6 is the low end of the inclined pipe. The waste liquid bottle 6 includes a bottle body and a bottle cap provided at the bottle mouth of the bottle body. A through hole is opened on the bottle cap or a connecting pipe is communicated. The waste liquid pipe 5 is inserted into the bottle body through the through hole on the bottle cap or the waste liquid pipe 5 is sleeved on the connecting pipe.

[0038] The above-mentioned opening 722, mounting hole 32, through hole 33, centrifugal chamber 11, liquid leakage hole 13 and waste liquid pipe 5 together form a drainage channel for waste liquid, so that the waste liquid generated by the centrifugal column type reagent kit 7 is drained to the waste liquid bottle 6 through the drainage channel, so that the waste liquid of the centrifugal column type reagent kit 7 is discharged and collected during the centrifugation process of the centrifuge, and the operation steps of pouring waste liquid multiple times can be omitted during the nucleic acid extraction process.

[0039] Embodiment 2

[0040] During the centrifugation process of the centrifuge of the centrifugal column type reagent kit 7, the waste liquid generated by the adsorption column 71 of the centrifugal column type reagent kit 7 sequentially enters the centrifugal chamber 11 through the opening 722 and the through hole 33. Since the centrifugal column type reagent kit 7 rotates at a high speed following the rotating drum 3, when the waste liquid enters the centrifugal chamber 11 from the through hole 33, it will be subjected to a large centrifugal force. Therefore, it will impact the side wall of the centrifugal chamber 11, so that the waste liquid may splash everywhere and may contaminate the centrifugal column type reagent kit 7. Therefore, for this problem,

[0041] Based on Embodiment 1, referring to the attached drawings of the specification Figure 9 、 10 、11, the centrifuge further includes a protective cover 4. The protective cover 4 includes an annular top plate 41 and an annular side plate 42 vertically installed on the outer edge of the top plate 41. A sleeve hole 411 for exposing the top surface of the rotating drum 3 is opened in the middle of the top plate 41. The bottom end of the side plate 42 is a snap ring 43 with a circular cross section. The diameter of the circular cross section of the snap ring 43 is greater than the thickness of the side plate 42, and the outer diameter of the snap ring 43 is greater than or equal to the inner diameter of the centrifugal chamber 11. The protective cover 4 is fixedly connected to the side wall of the centrifugal chamber 11 through the snap ring 43 at the bottom end of the side plate 42. Two handles 44 are symmetrically arranged on the top surface of the top plate 41. The handles 44 facilitate the installation of the protective cover 4 in the centrifugal chamber 11 and the removal of the protective cover 4 from the centrifugal chamber 11.

[0042] Preferably, an annular inner plate is also vertically installed on the inner edge of the top plate 41. The height of the inner plate is less than that of the side plate 42. The inner plate is parallel to the side of the drum 3, and the bottom is close to the outer wall of the drum 3, so that the top plate 41, the side plate 42 and the inner plate enclose a shielding area for the waste liquid, and the waste liquid entering the centrifugal chamber 11 from the through hole 33 is restricted within the above-mentioned shielding area, so as to prevent the waste liquid from splashing onto the top surface of the drum 3 during the high-speed rotation of the drum 3 and polluting the centrifugal column kit 7.

[0043] Example 3

[0044] Since the adsorption column currently in use is the same as the adsorption column 71 in Example 1, the difference between the currently used collection tube and the collection tube 72 in Example 1 is only that an opening 722 is provided at the bottom of the collection tube 72 in Example 1. After the currently used adsorption column is sleeved on the collection tube, in order to ensure that the waste liquid generated in the adsorption column can completely enter the collection tube, a certain space (the volume of this space is greater than the volume of the adsorption column) needs to be reserved between the bottom of the adsorption column and the bottom of the collection tube to collect the waste liquid. At the same time, referring to the attached Figure 1 of the specification, it can be seen that in step 5, it is recorded that the volume of the commonly used adsorption column (that is, the adsorption column 71 in Example 1) is 700 μL, and the volume of the mixed liquid obtained after steps 1-4 is much larger than 700 μL. Therefore, the mixed liquid can only be added to the adsorption column in portions for centrifugation, which will increase the number of operations and the working intensity of the operator;

[0045] Therefore, in view of the above problems, based on the technical solutions in Example 1 or 2, an opening 722 is provided at the bottom of the collection tube 72. Then, the waste liquid centrifuged from the adsorption column 71 into the collection tube 72 will not be collected in the collection tube 72, but will be centrifuged away from the opening 722. Then, the inventor provides the following solutions.

[0046] Referring to the attached Figure 12 and 13 of the specification, extend the length of the part of the extension column 711 inserted into the collection tube 72 to compress the space between the bottom of the adsorption column 71 and the bottom of the collection tube 72, so as to increase the volume inside the adsorption column 71, and / or extend the length of the part outside the collection tube 72 to increase the total volume of the inner cavity of the column 711, so that more mixed liquid can be contained for subsequent nucleic acid extraction operations, which can reduce the number of operations, improve work efficiency, and reduce the working intensity of the operator.

[0047] The usage method of the nucleic acid extraction device by the centrifugal column method in this patent is as follows: fixedly sleeve the rotating shaft 12 with the shaft sleeve 31, the rotating shaft pin is matched and clamped in the card slot 34, use the fixing screw to fixedly install the drum 3 on the rotating shaft 12, and cover the protective cover 4 in the centrifugal chamber 11 through the handle 44, and the top surface of the drum 3 exposes from the top surface of the sleeve hole 411;

[0048] According to the attachment Figure 1 The mixed liquid is obtained by following steps 1-4 in the attachment. The adsorption column 71 is sleeved into the collection tube 72, and the mixed liquid is transferred into the adsorption column 71. The collection tube 72 is inserted into the mounting hole 32, and the machine cover 2 is covered. According to step 5, the centrifuge is started and centrifuged at 12000 rpm (~13400 xg) for 30 seconds. During centrifugation, the waste liquid generated in the adsorption column 71 first enters the space between the bottom of the adsorption column 71 and the bottom of the collection tube 72, and then is quickly centrifuged away from the opening 722. The subsequent waste liquid enters the centrifugation chamber 11 through the through hole 33 and is in a waste liquid shielding area surrounded by the top plate 41 and the side plate 42. Then the waste liquid is drained to the waste liquid bottle 6 through the liquid leakage hole 13 and the waste liquid pipe 5. At the same time, when performing subsequent steps 6-9, the waste liquid generated in the adsorption column 71 will also pass through the opening 722, the mounting hole 32, the through hole 33, the centrifugation chamber 11, the liquid leakage hole 13 and the waste liquid pipe 5 in sequence, and finally be drained to the waste liquid bottle 6.

[0049] Obviously, it is possible to modify and / or add components to the above-mentioned nucleic acid extraction device by centrifugal column method and the corresponding method without departing from the scope and field of the present utility model.

[0050] It is also clear that although the present utility model has described the nucleic acid extraction device by centrifugal column method in detail, those skilled in the art will surely be able to obtain many other equivalent forms of nucleic acid extraction devices by centrifugal column method and the corresponding methods. The nucleic acid extraction device by centrifugal column method and the corresponding methods have the features as described in the claims, and thus are all within the scope of the protection defined thereby.

Claims

1. A centrifugal column method nucleic acid extraction device, characterized in that: The invention comprises a centrifuge and a centrifugal column reagent kit (7); the centrifuge comprises a body (1), a cover (2) and a drum (3); a centrifugal chamber (11) is arranged in the middle of the top surface of the body (1); the drum (3) is installed in the centrifugal chamber (11); a motor for indirectly or directly driving the drum (3) to rotate is arranged in the body (1); the centrifugal column reagent kit (7) comprises an adsorption column (71) and a collection tube (72); the adsorption column (71) is detachably inserted into the collection tube (72) through the top opening of the collection tube (72); the drum (3) is in the shape of a disk as a whole; a plurality of mounting holes (32) for oblique insertion of the collection tube (72) are arranged in a circular array on the top surface of the drum (3); An opening (722) is provided at the bottom of the collecting tube (72); a through hole (33) extending from the side or bottom of the drum (3) is provided at the bottom of the mounting hole (32); a liquid leakage hole (13) is provided at the bottom surface of the centrifugal chamber (11); a waste liquid pipe (5) is connected to the bottom of the liquid leakage hole (13); the other end of the waste liquid pipe (5) extends out of the body (1) and is connected to a waste liquid bottle (6); the opening (722), the mounting hole (32), the through hole (33), the centrifugal chamber (11), the liquid leakage hole (13) and the waste liquid pipe (5) together constitute a drainage channel for the waste liquid, so that the waste liquid generated by the adsorption column (71) is drained into the waste liquid bottle (6) through the drainage channel.

2. The nucleic acid extraction device according to claim 1, characterized in that: The centrifuge further comprises a protective cover (4), the protective cover (4) comprising a circular top plate (41) and a side plate (42) vertically mounted on the outer edge of the top plate (41), a sleeve hole (411) for exposing the top surface of the drum (3) is opened in the middle of the top plate (41), the protective cover (4) is fixedly connected to the side wall of the centrifugal chamber (11) through the side plate (42), and at least one handle (44) is arranged on the top surface of the top plate (41).

3. The nucleic acid extraction device according to claim 2, characterized in that: The bottom end of the side plate (42) is a snap ring (43) with a circular cross section, the diameter of the circular cross section of the snap ring (43) is greater than the thickness of the side plate (42), and the outer diameter of the snap ring (43) is greater than or equal to the inner diameter of the centrifugal chamber (11), and the protective cover (4) is fixedly connected to the side wall of the centrifugal chamber (11) by the snap ring (43) at the bottom end of the side plate (42).

4. The nucleic acid extraction device according to claim 1, characterized in that: A rotating shaft (12) is arranged in the middle of the centrifugal chamber (11), and the rotating shaft (12) is driven to rotate indirectly or directly by a motor arranged in the body (1). A rotating shaft pin is fixed and vertically arranged on the rotating shaft (12). A hollow shaft sleeve (31) is arranged in the middle of the rotating drum (3), and a clamping groove (34) is opened on the bottom surface of the shaft sleeve (31). The rotating shaft (12) and the shaft sleeve (31) are fixedly sleeved, and the rotating shaft pin is matched and clamped in the clamping groove (34).

5. The nucleic acid extraction device according to claim 1, characterized in that: The waste liquid pipe (5) is an inclined pipe, the end of which is connected to the leakage hole (13) is the high end of the inclined pipe, and the end of which is connected to the waste liquid bottle (6) is the low end of the inclined pipe.

6. The nucleic acid extraction device according to claim 1, characterized in that: The adsorption column (71) comprises a hollow column (711) and an end cover (712) arranged on the top of the column (711); the end cover (712) is used to close the top opening of the column (711); the bottom end of the column (711) is a conical structure; a convex column is arranged on the inner side of the conical structure; an adsorption film (714) is arranged on the convex column; the adsorption film (714) is fixed on the convex column by a fixing ring (715); and a plurality of clamping strips (713) arranged in a vertical direction are arranged in a circular array on the upper outer side of the column (711); A clamping ring (721) is horizontally arranged on the inner side of the top opening of the collecting tube (72); the adsorption column (71) is detachably inserted into the collecting tube (72) through the top opening of the collecting tube (72), and is clamped and fixed by a clamping strip (713) and the clamping ring (721).

7. The nucleic acid extraction device according to claim 6, characterized in that: The length of the portion of the column (711) that is inserted into the collecting tube (72) is extended, and / or the length of the portion outside the collecting tube (72) is extended to increase the total volume of the inner cavity of the column (711).

Images