Filtering device for plasmid extraction

Abstract

The utility model relates to the technical field of plasmid extraction, in particular to a filtering device for plasmid extraction, which comprises a supporting assembly, a bearing assembly and a pressurizing assembly, the supporting assembly comprises a base, a supporting column and a top plate, and the upper end and the lower end of the supporting column are respectively connected with the center of the top plate and the center of the base. The bearing assembly comprises a waste liquid collecting pipe, a stock solution pipe and a filtering gasket, a plurality of groups of collecting grooves are formed in the base, the waste liquid collecting pipe is matched with the collecting grooves, the base provides stable support for the waste liquid collecting pipe, a supporting column for supporting the top plate is arranged on the base, and the top plate is arranged on the supporting column. A waste liquid collecting pipe and a stock solution pipe are placed at designated positions, the stock solution pipe is placed on a top plate through a partition plate, a pressurizing cover is in threaded connection with the stock solution pipe, a piston is driven through a pressing rod, the interior of the stock solution pipe can be pressurized, and liquid enters the waste liquid collecting pipe through a filtering gasket.

Description

Technical Field

[0001] The utility model relates to the technical field of plasmid extraction, in particular to a filtering device for plasmid extraction. Background Technique

[0002] A plasmid is a circular DNA molecule that exists outside the bacterial chromosome and can replicate independently and be stably inherited. The common method for its extraction and purification is the column extraction method, which requires the use of a filter column to filter the bacterial solution in order to obtain the target plasmid in the bacteria.

[0003] The application number is CN202122210707.3, and the patent name is a filtering device for plasmid extraction, including a support plate, a top plate and a filter column. The filter column is inserted through the support plate, and a filter element is arranged at the inner bottom of the filter column. The top plate is located above the support plate. An electric push rod with an output end connected to the bottom of the top plate is installed on the support plate. An air pipe is arranged at the bottom of the top plate, and the bottom of the air pipe extends to the bottom of the bacterial solution inside the filter column. An air pump with an exhaust end connected to the top end of the air pipe is installed on the top of the top plate. A control panel electrically connected to the electric push rod and the air pump is installed on the top plate. By setting the support plate, the top plate, the electric push rod, the air pipe, the air pump and the control panel, when filtering the bacterial solution, gas is injected into the bottom of the bacterial solution in the filter column by using the air pump and the air pipe. The gas can push the impurities on the filter element at the inner bottom of the filter column, reduce the impurities deposited on the filter element, ensure the filtering speed of the filter element, and thus improve the filtering efficiency of the device.

[0004] The above structure also has the following disadvantages: 1. The structure is complex. The control panel is used in conjunction with the electric push rod and the air pump, and the structure is complex. When a local part is damaged, it is not convenient to carry out maintenance and inspection. 2. The device needs to be plugged in at a specific position for use, and the usage limitation is relatively high. Summary of the Utility Model

[0005] (1) Technical Problem to be Solved

[0006] In view of the deficiencies of the prior art, the utility model provides a filtering device for plasmid extraction.

[0007] (2) Technical Solution

[0008] To achieve the above object, the present utility model provides the following technical solutions: A filtering device for plasmid extraction, comprising a support assembly, a carrying assembly, and a pressurizing assembly. The support assembly includes a base, a support column, and a top plate. The upper and lower ends of the support column are respectively connected to the center of the top plate and the base. The carrying assembly includes a waste liquid collection tube, a stock solution tube, and a filter gasket. A plurality of groups of collection grooves are provided on the base, and the waste liquid collection tube is adapted to the collection grooves. The waste liquid collection tube is provided with an upper opening. A plurality of groups of carrying holes adapted to the collection grooves are provided on the top plate. A partition is provided outside the stock solution tube, and a filter gasket is provided inside the stock solution tube. The upper part of the stock solution tube is cylindrical, and the lower part of the stock solution tube passes through the top plate and is in an inverted conical shape. The partition is provided above the top plate, and the output end below the stock solution tube is adapted to the input end of the waste liquid collection tube. The pressurizing assembly includes a pressurizing cover, a piston, and a pressure rod. The piston is provided inside the pressurizing cover, the pressure rod penetrates through the top wall of the pressurizing cover and is connected to the piston, and the inner wall of the pressurizing cover is threadedly connected to the outer wall of the stock solution tube.

[0009] To facilitate closing the stock solution tube, the present utility model is improved in that an auxiliary tube is further provided at the lower opening of the stock solution tube, and a plug is provided below the auxiliary tube.

[0010] To facilitate driving the pressure rod, the present utility model is improved in that a pressure piece is provided above the pressure rod.

[0011] Preferably, auxiliary plates are further provided on the left and right sides above the pressurizing cover.

[0012] To facilitate lifting the waste liquid collection tube, the present utility model is improved in that a flange is provided at the upper edge of the waste liquid collection tube.

[0013] Preferably, friction lines are provided on the outside of the partition.

[0014] (III) Beneficial effects

[0015] Compared with the prior art, the present utility model provides a filtering device for plasmid extraction, which has the following beneficial effects:

[0016] For the filtering device for plasmid extraction, the base provides stable support for the waste liquid collection tube. A support column for supporting the top plate is provided on the base. The waste liquid collection tube and the stock solution tube are placed at designated positions. The stock solution tube is placed on the top plate through the partition. The pressurizing cover is threadedly connected to the stock solution tube. By driving the piston with the pressure rod, the inside of the stock solution tube can be pressurized, so that the liquid passes through the filter gasket and enters the waste liquid collection tube. Description of the drawings

[0017] Figure 1 It is a schematic bottom view of the structure of the present utility model;

[0018] Figure 2 This is the front view schematic diagram of the partial explosion of the structure of the present utility model;

[0019] Figure 3 This is the bottom view schematic diagram of the partial explosion of the structure of the present utility model;

[0020] Figure 4 This is the front view schematic diagram of the structure of the present utility model.

[0021] In the figure: 1, base; 2, support column; 3, top plate; 4, waste liquid collection pipe; 5, stock solution pipe; 6, filter gasket; 7, partition board; 8, pressure hood; 9, piston; 10, pressure rod; 11, auxiliary pipe; 12, plug; 13, pressing piece; 14, auxiliary plate; 15, flange. Specific embodiments

[0022] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0023] Please refer to Figures 1-4 , a filtering device for plasmid extraction, including a support assembly, a bearing assembly and a pressurizing assembly. The support assembly includes a base 1, a support column 2 and a top plate 3. The upper and lower ends of the support column 2 are respectively connected to the centers of the top plate 3 and the base 1. The bearing assembly includes a waste liquid collection pipe 4, a stock solution pipe 5 and a filter gasket 6. A plurality of groups of collection grooves are provided on the base 1, and the waste liquid collection pipe 4 is adapted to the collection grooves. The waste liquid collection pipe 4 is provided with an upper opening. A plurality of groups of bearing holes adapted to the collection grooves are provided on the top plate 3. A partition board 7 is provided outside the stock solution pipe 5, and a filter gasket 6 is provided inside the stock solution pipe 5. The upper part of the stock solution pipe 5 is cylindrical, and the lower part of the stock solution pipe 5 passes through the top plate 3 and is in an inverted cone shape. The partition board 7 is provided above the top plate 3, and the output end below the stock solution pipe 5 is adapted to the input end of the waste liquid collection pipe 4. The pressurizing assembly includes a pressure hood 8, a piston 9 and a pressure rod 10. The piston 9 is provided inside the pressure hood 8, the pressure rod 10 penetrates through the top wall of the pressure hood 8 and is connected to the piston 9, and the inner wall of the pressure hood 8 is threadedly connected to the outer wall of the stock solution pipe 5.

[0024] During actual use, support columns 2 are provided on the base 1 to provide stable support for the top plate 3. Each waste liquid collection pipe 4 is inserted into the corresponding collection groove of the base 1, and then the stock solution pipe 5 is placed on the top plate 3. The partition 7 on the stock solution pipe 5 ensures its stable fixation on the top plate 3. Stock solution is placed in the stock solution pipe 5, and the liquid flows through the filter gasket 6 into the waste liquid collection pipe 4. The pressure cover 8 is threadedly connected to the stock solution pipe 5. Initially, the piston 9 and the pressure rod 10 are in a relatively high position. After the pressure cover 8 is fully connected to the stock solution pipe 5, by pressing down the pressure rod 10, the piston 9 moves downward, and the air pressure in the stock solution pipe 5 increases, causing the stock solution to pass through the filter gasket 6.

[0025] During actual use, an auxiliary pipe 11 is also provided at the lower opening of the stock solution pipe 5, and a plug 12 is provided below the auxiliary pipe 11. Initially, the plug 12 closes the auxiliary pipe 11. When the stock solution pipe 5 and the pressurizing assembly are not assembled, the plug 12 is opened, and the stock solution can pass through the filter gasket 6. When the stock solution pipe 5 and the pressurizing assembly are assembled, the plug 12 is opened, and by pressing down the pressure rod 10, the stock solution passes through the filter gasket 6.

[0026] During actual use, a pressure plate 13 is provided above the pressure rod 10, and auxiliary plates 14 are also provided on the left and right sides above the pressure cover 8. The pressure plate reduces the pressure of directly pressing down the pressure rod 10, and the auxiliary plates 14 facilitate the stable driving of the pressure plate by hand.

[0027] In this embodiment, a flange 15 is provided at the upper edge of the waste liquid collection pipe 4, which facilitates the taking of the waste liquid collection pipe 4.

[0028] During actual use, friction lines are provided on the outside of the partition 7, which facilitates unlocking the bearing assembly and the pressurizing assembly.

[0029] To illustrate in detail the possible application scenarios, technical principles, specific implementable solutions, achievable purposes and effects of this application, the following is described in detail with reference to the specific examples listed and in conjunction with the drawings. The embodiments described herein are only used to more clearly illustrate the technical solutions of this application, so they are only examples and cannot be used to limit the protection scope of this application.

[0030] As used herein, the term "embodiment" means that the specific features, structures, or characteristics described in connection with the embodiment may be included in at least one embodiment of this application. The term "embodiment" that appears in various positions in the specification does not necessarily refer to the same embodiment, nor does it particularly limit its independence or relevance to other embodiments. In principle, in this application, as long as there are no technical contradictions or conflicts, the technical features mentioned in each embodiment can be combined in any way to form corresponding implementable technical solutions.

[0031] Unless otherwise defined, the meanings of technical terms used herein are the same as those commonly understood by those skilled in the technical field to which this application belongs; the use of relevant terms herein is only for describing specific embodiments and is not intended to limit this application.

[0032] Although embodiments of the present utility model have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present utility model, and the scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. A filtering device for plasmid extraction, comprising a supporting component, a bearing component and a pressurizing component, characterized in that: The support assembly comprises a base (1), a support column (2) and a top plate (3); the upper and lower ends of the support column (2) are respectively connected to the top plate (3) and the center of the base (1); the bearing assembly comprises a waste liquid collection pipe (4), a raw liquid pipe (5) and a filter gasket (6); the base (1) is provided with a plurality of groups of collecting grooves; the waste liquid collection pipe (4) is adapted to the collection grooves; the waste liquid collection pipe (4) is provided with an upper opening; the top plate (3) is provided with a plurality of groups of bearing holes adapted to the collection grooves; a partition plate (7) is provided on the outer side of the raw liquid pipe (5); a filter gasket (6) is provided inside the raw liquid pipe (5); A filter gasket (6) is provided, the upper part of the raw liquid tube (5) is cylindrical, the lower part of the raw liquid tube (5) passes through the top plate (3) and is in an inverted cone shape, the partition plate (7) is arranged above the top plate (3), the lower output end of the raw liquid tube (5) is adapted to the input end of the waste liquid collection tube (4), the pressurizing component comprises a pressurizing cover (8), a piston (9) and a pressure rod (10), the piston (9) is arranged in the pressurizing cover (8), the pressure rod (10) passes through the top wall of the pressurizing cover (8) and is connected to the piston (9), and the inner wall of the pressurizing cover (8) is connected to the outer wall of the raw liquid tube (5) by threads.

2. A filtering device for plasmid extraction according to claim 1, characterized in that: An auxiliary tube (11) is also provided at the lower opening of the raw liquid tube (5), and a plug (12) is provided below the auxiliary tube (11).

3. A filtering device for plasmid extraction according to claim 2, characterized in that: A pressing sheet (13) is arranged above the pressing rod (10).

4. A filtering device for plasmid extraction according to claim 3, characterized in that: Auxiliary plates (14) are also provided on the left and right sides above the pressure cover (8).

5. A filtering device for plasmid extraction according to claim 4, characterized in that: The upper edge of the waste liquid collection pipe (4) is provided with a flange (15).

6. A filtering device for plasmid extraction according to claim 5, characterized in that: The outer side of the partition (7) is provided with friction patterns.

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