Sample fixing device for ultrasonic cell disruptor

Abstract

The utility model discloses a sample fixing device for an ultrasonic cell disruptor, which relates to the technical field of biology and comprises an ice-water bath container with a cylindrical structure with a hollow top; the sample fixing frame comprises a sample fixing buckle composed of a plurality of compression springs and four internally-hollow arc-shaped buckles, the four sample fixing buckles are connected in a penetrating mode through an elastic ring to form a cylinder, and the compression springs are arranged between the inner wall of the ice-water bath container and the outer wall of the sample fixing buckle; according to the technical scheme provided by the utility model, through the arrangement of a combined structure of the compression spring and the elastic ring, the sample fixing frame can be kept stable in the ice-water bath container, and the sample tube is ensured not to displace or vibrate in the ultrasonic treatment process. The position of the centrifugal tube is effectively fixed, and a sample can be continuously placed in an ice-water bath, so that denaturation and inactivation of protein are effectively prevented.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, and particularly relates to a sample fixing device for an ultrasonic cell crusher. Background Art

[0002] During the traditional ultrasonic cell crushing process, the sample tube is usually directly placed in an ice-water bath or fixed using a simple clamp. For example, patent application 202121775876.5 discloses a sampling device for an ultrasonic cell crusher, including a crushing tank, wherein a rotating rod is arranged inside the crushing tank; a separating rod arranged on the outer wall of the rotating rod; an ultrasonic generator arranged below the rotating rod; a first gravity sensor arranged at the bottom of the crushing tank; a controller arranged on one side of the first gravity sensor; a support plate installed below the first gravity sensor; a cover plate installed on the top of the crushing tank; a display arranged on one side of the crushing tank; and a shunt tank arranged on the other side of the crushing tank. By arranging the shunt tank, the stratified liquid can be effectively received, thereby achieving the purpose of stratified sampling.

[0003] However, at present, the sample tube is prone to displacement or vibration during the ultrasonic treatment process, which affects the crushing effect and the consistency of the sample, and cannot effectively fix the position of the centrifuge tube, resulting in the temperature of the ice-water mixture in the ice-water bath being difficult to maintain constant, causing the sample temperature to fluctuate, and affecting the activity and integrity of the cells. Summary of the Utility Model

[0004] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide a sample fixing device for an ultrasonic cell crusher. Through the combined structure of a compression spring and an elastic ring, the sample can be kept stable in the ice-water bath container, ensuring that the sample tube does not displace or vibrate during the ultrasonic treatment process, enabling the sample to be continuously placed in the ice-water bath, and effectively preventing the denaturation and inactivation of proteins.

[0005] In view of this, the utility model provides the following technical solutions.

[0006] A sample fixing device for an ultrasonic cell crusher, comprising:

[0007] An ice-water bath container, which is a cylindrical structure with a hollow top;

[0008] A sample fixing rack, the sample fixing rack includes a sample fixing buckle composed of a plurality of compression springs and four internally hollow arc-shaped buckles, and the four sample fixing buckles are connected through an elastic ring to form a cylinder, and the compression springs are arranged between the inner wall of the ice-water bath container and the outer wall of the sample fixing buckle.

[0009] Optionally, a first spring fixing buckle is fixedly connected to the inner wall of the ice-water bath container, a second spring fixing buckle is fixedly connected to the outer wall of the sample fixing buckle, and the compression springs are respectively installed on the first spring fixing buckle and the second spring fixing buckle.

[0010] Optionally, the material of the ice-water bath container is stainless steel.

[0011] Optionally, the material of the compression spring is carbon spring steel or stainless steel.

[0012] Optionally, an anti-slip material layer is provided on the inner side of the arc-shaped buckle of the sample fixing buckle.

[0013] Optionally, a heat-insulating layer is provided at the bottom of the ice-water bath container.

[0014] As can be seen from the above technical solutions, the embodiments of the present utility model have the following advantages:

[0015] 1. The sample fixing device for an ultrasonic cell disrupter of the present utility model, through the combined structure setting of the compression spring and the elastic ring, enables the sample fixing rack to remain stable in the ice-water bath container, ensuring that the sample tube will not be displaced or vibrated during the ultrasonic treatment process. It effectively fixes the position of the centrifuge tube, and the sample can be continuously placed in the ice-water bath, effectively preventing the denaturation and inactivation of proteins.

[0016] 2. The sample fixing device for an ultrasonic cell disrupter of the present utility model, the heat-insulating layer at the bottom of the ice-water bath container reduces the temperature loss of the ice-water mixture, helps to maintain a stable low-temperature environment, ensures the temperature consistency during the sample treatment process, and the anti-slip material layer added to the inner side of the sample fixing buckle increases the friction force, effectively preventing the sample tube from loosening during the treatment process and improving the reliability of the overall system.

[0017] These features and advantages of the present utility model will be disclosed in detail in the following specific embodiments and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The following further describes the present utility model with reference to the drawings:

[0019] Figure 1 is a schematic structural diagram of the present utility model;

[0020] Figure 2 is a schematic top view structural diagram of the present utility model;

[0021] Figure 3 is the present utility model Figure 2 The enlarged view of the structure at A in.

[0022] Description of the reference numerals: 1. Ice-water bath container; 11. First spring fixing buckle; 2. Sample fixing buckle; 21. Second spring fixing buckle; 3. Compression spring; 4. Elastic ring; 5. Anti-slip material layer; 6. Thermal insulation layer. Detailed implementation manners

[0023] The technical solutions of the embodiments of the present utility model will be explained and described below with reference to the accompanying drawings of the embodiments of the present utility model. However, the following embodiments are only the preferred embodiments of the present utility model and not all of them. Based on the embodiments in the implementation manners, other embodiments obtained by those skilled in the art without creative efforts all fall within the protection scope of the present utility model.

[0024] The following specifically describes an embodiment of a sample fixing device for an ultrasonic cell crusher according to an embodiment of the present utility model with reference to the accompanying drawings.

[0025] Embodiment

[0026] For ease of understanding, please refer to Figures 1 to 3 , an embodiment of a sample fixing device for an ultrasonic cell crusher provided by the present utility model, comprising:

[0027] An ice-water bath container 1, the ice-water bath container 1 is a cylindrical structure with a hollow top, and the material can be selected from stainless steel or plastic, and is used for containing an ice-water mixture;

[0028] A sample fixing rack, the sample fixing rack includes a sample fixing buckle 2 composed of a plurality of compression springs 3 and four arc-shaped buckles with hollow interiors, and the four sample fixing buckles are connected through an elastic ring 4 to form a cylinder, and the compression springs 3 are arranged between the inner wall of the ice-water bath container 1 and the outer wall of the sample fixing buckle 2.

[0029] It should be noted that the ice-water bath container 1 is a cylindrical structure with an open top and is made of stainless steel and is used for containing an ice-water mixture. The size of the ice-water bath container 1 can be adjusted according to actual needs. However, generally, the diameter should be large enough to accommodate the sample fixing rack and leave a certain space for placing the ice-water mixture. The sample fixing rack includes a sample fixing buckle 2 composed of four arc-shaped buckles with hollow interiors, and these sample fixing buckles are connected together through an elastic ring 4 to form a telescopic cylindrical structure. The compression spring 3 is located between the first spring fixing buckle 11 on the inner wall of the ice-water bath container 1 and the second spring fixing buckle 21 on the sample fixing buckle 2. The elastic ring 4 is used to connect the four sample fixing buckles to provide appropriate elasticity to ensure that the sample fixing buckle can firmly hold the sample tube. The material of the compression spring can be carbon spring steel or stainless steel.

[0030] In some embodiments, a first spring fastener 11 is fixedly connected to the inner wall of the ice-water bath container 1, a second spring fastener 21 is fixedly connected to the outer wall of the sample fastener 2, and the compression springs 3 are respectively installed on the first spring fastener 11 and the second spring fastener 21.

[0031] It should be noted that the compression springs 3 are respectively installed on the first spring fastener 11 and the second spring fastener 21 to fix the position between the sample fastener 2 and the ice-water bath container 1.

[0032] In some embodiments, the ice-water bath container 1 is made of stainless steel.

[0033] In some embodiments, the compression springs 3 are made of carbon spring steel or stainless steel.

[0034] In some embodiments, an anti-slip material layer 5 is provided on the inner side of the arc-shaped buckle of the sample fastener 2. It should be noted that an anti-slip material layer 5, such as rubber or other materials with a high coefficient of friction, is added to the inner side of the arc-shaped buckle of the sample fastener 2 to increase the friction force on the sample tube and ensure that the sample tube does not loosen during the ultrasonic treatment process.

[0035] In some embodiments, a heat insulation layer 6 is provided at the bottom of the ice-water bath container 1 to reduce the temperature loss of the ice-water mixture, improve the cooling effect and extend the service time of the ice-water mixture.

[0036] It should be noted that a heat insulation layer 6, such as polyurethane foam or other materials with good heat insulation performance, is added to the bottom of the ice-water bath container 1 to reduce the temperature loss of the ice-water mixture, improve the cooling effect, and extend the service time of the ice-water mixture.

[0037] Working principle: Prepare the ice-water mixture: First, add an appropriate amount of ice-water mixture to the ice-water bath container 1.

[0038] Assemble the sample fixing rack: Place the sample fixing rack into the ice-water bath container 1, and ensure an appropriate distance between the sample fastener 2 and the inner wall of the container through the compression springs 3.

[0039] Fix the sample tube: Place the sample tube into the sample fastener 2, and use the elasticity of the sample fastener and the anti-slip material layer to ensure the stability of the sample tube.

[0040] Perform ultrasonic treatment: Place the entire sample fixing device into an ultrasonic cell disruptor and perform cell disruption operations according to predetermined parameters.

[0041] As described above, the above embodiments are only used to illustrate the technical solutions of the present application, rather than limiting it; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the various embodiments of the present application.

Claims

1. A sample fixing device for an ultrasonic cell pulverizer, characterized in that: include: An ice-water bath container (1), wherein the ice-water bath container (1) is a cylindrical structure with a hollow top; A sample fixing rack comprises a plurality of compression springs (3) and a sample fixing buckle (2) composed of four internally hollow arc buckles, wherein the four sample fixing buckles are connected through elastic rings (4) to form a cylinder, and the compression spring (3) is arranged between the inner wall of an ice-water bath container (1) and the outer wall of the sample fixing buckle (2).

2. A sample fixing device for an ultrasonic cell pulverizer according to claim 1, characterized in that: The inner wall of the ice-water bath container (1) is fixedly connected to a first spring fixing buckle (11), the outer wall of the sample fixing buckle (2) is fixedly connected to a second spring fixing buckle (21), and the compression spring (3) is respectively mounted on the first spring fixing buckle (11) and the second spring fixing buckle (21).

3. A sample fixing device for an ultrasonic cell pulverizer according to claim 1, characterized in that: The material of the ice water bath container (1) is stainless steel.

4. A sample fixing device for an ultrasonic cell pulverizer according to claim 1, characterized in that: The material of the compression spring (3) is carbon spring steel or stainless steel.

5. A sample fixing device for an ultrasonic cell pulverizer according to claim 1, characterized in that: An anti-slip material layer (5) is provided on the inner side of the arc-shaped buckle of the sample fixing buckle (2).

6. A sample fixing device for an ultrasonic cell pulverizer according to claim 1, characterized in that: The bottom of the ice-water bath container (1) is provided with a heat-insulating layer (6).

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