Cell fluid infusion device

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell fluid infusion device which comprises a working table, a mounting table is fixed on the top surface of the working table, and a heat preservation device is fixed at the top of the mounting table. According to the utility model, the loss of fluid infusion temperature can be reduced, the stability of cell culture is facilitated, and the accuracy of experiments, especially the culture experiments of temperature-sensitive cells, is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell fluid replenishing device. Background Art

[0002] Cell culture technology is usually used in scientific, pharmaceutical and biomedical research applications. In cell culture, cells are usually placed in a culture medium containing nutrients, growth factors and an appropriate pH value, while providing appropriate temperature and humidity conditions to promote the normal growth and proliferation of cells. Existing cell culture technologies improve the work efficiency of experimental personnel by setting up an automatic fluid replenishing device. However, before using the fluid replenishment, it is necessary to rinse the cells with preheated normal saline or buffer solution to remove cell metabolites and residual culture medium, and put the newly prepared fluid replenishment into a thermostat for preheating and temperature control before pouring it into the rinsed cell culture dish or cell culture bag. And in order to avoid the generation of bubbles and reduce the mechanical damage to cells, the experimental personnel need to pour the fluid replenishment gently and slowly. During this process, heat loss will inevitably occur, resulting in a change in the temperature of the fluid replenishment, affecting the cell culture experiment, especially the growth of temperature-sensitive cells and the accuracy of the experiment. Summary of the Utility Model

[0003] In order to solve the problems in the above background art, the utility model provides a cell fluid replenishing device, which can reduce the heat loss of the fluid replenishment, is beneficial to the stability of cell culture, and ensures the accuracy of the experiment, especially for the cell culture experiment of temperature-sensitive cells.

[0004] The solution adopted by the utility model to solve its technical problems is: a cell fluid replenishing device, including a workbench, an installation table is fixedly arranged on the top surface of the workbench, and a heat preservation device is fixedly arranged on the top of the installation table.

[0005] By adopting the above technical solution, the heat preservation device can provide heat during fluid replenishment, reduce the heat loss of the fluid replenishment, and improve the accuracy of the experiment.

[0006] Furthermore, the heat preservation device includes a heat preservation box for containing heat preservation liquid, a fluid replenishing cylinder for injecting fluid replenishment, and a stirring member for stirring; the heat preservation box is fixedly connected to the top surface of the installation table, the fluid replenishing cylinder is detachably installed in the heat preservation box, and the stirring member is arranged in the fluid replenishing cylinder.

[0007] By adopting the above technical solution, it is convenient for experimental personnel to replenish fluid, and heat and stir before fluid replenishment to make the components in the fluid replenishment evenly dispersed.

[0008] Furthermore, a heat preservation cover is arranged on the top of the heat preservation box, and the heat preservation cover is provided with a positioning hole for fixing the fluid replenishing cylinder.

[0009] By adopting the above technical solutions, it is convenient for the experimenter to fix the liquid replenishing cylinder.

[0010] Furthermore, a clamping ring is provided at the top of the liquid replenishing cylinder, and the clamping ring is clamped in the positioning hole.

[0011] By adopting the above technical solutions, the installation and disassembly of the liquid replenishing cylinder are facilitated.

[0012] Furthermore, a temperature display is provided at the bottom of the incubator.

[0013] By adopting the above technical solutions, the experimenter can observe the temperature change inside the incubator, which is convenient for the experimenter to adjust the temperature setting.

[0014] Furthermore, a liquid replenishing pipe is provided at the bottom of the liquid replenishing cylinder. One end of the liquid replenishing pipe communicates with the bottom of the liquid replenishing cylinder, and the other end passes through the side wall of the incubator and communicates with the outside.

[0015] By adopting the above technical solutions, the liquid replenishing pipe can transfer the liquid in the liquid replenishing cylinder to the culture bag.

[0016] Furthermore, a filter screen is provided at the end of the liquid replenishing pipe that communicates with the liquid replenishing cylinder.

[0017] By adopting the above technical solutions, the filter screen can filter impurities in the liquid replenishing solution and also prevent the stirring member from flowing into the liquid replenishing pipe and blocking the liquid replenishing pipe.

[0018] Furthermore, a heat preservation sleeve is provided outside the liquid replenishing pipe.

[0019] By adopting the above technical solutions, the loss of temperature inside the liquid replenishing pipe is prevented.

[0020] Furthermore, the workbench is provided with a folding support frame for support

[0021] By adopting the above technical solutions, the folding support frame can support the cell culture belt, which is convenient for the experimenter to replenish the liquid.

[0022] In summary, the beneficial effects of the present utility model are as follows: when the cells need to replace the liquid replenishing solution, the heat preservation device can preheat the cell liquid replenishing solution and keep it under constant temperature control; a stirring member is provided inside the liquid replenishing cylinder, which can stir the liquid replenishing solution before use to ensure that various components in the liquid replenishing solution are evenly mixed; a temperature display is provided at the bottom of the incubator, and the experimenter can observe the temperature inside the incubator, which is convenient for the experimenter to set and adjust the temperature; a folding support frame is provided on the workbench, which is convenient for the experimenter to fix the cell culture bag; a heat preservation sleeve is provided outside the liquid replenishing pipe to prevent the temperature loss of the liquid replenishing solution during infusion; a filter screen is provided at the bottom of the liquid replenishing cylinder, which can not only filter impurities but also prevent the stirring member from entering the liquid replenishing pipe.

[0023] The above description is only an overview of the technical solution of the present utility model. In order to be able to understand the technical means of the present utility model more clearly, it can be implemented according to the content of the description. And in order to make the above and other purposes, features and advantages of the present utility model more obvious and understandable, the following preferred embodiments are specifically given and described in detail in conjunction with the accompanying drawings as follows. Description of the Drawings

[0024] Figure 1 is the front view of this embodiment;

[0025] Figure 2 is the structural schematic diagram of this embodiment;

[0026] Figure 3 is the front view of the stirring member of this embodiment.

[0027] In the figure: 1, workbench; 2, installation table; 3, heat preservation device; 31, heat preservation box; 311, heat preservation cover; 32, liquid supplement cylinder; 33, stirring member; 4, temperature display; 5, liquid supplement pipe; 6, folding support frame. Detailed Embodiment

[0028] In order to make the content of the present utility model easier to be clearly understood, the present utility model will be further described below according to specific embodiments in conjunction with the accompanying drawings.

[0029] It should be noted that the terms "center", "upper", "lower", "front", "rear", "left", "right", "inner", "outer", etc. used herein indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present utility model. Unless otherwise stated, the meaning of "plurality" is two or more.

[0030] Unless otherwise clearly specified and defined, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0031] As Figures 1 to 3 shown, a cell liquid supplement device includes a workbench 1, an installation table 2 on the workbench 1, and a heat preservation device 3 fixed on the installation table 2. A heater is provided at the bottom of the heat preservation device 3 to heat the liquid supplement.

[0032] Specifically, asFigure 2 As shown in the figure, the heat preservation device 3 includes a heat preservation box 31 for containing heat preservation liquid, a liquid replenishment cylinder 32 for injecting liquid for replenishment, and a stirring member 33; the heat preservation box 31 is fixedly connected to the top surface of the installation table 2, the liquid replenishment cylinder 32 is detachably installed in the heat preservation box 31, and the stirring member 33 is arranged in the liquid replenishment cylinder 32. The heat preservation box 31 of the present utility model can heat and keep warm three liquid replenishment cylinders 32 at the same time. The bottom of the heat preservation box 31 is provided with a heating plate for heating, a temperature controller for controlling the temperature, and a magnetic device for driving the stirring member 33, which can be used for heating the liquid for replenishment, keeping the liquid for replenishment warm, and stirring, preventing the temperature from decreasing during the liquid replenishment process from affecting the growth of cells, and ensuring that various components in the liquid for replenishment can be evenly distributed. The heat preservation box 31 is made of insulating material, and heat preservation liquid can be poured into its box body, which can effectively reduce the heat dissipation of the liquid for replenishment and provide a stable temperature environment for the liquid for replenishment.

[0033] As Figure 3 shown in the figure, the stirring element of the present utility model is oval and made of permanent magnet material, which can effectively respond to the stimulation of the external magnetic field, thereby driving the stirring movement of the liquid, realizing the stirring and mixing of the liquid for replenishment. The surface of the stirring member 33 is wrapped by polypropylene. Through the above settings, it is convenient to clean the stirring member 33 and prevent the stirring member 33 from being corroded, thereby avoiding contaminating the liquid for replenishment.

[0034] As Figure 1 shown in the figure, the heat preservation box 31 of the present utility model is provided with a heat preservation cover 311 at the top, and the heat preservation cover 311 is provided with positioning holes for fixing the liquid replenishment cylinder 32. A snap ring is provided on the outer periphery of the bottom of the heat preservation cover 311, and the snap ring is snap-connected with the outer periphery of the top of the heat preservation box 31. The heat preservation cover 311 can not only be used to fix the liquid replenishment cylinder 32, but also reduce heat dissipation, which is beneficial to the heat preservation of the liquid for replenishment.

[0035] As Figure 2 shown in the figure, the liquid replenishment cylinder 32 of the present utility model is provided with a snap ring at the top, and the snap ring is snap-connected above the positioning hole. Through the above design, the experimenter only needs to insert the liquid replenishment cylinder 32 into the positioning hole to easily fix it, and it is also convenient to remove the liquid for replenishment.

[0036] As Figure 1 shown in the figure, the bottom of the heat preservation box 31 is provided with a temperature display 4, which is convenient for the experimenter to observe the change of temperature. Buttons for adjusting the temperature are also provided on both sides of the display, as well as a power knob for controlling the movement speed of the stirring member 33, which is convenient for the experimenter to adjust the temperature and the operation speed of the stirring member 33 according to needs.

[0037] As Figure 2As shown, a liquid replenishing tube 5 is provided at the bottom of the liquid replenishing cylinder 32. One end of the liquid replenishing tube 5 communicates with the bottom of the liquid replenishing cylinder 32, and the other end passes through the side wall of the incubator 31 and communicates with the outside, facilitating the experimenter to input the liquid in the liquid replenishing cylinder 32 into the cell culture bag. To prevent the heat preservation liquid from leaking out of the incubator, sealing rings are fixed on both sides where the liquid replenishing cylinder 32 passes through the incubator. In addition, a filter screen is provided at one end of the liquid replenishing tube 5 that communicates with the liquid replenishing cylinder 32. The filter screen can remove impurities in the replenishing liquid and also prevent the stirrer from flowing into the liquid replenishing tube 5, causing blockage of the liquid outlet of the liquid replenishing tube 5, facilitating the experimenter to inject the replenishing liquid. A heat preservation sleeve is provided outside the liquid replenishing tube 5 of the present utility model for further heat preservation, preventing the heat of the replenishing liquid from dissipating and the temperature from decreasing, which affects the growth of cells. The liquid replenishing tube 5 of the present utility model is fixed in the waterproof ring on the side wall of the incubator 31, and the waterproof ring and the liquid replenishing tube 5 can be removed from the incubator 31 together, facilitating the experimenter to replace or clean the liquid replenishing tube 5.

[0038] As Figure 2 shown, on the top surface of the workbench 1 of the present utility model, there is a folding support frame 6 for supporting the cell culture bag. The folding support frame 6 includes a rotating plate and an extension plate sleeved at the outer end of the rotating plate. One end of the rotating plate is hinged to the side of the mounting table 2, and the other end is movably connected to the extension plate. The extension plate can be slidably connected along the side of the rotating plate. When using the folding support frame 6, it is necessary to pull out the extension plate so that the extension plate is obliquely fixed on the workbench 1, and then the cell culture bag can be placed on the folding support frame 6. When the folding support frame 6 is not in use, slide the extension plate upward along the side of the rotating plate until the rotating plate is sleeved inside the extension plate, and then fix it to the bottom of the mounting table 2, which is convenient for the experimenter to perform cell liquid replenishing work.

[0039] In addition, when the side wall of the incubator 31 of the present utility model is made of a transparent material, a scale bar is provided on the liquid replenishing cylinder 32, facilitating the experimenter to control the amount of the replenishing liquid poured into the liquid replenishing cylinder 32.

[0040] The above-described embodiments are only the preferred embodiments of the present utility model and cannot be used to limit the protection scope of the present utility model. Any non-substantive changes and modifications made by those skilled in the art based on the present utility model shall fall within the protection scope of the present utility model.

Claims

1. A cell rehydration device, comprising a workbench (1), characterized in that, On the top surface of the workbench (1), an installation platform (2) is fixed. On the top of the installation platform (2), a heat preservation device (3) is fixed. The heat preservation device (3) includes a heat preservation box (31) for containing heat preservation liquid, a liquid replenishment cylinder (32) for injecting replenishment liquid, and a stirring member (33) for stirring; the heat preservation box (31) is fixedly connected to the top surface of the installation platform (2), the liquid replenishment cylinder (32) is detachably installed in the heat preservation box (31), and the stirring member (33) is arranged in the liquid replenishment cylinder (32).

2. The cell rehydration device according to claim 1, wherein On the top of the heat preservation box (31), a heat preservation cover (311) is provided. The heat preservation cover (311) is provided with a positioning hole for fixing the liquid replenishment cylinder (32).

3. The cell rehydration device according to claim 2, characterized in that, On the top of the liquid replenishment cylinder (32), a clamping ring is provided. The clamping ring is clamped in the positioning hole.

4. The cell rehydration device according to claim 1, characterized in that, On the bottom of the heat preservation box (31), a temperature display (4) is provided.

5. The cell rehydration device according to claim 1, characterized in that, On the bottom of the liquid replenishment cylinder (32), a liquid replenishment pipe (5) is provided. One end of the liquid replenishment pipe (5) communicates with the bottom of the liquid replenishment cylinder (32), and the other end passes through the side wall of the heat preservation box (31) to communicate with the outside.

6. The cell rehydration device according to claim 5, characterized in that, A filter screen is provided at one end of the liquid replenishment pipe (5) communicating with the liquid replenishment cylinder (32).

7. The cell rehydration device according to claim 5, wherein, A heat preservation sleeve is provided on the outside of the liquid replenishment pipe (5).

8. The cell rehydration device according to claim 1, characterized in that, The workbench (1) is provided with a folding support frame (6) for support.

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