Cell culture medium dissolution promoting preparation device

Abstract

The utility model discloses a cell culture medium dissolution promoting preparation device which comprises a preparation barrel and a bottom frame, vertical frames are fixedly installed on the top face of the bottom frame, the preparation barrel is rotationally connected between the vertical frames through a rotating shaft, and a driving motor is fixedly installed on the top face of the preparation barrel. The device has the beneficial effects that the main shaft pipe and the side pipe which play a role in heating and stirring are adopted, and the electric heating wires are arranged in the main shaft pipe and the side pipe, so that when a cell culture medium is prepared, raw materials can be put into the preparation barrel, the driving motor drives the driving belt pulley to rotate, and the driven belt pulley is driven to rotate through the connecting belt; meanwhile, the electric heating wire is synchronously started to heat raw materials, lipid substances such as triglyceride are promoted to be dissolved through stirring and heating, the mixing uniformity is improved, meanwhile, the heating position of the electric heating wire located in the side pipe is continuously changed, heating is more uniform, and the mixing effect is better. The dissolving efficiency and the mixing uniformity of cell culture medium preparation are further promoted, and the working efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the field of cell culture medium production equipment, and specifically to a cell culture medium promoting dissolution and preparation device. Background Technique

[0002] Cell engineering is an important aspect of bioengineering. Cell engineering plays an increasingly important role in the fields of life science, agriculture, medicine, food, environmental protection, etc. Cell culture media are widely used within the scope of cell engineering. A cell culture medium is both a basic substance that supplies nutrients to cells and promotes cell reproduction and proliferation in cell culture, and also the living environment for the growth and reproduction of cultured cells. The appearance of a cell culture medium used for cell culture should be a colored, transparent, clear, slightly viscous liquid, without precipitation, particles or flocculates. Therefore, the mixing operation of cell culture media is very important.

[0003] After retrieval, it is found that the application number is CN202122554221.1, and the name is a mixer for preparing cell culture media. This application points out the problems that the equipment for mixing cell culture media in the prior art generally has a single structure and poor mixing effect, which affects the quality of the culture medium. Through an upper liquid leakage plate, an upper stirring roller, a lower liquid leakage plate, and a lower stirring roller, multiple stratified stirrings are provided, improving the stirring efficiency. However, in the actual process of preparing cell culture media, many lipid substances, such as triglycerides, are difficult to dissolve and mix at normal temperature, resulting in difficulty in efficiently mixing and preparing lipid substances when this application is used, affecting the preparation efficiency of the culture medium, and further improvements can be made. At the same time, the stirring and mixing method of this application is relatively single, and the working efficiency can be further improved. In addition, lipid substances are organic substances and are extremely likely to generate foam when stirred in the air, affecting the quality of the culture medium after mixing, and further improvements can also be made.

[0004] In response to the problems in the related art, no effective solution has been proposed yet. Content of the Utility Model

[0005] (1) Technical Problems to be Solved

[0006] In view of the deficiencies of the prior art, the utility model provides a cell culture medium promoting dissolution and preparation device, which has the advantage of improving work efficiency, thereby solving the problems in the above background technique.

[0007] (2) Technical Solutions

[0008] To achieve the above-mentioned advantage of improving work efficiency, the specific technical solutions adopted by the utility model are as follows:

[0009] Cell culture medium solubilization preparation device, including a preparation barrel and a bottom frame. A vertical frame is fixedly installed on the top surface of the bottom frame, and a preparation barrel is rotatably connected between the vertical frames through a rotating shaft. A driving motor is fixedly installed on the top surface of the preparation barrel. A driving pulley is installed at the output end of the driving motor. A main shaft tube is rotatably connected through the axis position of the preparation barrel, and a side tube is fixedly connected through the side wall of the main shaft tube inside the preparation barrel. Electric heating wires are fixedly installed inside the main shaft tube and the side tube. The top surface of the main shaft tube is connected to an external power supply through a slip ring. A driven pulley is fixedly sleeved on the outer surface of the top of the main shaft tube, and the driven pulley is connected to the driving pulley through a connecting belt. A rear frame is fixedly installed on the top surface of the bottom frame behind the preparation barrel, and an electric cylinder is fixedly installed on the back surface of the rear frame. The other end of the moving rod of the electric cylinder is fixedly installed with a top wheel.

[0010] Furthermore, a feeding hopper is connected through the top surface of the preparation barrel, and an upper cover is spirally sleeved at the top opening of the feeding hopper.

[0011] Furthermore, a discharge pipe is connected through the bottom surface of the preparation barrel, and a flap valve is installed on the surface of the discharge pipe.

[0012] Furthermore, multiple groups of side tubes are arranged from top to bottom, and the other end of the side tube is fixedly connected with a scraper.

[0013] Furthermore, the top wheel is in rolling contact with the back surface of the preparation barrel.

[0014] Furthermore, the main shaft tube is rotatably connected to the top surface of the preparation barrel through a sealed bearing, and the main shaft tube and the preparation barrel are coaxially arranged.

[0015] Furthermore, an air extraction pump is fixedly installed on the side wall of the preparation barrel, and the air extraction end of the air extraction pump is connected through an air extraction pipe to the inside of the preparation barrel.

[0016] Furthermore, the outer wall edge of the scraper is in sliding contact with the inner wall of the preparation barrel.

[0017] (III) Beneficial effects

[0018] Compared with the prior art, the present utility model provides a cell culture medium solubilization preparation device, which has the following beneficial effects:

[0019] (1). The present utility model employs a main shaft tube and a side tube that serve the functions of heating and stirring. Electric heating wires are installed inside both the main shaft tube and the side tube. When preparing cell culture medium, raw materials can be placed into the preparation barrel. The driving motor drives the driving pulley to rotate, which drives the driven pulley to rotate through the connecting belt, and then drives the main shaft tube and the side tube to rotate. At the same time, the electric heating wires are started synchronously to heat the raw materials. By stirring and heating, it promotes the dissolution of lipid substances such as triglycerides, improving the uniformity of mixing. At the same time, the electric heating wire in the side tube continuously changes the heating position, making the heating more uniform, further promoting the dissolution efficiency of cell culture medium preparation and the uniformity of mixing, and improving the work efficiency.

[0020] (2). The present utility model employs an electric cylinder and a top wheel. During the heating and stirring process of the main shaft tube and the side tube, the electric cylinder expands and contracts to push the top wheel to move, and the top wheel pushes the preparation barrel to swing reciprocally along the rotating shaft, thus shaking the internal raw materials, further improving the uniformity and speed of mixing, and improving the work efficiency. At the same time, during the mixing process, the air extraction pump is started to extract the excess air inside the preparation barrel, reducing the oxygen content, thereby reducing the foam generated during the stirring of organic substances, improving the yield rate, and further improving the work efficiency. Brief Description of the Drawings

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0022] Figure 1 It is a schematic diagram of the internal structure of the cell culture medium promoting dissolution and preparation device proposed by the present utility model;

[0023] Figure 2 It is the front view of the cell culture medium promoting dissolution and preparation device proposed by the present utility model;

[0024] Figure 3 It is the side view of the cell culture medium promoting dissolution and preparation device proposed by the present utility model;

[0025] Figure 4 It is a schematic diagram of the external structure of the cell culture medium promoting dissolution and preparation device proposed by the present utility model.

[0026] In the figure:

[0027] 1. Preparation barrel; 2. Air extraction pump; 3. Air extraction pipe; 4. Driving motor; 5. Driving pulley; 6. Driven pulley; 7. Electric slip ring; 8. Feeding hopper; 9. Main spindle tube; 10. Side tube; 11. Electric heating wire; 12. Vertical frame; 13. Rotating shaft; 14. Scraper; 15. Discharge pipe; 16. Plug valve; 17. Rear frame; 18. Bottom frame; 19. Electric cylinder; 20. Top wheel; 21. Connecting belt. Detailed implementation manners

[0028] To further illustrate each embodiment, the present utility model provides accompanying drawings, which are a part of the disclosure of the present utility model. These drawings are mainly used to illustrate the embodiments and can be combined with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these contents, those of ordinary skill in the art should be able to understand other possible implementation manners and the advantages of the present utility model. The components in the drawings are not drawn to scale, and similar component symbols are usually used to represent similar components.

[0029] According to an embodiment of the present utility model, a cell culture medium solubilization and preparation device is provided.

[0030] Now, the present utility model will be further described in conjunction with the accompanying drawings and specific implementation manners. As Figures 1-4As shown in the figure, the cell culture medium solubilization and preparation device according to the embodiment of the present utility model includes a preparation barrel 1 and a bottom frame 18. A vertical frame 12 is fixedly installed on the top surface of the bottom frame 18, and a preparation barrel 1 is rotatably connected between the vertical frames 12 through a rotating shaft 13. A driving motor 4 is fixedly installed on the top surface of the preparation barrel 1. A driving pulley 5 is installed at the output end of the driving motor 4. A main shaft tube 9 is rotatably connected through the central axis position of the preparation barrel 1. A side tube 10 is fixedly connected and penetrated through the side wall of the main shaft tube 9 inside the preparation barrel 1. Both the main shaft tube 9 and the side tube 10 are made of hollow metal tubes. Electric heating wires 11 are fixedly installed inside the main shaft tube 9 and the side tube 10. The top surface of the main shaft tube 9 is connected to an external power supply through an electric slip ring 7. The electric slip ring 7 facilitates rotational power supply. A driven pulley 6 is fixedly sleeved on the outer surface of the top of the main shaft tube 9. The driven pulley 6 is connected to the driving pulley 5 through a connecting belt 21. A rear frame 17 is fixedly installed on the top surface of the bottom frame 18 behind the preparation barrel 1. An electric cylinder 19 is fixedly installed on the back surface of the rear frame 17. The other end of the moving rod of the electric cylinder 19 is fixedly installed with a top wheel 20. Electric heating wires 11 are installed inside both the main shaft tube 9 and the side tube 10. When preparing the cell culture medium, raw materials can be put into the preparation barrel 1. The driving motor 4 drives the driving pulley 5 to rotate, drives the driven pulley 6 to rotate through the connecting belt 21, and then drives the main shaft tube 9 and the side tube 10 to rotate. At the same time, the electric heating wires 11 are started synchronously to heat the raw materials, promoting the dissolution of lipid substances such as triglycerides through stirring and heating, improving the mixing uniformity. At the same time, the electric heating wire 11 in the side tube 10 continuously changes the heating position, making the heating more uniform, further promoting the dissolution efficiency of the cell culture medium preparation and the mixing uniformity, improving the work efficiency. At the same time, during the heating and stirring process of the main shaft tube 9 and the side tube 10, the electric cylinder 19 expands and contracts to push the top wheel 20 to move, and the top wheel 20 pushes the preparation barrel 1 to swing back and forth along the rotating shaft 13, thereby shaking the internal raw materials, further improving the mixing uniformity and speed, and improving the work efficiency.

[0031] In one embodiment, a feeding hopper 8 is connected through the top surface of the preparation barrel 1 in a penetrating manner, and an upper cover is spirally sleeved at the top opening of the feeding hopper 8, which is convenient for blocking the feeding hopper 8.

[0032] In one embodiment, a discharge pipe 15 is connected through the bottom surface of the preparation barrel 1 in a penetrating manner, and a plug valve 16 is installed on the surface of the discharge pipe 15. The discharge pipe 15 is convenient for discharging materials.

[0033] In one embodiment, multiple groups of side tubes 10 are arranged from top to bottom, and a scraper 14 is fixedly connected to the other end of the side tube 10. The scraper 14 is convenient for scraping off the raw materials on the inner wall of the preparation barrel 1.

[0034] In one embodiment, the top wheel 20 is in rolling contact with the back surface of the preparation barrel 1, and the friction is reduced through the rolling of the top wheel 20.

[0035] In one embodiment, the main shaft tube 9 is rotatably connected to the top surface of the preparation barrel 1 through a sealed bearing, and the main shaft tube 9 and the preparation barrel 1 are coaxially arranged to improve the stability of rotation.

[0036] In one embodiment, an air extraction pump 2 is fixedly installed on the side wall of the preparation barrel 1, and the air extraction end of the air extraction pump 2 is connected to the inside of the preparation barrel 1 through an air extraction pipe 3. During the mixing process, the air extraction pump 2 is started to extract the excess air inside the preparation barrel 1, reduce the oxygen content, and thus reduce the foam generated during the stirring of organic substances, improve the yield rate, and further improve the working efficiency.

[0037] In one embodiment, the outer wall edge of the scraper 14 is in sliding contact with the inner wall of the preparation barrel 1, and the scraper 14 is made of hard silicone material to avoid scratching the inner wall of the preparation barrel 1.

[0038] Working principle:

[0039] Electric heating wires 11 are installed inside both the main shaft tube 9 and the side tube 10. When preparing the cell culture medium, raw materials can be put into the preparation barrel 1. The driving motor 4 drives the driving pulley 5 to rotate, drives the driven pulley 6 to rotate through the connecting belt 21, and then drives the main shaft tube 9 and the side tube 10 to rotate. At the same time, the electric heating wires 11 are started synchronously to heat the raw materials, promote the dissolution of lipid substances such as triglycerides through stirring and heating, improve the mixing uniformity. At the same time, the electric heating wire 11 in the side tube 10 continuously changes the heating position, making the heating more uniform, further promoting the dissolution efficiency of the cell culture medium preparation and the mixing uniformity, improving the working efficiency. At the same time, during the heating and stirring process of the main shaft tube 9 and the side tube 10, the electric cylinder 19 expands and contracts to push the top wheel 20 to move, and the top wheel 20 pushes the preparation barrel 1 to swing reciprocally along the rotating shaft 13, thus shaking the internal raw materials, further improving the mixing uniformity and speed, and improving the working efficiency. At the same time, during the mixing process, the air extraction pump 2 is started to extract the excess air inside the preparation barrel 1, reduce the oxygen content, and thus reduce the foam generated during the stirring of organic substances, improve the yield rate, and further improve the working efficiency.

[0040] In the present utility model, unless otherwise clearly specified and limited, terms such as "installation", "setting", "connection", "fixation", "rotary connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the internal communication of two components or the interaction relationship between two components. Unless otherwise clearly limited, for those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0041] The above are only preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A cell culture medium solubilization preparation device, characterized in that It includes a preparation barrel (1) and a chassis (18). A vertical frame (12) is fixedly installed on the top surface of the chassis (18). The preparation barrel (1) is rotatably connected between the vertical frames (12) through a rotating shaft (13). A driving motor (4) is fixedly installed on the top surface of the preparation barrel (1). A driving pulley (5) is installed at the output end of the driving motor (4). A main shaft pipe (9) is rotatably connected through the central axis position of the preparation barrel (1). A side pipe (10) is fixedly connected and penetrated through the side wall of the main shaft pipe (9) inside the preparation barrel (1). Electric heating wires (11) are fixedly installed inside the main shaft pipe (9) and the side pipe (10). The top surface of the main shaft pipe (9) is connected to an external power supply through an electric slip ring (7). A driven pulley (6) is fixedly sleeved on the outer surface of the top of the main shaft pipe (9). The driven pulley (6) is connected to the driving pulley (5) through a connecting belt (21). A rear frame (17) is fixedly installed on the top surface of the chassis (18) behind the preparation barrel (1). An electric cylinder (19) is fixedly installed on the back surface of the rear frame (17). The other end of the moving rod of the electric cylinder (19) is fixedly installed with a top wheel (20).

2. The cell culture medium solubilization preparation device according to claim 1, wherein A feeding hopper (8) is connected through the top surface of the preparation barrel (1). An upper cover is spirally sleeved at the top opening of the feeding hopper (8).

3. The cell culture medium solubilization preparation device according to claim 1, wherein A discharge pipe (15) is connected through the bottom surface of the preparation barrel (1). A plug valve (16) is installed on the surface of the discharge pipe (15).

4. The cell culture medium solubilization preparation device according to claim 1, characterized in that, Multiple groups of side pipes (10) are arranged from top to bottom. The other end of the side pipe (10) is fixedly connected with a scraper (14).

5. The cell culture medium solubilization preparation device according to claim 1, wherein, The top wheel (20) is in rolling contact with the back surface of the preparation barrel (1).

6. The cell culture medium solubilization preparation device according to claim 1, characterized in that, The main shaft pipe (9) is rotatably connected to the top surface of the preparation barrel (1) through a sealed bearing, and the main shaft pipe (9) and the preparation barrel (1) are coaxially arranged.

7. The cell culture medium solubilization preparation device according to claim 1, characterized in that, An air extraction pump (2) is fixedly installed on the side wall of the preparation barrel (1). The air extraction end of the air extraction pump (2) is connected through an air extraction pipe (3) to the inside of the preparation barrel (1).

8. The cell culture medium solubilization preparation device according to claim 4, characterized in that, The outer wall edge of the scraper (14) is in sliding contact with the inner wall of the preparation barrel (1).

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