Cell filtering device

Abstract

The utility model provides a cell filtering device, and belongs to the technical field of cell culture filtering. Comprising a filtering supporting body, the filtering supporting body is hollow, a liquid containing groove is formed in the top of the filtering supporting body, a filter screen layer is arranged in the liquid containing groove, a conical liquid collecting hopper is arranged at the bottom of the filtering supporting body, a liquid outlet hole is coaxially formed in the bottom end of the liquid collecting hopper, and the filtering supporting body is coaxially and detachably connected with a filtering test tube. A cell culture solution is dropped into the liquid containing tank through the pipette, is filtered by the filter screen layer, then is dropped into the filter supporting main body, is collected by the liquid collecting hopper and then is discharged from the bottom of the liquid collecting hopper, the bottom of the liquid collecting hopper is aligned to the center in the filter test tube, and the dropped solution is directly gathered at the bottom without contacting with the inner wall of the filter test tube. The effect of keeping the clean degree of the cell culture fluid is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture filtration, in particular to a cell filtration device. Background Art

[0002] There are many types of cell culture media and many cell culture methods. How to use the culture medium correctly and maintain the nutrients of the culture medium to the greatest extent to maintain cell growth is very important for every cell culture worker. The use of culture medium varies according to different types of culture medium, culture methods, and cell types. Most culture media use filter plates with a pore size of 0.1 to 0.2 μm for filtration sterilization, and it has become the development direction of culture medium sterilization. It can minimize the nutrient loss of the culture medium.

[0003] Patent announcement number CN221894966U discloses a filtration device for cell culture, including a filter tube body and a filter mechanism. The filter mechanism includes a limiting ring plate. The diameter of the limiting ring plate is larger than the diameter of the filter test tube. A hand-pinch extension plate is fixedly provided on one side of the limiting ring plate. A filter support frame is fixedly provided below the limiting ring plate. A filter layer is fixedly provided on the inner wall of the filter support frame.

[0004] However, in the filter support of the filtration device, the filtered cell culture fluid tends to flow down along the inner wall of the filter tube. Moreover, when the filter support is too close to the inner wall of the filter tube, the cell culture fluid tends to climb up along the tube wall and the filter support toward the opening of the filter tube due to capillary action, which can easily cause the cell culture fluid to come into contact with contaminants near the tube opening of the filter tube, resulting in contamination of the culture fluid. Utility Model Content

[0005] In view of the above problems, the utility model provides a cell filtration device.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0007] A cell filtration device is provided, comprising a filtration support body, the interior of the filtration support body is hollow, a liquid storage tank is provided on the top of the filtration support body, a plurality of liquid holes communicating with the interior of the filtration support body are hollowed out on the inner wall of the liquid storage tank, a filter mesh layer is provided in the liquid storage tank, the filter mesh layer covers the liquid holes, a conical liquid collecting hopper is provided at the bottom of the filtration support body, a liquid outlet hole is coaxially provided at the bottom end of the liquid collecting hopper, and the filtration support body is coaxially detachably connected to the filtration test tube.

[0008] Furthermore, a communication hole communicating with the interior of the filter support body is formed on the side wall of the filter support body.

[0009] Furthermore, the filtering support body includes an upper cover body and a lower support body, the upper cover body and the lower support body are coaxially threaded, the liquid storage tank is arranged on the upper cover body, the liquid collecting hopper is arranged on the lower support body, and the lower support body is provided with a connecting piece for cooperating with the opening of the filter tube.

[0010] Furthermore, the connecting member includes multiple layers of connecting steps sequentially arranged on the outer wall of the liquid collecting hopper, and the dimensions of the multiple layers of connecting steps gradually decrease in a direction away from the upper cover body.

[0011] Furthermore, a first connecting ring is provided at the bottom of the upper cover body, an inner wall of the first connecting ring is provided with an internal thread, a first external thread matching the internal thread is provided on the outer wall of the lower support body, a second connecting ring is coaxially provided on the top wall of the upper cover body, and a second external thread matching the internal thread is provided on the outer wall of the second connecting ring, and several upper cover bodies can be coaxially stacked.

[0012] Furthermore, a guiding conical surface is provided on the edge of the connecting step.

[0013] Furthermore, expansion and contraction joints are provided on the connecting step, and a plurality of expansion and contraction joints are provided at intervals along the axial direction of the connecting step.

[0014] Furthermore, anti-slip grooves are provided on the outer wall of the upper cover.

[0015] The beneficial effects of the utility model are as follows: when filtering the cell culture fluid, a filter support body with a filter layer of appropriate mesh size is selected, the connecting steps on the lower support body are aligned and connected to the filter test tube, the cell culture fluid is dripped into the liquid storage tank through a pipette, and after the cell culture fluid is filtered by the filter mesh layer, it drips into the interior of the filter support body, is collected by the liquid collecting bucket and then discharged from the bottom of the liquid collecting bucket, the bottom of the liquid collecting bucket is aligned with the center of the interior of the filter test tube, the dripping solution does not touch the inner wall of the filter test tube and directly collects at the bottom, thereby preventing the cell culture fluid from being contaminated by miscellaneous bacteria and impurities on the inner wall near the opening of the filter test tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic cross-sectional structural diagram of the cell filtration device and the filtration tube in the embodiment of the present application in a coordinated state.

[0017] Figure 2 Schematic diagram of the explosion structure of the cell filtration device according to an embodiment of the present application.

[0018] Figure 3 This is a schematic structural diagram of the multi-layer upper cover in the stacked state in an embodiment of the present application.

[0019] Among them, 1. upper cover body; 11. liquid storage tank; 12. filter layer; 13. connecting hole; 14. first connecting ring; 15. second connecting ring; 16. anti-slip groove; 2. lower support body; 21. liquid collecting bucket; 22. liquid outlet; 23. connecting step; 24. first external thread; 25. expansion and contraction joint. DETAILED DESCRIPTION

[0020] In order to better understand the above technical solution, the above technical solution will be described in detail below with reference to the accompanying drawings and specific implementation methods.

[0021] The present application discloses a cell filtration device, referring to Figure 1 、 Figure 2 and Figure 3 The cell filtration device comprises a hollow filter support body. A liquid reservoir 11 is provided on the top of the filter support body. The hollow interior of the liquid reservoir 11 is provided with a plurality of liquid holes connected to the interior of the filter support body. A filter layer 12 is provided within the liquid reservoir 11, covering the liquid holes. The filter layer 12 can be bonded and fixed to the liquid reservoir 11. The filter layer 12 can have different mesh sizes. A cell filtration device with a filter layer 12 of appropriate mesh size is selected based on the filtration requirements of the experiment.

[0022] A connecting hole 13 is provided on the side wall of the filter support body, which is connected to the interior of the filter support body. The connecting hole 13 can maintain the air pressure balance inside the filter support body, allowing the cell solution to smoothly pass through the filter layer 12 and flow downward into the filter tube through the liquid collecting bucket 21.

[0023] A conical liquid collecting hopper 21 is provided at the bottom of the filter support body, the liquid collecting tank 11 and the liquid collecting hopper 21 are spaced apart, a liquid outlet 22 is coaxially provided at the bottom end of the liquid collecting hopper 21, and the filter support body is coaxially detachably connected to the filter test tube. Specifically, the filter support body includes an upper cover body 1 and a lower support body 2, the upper cover body 1 and the lower support body 2 are coaxially threadedly connected, the liquid collecting tank 11 is provided on the upper cover body 1, the liquid collecting hopper 21 is provided on the lower support body 2, and the lower support body 2 is provided with a connector for cooperating with the opening of the filter test tube. The connector includes a plurality of layers of connecting steps 23 sequentially provided on the outer wall of the liquid collecting hopper 21, and the connection between the filter support body and the filter test tube can be completed by interference-fitting the connecting steps 23 into the opening of the filter test tube. The multi-layer steps can adapt to filter test tubes with openings of different sizes, thereby improving the adaptability of the cell filtration device.

[0024] Specifically, the upper cover body 1 and the bottom are provided with a first connecting ring 14, the inner wall of the first connecting ring 14 is provided with an internal thread, the outer wall of the lower support body 2 is provided with a first external thread 24 adapted to the internal thread on the first connecting ring 14, and a second connecting ring 15 is coaxially fixed to the top wall of the upper cover body 1. The outer wall of the second connecting ring 15 is provided with a second external thread adapted to the internal thread on the first connecting ring 14. Multiple upper cover bodies 1 can be coaxially stacked. In the multiple coaxially stacked upper cover bodies 1, the mesh number of the filter layer 12 can be the same, or the mesh number of the filter layer 12 increases from top to bottom, thereby improving the filtration effect of the cell solution or achieving the purpose of graded filtration of the cell solution. The connecting hole 13 is specifically opened on the upper cover body 1. When multiple upper cover bodies 1 are stacked, the air pressure balance in the cell filtration device can also be maintained, allowing the cell solution to be filtered smoothly.

[0025] Furthermore, the edge of the connecting step 23 is provided with a guiding cone, so that when the cell filtration device is connected to the filter tube, the connecting step 23 can accurately match and connect with the filter tube. The connecting step 23 is also provided with expansion and contraction joints 25, and a plurality of expansion and contraction joints 25 are provided along the axial direction of the connecting step 23. The expansion and contraction joints 25 can provide the connecting step 23 with a movable expansion and contraction margin, which facilitates the deformation of the connecting step 23 and the connection with the filter tube. At the same time, the expansion and contraction joints 25 can also keep the air pressure in the filter tube balanced with the external environment, so that the solution in the liquid collecting hopper 21 can flow smoothly into the filter tube.

[0026] Furthermore, anti-slip grooves 16 are provided on the outer wall of the upper cover body 1, which can increase the friction force during operation by the experimenter when the upper cover body 1 is threadedly connected to the lower support body 2 or another upper cover body 1, making the cell filtration device easy to assemble and portable. Those skilled in the art should understand that although the preferred embodiments of the utility model have been described, those skilled in the art can make additional changes and modifications to these embodiments once they understand the basic inventive concept. Therefore, the attached claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the utility model. Obviously, those skilled in the art can make various changes and modifications to the utility model without departing from the spirit and scope of the utility model. Thus, if these modifications and variations of the utility model fall within the scope of the claims of the utility model and their equivalents, the utility model is also intended to include these modifications and variations.

Claims

1. A cell filtration device, characterized in that: The invention comprises a filter support body, wherein the interior of the filter support body is hollow, a liquid storage tank (11) is provided on the top of the filter support body, a plurality of liquid holes communicating with the interior of the filter support body are hollowed out on the inner wall of the liquid storage tank (11), a filter screen layer (12) is provided in the liquid storage tank (11), and the filter screen layer (12) covers the liquid holes, a conical liquid collecting hopper (21) is provided at the bottom of the filter support body, a liquid outlet hole (22) is coaxially provided at the bottom end of the liquid collecting hopper (21), and the filter support body is coaxially detachably connected to the filter test tube.

2. A cell filtration device according to claim 1, characterized in that: A communication hole (13) communicating with the interior of the filter support body is provided on the side wall of the filter support body.

3. A cell filtration device according to claim 2, characterized in that: The filtering support body comprises an upper cover body (1) and a lower support body (2); the upper cover body (1) and the lower support body (2) are coaxially threadedly connected; the liquid storage tank (11) is arranged on the upper cover body (1); the liquid collecting hopper (21) is arranged on the lower support body (2); and the lower support body (2) is provided with a connector for cooperating with the opening of the filtering test tube.

4. A cell filtration device according to claim 3, characterized in that: The connecting member comprises multiple layers of connecting steps (23) sequentially arranged on the outer wall of the liquid collecting hopper (21), and the dimensions of the multiple layers of connecting steps (23) gradually decrease in a direction away from the upper cover body (1).

5. A cell filtration device according to claim 4, characterized in that: The bottom of the upper cover body (1) is provided with a first connecting ring (14), the inner wall of the first connecting ring (14) is provided with an internal thread, the outer wall of the lower support body (2) is provided with a first external thread (24) that matches the internal thread, the top wall of the upper cover body (1) is coaxially provided with a second connecting ring (15), the outer wall of the second connecting ring (15) is provided with a second external thread that matches the internal thread, and a plurality of the upper cover bodies (1) can be coaxially stacked.

6. A cell filtration device according to claim 4, characterized in that: The edge of the connecting step (23) is provided with a guiding conical surface.

7. A cell filtration device according to claim 4, characterized in that: The connecting step (23) is provided with expansion and contraction seams (25), and a plurality of expansion and contraction seams (25) are arranged at intervals along the axial direction of the connecting step (23).

8. A cell filtration device according to any one of claims 3 to 6, characterized in that: Anti-slip grooves (16) are provided on the outer wall of the upper cover (1).

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