Field sampling box for fixing biological sample

Abstract

The utility model discloses a field sampling box for fixing biological samples, which comprises a box body, the box body is detachably connected with a sealing cover, a heat preservation chamber and a normal temperature chamber are arranged in the box body, a first metal block, a second metal block and a third metal block are arranged in the heat preservation chamber, the first metal block is provided with a reagent bottle hole, a plurality of cutter position holes and a plurality of tweezers position holes, and the second metal block is provided with a reagent bottle hole. A vacuum pump is fixedly connected in the normal temperature chamber, the air inlet end of the vacuum pump is communicated with the heat preservation chamber, a plurality of through holes are formed in the box body and formed in the side wall of the normal temperature chamber, and a ventilation pipe is arranged in the heat preservation chamber and extends out of the box body. A tool rack is arranged in the normal temperature chamber, and a temperature measuring rod is arranged in the heat preservation chamber. According to the device, low-temperature preservation of biological samples is achieved through the first metal block, the second metal block and the third metal block which are cooled in advance, meanwhile, vacuumizing can be conducted, and the influence on the biological samples in the sampling process is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological sample sampling boxes, in particular to a field sampling box used for fixing biological samples. Background Art

[0002] Life science research often requires studying animal or plant tissues, cells, and even deeper structural levels. Most natural animal and plant materials must be collected from the field, fixed, brought back to the laboratory, and microscopically prepared for structural observation. Once removed from their original environment, these materials undergo structural and physiological changes. For example, tissue structures may atrophy, die, or decompose, and the prepared specimens may no longer reflect the true state of the organism. Therefore, field samples must be collected quickly, stored at low temperatures, and maintained in a fixed solution for a short soaking time to maximize their preservation of their natural state.

[0003] Currently, most laboratories use ice boxes for field sampling, with the sampling tools and fixative placed in crushed ice to maintain a low temperature. Frequent opening and closing of the ice box during sampling results in significant heat exchange between the inside and outside of the box, affecting sample freshness. Furthermore, low-density samples, such as leaves, floral organs, and fine roots, often float on the surface of the fixative, necessitating vacuum pumping back to the laboratory to evacuate the sample and sink it into the fixative. Failure to evacuate the sample promptly compromises the fixative's effectiveness.

[0004] Therefore, a field sampling box for fixing biological samples is proposed to solve the above problems. Utility Model Content

[0005] The purpose of the utility model is to provide a field sampling box for fixing biological samples to solve the problems existing in the above-mentioned prior art. The biological samples can be kept in a low temperature state after collection, avoiding the use of an ice box, and are convenient for vacuuming.

[0006] To achieve the above-mentioned purpose, the present invention provides the following solution: The present invention provides a field sampling box for fixing biological samples, comprising:

[0007] The box body is detachably connected to a sealing cover, and an insulation chamber and a normal temperature chamber are arranged in the box body. Metal blocks 1, 2 and 3 are arranged in the insulation chamber. The metal block 1 is provided with a reagent bottle hole, a plurality of knife holes and a plurality of tweezers holes. The metal block 2 is provided with a plurality of centrifuge tube holes 1, and the metal block 3 is provided with a plurality of centrifuge tube holes 2. The metal block 2 and the metal block 3 are detachably connected. A vacuum pump is fixedly connected in the normal temperature chamber, and the air inlet end of the vacuum pump is connected with the insulation chamber. The box body is provided with a plurality of through holes, and the through holes are provided on the side wall of the normal temperature chamber. A ventilation pipe is provided in the insulation chamber, and the ventilation pipe extends out of the box body. A blocking cover is provided on the ventilation pipe. A tool rack is provided in the normal temperature chamber, and the tool rack is provided with a plurality of the knife holes and the tweezers holes. A temperature measuring rod is provided in the insulation chamber.

[0008] Preferably, a partition is fixedly connected to the box body, an inner shell is provided in the box body, the inner shell is fixedly connected to the box body and the partition respectively through connecting plates, an insulation layer is fixedly connected between the inner shell, the box body and the partition, the internal space of the inner shell is an insulation room, and the sealing cover is filled with the insulation layer.

[0009] Preferably, a first sealing ring is fixedly connected to the sealing cover, and a second sealing ring is fixedly connected to the box body, the inner shell and the connecting plate, and the first sealing ring and the second sealing ring are correspondingly arranged.

[0010] Preferably, the metal block two and the metal block three are provided with a plurality of connecting holes, connecting rods are inserted into the connecting holes, the ventilation pipe passes through the inner shell, the insulation layer, the partition and the box body, the end of the ventilation pipe is provided with a thread, and the ventilation pipe and the blocking cover are threadedly connected.

[0011] Preferably, two annular grooves are opened in the inner shell, a cover plate is slidably connected in the annular groove, the air inlet end of the vacuum pump is connected to an air inlet pipe, and the air inlet pipe passes through the inner shell, the insulation layer and the partition and penetrates into the insulation chamber.

[0012] Preferably, a fixing seat 1 and a fixing seat 2 are fixedly connected in the inner shell, the metal block 1 is clamped in the fixing seat 1, the metal block 2 is clamped in the fixing seat 2, a fixing plate is fixedly connected to the partition and the side wall of the box, the fixing plate is fixedly connected to a fixing seat 3, and the tool rack is clamped in the fixing seat 3.

[0013] Preferably, the temperature measuring rod is electrically connected to a display screen, the display screen is fixedly connected to the outer side wall of the box, and a handle is rotatably connected to the box.

[0014] Preferably, the knife hole is used to place a scalpel, and a silicone sleeve is provided on the handle of the scalpel.

[0015] The utility model discloses the following technical effects: in this device, the insulation chamber is used to place metal block one, metal block two and metal block three, and the metal block one, metal block two and metal block three need to be frozen and cooled first, and then placed in the insulation chamber after freezing and cooling, and the sealing cover can seal the insulation chamber to reduce the speed of temperature rise in the insulation chamber, the reagent bottle hole on the metal block one is used to place reagent bottles, the knife position hole and tweezers position hole on the metal block one are used to place knives and tweezers, the knife position hole and tweezers position hole on the tool rack are also used to place knives and tweezers, the function of the tool rack is to increase the number of knife position holes and tweezers position holes, the centrifuge tube hole one and the centrifuge tube hole two are used to place centrifuge tubes of different models, the vacuum pump evacuates the insulation chamber to improve the insulation effect of the insulation chamber, and also allows tissue samples to sink into the fixative, the vent pipe is used to ventilate the insulation chamber, and when it is necessary to open the sealing cover, the blocking cover can be opened first to inflate the insulation chamber, and the temperature measuring rod is used to measure the temperature in the insulation chamber. The device achieves low-temperature preservation of biological samples by using the pre-cooled metal blocks 1, 2 and 3, and can also perform vacuum extraction to reduce the impact of the sampling process on the biological samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0017] Figure 1 This is a schematic structural diagram of a field sampling box for fixing biological samples according to the present invention;

[0018] Figure 2 for Figure 1 A partial enlarged schematic diagram;

[0019] Figure 3 This is a top sectional view of the utility model;

[0020] Figure 4 This is a side view of the utility model;

[0021] Figure 5 This is a schematic structural diagram of a metal block according to the present invention;

[0022] Figure 6 This is a schematic diagram of the structure of the scalpel of the utility model Figure 1 ;

[0023] Figure 7 This is a schematic diagram of the structure of the scalpel of the utility model Figure 2 ;

[0024] Among them, 1. Box body; 2. Sealing cover; 3. Insulation chamber; 4. Normal temperature chamber; 5. Metal block 1; 6. Metal block 2; 7. Metal block 3; 8. Reagent bottle hole; 9. Knife hole; 10. Tweezers hole; 11. Centrifuge tube hole 1; 12. Centrifuge tube hole 2; 13. Vacuum pump; 14. Through hole; 15. Vent pipe; 16. Inner shell; 17. Tool rack; 18. Insulation layer; 19. First sealing ring; 20. Second sealing ring; 21. Connecting rod; 22. Annular groove; 23. Cover plate; 24. Inlet pipe; 25. Fixing seat 1; 26. Fixing seat 2; 27. Fixing plate; 28. Display screen; 29. ​​Handle; 30. Scalpel; 31. Plug cover; 32. Temperature measuring rod; 33. Partition; 34. Connecting plate; 35. Silicone sleeve. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0027] Reference Figure 1-7 The utility model provides a field sampling box for fixing biological samples, comprising:

[0028] Box body 1, box body 1 is detachably connected with sealing cover 2, box body 1 is provided with insulation chamber 3 and normal temperature chamber 4, insulation chamber 3 is provided with metal block 1 5, metal block 2 6 and metal block 3 7, metal block 1 5 is provided with reagent bottle hole 8, several knife position holes 9 and several tweezers position holes 10, metal block 2 6 is provided with several centrifuge tube hole 1 11, metal block 3 7 is provided with several centrifuge tube hole 2 12, metal block 2 6 and metal block 3 7 are detachably connected, normal temperature chamber 4 is fixedly connected to a vacuum pump 13, and the air inlet end of the vacuum pump 13 is communicated with the holding chamber 3. A plurality of through holes 14 are provided on the box body 1, and the through holes 14 are provided on the side wall of the normal temperature chamber 4. A vent pipe 15 is provided in the holding chamber 3, and the vent pipe 15 extends out of the box body 1. A blocking cover 31 is provided on the vent pipe 15. A tool rack 17 is provided in the normal temperature chamber 4, and a plurality of knife position holes 9 and a plurality of tweezers position holes 10 are provided on the tool rack 17. A temperature measuring rod 32 is provided in the holding chamber 3.

[0029] In this device, the insulation chamber 3 is used to place metal block 1 5, metal block 2 6 and metal block 3 7. Metal block 1 5, metal block 2 6 and metal block 3 7 need to be frozen and cooled first, and then placed in the insulation chamber 3 after freezing and cooling. The sealing cover 2 can seal the insulation chamber 3 to reduce the rate of temperature rise in the insulation chamber 3. The reagent bottle hole 8 on the metal block 1 5 is used to place reagent bottles. The knife position hole 9 and tweezers position hole 10 on the metal block 1 5 are used to place knives and tweezers. The knife position hole 9 and tweezers position hole 10 on the tool rack 17 are also used to place knives and tweezers. The function of the tool rack 17 is to increase the number of the knife position hole 9 and the tweezers position hole 10. The centrifuge tube hole 1 The housing 11 and the second centrifuge tube hole 12 are used to accommodate centrifuge tubes of different sizes. The first centrifuge tube hole 11 is suitable for 2ml centrifuge tubes, and the second centrifuge tube hole 12 is suitable for 5ml centrifuge tubes. The vacuum pump 13 evacuates the interior of the holding chamber 3 to improve its insulation. The negative pressure also allows the tissue sample to sink into the fixative. The vent tube 15 is used to ventilate the holding chamber 3. When the sealing cover 2 needs to be opened, the blocking cover 31 can be opened first to inflate the holding chamber 3 and restore it to normal pressure. The temperature measuring rod 32 is used to measure the temperature within the holding chamber 3. In this embodiment, the housing 1 and the sealing cover 2 are connected by a hinge. The locking and opening of the housing 1 and the sealing cover 2 are both based on existing technology. The temperature measuring rod 32 in this embodiment is a Yidu instrument brand, model GJD-200LED.

[0030] A further optimized solution is that a partition 33 is fixedly connected to the box body 1, an inner shell 16 is provided in the box body 1, the inner shell 16 is fixedly connected to the box body 1 and the partition 33 respectively through a connecting plate 34, an insulation layer 18 is fixedly connected between the inner shell 16 and the box body 1 and the partition 33, the internal space of the inner shell 16 is the insulation chamber 3, and the sealing cover 2 is filled with the insulation layer 18.

[0031] The partition 33 is used to separate the internal space of the box body 1. The cavity formed by the inner shell 16, the connecting plate 34, the partition 33 and the box body 1 is used to fill the insulation layer 18. There is also an insulation layer 18 in the sealing cover 2 to improve the insulation effect.

[0032] According to a further optimized solution, a first sealing ring 19 is fixedly connected to the sealing cover 2, and a second sealing ring 20 is fixedly connected to the box body 1 and the connecting plate 34. The first sealing ring 19 and the second sealing ring 20 are arranged correspondingly.

[0033] The first sealing ring 19 and the second sealing ring 20 can improve the sealing between the box body 1, the connecting plate 34 and the sealing cover 2. The first sealing ring 19 and the second sealing ring 20 are both in the shape of a Japanese character, and the second sealing ring 20 is fixed on the upper end surface of the box body 1, the connecting plate 34 and the inner shell 16.

[0034] To further optimize the solution, a number of connecting holes are provided on the metal block 2 6 and the metal block 3 7, and connecting rods 21 are inserted into the connecting holes. The ventilation pipe 15 passes through the inner shell 16, the insulation layer 18, the partition 33 and the box body 1. The end of the ventilation pipe 15 is provided with a thread, and the ventilation pipe 15 and the blocking cover 31 are threadedly connected.

[0035] The metal block 2 6 and the metal block 3 7 are arranged up and down, and the connecting rod 21 connects the metal block 2 6 and the metal block 3 7 together, and leaves a certain gap between them to facilitate the accommodation of the centrifuge tube. When placing the centrifuge tube in the metal block 3 7, the metal block 2 6 needs to be removed. The metal block 2 6 and the metal block 3 7 can be replaced in the upper and lower positions, and the threaded connection of the vent pipe 15 and the plug cover 31 is more convenient.

[0036] To further optimize the solution, two annular grooves 22 are opened in the inner shell 16, a cover plate 23 is slidably connected in the annular groove 22, and the air inlet end of the vacuum pump 13 is connected to an air inlet pipe 24, which passes through the inner shell 16, the insulation layer 18 and the partition 33 and extends deep into the insulation chamber 3.

[0037] The two cover plates 23 can cover the insulation chamber 3 to improve the sealing performance of the insulation chamber 3 , and the air inlet pipe 24 facilitates the vacuum pump 13 to evacuate the air.

[0038] Further optimization scheme, the inner shell 16 is fixedly connected with a fixing seat 1 25 and a fixing seat 2 26, the metal block 1 5 is clamped in the fixing seat 1 25, the metal block 2 6 is clamped in the fixing seat 2 26, the partition 33 and the side wall of the box body 1 are fixedly connected with a fixing plate 27, the fixing plate 27 is fixedly connected with the fixing seat 3, and the tool rack 17 is clamped in the fixing seat 3.

[0039] The fixing seat 1 25 is convenient for fixing the metal block 1 5 , the fixing seat 2 26 is convenient for fixing the metal block 2 6 , and the fixing seat 3 is convenient for fixing the tool rack 17 .

[0040] According to a further optimized solution, the temperature measuring rod 32 is electrically connected to a display screen 28 , the display screen 28 is fixedly connected to the outer wall of the box body 1 , and a handle 29 is rotatably connected to the box body 1 .

[0041] The display screen 28 is used to display the temperature. The temperature measuring rod 32 and the display screen 28 are connected by a wire. The handle 29 is convenient for carrying the device.

[0042] According to a further optimized solution, the knife hole 9 is used to place a scalpel 30 , and a silicone sleeve is provided on the handle of the scalpel 30 .

[0043] The scalpel 30 is a conventional medical scalpel. The scalpel 30 has a handle and a blade head. The handle and the blade head are detachable. A silicone sleeve is provided on the handle, which is existing technology and will not be described in detail.

[0044] The working principle of this device is that before use, the metal blocks 1 5, 2 6 and 3 7 need to be frozen and cooled first, and then placed in the preservation chamber 3 after freezing and cooling, and the scalpel 30 and tweezers to be used are placed in the knife hole 9 and tweezers hole 10 on the metal block 1 5 for cooling. The low-temperature knife and tweezers can keep the natural state of the sample when it is alive to the maximum extent. After cutting the biological sample, the biological sample is placed in a centrifuge tube filled with a fixative, and then the corresponding centrifuge tube is placed in the centrifuge tube hole 11 or the centrifuge tube hole 2 12, and the sealing cover 2 is covered. Then, the vacuum pump 13 is used to evacuate the preservation chamber 3. At this time, make sure that the blocking cover 31 is covered on the vent pipe 15; before opening the sealing cover 2, open the blocking cover 31 for ventilation; during field work, the temperature of the metal blocks 1 5, 2 6 and 3 7 is relatively low, which can keep the fixative at a low temperature and keep the natural state of the sample when it is alive to the maximum extent.

[0045] In the description of the present invention, it should be understood that the terms "longitudinal", "transverse", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they should not be understood as limitations on the present invention.

[0046] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements to the technical solutions of the present invention made by ordinary technicians in this field should fall within the scope of protection determined by the claims of the present invention.

Claims

1. A field sampling box for fixing biological samples, characterized in that: include: The box body (1) is detachably connected to a sealing cover (2), and the box body (1) is provided with an insulation chamber (3) and a constant temperature chamber (4), and the insulation chamber (3) is provided with a metal block 1 (5), a metal block 2 (6) and a metal block 3 (7), the metal block 1 (5) is provided with a reagent bottle hole (8), a plurality of knife holes (9) and a plurality of tweezers holes (10), the metal block 2 (6) is provided with a plurality of centrifuge tube holes 1 (11), the metal block 3 (7) is provided with a plurality of centrifuge tube holes 2 (12), the metal block 2 (6) and the metal block 3 (7) are detachably connected, and the constant temperature chamber (4) is fixed. A vacuum pump (13) is fixedly connected, and the air inlet end of the vacuum pump (13) is communicated with the insulation chamber (3). The box body (1) is provided with a plurality of through holes (14), and the through holes (14) are provided on the side wall of the normal temperature chamber (4). A vent pipe (15) is provided in the insulation chamber (3), and the vent pipe (15) extends out of the box body (1). A blocking cover (31) is provided on the vent pipe (15). A tool rack (17) is provided in the normal temperature chamber (4), and the tool rack (17) is provided with a plurality of knife position holes (9) and a plurality of tweezers position holes (10). A temperature measuring rod (32) is provided in the insulation chamber (3).

2. A field sampling box for fixing biological samples according to claim 1, characterized in that: A partition (33) is fixedly connected to the box body (1), an inner shell (16) is provided in the box body (1), the inner shell (16) is fixedly connected to the box body (1) and the partition (33) respectively through a connecting plate (34), a heat-insulating layer (18) is fixedly connected between the inner shell (16), the box body (1) and the partition (33), the internal space of the inner shell (16) is an insulation chamber (3), and the sealing cover (2) is filled with the heat-insulating layer (18).

3. A field sampling box for fixing biological samples according to claim 2, characterized in that: A first sealing ring (19) is fixedly connected to the sealing cover (2), and a second sealing ring (20) is fixedly connected to the box body (1), the inner shell (16) and the connecting plate (34). The first sealing ring (19) and the second sealing ring (20) are correspondingly arranged.

4. A field sampling box for fixing biological samples according to claim 2, characterized in that: The metal block 2 (6) and the metal block 3 (7) are both provided with a plurality of connection holes, and the connection holes are connected with connection rods (21). The ventilation pipe (15) passes through the inner shell (16), the insulation layer (18), the partition (33) and the box body (1). The end of the ventilation pipe (15) is provided with a thread, and the ventilation pipe (15) and the blocking cover (31) are threadedly connected.

5. A field sampling box for fixing biological samples according to claim 2, characterized in that: Two annular grooves (22) are provided in the inner shell (16), a cover plate (23) is slidably connected in the annular groove (22), an air inlet end of the vacuum pump (13) is connected to an air inlet pipe (24), and the air inlet pipe (24) passes through the inner shell (16), the thermal insulation layer (18) and the partition (33) and penetrates into the thermal insulation chamber (3).

6. A field sampling box for fixing biological samples according to claim 2, characterized in that: A fixing seat 1 (25) and a fixing seat 2 (26) are fixedly connected in the inner shell (16); the metal block 1 (5) is clamped in the fixing seat 1 (25); the metal block 2 (6) is clamped in the fixing seat 2 (26); a fixing plate (27) is fixedly connected to the partition (33) and the side wall of the box body (1); the fixing plate (27) is fixedly connected to the fixing seat 3; and the tool rack (17) is clamped in the fixing seat 3.

7. A field sampling box for fixing biological samples according to claim 2, characterized in that: The temperature measuring rod (32) is electrically connected to a display screen (28), the display screen (28) is fixedly connected to the outer wall of the box body (1), the temperature measuring rod (32) is fixedly connected to the inner shell (16), and a handle (29) is rotatably connected to the box body (1).

8. The field sampling box for fixing biological samples according to claim 1, characterized in that: The knife hole (9) is used to place a scalpel (30), and a silicone sleeve (35) is sleeved on the handle of the scalpel (30).

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