13CO2 isotope labeling assimilation box for wheat pot experiment

Abstract

The utility model relates to the technical field of ecological system carbon circulation, and discloses a 13CO2 isotope labeling assimilation box for a wheat pot experiment, which comprises a bottom plate, a mounting plate mounted at the top end of the bottom plate, a mounting groove formed in the top end of the mounting plate, and a box body arranged above the bottom plate in a penetrating manner and inserted into the mounting groove, locking units connected with the box body are symmetrically arranged outside the mounting plate, a vent hole is formed in the middle of the top end of the box body, a communicating pipe communicated with the vent hole is arranged at the top end of the box body, a valve is installed on the communicating pipe, and a containing container located in the box body is arranged in the middle of the top end of the bottom plate; according to the 13CO2 isotope labeling box, the box body and the mounting plate are mounted in an inserting manner and are matched with the locking unit, so that the box body is fastened, the sealing performance of the box body is effectively improved, the sealing effect is further improved by matching with the design of the sealing gasket, and the accuracy of 13CO2 isotope labeling is further improved.

Description

Technical Field

[0001] The utility model relates to the technical field of ecosystem carbon cycle, in particular to a method for wheat pot test. 13 CO2 isotope labeling assimilation chamber. Background Art

[0002] Carbon (C) is both a fundamental element of life and a fundamental component closely linked to the atmospheric environment and global climate change. Photosynthetically fixed carbon in plants is the starting point for carbon input into the atmosphere-plant-soil system and the fundamental source of organic carbon in plants and soils. Understanding the distribution, utilization, and transformation of photosynthetically fixed carbon is a key scientific issue in the ecosystem carbon cycle and fundamental for accurately assessing ecosystem carbon source / sink functions and developing scientifically sound ecosystem management measures. Isotope feeding-tracing technology, due to its non-destructive nature, high sensitivity, and quantitative specificity, has been widely used to study the distribution and transformation characteristics of photosynthetic carbon in plants.

[0003] Currently, the devices used for isotope labeling are mostly completed through handmade assimilation boxes. The bottom of the assimilation box is often not sealed, which will cause the isotope labeling gas inside to flow out and the external gas to flow in, thereby reducing the labeling efficiency and resulting in low accuracy of the obtained data. Utility Model Content

[0004] In order to solve the technical problem that the bottom of the assimilation box is often not sealed, which will cause the isotope labeled gas inside it to flow out and the outside gas to flow in, thereby reducing the labeling efficiency and resulting in low accuracy of the obtained data, the utility model provides a method for wheat pot test. 13 CO2 isotope labeling assimilation chamber.

[0005] The utility model adopts the following technical solutions to achieve: a method for wheat pot test 13 A CO2 isotope labeling assimilation box includes a bottom plate, a mounting plate is installed on the top of the bottom plate, a mounting groove is provided on the top of the mounting plate, a box body inserted into the mounting groove is provided above the bottom plate, a locking unit connected to the box body is symmetrically provided on the outside of the mounting plate, a vent is provided at the middle position of the top of the box body, a connecting pipe connected to the vent is provided at the top of the box body, a valve is installed on the connecting pipe, a placement container is provided inside the box body at the middle position of the top of the bottom plate, a clamping piece connected to the placement container is symmetrically provided on the inner wall of the mounting plate, a temperature detector and a CO2 concentration detector are installed on one side of the inner wall of the box, and symmetrically arranged blower mechanisms are installed on both sides of the inner wall of the box.

[0006] As a further improvement of the above solution, the clamping member includes an electric push rod installed on the inner wall of the mounting plate, the output shaft of the electric push rod is connected to the clamping plate, and the clamping plate is in contact with the outer wall of the placement container.

[0007] As a further improvement of the above solution, a sealing gasket located at the top of the mounting plate is fixedly connected to the inner wall of the box body, and the mounting plate and the sealing gasket are both rectangular structures.

[0008] As a further improvement of the above scheme, the locking unit includes a fixed rod fixed to the middle position of the outer wall of the mounting plate, a pull plate is slidably sleeved on the fixed rod, a spring connected to the mounting plate and the side wall of the pull plate is sleeved on the fixed rod, a plug-in rod is symmetrically installed on the side of the pull plate close to the mounting plate, and plug-in slots are provided on the mounting plate and the box body, and one end of the plug-in rod extends into the inside of the plug-in slot.

[0009] As a further improvement of the above-mentioned solution, the hair dryer mechanism includes a groove opened on the inner wall of the box, a stabilizing rod is fixedly connected to the inside of the groove, an external sliding sleeve of the stabilizing rod is provided with a sliding seat, a slide sliding on the inner wall of the box is fixed to one side of the sliding seat, a fan is installed on one side of the slide, a lifting part is provided on the fan, and the width of the slide is greater than the width of the groove.

[0010] As a further improvement of the above-mentioned solution, the lifting member includes a stop plate fixed to the inner wall of the box and located below the fan, a cam located above the stop plate is fixedly sleeved on the fan output shaft, the outer wall of the long end of the cam is in sliding contact with the top surface of the stop plate, and the top and bottom ends of the slide are provided with spring 2 which is sleeved on the outside of the stabilizer bar.

[0011] Compared with the prior art, the beneficial effects of the present invention are:

[0012] The utility model adopts a plug-in installation method for the box body and the mounting plate, and cooperates with the function of the locking unit to achieve the fastening of the box body, effectively improving the sealing performance of the box body, and cooperates with the design of the sealing gasket to further improve the sealing effect, thereby providing a 13 CO2 isotope labeling improves accuracy;

[0013] The design of the blowing mechanism makes it easy for the gases to blow against each other in the box, and can also move up and down during the blowing process, effectively increasing the blowing range, thereby accelerating the gas mixing speed and providing convenience for wheat isotope feeding. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0015] Figure 2 for Figure 1 The main cross-section of

[0016] Figure 3 for Figure 2 lateral cross-section of

[0017] Figure 4 is a top sectional schematic diagram of the locking unit;

[0018] Figure 5 for Figure 2 Schematic diagram of the structure enlarged at point A.

[0019] Description of main symbols:

[0020] 1. Base plate; 2. Mounting plate; 3. Box body; 4. Mounting slot; 5. Vent hole; 6. Connecting pipe; 7. Valve; 8. Placement container; 9. Temperature detector; 10. CO2 concentration detector; 11. Fixing rod; 12. Pull plate; 13. Spring 1; 14. Connecting rod; 15. Connecting slot; 16. Electric push rod; 17. Clamping plate; 18. Groove; 19. Slide seat; 20. Slide plate; 21. Fan; 22. Stop plate; 23. Cam; 24. Stabilizer bar; 25. Spring 2; 26. Sealing gasket. DETAILED DESCRIPTION

[0021] Below, the present invention is further described in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0022] Please combine Figures 1 to 5 , a method for wheat pot test in this embodiment 13 The CO2 isotope labeling assimilation box includes a bottom plate 1, a mounting plate 2 is installed on the top of the bottom plate 1, a mounting groove 4 is opened on the top of the mounting plate 2, a box body 3 is inserted into the mounting groove 4 above the bottom plate 1, a locking unit connected to the box body 3 is symmetrically provided on the outside of the mounting plate 2, a vent hole 5 is opened at the middle position of the top of the box body 3, a connecting pipe 6 connected to the vent hole 5 is provided at the top of the box body 3, a valve 7 is installed on the connecting pipe 6, a placement container 8 is provided inside the box body 3 at the middle position of the top of the bottom plate 1, and a clamping device connected to the placement container 8 is symmetrically provided on the inner wall of the mounting plate 2. A temperature detector 9 and a CO2 concentration detector 10 are installed on one side of the inner wall of the box body 3, and symmetrically arranged air blowing mechanisms are installed on both sides of the inner wall of the box body 3; the design of the air vent 5 and the connecting pipe 6 facilitates the introduction of the marking gas into the box body 3, and the design of the valve 7 facilitates the opening and closing of the introduced gas. The temperature detector 9 can detect the temperature inside the box body 3, and the CO2 concentration detector 10 can detect the CO2 concentration inside the box body 3. The model of the temperature detector 9 is testo810, and the model of the CO2 concentration detector 10 is HIMA AR8200.

[0023] The clamping member includes an electric push rod 16 installed on the inner wall of the mounting plate 2. The output shaft of the electric push rod 16 is connected to a clamping plate 17, and the clamping plate 17 contacts the outer wall of the placement container 8. The placement container 8 can be fixed by the clamping member, thereby improving the stability of the placement container 8.

[0024] The inner wall of the box body 3 is fixed with a sealing gasket 26 located at the top of the mounting plate 2. The mounting plate 2 and the sealing gasket 26 are both rectangular structures. The bottom plate 1, the mounting plate 2 and the box body 3 are all made of organic glass with a light transmittance greater than 90%. The box body 3 and the mounting plate 2 are plugged into each other to improve the sealing performance of the inside of the box body 3. The design of the sealing gasket 26 further improves the sealing effect, thereby providing a 13 CO2 isotope labeling improves accuracy.

[0025] The locking unit includes a fixing rod 11 fixed to the middle position of the outer wall of the mounting plate 2, a pull plate 12 is slidably sleeved on the fixing rod 11, a spring 13 connected to the mounting plate 2 and the side wall of the pull plate 12 is sleeved on the fixing rod 11, and a plug-in rod 14 is symmetrically installed on the side of the pull plate 12 close to the mounting plate 2. Plug-in grooves 15 are provided on the mounting plate 2 and the box body 3, and one end of the plug-in rod 14 extends to the inside of the plug-in groove 15; through the design of the locking unit, the box body 3 is opened to facilitate the placement of wheat in the placement container 8, and then the box body 3 is inserted into the mounting groove 4 to achieve the fixation of the box body 3 and the mounting plate 2, thereby providing convenience for wheat testing.

[0026] The air blowing mechanism includes a groove 18 opened on the inner wall of the box body 3, a stabilizing rod 24 is fixedly connected to the inside of the groove 18, and an external sliding sleeve of the stabilizing rod 24 is provided with a slide seat 19. One side of the slide seat 19 is fixedly connected to a slide plate 20 that slides on the inner wall of the box body 3. A fan 21 is installed on one side of the slide plate 20, and a lifting part is provided on the fan 21. The width of the slide plate 20 is greater than the width of the groove 18. By designing two sets of air blowing mechanisms, they can blow against each other, which can accelerate the flow of gas inside the box body 3 and thereby accelerate the mixing of the gas.

[0027] The lifting member includes a stop plate 22 fixed to the inner wall of the box body 3 and located below the fan 21. A cam 23 located above the stop plate 22 is fixedly sleeved on the output shaft of the fan 21. The outer wall of the long end of the cam 23 is in sliding contact with the top surface of the stop plate 22. The top and bottom ends of the slide 19 are provided with a spring 25 sleeved on the outside of the stabilizing rod 24. Through the design of the lifting member, it is convenient to lift and lower during the blowing process inside the box body 3, thereby increasing the blowing range and further accelerating the gas mixing speed.

[0028] A method for wheat pot test in the embodiment of the present application 13The implementation principle of the CO2 isotope labeling assimilation box is as follows: pull the pull plate 12 outward, the pull plate 12 will slide on the fixed rod 11, and the spring 13 will be stretched, and the pull plate 12 will pull the plug rod 14 out of the plug slot 15, then transplant the wheat into the placement container 8, and start the electric push rod 16, the electric push rod 16 will drive the clamping plate 17 to move, so that the two clamping plates 17 can clamp the placement container 8, and then insert the box body 3 into the installation slot 4 in the installation plate 2, so that the sealing gasket 26 on the inner wall of the box body 3 is in close contact with the installation plate 2. At the top, the pull plate 12 is then released. Under the force of the spring 13, the pull plate 12 drives the plug rod 14 to be inserted into the plug slot 15, thereby fastening the mounting plate 2 and the box body 3. This further realizes that the bottom plate 1, the mounting plate 2 and the box body 3 form a sealed space, effectively providing accuracy for isotope labeling. Then, the isotope labeling gas to be injected is connected to the connecting pipe 6, and the isotope labeling gas is turned on and off by the valve 7 controller. The CO2 concentration inside the box body 3 is monitored in real time by the temperature detector 9 and the CO2 concentration detector 10.

[0029] At the same time, the fan 21 is made to work, and the two fans 21 blow against each other, which can speed up the flow rate of the gas inside the box 3, and thus make the gas inside the box 3 evenly mixed. The fan 21 will drive the cam 23 to rotate when working. When the long end of the cam 23 rotates to face downward, the outer wall of the cam 23 will contact the top of the stop plate 22, and then due to the action of the stop plate 22, the cam 23, the fan 21, and the slide 20 will be pushed up, and the slide 19 will slide upward on the stabilizing rod 24, effectively realizing the lifting and lowering of the fan 21, and then facilitating the change of the blowing range of the fan 21, effectively speeding up the gas mixing speed, and then facilitating the isotope gas feeding of wheat. Due to the closure of the box 3, the isotope labeling is more sufficient and the results are more accurate.

[0030] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and replacements made by technicians in this field on the basis of the present invention fall within the scope of protection required by the present invention.

Claims

1. A method for wheat pot experiment 13 The CO2 isotope labeling assimilation chamber is characterized by: It includes a base plate, a mounting plate is installed on the top of the base plate, a mounting groove is provided on the top of the mounting plate, a box body inserted into the mounting groove is provided above the base plate, a locking unit connected to the box body is symmetrically provided on the outside of the mounting plate, a vent is provided in the middle position of the top of the box body, a connecting pipe connected to the vent is provided at the top of the box body, a valve is installed on the connecting pipe, a placement container located inside the box body is provided in the middle position of the top of the base plate, a clamping piece connected to the placement container is symmetrically provided on the inner wall of the mounting plate, a temperature detector and a CO2 concentration detector are installed on one side of the inner wall of the box, and symmetrically arranged blower mechanisms are installed on both sides of the inner wall of the box.

2. The method for wheat pot test according to claim 1 13 The CO2 isotope labeling assimilation chamber is characterized by: The clamping member comprises an electric push rod installed on the inner wall of the mounting plate, the output shaft of the electric push rod is connected with the clamping plate, and the clamping plate is in contact with the outer wall of the placement container.

3. The method for wheat pot test according to claim 1 13 The CO2 isotope labeling assimilation chamber is characterized by: The inner wall of the box body is fixedly connected with a sealing gasket located at the top of the mounting plate, and both the mounting plate and the sealing gasket are rectangular structures.

4. The method for wheat pot test according to claim 1 13 The CO2 isotope labeling assimilation chamber is characterized by: The locking unit includes a fixed rod fixed to the middle position of the outer wall of the mounting plate, a pull plate is slidably sleeved on the fixed rod, a spring connected to the mounting plate and the side wall of the pull plate is sleeved on the fixed rod, a plug-in rod is symmetrically installed on the side of the pull plate close to the mounting plate, and plug-in slots are provided on the mounting plate and the box body, and one end of the plug-in rod extends into the inside of the plug-in slot.

5. The method for wheat pot test according to claim 1 13 The CO2 isotope labeling assimilation chamber is characterized by: The hair dryer mechanism includes a groove opened on the inner wall of the box, a stabilizing rod is fixedly connected to the inside of the groove, an external sliding sleeve of the stabilizing rod is provided with a sliding seat, one side of the sliding seat is fixed with a slide that slides on the inner wall of the box, a fan is installed on one side of the slide, and a lifting part is provided on the fan. The width of the slide is greater than the width of the groove.

6. The method for wheat pot test according to claim 5 13 The CO2 isotope labeling assimilation chamber is characterized by: The lifting member includes a stop plate fixed to the inner wall of the box and located below the fan. A cam located above the stop plate is fixedly sleeved on the fan output shaft. The outer wall of the long end of the cam is in sliding contact with the top surface of the stop plate. The top and bottom ends of the slide are provided with spring 2 which is sleeved on the outside of the stabilizer bar.

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