Cell screening device for stem cells

Abstract

The utility model relates to the technical field of cell screening, in particular to a stem cell screening device which comprises a shell, a base is arranged at the lower end of the interior of the shell, the bottom of the base is fixedly assembled with the inner wall face of the shell through screws, a built-in groove is formed in the base, and a vibration frame is tightly attached to the interior of the built-in groove in a sliding mode. The vibration frame is of an inverted T-shaped structure, a storage base is arranged at the upper end of the vibration frame, a vessel is slidably plugged into a groove of the storage base, and first springs are arranged at the two ends of a built-in groove of the base. Through the driving of the motor, the rotating shaft can drive the eccentric wheel to rotate, so that the far end and the near end of the eccentric wheel are in continuous contact with the vibration frame, and the vibration frame can drive the vessel in the storage seat to vibrate in a reciprocating manner through the sliding of the vibration frame in the built-in groove of the base and the compression of the first spring to reset; therefore, cell substances in the vessel can be more uniformly dispersed when a coloring agent is dropwise added, and the coloring uniformity is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell screening, in particular to a cell screening device for stem cells. Background Art

[0002] Stem cell screening refers to the separation of cells with stem cell characteristics from a variety of cells through a series of biological and genetic experimental methods.

[0003] These cells have the ability to proliferate and self-renew indefinitely and can differentiate into a variety of different cell types. The main purpose of stem cell screening is to study and treat various diseases, as well as to use them in the fields of tissue engineering and regenerative medicine.

[0004] When screening for stem cells, scientists typically look for specific markers, which are proteins or gene expression products unique to stem cells.

[0005] The screening process may include the following steps:

[0006] Cell culture: First, potential stem cell samples are cultured to observe whether they have the ability to self-replicate and multidirectionally differentiate;

[0007] Marker detection: Detect specific markers on the cell surface or inside through immunofluorescence staining, flow cytometry, and other methods;

[0008] Molecular biology testing: Analyze the gene expression profile and protein products of cells through PCR and other technologies;

[0009] Functional experiments: By implanting cells into animal models, we can see whether these cells can restore the function of damaged tissues or organs;

[0010] Whole-genome analysis: Use technologies such as high-throughput sequencing to comprehensively analyze the cell genome to understand its genetic stability and potential differentiation capacity.

[0011] Currently, when stem cells are screened, they need to be subjected to immunofluorescence staining so that they can be better distinguished during screening. During immunofluorescence staining, most of the time, manual shaking is performed after adding the dye. However, the frequency of manual shaking is inconsistent, which greatly reduces the mixing uniformity and adhesion of the dye and cells. Utility Model Content

[0012] The purpose of the present invention is to solve the above-mentioned shortcomings in the prior art and to propose a cell screening device for stem cells.

[0013] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0014] The cam is secured to the upper edge of the base and secured to the lower edge of the base with a spring which is secured to the bottom edge of the base. The cam is secured to the lower edge of the base with a spring which is secured to the bottom edge of the base. The cam is secured to the lower edge of the base with a spring which is secured to the bottom edge of the base.

[0015] In detail, a limiting groove is provided on the inner wall surface of the base, and limiting blocks are provided on both sides of the lower end of the vibration frame. The ends of the limiting blocks are fixed to the surface of the vibration frame by screws, and the surface of the limiting blocks is slidingly and tightly attached to the inner wall surface of the limiting groove.

[0016] In detail, a sealing plate is provided through the upper end of the shell, and the connection position between the sealing plate and the shell is fixed by welding. A feeding pipe is provided through the middle position inside the sealing plate, and the connection position between the feeding pipe and the sealing plate is fixed by resin glue.

[0017] In detail, a micro booster pump is provided above the sealing plate, the outer wall of the micro booster pump is fixedly assembled with the surface of the sealing plate through a bracket, the discharge end flange of the micro booster pump is docked with a valve, and the discharge end of the valve and the feed pipe are fixed to each other by flanges.

[0018] In detail, square rods are provided on both sides of the storage seat, one end of the square rod is fixed to the surface of the storage seat by screws, and the other end of the square rod is welded with an anti-slip plate.

[0019] In detail, a square sleeve is slidably sleeved on the surface of the square rod, a clamping seat is fixedly sleeved on the outer wall of the square sleeve, and the inner side of the clamping seat is covered with the end of the container.

[0020] In detail, a second spring is wound around the surface of the square rod, and two ends of the second spring are fixedly assembled with the surface of the clamping seat and the surface of the anti-slip plate respectively.

[0021] In detail, a door is provided on the front of the shell, the port of the door is covered with a door panel, the lower end of the door panel is rotatably installed with the front of the shell through a door hinge, the upper end of the door panel is opened and closed with the door position of the shell through a door lock, and a handle is fixed on the outward side of the door panel by screws.

[0022] The design scheme proposed by the utility model has the following beneficial effects during application:

[0023] 1. The motor drives the rotating shaft to rotate the eccentric wheel, so that the distal and proximal ends of the eccentric wheel are constantly in contact with the vibration frame. The vibration frame slides in the built-in groove of the base, and the first spring is compressed and reset, so that the vibration frame can vibrate back and forth with the vessel in the storage seat, so that the cell material in the vessel can be more evenly dispersed when the dye is added, thereby improving the uniformity of dyeing.

[0024] 2. Through the distribution of the clamping seats on both sides of the storage seat and the compression and reset of the second spring, the clamping seats on both sides can clamp and position the container in the storage seat from both ends, thereby improving the safety and stability of the container during oscillation. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0026] Figure 2 This is a schematic diagram of the overall structure of the utility model from an oblique top view;

[0027] Figure 3 This is a schematic diagram of the internal structure of the utility model;

[0028] Figure 4 This is an enlarged schematic diagram of point b of the present utility model;

[0029] Figure 5 This is an enlarged schematic diagram of point a of the present invention.

[0030] In the figure: 10. Shell; 11. Base; 12. Vibration frame; 13. Storage seat; 14. Container; 15. First spring; 16. Motor; 17. Eccentric wheel; 20. Limiting groove; 21. Limiting block; 30. Closing plate; 31. Feeding pipe; 32. Micro booster pump; 33. Valve; 40. Square rod; 41. Square sleeve; 42. Clamp seat; 43. Anti-slip plate; 44. Second spring; 50. Door panel; 51. Handle. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0032] Reference Figure 1-Figure 5, a cell screening device for stem cells, including a shell 10, a base 11 is provided at the lower end of the interior of the shell 10, and the bottom of the base 11 is fixedly assembled with the inner wall surface of the shell 10 by screws, a built-in groove is provided inside the base 11, and the interior of the built-in groove is slidably and tightly fitted with a vibration frame 12, the vibration frame 12 is an inverted T-shaped structure, and a storage seat 13 is provided at the upper end of the vibration frame 12, a vessel 14 is slidably inserted into the groove of the storage seat 13, a first spring 15 is provided at both ends of the built-in groove of the base 11, and the two ends of the first spring 15 are respectively fixedly assembled with the inner wall surface of the built-in groove and the end of the vibration frame 12, a motor 16 is provided on the upper end surface of the base 11, and the outer The wall surface is fixedly assembled with the surface of the base 11 through the bracket, and a rotating shaft for driving is provided inside the motor 16, and the end of the rotating shaft extends upward through the interior of the motor 16, and an eccentric wheel 17 is provided at the end of the rotating shaft of the motor 16, and the lower end surface of the eccentric wheel 17 is fixedly assembled with the end of the rotating shaft by screws, and the distal and proximal ends of the eccentric wheel 17 are always in sliding contact with the surface of the vibration frame 12. The motor 16 is a micro motor and is powered by a wire connected to the indoor 220V power socket. When the motor 16 drives the eccentric wheel 17 to rotate, it can ensure that the vessel 14 has a small reciprocating oscillation effect, thereby preventing the mixture of cell material and dye from being spilled while also ensuring the uniformity of mixing.

[0033] It should be further explained that a limiting groove 20 is provided on the inner wall surface of the base 11, and limiting blocks 21 are provided on both sides of the lower end of the vibration frame 12. The ends of the limiting blocks 21 are fixed to the surface of the vibration frame 12 by screws, and the surface of the limiting blocks 21 are slidingly and tightly attached to the inner wall surface of the limiting groove 20, which can ensure that the amplitude of movement of the vibration frame 12 in the built-in groove of the base 11 is limited, thereby ensuring the stability of the oscillation of the vessel 14.

[0034] It should be further explained that a sealing plate 30 is provided through the upper end of the shell 10, and the connection position between the sealing plate 30 and the shell 10 is fixed by welding. A feeding pipe 31 is provided through the middle position inside the sealing plate 30, and the connection position between the feeding pipe 31 and the sealing plate 30 is fixed by resin glue.

[0035] It should be further explained that a micro booster pump 32 is provided above the sealing plate 30. The outer wall surface of the micro booster pump 32 is fixedly assembled to the surface of the sealing plate 30 through a bracket. The discharge end flange of the micro booster pump 32 is connected to a valve 33. The discharge end of the valve 33 and the feed pipe 31 are flange-fixed to each other. The feed end of the micro booster pump 32 is connected to the external dye solvent container through a pipeline, which can realize stable delivery of the dye.

[0036] It should be further explained that square rods 40 are provided on both sides of the storage seat 13, one end of the square rod 40 is fixed to the surface of the storage seat 13 by screws, and the other end of the square rod 40 is welded with an anti-slip plate 43.

[0037] It should be further explained that a square sleeve 41 is slidably sleeved on the surface of the square rod 40, and a clamp seat 42 is fixedly sleeved on the outer wall of the square sleeve 41. The inner side of the clamp seat 42 is covered with the end of the vessel 14, which can improve the oscillation stability of the vessel 14 from both sides.

[0038] It should be further explained that a second spring 44 is wound around the surface of the square rod 40 , and both ends of the second spring 44 are fixedly assembled with the surface of the clamping seat 42 and the surface of the anti-slip plate 43 respectively.

[0039] It should be further explained that a door is provided on the front of the shell 10, and the port of the door is covered with a door panel 50. The lower end of the door panel 50 is rotatably installed with the front of the shell 10 through a door hinge, and the upper end of the door panel 50 is opened and closed with the door position of the shell 10 through a door lock. The outward side of the door panel 50 is fixed with a handle 51 by screws.

[0040] Working method: When cell staining is required for cell screening, the processed cells are uniformly placed in the vessel 14, the vessel 14 is placed in the storage seat 13, and the clamping seats 42 on both sides are pulled so that the vessel 14 can be completely placed in the storage seat 13. Through the reset effect of the second spring 44, the clamping seats 42 on both sides can cover the ends of the vessel 14, thereby achieving solid positioning.

[0041] The micro booster pump 32 is connected to an external dye delivery pipeline, and the dye can be dripped into the container 14 through the feeding pipe 31 in coordination with the opening and closing of the valve 33 .

[0042] In order to improve the contact effect between the dye and the cell material, the power supply of the motor 16 is turned on, so that the power supply 16 can rotate the eccentric wheel 17. The distal and proximal ends of the eccentric wheel 17 are constantly in contact with the vibration frame 12. In conjunction with the compression and reset of the first spring 15, the vibration frame 12 can be moved back and forth in the built-in groove of the base 11, thereby driving the cell material in the vessel 14 to oscillate slightly, thereby improving the mixing effect of the cell material and the dye.

[0043] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cell screening device for stem cells, comprising a housing (10), characterized in that: The lower end of the interior of the shell (10) is provided with a base (11), and the bottom of the base (11) is fixedly assembled with the inner wall surface of the shell (10) by screws. The interior of the base (11) is provided with a built-in groove, and the interior of the built-in groove is slidably and tightly fitted with a vibration frame (12). The vibration frame (12) is an inverted T-shaped structure, and the upper end of the vibration frame (12) is provided with a storage seat (13), and a container (14) is slidably inserted into the groove of the storage seat (13). Both ends of the built-in groove of the base (11) are provided with a first spring (15), and the two ends of the first spring (15) are respectively in contact with the inner wall surface. The inner wall surface of the groove and the end of the vibration frame (12) are fixedly assembled, the upper end surface of the base (11) is provided with a motor (16), and the outer wall surface of the motor (16) is fixedly assembled with the surface of the base (11) through a bracket, the interior of the motor (16) is provided with a rotating shaft for driving, and the end of the rotating shaft passes through the interior of the motor (16) and extends upward, the end of the rotating shaft of the motor (16) is provided with an eccentric wheel (17), and the lower end surface of the eccentric wheel (17) is fixedly assembled with the end of the rotating shaft through a screw, and the distal end of the eccentric wheel (17) is always in sliding contact with the surface of the vibration frame (12).

2. The stem cell screening device according to claim 1, characterized in that: A limiting groove (20) is provided on the inner wall surface of the base (11), and limiting blocks (21) are provided on both sides of the lower end of the vibration frame (12). The ends of the limiting blocks (21) are fixedly assembled with the surface of the vibration frame (12) by screws, and the surface of the limiting blocks (21) is slidingly and tightly arranged with the inner wall surface of the limiting groove (20).

3. The stem cell screening device according to claim 1, characterized in that: A sealing plate (30) is provided through the upper end of the shell (10), and the connection position between the sealing plate (30) and the shell (10) is fixed by welding. A feeding pipe (31) is provided through the middle position inside the sealing plate (30), and the connection position between the feeding pipe (31) and the sealing plate (30) is fixed by resin adhesive.

4. The stem cell screening device according to claim 3, characterized in that: A micro booster pump (32) is provided above the sealing plate (30), and the outer wall surface of the micro booster pump (32) is fixedly assembled with the surface of the sealing plate (30) through a bracket. The discharge end flange of the micro booster pump (32) is butted with a valve (33), and the discharge end of the valve (33) and the feeding pipe (31) are fixed to each other by flanges.

5. The stem cell screening device according to claim 4, characterized in that: Square rods (40) are provided on both sides of the storage seat (13), one end of the square rod (40) is fixed to the surface of the storage seat (13) by screws, and the other end of the square rod (40) is welded with an anti-slip plate (43).

6. The stem cell screening device according to claim 5, characterized in that: The surface of the square rod (40) is slidably sleeved with a square sleeve (41), the outer wall surface of the square sleeve (41) is fixedly sleeved with a clamping seat (42), and the inner side of the clamping seat (42) is covered with the end of the container (14).

7. The stem cell screening device according to claim 6, characterized in that: A second spring (44) is wound around the surface of the square rod (40), and two ends of the second spring (44) are fixedly assembled with the surface of the clamping seat (42) and the surface of the anti-slip plate (43) respectively.

8. The stem cell screening device according to claim 1, characterized in that: The front of the housing (10) is provided with a doorway, the port of the doorway is covered with a door panel (50), the lower end of the door panel (50) is rotatably mounted on the front of the housing (10) via a door hinge, the upper end of the door panel (50) is opened and closed with the doorway position of the housing (10) via a door lock, and a handle (51) is fixed on the outward side of the door panel (50) by screws.

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