Cell separation and extraction device

Abstract

The utility model provides a cell separation and extraction device which comprises a magnet unit, the magnet unit comprises a first magnet assembly and a second magnet assembly, the first magnet assembly and the second magnet assembly are oppositely arranged in a spaced mode in the left-right direction of the cell separation and extraction device, and the first magnet assembly at least comprises a first magnet block, a second magnet block and a third magnet block; the first magnetic block, the second magnetic block and the third magnetic block are linearly and sequentially arranged in the front-back direction of the cell separation and extraction device, the first magnetic block, the second magnetic block and the third magnetic block form a Halbach array, the magnetic induction line direction in the second magnetic block points to the second magnet assembly, and the second magnet assembly at least comprises a fourth magnetic block, a fifth magnetic block and a sixth magnetic block; the fourth magnetic block, the fifth magnetic block and the sixth magnetic block are linearly and sequentially arranged in the front-back direction of the cell separation and extraction device, the fourth magnetic block, the fifth magnetic block and the sixth magnetic block form a Halbach array, and the direction of a magnetic induction line in the fifth magnetic block is the same as that of a magnetic induction line in the second magnetic block.

Description

Technical Field

[0001] The present application belongs to the field of cell therapy technology, and in particular relates to a cell separation and extraction device. Background Art

[0002] Currently, the magnetic field intensity in the working area of ​​the cell separation and extraction device is low, resulting in a low recovery rate of magnetic beads in the sorting column and causing waste. Utility Model Content

[0003] The present application aims to propose a cell separation and extraction device that increases the magnetic field intensity in the working area.

[0004] The embodiment of the present application provides a cell separation and extraction device for applying magnetic force to a sorting column, characterized in that it includes a magnet unit, wherein the magnet unit includes a first magnet assembly and a second magnet assembly.

[0005] The first magnet assembly and the second magnet assembly are arranged opposite to each other in a left-right direction of the cell separation and extraction device, and a magnetic field is formed between the first magnet assembly and the second magnet assembly.

[0006] The first magnet assembly includes at least a first magnetic block, a second magnetic block, and a third magnetic block. The first magnetic block, the second magnetic block, and the third magnetic block are arranged in a straight line in the front-to-back direction of the cell separation and extraction device. The first magnetic block, the second magnetic block, and the third magnetic block constitute a Halbach array. The direction of the magnetic flux lines inside the second magnetic block points to the second magnet assembly.

[0007] The second magnet assembly includes at least a fourth magnetic block, a fifth magnetic block and a sixth magnetic block, and the fourth magnetic block, the fifth magnetic block and the sixth magnetic block are arranged in a straight line in the front-to-back direction of the cell separation and extraction device. The fourth magnetic block, the fifth magnetic block and the sixth magnetic block constitute a Halbach array, and the direction of the magnetic lines of force inside the fifth magnetic block is the same as the direction of the magnetic lines of force inside the second magnetic block.

[0008] In at least one possible embodiment, when viewed from the top and bottom of the cell separation and extraction device, the magnet unit is U-shaped as a whole.

[0009] The magnetic flux lines inside the first magnetic block point to the second magnetic block, and the magnetic flux lines inside the third magnetic block point to the second magnetic block. The first magnetic block is close to the opening of the U shape, and the third magnetic block is close to the bottom of the U shape.

[0010] In at least one possible implementation, in the front-to-back direction, the length of the second magnetic block is greater than the length of the first magnetic block, and the length of the second magnetic block is greater than the length of the third magnetic block.

[0011] In at least one possible embodiment, when observed along the up and down directions of the cell separation and extraction device, the magnet unit is U-shaped as a whole, the direction of the magnetic lines of force inside the fourth magnetic block points away from the fifth magnetic block, the direction of the magnetic lines of force inside the sixth magnetic block points away from the fifth magnetic block, the fourth magnetic block is close to the opening of the U-shape, and the sixth magnetic block is close to the bottom of the U-shape.

[0012] In at least one possible implementation, in the front-to-back direction, the length of the fifth magnetic block is greater than the length of the fourth magnetic block, and the length of the fifth magnetic block is greater than the length of the sixth magnetic block.

[0013] In at least one possible implementation manner, the fourth magnetic block and the first magnetic block are arranged opposite to each other, the fifth magnetic block and the second magnetic block are arranged opposite to each other, and the sixth magnetic block and the third magnetic block are arranged opposite to each other.

[0014] In at least one possible embodiment, the magnet unit also includes a shielding shell. When viewed in the up and down directions of the cell separation and extraction device, the shielding shell is U-shaped as a whole. The first magnet assembly and the second magnet assembly are installed on the two arms of the U-shape of the shielding shell, and the shielding shell is made of soft magnetic material.

[0015] In at least one possible implementation manner, the shielding shell is provided with two mounting grooves, the two mounting grooves respectively accommodating the first magnet assembly and the second magnet assembly, and fixing plates are installed at openings of the two mounting grooves.

[0016] In at least one possible embodiment, the cell separation and extraction device further includes a housing, and the magnet unit is connected to the housing so as to be slidable along the front-to-back direction relative to the housing.

[0017] In at least one possible embodiment, the cell separation and extraction device further includes a linear drive mechanism, which includes a motor, a screw rod, a guide rail, and a slider.

[0018] The screw rod is connected to the output shaft of the motor, the slider and the screw rod are threaded together, the guide rail extends along the front and rear directions of the cell separation and extraction device, the slider can be slidably installed on the guide rail along the guide rail, and the magnet unit is connected to the slider, so that the motor can drive the magnet unit to move along the front and rear directions of the cell separation and extraction device.

[0019] By adopting the above technical solution, the first magnet assembly and the second magnet assembly use magnetic blocks arranged in a Halbach array, so that fewer magnetic blocks can provide a stronger magnetic field strength, thereby increasing the recovery rate of magnetic beads in the separation column. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic structural diagram of a cell separation and extraction device according to an embodiment of the present application is shown.

[0021] Figure 2 A schematic structural diagram of a cell separation and extraction device according to an embodiment of the present application is shown from another angle.

[0022] Figure 3 A schematic structural diagram of a magnet unit of a cell separation and extraction device according to an embodiment of the present application is shown.

[0023] Figure 4 A schematic structural diagram of a magnet unit (magnet assembly and upper cover plate are not shown) of a cell separation and extraction device according to an embodiment of the present application is shown.

[0024] Figure 5 A schematic structural diagram of a magnet assembly, a shielding shell, and a fixing plate of a cell separation and extraction device according to an embodiment of the present application is shown.

[0025] Figure 6 A schematic diagram of a magnet assembly and magnetic flux lines thereof of a cell separation and extraction device according to an embodiment of the present application is shown.

[0026] Figure 7 A schematic structural diagram of the housing of a cell separation and extraction device according to an embodiment of the present application is shown.

[0027] Figure 8 A schematic structural diagram of the housing of the cell separation and extraction device according to an embodiment of the present application is shown from another angle.

[0028] Description of Reference Numerals

[0029] 1 Shell 11 Support plate mounting hole Cavity 12

[0030] 2 magnet units

[0031] 21 magnet assembly

[0032] 211 first magnet assembly 2111 first magnetic block 2112 second magnetic block 2113 third magnetic block

[0033] 212 second magnetic assembly 2121 fourth magnetic block 2122 fifth magnetic block 2123 sixth magnetic block

[0034] 22 shielding shell 23 upper cover 24 lower cover 25 fixing plate

[0035] 3 linear drive mechanism 31 motor 32 screw 33 guide rail 34 slider

[0036] X is the front-back direction, Y is the left-right direction, and Z is the up-down direction. DETAILED DESCRIPTION

[0037] In order to more clearly illustrate the above-mentioned purposes, features and advantages of the present application, this section describes in detail the specific embodiments of the present application in conjunction with the accompanying drawings. In addition to the various embodiments described in this section, the present application can also be implemented in other different ways. Without violating the spirit of the present application, those skilled in the art can make corresponding improvements, deformations and substitutions. Therefore, the present application is not limited to the specific embodiments disclosed in this section. The scope of protection of this application shall be based on the claims.

[0038] like Figures 1 to 8 As shown, an embodiment of the present application provides a cell separation and extraction device, which includes a housing 1, a magnet unit 2 and a linear drive mechanism 3. The magnet unit 2 is connected to the housing 1 so as to be slidable along a front-to-rear direction X relative to the housing 1.

[0039] like Figure 1 and Figure 2 As shown, the linear drive mechanism 3 may include a motor 31, a screw 32, a guide rail 33, and a slider 34. The screw 32 is connected to the output shaft of the motor 31, the slider 34 and the screw 32 are threadedly engaged, the guide rail 33 extends along the front-to-back direction X of the cell separation and extraction device, and the slider 34 is slidably mounted on the guide rail 33. The magnet unit 2 is connected to the slider 34 and can be driven by the motor 31 to move along the front-to-back direction X of the cell separation and extraction device.

[0040] like Figures 6 to 8 As shown, the housing 1 may be provided with a support plate mounting hole 11, to which a sorting column support plate may be connected. The sorting column support plate is used to support and place the sorting column 100 so that the sorting column 100 is in the working area.

[0041] The housing 1 is provided with a cavity 12 which is capable of at least partially accommodating the magnet unit 2 (see Figure 2 When the magnet unit 2 is placed in the housing 1, the sorting column 100 can be in the magnetic field of the magnet unit 2, thereby attracting the magnetic beads in the sorting column 100. When the magnet unit 2 is removed from the housing 1, the sorting column 100 leaves the magnetic field, and the magnetic beads in the sorting column 100 can be eluted.

[0042] like Figures 3 to 6 As shown, the magnet unit 2 includes a magnet assembly 21 , a shielding shell 22 , an upper cover plate 23 , a lower cover plate 24 and a fixing plate 25 .

[0043] like Figure 5 and Figure 6As shown, the magnet assembly 21 may include a first magnet assembly 211 and a second magnet assembly 212. The first magnet assembly 211 and the second magnet assembly 212 may each be a rectangular block. The first magnet assembly 211 and the second magnet assembly 212 may be spaced apart and arranged opposite to each other along the left-right direction Y. A sorting column is placed between the first magnet assembly 211 and the second magnet assembly 212 so that the sorting column is within the magnetic field of the magnet assembly 21. When viewed along the vertical direction Z, the magnet unit 2 may be U-shaped as a whole, with the first magnet assembly 211 and the second magnet assembly 212 respectively located at the two arms of the U-shape.

[0044] When viewed along the up-down direction Z, the shielding shell 22 can be U-shaped as a whole. The shielding shell 22 can have two oppositely arranged mounting grooves 221, and the two mounting grooves 221 are located at two opposite arms of the U-shape. The first magnet assembly 211 and the second magnet assembly 212 are respectively arranged in the two mounting grooves 221. The upper cover 23 and the lower cover 24 are respectively connected to the two ends of the shielding shell 22 in the up-down direction Z, and the two fixing plates 25 are respectively connected to the openings of the two mounting grooves 221. The fixing plates 25 can be made of non-magnetic material. For example, the fixing plates 25 can be made of 1 mm thick stainless steel plates. This does not affect the magnetic field strength, and has sufficient mechanical strength to fix the magnet assembly 21 without causing deformation of the magnet unit 2. The upper cover 23 and the lower cover 24 can be made of non-magnetic materials such as stainless steel, plastic, aluminum alloy, etc. Optionally, the upper cover 23 and the lower cover 24 can be made of the same material as the fixing plate 25.

[0045] The shielding shell 22 can be made of a soft magnetic material such as iron. The shielding shell 22 can be magnetized to form a magnetic circuit, thereby enhancing the magnetic field strength in the working area (i.e., between the first magnet assembly 211 and the second magnet assembly 212). The shielding shell 22 can also shield the magnetic field, reduce magnetic leakage, and maintain a low magnetic field strength in the non-working area, for example, less than or equal to 50 gauss.

[0046] like Figure 5 As shown, the first magnet assembly 211 includes at least a first magnetic block 2111, a second magnetic block 2112, and a third magnetic block 2113. The first magnetic block 2111, the second magnetic block 2112, and the third magnetic block 2113 can be arranged linearly along the front-to-back direction X of the cell separation and extraction device, with the first magnetic block 2111 and the third magnetic block 2113 respectively disposed on either side of the second magnetic block 2112. The first magnetic block 2111 can be located near the opening of the U-shape, and the third magnetic block 2113 can be located near the bottom of the U-shape. The first magnetic block 2111, the second magnetic block 2112, and the third magnetic block 2113 form a Halbach array.

[0047] The direction of the magnetic flux lines inside the first magnetic block 2111 can point to the second magnetic block 2112 , the direction of the magnetic flux lines inside the second magnetic block 2112 can point to the second magnet assembly 212 , and the direction of the magnetic flux lines inside the third magnetic block 2113 can point to the second magnetic block 2112 .

[0048] The second magnet assembly 212 includes at least a fourth magnetic block 2121, a fifth magnetic block 2122, and a sixth magnetic block 2123. The fourth magnetic block 2121, the fifth magnetic block 2122, and the sixth magnetic block 2123 can be arranged linearly along the front-to-back direction X of the cell separation and extraction device. The fourth magnetic block 2121 and the sixth magnetic block 2123 are respectively positioned on either side of the fifth magnetic block 2122. The fourth magnetic block 2121 can be positioned near the opening of the U-shaped structure, and the sixth magnetic block 2123 can be positioned near the bottom of the U-shaped structure. The fourth magnetic block 2121, the fifth magnetic block 2122, and the sixth magnetic block 2123 form a Halbach array.

[0049] The direction of the magnetic flux lines within the fourth magnetic block 2121 can point away from the fifth magnetic block 2122, and the fourth magnetic block 2121 and the first magnetic block 2111 can be arranged opposite each other. The direction of the magnetic flux lines within the fifth magnetic block 2122 is the same as the direction of the magnetic flux lines within the second magnetic block 2112, and the fifth magnetic block 2122 and the second magnetic block 2112 can be arranged opposite each other. The direction of the magnetic flux lines within the sixth magnetic block 2123 can point away from the fifth magnetic block 2122, and the sixth magnetic block 2123 and the third magnetic block 2113 can be arranged opposite each other.

[0050] The magnetic circuit formed by the first magnet assembly 211 and the second magnet assembly 212 arranged in this manner can generate a stronger magnetic field in the working area using fewer magnetic blocks, thereby improving the recovery rate of magnetic beads in cell sorting by the sorting column 100. For example, the magnetic field strength in the working area can be greater than 1 Tesla (i.e., 10,000 gauss).

[0051] Furthermore, in the front-to-back direction X, the length of the second magnetic block 2112 may be greater than the length of the first magnetic block 2111 , the length of the second magnetic block 2112 may be greater than the length of the third magnetic block 2113 , and the length of the first magnetic block 2111 may be equal to the length of the third magnetic block 2113 .

[0052] Furthermore, in the front-to-back direction X, the length of the fifth magnetic block 2122 may be greater than the length of the fourth magnetic block 2121 , the length of the fifth magnetic block 2122 may be greater than the length of the sixth magnetic block 2123 , and the length of the fourth magnetic block 2121 may be equal to the length of the sixth magnetic block 2123 .

[0053] It should be understood that at least some aspects or features of the above-mentioned embodiments, examples or examples may be appropriately combined.

[0054] It is understood that in this application, when the number of parts or components is not specifically limited, the number may be one or more, and the term "plurality" herein refers to two or more. Where the number of parts or components is shown in the drawings and / or described in the specification as a specific number, such as two, three, or four, the specific number is generally illustrative and not restrictive, and may be understood as a plurality, i.e., two or more. However, this does not mean that this application excludes the case of one.

[0055] In this application, unless otherwise clearly stated or limited, terms such as "install", "assemble", "connect", "connect", "couple", "link", "abut", "connect", "interconnect", "communicate", "conduct", "fix", "fasten", etc. should be understood in a broad sense, for example, they can be direct or indirect. For example, with respect to connection, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly stated or limited. For example, with respect to connectivity / conduction, it can be direct connectivity / conduction or indirect connectivity / conduction through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0056] In the present application, unless otherwise clearly stated or limited, a component provided on / installed on / located on / accommodated on / placed in, within, inside, etc. another component may be any of the following two situations: a part or most of the one component is located in the other component; and the one component is completely accommodated in the other component.

[0057] While the present application has been described in detail using the above-described embodiments, it will be apparent to those skilled in the art that the present application is not limited to the embodiments described in this specification. The present application can be modified and implemented as modified embodiments without departing from the subject matter and scope of the present application as defined by the claims. Therefore, the descriptions in this specification are for illustrative purposes only and do not have any limiting meaning with respect to the present application.

Claims

1. A cell separation and extraction device for applying magnetic force to a separation column, characterized in that: comprising a magnet unit, the magnet unit comprising a first magnet assembly and a second magnet assembly, The first magnet assembly and the second magnet assembly are arranged opposite to each other in a left-right direction of the cell separation and extraction device, and a magnetic field is formed between the first magnet assembly and the second magnet assembly. The first magnet assembly includes at least a first magnetic block, a second magnetic block, and a third magnetic block. The first magnetic block, the second magnetic block, and the third magnetic block are arranged in a straight line in the front-to-back direction of the cell separation and extraction device. The first magnetic block, the second magnetic block, and the third magnetic block constitute a Halbach array. The direction of the magnetic flux lines inside the second magnetic block points to the second magnet assembly. The second magnet assembly includes at least a fourth magnetic block, a fifth magnetic block and a sixth magnetic block, and the fourth magnetic block, the fifth magnetic block and the sixth magnetic block are arranged in a straight line in the front-to-back direction of the cell separation and extraction device. The fourth magnetic block, the fifth magnetic block and the sixth magnetic block constitute a Halbach array, and the direction of the magnetic lines of force inside the fifth magnetic block is the same as the direction of the magnetic lines of force inside the second magnetic block.

2. The cell separation and extraction device according to claim 1, characterized in that: When viewed from the top and bottom of the cell separation and extraction device, the magnet unit is U-shaped as a whole. The magnetic flux lines inside the first magnetic block point to the second magnetic block, and the magnetic flux lines inside the third magnetic block point to the second magnetic block. The first magnetic block is close to the opening of the U shape, and the third magnetic block is close to the bottom of the U shape.

3. The cell separation and extraction device according to claim 2, characterized in that: In the front-to-back direction, the length of the second magnetic block is greater than that of the first magnetic block, and the length of the second magnetic block is greater than that of the third magnetic block.

4. The cell separation and extraction device according to claim 1, characterized in that: Observing along the up and down directions of the cell separation and extraction device, the magnet unit is U-shaped as a whole, the direction of the magnetic flux lines inside the fourth magnetic block points away from the fifth magnetic block, the direction of the magnetic flux lines inside the sixth magnetic block points away from the fifth magnetic block, the fourth magnetic block is close to the opening of the U-shape, and the sixth magnetic block is close to the bottom of the U-shape.

5. The cell separation and extraction device according to claim 1, characterized in that: In the front-to-back direction, the length of the fifth magnetic block is greater than that of the fourth magnetic block, and the length of the fifth magnetic block is greater than that of the sixth magnetic block.

6. The cell separation and extraction device according to claim 1, characterized in that: The fourth magnetic block is arranged opposite to the first magnetic block, the fifth magnetic block is arranged opposite to the second magnetic block, and the sixth magnetic block is arranged opposite to the third magnetic block.

7. The cell separation and extraction device according to claim 1, characterized in that: The magnet unit also includes a shielding shell. When viewed in the up and down direction of the cell separation and extraction device, the shielding shell is U-shaped as a whole. The first magnet assembly and the second magnet assembly are installed on the two arms of the U-shape of the shielding shell. The shielding shell is made of soft magnetic material.

8. The cell separation and extraction device according to claim 7, characterized in that: The shielding shell is provided with two mounting grooves, which respectively accommodate the first magnet assembly and the second magnet assembly. A fixing plate is installed at the openings of the two mounting grooves, and the fixing plate is made of non-magnetic material.

9. The cell separation and extraction device according to claim 7, characterized in that: The cell separation and extraction device further includes a housing, and the magnet unit is connected to the housing so as to be slidable along the front-rear direction relative to the housing.

10. The cell separation and extraction device according to claim 1, characterized in that: The cell separation and extraction device further includes a linear drive mechanism, which includes a motor, a lead screw, a guide rail, and a slider. The screw rod is connected to the output shaft of the motor, the slider and the screw rod are threaded together, the guide rail extends along the front and rear directions of the cell separation and extraction device, the slider can be slidably installed on the guide rail along the guide rail, and the magnet unit is connected to the slider, so that the motor can drive the magnet unit to move along the front and rear directions of the cell separation and extraction device.

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