Top light-transmitting PCR (Polymerase Chain Reaction) tube and PCR tube strip

Abstract

The PCR tube comprises a tube body and a tube cover, the tube cover comprises a hollow cover body and a transparent membrane, the cover body comprises a top cover and a convex plug which are integrally formed, the outer diameter of the top cover is larger than that of the convex plug, the top cover is fixedly arranged at the top end of the convex plug, an opening is formed in the top cover, and the transparent membrane is arranged in the opening. An annular step face is formed between the opening and the top end of the protruding plug, the transparent membrane is fixedly arranged on the step face and seals the opening, the outer diameter of the protruding plug is gradually enlarged in the color direction of the protruding plug, the tube body is in a bullet shape, the inner diameter of the tube body is gradually reduced from the tube opening to the tube bottom of the tube body, the protruding plug is arranged in the tube body in a clamped mode, and the transparent membrane is arranged on the protruding plug. When the top cover abuts against the pipe opening, the outer wall of the protruding plug is tightly attached to the inner wall of the pipe body so as to seal the pipe body. According to the scheme, the transparent diaphragm is fixedly arranged at the top of the cover body to form the top surface of the cover body, so that a fluorescence signal is effectively reflected, and a real-time fluorescence quantitative PCR detection result is optimized.

Description

Technical Field

[0001] The utility model relates to the technical field of PCR tools, in particular to a top-light-transmitting PCR tube and a PCR tube strip. Background Art

[0002] Currently, PCR technology is indispensable in molecular biology research and clinical medical diagnostics. PCR, or polymerase chain reaction (PCR), is a method for rapidly amplifying specific genes or DNA sequences in vitro, hence its name, "in vitro gene amplification." PCR reactions often utilize tools such as PCR tubes, PCR tube strips, and PCR tube plates. Existing PCR tubes, PCR tube strips, and PCR tube plates all include a tube body, which is sealed during storage using a cap inserted into the tube opening. This is exemplified by the PCR tubes disclosed in Publication No. CN205077049U.

[0003] In real-time fluorescence quantitative PCR experiments, fluorescence is measured by adding fluorescent groups to the PCR reaction system. These fluorescent groups emit fluorescent signals during the PCR cycles. By detecting the intensity of these signals, the amount of PCR product can be monitored in real time, allowing for quantitative analysis of the target sequence in the sample. The mathematical principle behind this method involves the Ct value, which is the number of cycles required for the signal intensity to reach a set fluorescence threshold. The fluorescence threshold is typically set at 10 times the standard deviation of the fluorescence signal over the first 15 cycles of the PCR reaction to ensure measurement accuracy. In fluorescence quantitative PCR experiments, the light transmittance of the PCR tube directly affects the accuracy and reliability of the real-time fluorescence quantitative PCR results. Conventional PCR tube lids are injection molded, resulting in a certain thickness. Furthermore, to ensure a tight seal between the lid and the tube, a protruding buckle is positioned in the center of the lid, which fits snugly against the tube. This buckle further increases the thickness of the lid, resulting in incomplete reflection of the fluorescence value from the lid, affecting the accuracy of the fluorescence quantitative PCR results. However, the conventional injection molding process is particularly limited, which means that the middle thickness of the cover formed by injection molding cannot be too low, otherwise it will cause adverse reactions such as glue shortage or weld lines. Utility Model Content

[0004] The purpose of the utility model is to overcome the deficiencies in the prior art and provide a top-light-transmitting PCR tube and a PCR tube strip.

[0005] The purpose of this utility model is achieved through the following technical solutions:

[0006] A top-transparent PCR tube comprises a tube body and a tube cap, wherein the tube cap comprises an internally hollow cap body and a transparent membrane, and the cap body comprises an integrally formed top cap and a convex plug. The top cap has an outer diameter greater than that of the convex plug and is fixedly disposed at the top end of the convex plug. The top cap has an opening therein, and an annular step surface is formed between the opening and the top end of the convex plug. The transparent membrane is fixedly disposed on the step surface and seals the opening. The outer diameter of the convex plug gradually increases in its extension direction. The tube body is bullet-shaped, and its inner diameter gradually decreases from its tube mouth to its tube bottom. The convex plug is clamped in the tube body. When the top cap abuts the tube mouth, the outer wall of the convex plug tightly fits the inner wall of the tube body to seal the tube body.

[0007] Preferably, welding ribs are provided on the step surface, and the transparent membrane is fixed on the step surface by ultrasonic welding.

[0008] Preferably, the top of the transparent film does not protrude beyond the top surface of the top cover.

[0009] Preferably, the transparent film is a high-gloss glass slide.

[0010] Preferably, the thickness of the transparent film is 0.25±0.02 mm.

[0011] Preferably, a convex ring is provided on the outer wall of the bottom end of the convex plug.

[0012] Preferably, one side of the top cover has an outwardly protruding ear portion, forming a water drop shape, and when the cover body is placed on the tube body, the ear portion protrudes outward from the tube body.

[0013] Preferably, the other side of the top cover facing the ear is connected to the edge of the tube mouth of the tube body through a soft connecting arm, and the thickness of the inner wall at both ends of the connecting arm is greater than the thickness of the middle inner wall.

[0014] A PCR tube strip comprises a group of PCR tubes as described above, wherein the tubes in a group are connected to each other by a connecting block, wherein the connecting block comprises a middle portion and connecting sides symmetrically arranged on both sides of the middle portion, wherein the thickness of the connecting sides gradually decreases outward from the middle portion, and the bottom of the connecting side extends obliquely from the middle portion, and each connecting side is fixedly connected to the side of a tube body, so that the tubes in a group are arranged in a straight line.

[0015] Preferably, both sides of the tube openings of each of the tube bodies in a group are connected via an arc-shaped connecting arm, and the arc-shaped connecting arm is integrally formed with the tube body in a group.

[0016] The beneficial effects of the present invention are mainly reflected in:

[0017] 1. This solution replaces the top middle structure of the traditional one-piece injection-molded cover with a transparent film, which is fixed to the top of the cover by ultrasonic welding to form the top surface of the cover. On the one hand, the transparent properties and thin thickness of the transparent film itself can ensure the light transmittance of the top of the cover, so as to ensure that the top of the cover can effectively reflect the fluorescent signal, thereby achieving the purpose of reducing cross-contamination of signals between wells and optimizing the detection results of real-time fluorescence quantitative PCR; on the other hand, the transparent film is tightly connected to the cover by ultrasonic welding, which can effectively ensure the sealing of the cover and the sealing effect on the tube body. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The technical solution of the utility model is further described below with reference to the accompanying drawings:

[0019] Figure 1 : Schematic diagram of the explosion of PCR tube;

[0020] Figure 2 : Cross-sectional view of PCR tube strip;

[0021] Figure 3 : Schematic diagram of PCR tube strips. DETAILED DESCRIPTION

[0022] The present invention will be described in detail below with reference to the specific embodiments shown in the accompanying drawings. However, these embodiments are not limited to the present invention, and any structural, methodological, or functional modifications made by a person skilled in the art based on these embodiments are included within the scope of protection of the present invention.

[0023] In the description of the scheme, it should be noted that the terms "center", "up", "down", "left", "right", "front", "back", "vertical", "horizontal", "inside", "outside", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience and simplification of description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they should not be understood as limitations on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and should not be understood as indicating or implying relative importance. Moreover, in the description of the scheme, with the operator as a reference, the direction close to the operator is the proximal end, and the direction away from the operator is the distal end.

[0024] like Figures 1 to 3As shown, the present invention discloses a top-transparent PCR tube, comprising a tube body 1 and a tube cover 2, wherein the tube cover 2 comprises a hollow cover body and a transparent membrane 202, wherein the cover body comprises an integrally formed top cover 201 and a convex plug 203, wherein the top cover 201 has an outer diameter larger than that of the convex plug 203 and is fixed to the top end of the convex plug 203, wherein the top cover 201 has an opening 2011 in the top cover 201, and an annular ring is formed between the opening 2011 and the top end of the convex plug 203. The transparent film 202 is fixed on the stepped surface 2012 and seals the opening 2011. The outer diameter of the convex plug 203 gradually increases from its extension direction. The tube body 1 is bullet-shaped, and its inner diameter gradually decreases from its tube mouth to its tube bottom. The convex plug 203 is clamped in the tube body 1. When the top cover 201 abuts the tube mouth, the outer wall of the convex plug 203 fits tightly against the inner wall of the tube body 1 to seal the tube body 1.

[0025] This solution replaces the top intermediate structure of the traditional integrally-molded cover with a transparent film 202, forming the top surface of the cover 201. The transparent properties and thinness of the transparent film 202 ensure the light transmittance of the top of the cover, ensuring that the top of the cover can effectively reflect the fluorescent signal, thereby achieving the purpose of reducing cross-contamination of signals between wells and optimizing the detection results of real-time fluorescence quantitative PCR. The transparent film 202 is a high-gloss glass slide, which utilizes its excellent light transmittance to effectively refract the fluorescent signal. In the preferred embodiment, the light transmittance of the transparent film 202 can reach 91%, which is significantly higher than that of the traditional injection-molded cover.

[0026] Specific examples Figure 1 As shown, welding ribs 2013 are provided on the stepped surface 2012, and the transparent film 202 is fixed to the stepped surface 2012 by ultrasonic welding. In a preferred embodiment, the transparent film 202 is tightly connected to the cover body by ultrasonic welding, which can effectively ensure the sealing of the cover body and the sealing effect on the tube body 1.

[0027] Furthermore, the top of the transparent film 202 does not protrude beyond the top surface of the top cover 201 to prevent unnecessary wear of the transparent film 202. The thickness of the transparent film 202 is 0.25±0.02 mm to further ensure high light transmittance.

[0028] In order to further improve the sealing performance of the cover, a convex ring 2031 is provided on the outer wall of the bottom end of the convex plug 203. The convex ring 2031 further increases the outer diameter of the convex plug 203, ensuring the tightness between the outer wall of the convex plug 203 and the inner wall of the tube body 1 when it is clamped in the tube body 1.

[0029] like Figure 1-Figure 3 As shown, one side of the top cover 201 has an outwardly protruding ear portion 2014 in the shape of a teardrop. When the cover is placed on the tube body 1, the ear portion 2014 protrudes from the tube body 1. The outwardly protruding structure of the ear portion 2014 makes it easier for operators to remove or install the tube cover 2.

[0030] The other side of the top cover 201 opposite to the ear portion 2014 is connected to the edge of the tube mouth of the tube body 1 through a soft connecting arm 2015. The inner wall thickness of the two ends of the connecting arm 2015 is greater than the thickness of the inner wall in the middle. Such a structure can ensure that the connecting arm 2015 is effectively connected to the tube cover 2 and the tube body 1, making it difficult for the tube cover 2 to be lost. It also facilitates the folding of the connecting arm 2015 when installing the tube cover 2, avoiding affecting the insertion distance of the convex plug 203, thereby ensuring a sealed connection between the tube cover 2 and the tube body 1.

[0031] Further, such as Figure 2 、 Figure 3 As shown, this solution also discloses a PCR tube strip comprising a group of PCR tubes as described above. Each pair of tubes 1 in a group is connected by a connecting block 3. The connecting block 3 comprises a middle portion 301 and connecting sides 302 symmetrically arranged on either side of the middle portion 301. The thickness of the connecting sides 302 gradually decreases outward from the middle portion 301, and the bottom of the connecting side 302 extends obliquely from the middle portion 301. Each connecting side 302 is fixedly connected to the side of a tube 1, so that the tubes 1 in a group are arranged in a straight line. This structure ensures effective connection between the tubes 1 in a group.

[0032] Furthermore, the two sides of the tube openings of a group of tube bodies 1 are connected by an arc-shaped connecting arm 4 respectively. The arc-shaped connecting arm 4 is integrally formed with a group of tube bodies 1, further enhancing the connection strength of the PCR tube strip and avoiding breakage.

[0033] It should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each implementation method can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

[0034] The series of detailed descriptions listed above are only specific descriptions of feasible implementation methods of the present invention. They are not intended to limit the scope of protection of the present invention. Any equivalent implementation methods or changes that do not deviate from the technical spirit of the present invention should be included in the scope of protection of the present invention.

Claims

1. A top-transparent PCR tube comprising a tube body (1) and a tube cover (2), characterized in that: The tube cover (2) comprises a cover body with a hollow interior and a transparent film (202), wherein the cover body comprises an integrally formed top cover (201) and a convex plug (203), wherein the top cover (201) has an outer diameter greater than that of the convex plug (203) and is fixedly mounted on the top end of the convex plug (203), wherein the top cover (201) has an opening (2011) therein, and an annular step surface (2012) is formed between the opening (2011) and the top end of the convex plug (203), wherein the transparent film ( 202) is fixed on the step surface (2012) and seals the opening (2011), the outer diameter of the convex plug (203) gradually expands from its extension direction, the tube body (1) is bullet-shaped, and its inner diameter gradually decreases from its tube mouth to its tube bottom, the convex plug (203) is clamped in the tube body (1), and when the top cover (201) abuts against the tube mouth, the outer wall of the convex plug (203) is tightly fitted with the inner wall of the tube body (1) to seal the tube body (1).

2. The top-light-transmitting PCR tube according to claim 1, characterized in that: Welding ribs (2013) are provided on the step surface (2012), and the transparent film (202) is fixed on the step surface (2012) by ultrasonic welding.

3. The top-light-transmitting PCR tube according to claim 2, characterized in that: The top of the transparent film (202) does not protrude beyond the top surface of the top cover (201).

4. The top-light-transmitting PCR tube according to claim 3, characterized in that: The transparent film (202) is a high-gloss glass slide.

5. The top-light-transmitting PCR tube according to claim 4, characterized in that: The thickness of the transparent film (202) is 0.25±0.02 mm.

6. The top-light-transmitting PCR tube according to any one of claims 1 to 5, characterized in that: A convex ring (2031) is provided on the outer wall of the bottom end of the convex plug (203).

7. The top-light-transmitting PCR tube according to claim 6, characterized in that: One side of the top cover (201) has an outwardly protruding ear portion (2014) in the shape of a water drop; when the cover is placed on the tube body (1), the ear portion (2014) protrudes outward from the tube body (1).

8. The top-light-transmitting PCR tube according to claim 7, characterized in that: The other side of the top cover (201) facing the ear portion (214) is connected to the edge of the tube mouth of the tube body (1) via a soft connecting arm (215), and the thickness of the inner wall at both ends of the connecting arm (2015) is greater than the thickness of the inner wall in the middle.

9. PCR tube strips, characterized by: The invention comprises a group of PCR tubes as claimed in any one of claims 1 to 8, wherein the tube bodies (1) of the group are connected to each other by a connecting block (3), the connecting block (3) comprises a middle portion (301) and connecting sides (302) symmetrically arranged on both sides of the middle portion (301), the thickness of the connecting sides (302) gradually decreases from the middle portion (301) outwards, and the bottom of the connecting side (302) extends obliquely from the middle portion (301), and each connecting side (302) is fixedly connected to the side of a tube body (1), so that the tube bodies (1) of the group are arranged in a straight line.

10. The PCR tube strip according to claim 9, characterized in that: The two sides of the tube openings of each of the tube bodies (1) are connected via an arc-shaped connecting arm (4), and the arc-shaped connecting arm (4) is integrally formed with the tube body (1).

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