Filtering and liquid changing linkage device for cell culture medium

Abstract

The utility model belongs to the technical field of cell culture, and particularly relates to a cell culture medium filtering and liquid changing linkage device which comprises an incubator, a first filtering layer is slidably connected to the inner wall of the incubator, a second filtering layer is arranged below the first filtering layer, and the outer wall of the second filtering layer is slidably connected to the inner wall of the incubator. A third filter layer is arranged below the second filter layer, the outer wall of the third filter layer is slidably connected to the inner wall of the incubator, and a heating piece is arranged in the incubator. Through the arrangement of the first filter layer, the second filter layer and the third filter layer, impurities with different particle sizes and properties are respectively removed from a culture medium through a multi-layer filtering step, so that step-by-step purification is realized, the heating element is arranged in the culture box, the cell culture medium is maintained in a proper temperature range, and the cell culture efficiency is improved. The growth of cells under a stable condition is facilitated.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field, concretely is a kind of filter and liquid change linkage device of cell culture medium. BACKGROUND

[0002] Cell culture technical field involves various equipment and technical means for in-vitro growth, propagation and experimental research of cells, and is widely used in biomedical, drug development, genetic engineering and biological product production and other aspects. The process of cell culture requires precise control of environmental conditions, including temperature, humidity, gas concentration and nutrient supply, to ensure that cells can grow and differentiate in the most suitable environment. In this process, the culture medium, as the basic material for cell growth, contains various nutrients and growth factors, and its quality and purity are crucial for cell growth. Filtration and timely liquid change of culture medium are essential steps in cell culture process, aimed at removing metabolic waste and impurities, maintaining the cleanliness and nutrient balance of cell growth environment, and ensuring the reliability and reproducibility of experimental results. The filter and liquid change linkage device of cell culture medium refers to a device that can filter and automatically change the liquid of the culture medium at the same time. Its purpose is to ensure that the culture medium remains clean and has appropriate nutrient levels during cell culture through the linkage of filtration and liquid change processes, thereby promoting the healthy growth and reproduction of cells. This device improves the efficiency of filtration and liquid change through automation, is suitable for various experimental scenarios in cell culture, effectively reduces the pollution risk caused by manual intervention, and ensures the continuity of culture and the accuracy of experiments.

[0003] The existing technology has some obvious deficiencies in the filtration and liquid change process of cell culture medium. Traditional filtration and liquid change operations are usually performed manually, lack of automation and integrated design, which not only makes the operation inefficient, but also easily introduces pollution due to human factors, affecting the quality and repeatability of cell culture. Secondly, the existing culture medium processing system is mainly single filtration or liquid change function, and cannot realize the linkage of the two, which leads to the need to frequently replace equipment or containers during the culture process, increasing the complexity of operation and the risk of pollution. Especially in experiments that require frequent liquid change, the complexity of operation is significantly increased, making it difficult to maintain the continuity and stability of the environment. UTILITY MODEL CONTENT

[0004] In view of the deficiencies of the prior art, the utility model provides a filter and liquid change linkage device of cell culture medium, which solves the problem that the existing filtration and liquid change operations usually rely on manual operation, lack of automation and integrated design, which not only makes the operation inefficient, but also easily introduces pollution due to human factors, affecting the quality and repeatability of cell culture.

[0005] To achieve the above object, the utility model provides the following technical scheme: a kind of filter and liquid change linkage device of cell culture medium, including incubator, the inner wall of the incubator is slidably connected with first filter layer, the lower portion of the first filter layer is provided with second filter layer, the outer wall of the second filter layer is slidably connected in the inner wall of incubator, the lower portion of the second filter layer is provided with third filter layer, the outer wall of the third filter layer is slidably connected in the inner wall of incubator, the inside of the incubator is provided with heating piece, the inner wall of the incubator is fixedly connected with intelligent inductor on one side, the upper surface of the incubator is connected with box cover, the upper surface of the box cover is fixedly connected with operation screen.

[0006] As a preferred technical scheme of the utility model, the outer wall of the first filter layer, the second filter layer and the third filter layer is fixedly connected with a handle.

[0007] As a preferred technical scheme of the utility model, the outer wall of the incubator is provided with a liquid change tank on one side, the upper surface of the liquid change tank is fixedly connected with a liquid pump, the input end of the liquid pump is fixedly connected with a liquid inlet pipe, one end of the liquid inlet pipe penetrates through the upper surface of the liquid change tank and extends to the inside, and the output end of the liquid pump is fixedly connected with a liquid outlet pipe.

[0008] As a preferred technical scheme of the utility model, one end of the liquid outlet pipe is fixedly connected with a shunt pipe, the lower surface of the shunt pipe is provided with a plurality of spray heads, the spray heads are arranged at equal distances on the lower surface of the shunt pipe, one side of the upper surface of the liquid change tank is fixedly connected with a liquid supplement pipe, and one end of the liquid supplement pipe penetrates through the upper surface of the liquid change tank and extends to the inside.

[0009] As a preferred technical scheme of the utility model, the material of the first filter layer is a coarse filter membrane, the material of the second filter layer is a microfiltration membrane, and the material of the third filter layer is an ultrafiltration membrane.

[0010] As a preferred technical scheme of the utility model, the lower outer wall of the incubator is fixedly connected with a waste liquid pipe, the outer wall of the waste liquid pipe is connected with a solenoid valve, and one end of the waste liquid pipe is fixedly connected with a waste liquid tank.

[0011] As a preferred technical scheme of the utility model, the operation screen is electrically connected with the heating piece, the intelligent inductor, the liquid pump and the solenoid valve.

[0012] Compared with the prior art, the utility model provides a filter and liquid change linkage device of cell culture medium, which has the following beneficial effects:

[0013] 1. The cell culture medium filtration and liquid exchange linkage device, through the setting of the first filter layer, the second filter layer and the third filter layer, the culture medium is filtered through multiple filtering steps, and different particle sizes and properties of impurities are removed respectively, so that step-by-step purification is realized, and the multi-layer filtering mechanism effectively improves the purity of the culture medium and reduces the accumulation of residues. The inside of the incubator is provided with a heating element to ensure that the cell culture medium is maintained within a suitable temperature range, which is conducive to the growth of cells under stable conditions. In addition, the inner wall of the incubator is provided with an intelligent sensor, which dynamically adjusts the environment by monitoring the temperature and state of the culture medium in real time, so that the culture conditions are always in the best state. The operation screen on the box cover provides a convenient operation interface for the operator, realizes fine control and monitoring of the filtration and liquid exchange process, and enables the filtration and liquid exchange process of the culture medium to be carried out in an automatic and intelligent manner, which significantly improves the efficiency and reduces the pollution risk caused by manual operation, and ensures the continuous optimization and stability of the cell culture environment.

[0014] 2. The cell culture medium filtration and liquid exchange linkage device, by setting the first, second and third filter layers, respectively using coarse filter membrane, microfiltration membrane and ultrafiltration membrane materials, the culture medium is filtered step by step, and different sizes of impurities and particles are removed step by step, so that the culture medium is more pure. The liquid exchange tank is connected with the liquid pump and the liquid outlet pipe, and the liquid pump pushes the culture medium to flow, so that the liquid exchange process is uniform and continuous. The multiple spray heads arranged below the shunt pipe help to realize uniform liquid spraying, so that the fresh culture medium uniformly covers the cultured cells. The liquid supplement pipe ensures that the culture medium is supplemented in time during the liquid exchange process, so that the nutrition is sufficient and stable. In addition, the connection of the waste liquid pipe and the waste liquid tank enables the waste liquid to be quickly discharged during the liquid exchange process, reducing the influence on the cell environment. The whole process is controlled through the operation screen, and the intelligent sensor can monitor the temperature and culture medium state in real time, ensuring the stability of the culture conditions. These innovations optimize the filtration and liquid exchange linkage process, improve the purification level of the culture medium, ensure the continuous supply of nutrition, and significantly improve the stability and efficiency of the cell culture environment. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a three-dimensional structure schematic diagram of the utility model;

[0016] Figure 2 It is a liquid exchange tank structure schematic diagram of the utility model;

[0017] Figure 3 It is an incubator split structure schematic diagram of the utility model;

[0018] Figure 4 It is a waste liquid tank structure schematic diagram of the utility model.

[0019] In the figure: 1, incubator; 2, first filter layer; 3, second filter layer; 4, third filter layer; 5, heating element; 6, intelligent sensor; 7, box cover; 8, operation screen; 9, handle; 10, liquid exchange tank; 11, liquid pump; 12, liquid inlet pipe; 13, liquid outlet pipe; 14, shunt pipe; 15, spray head; 16, liquid supplement pipe; 17, waste liquid pipe; 18, electromagnetic valve; 19, waste liquid tank. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0021] Embodiment one

[0022] Please refer to Figures 1-4 In the embodiment, the filter and liquid exchange linkage device for cell culture medium comprises an incubator 1, the inner wall of the incubator 1 is slidably connected with a first filter layer 2, the lower part of the first filter layer 2 is provided with a second filter layer 3, the outer wall of the second filter layer 3 is slidably connected to the inner wall of the incubator 1, the lower part of the second filter layer 3 is provided with a third filter layer 4, the outer wall of the third filter layer 4 is slidably connected to the inner wall of the incubator 1, the inside of the incubator 1 is provided with a heating element 5, one side of the inner wall of the incubator 1 is fixedly connected with an intelligent sensor 6, the upper surface of the incubator 1 is connected with a box cover 7, and the upper surface of the box cover 7 is fixedly connected with an operation screen 8.

[0023] In the embodiment, the settings of the first filter layer 2, the second filter layer 3 and the third filter layer 4 make the culture medium pass through multiple filtering steps to remove impurities of different particle sizes and properties, so as to realize step-by-step purification. This multi-layer filtering mechanism effectively improves the purity of the culture medium and reduces the accumulation of residues. The inside of the incubator 1 is provided with a heating element 5 to ensure that the cell culture medium is maintained within a suitable temperature range, which is conducive to the growth of cells under stable conditions. In addition, the intelligent sensor 6 is installed on the inner wall of the incubator 1 to dynamically adjust the environment by real-time monitoring of the temperature and state of the culture medium, so as to ensure that the culture conditions are always in the best state. The operation screen 8 on the box cover 7 provides a convenient operation interface for the operator to realize fine control and monitoring of the filtering and liquid exchange process. The filtering and liquid exchange process of the culture medium can be carried out in an automated and intelligent manner, which significantly improves the efficiency and reduces the pollution risk caused by human operation, ensuring the continuous optimization and stability of the cell culture environment.

[0024] Preferably, the outer wall of each of the first filter layer 2, the second filter layer 3 and the third filter layer 4 is fixedly connected with a handle 9.

[0025] The first filter layer 2, the second filter layer 3 and the third filter layer 4 can be conveniently disassembled, cleaned and replaced through the handle 9.

[0026] Preferably, the outer wall of the incubator 1 is provided with a liquid replacement tank 10, the upper surface of the liquid replacement tank 10 is fixedly connected with a liquid pump 11, the input end of the liquid pump 11 is fixedly connected with a liquid inlet pipe 12, one end of the liquid inlet pipe 12 penetrates through the upper surface of the liquid replacement tank 10 and extends to the inside, the output end of the liquid pump 11 is fixedly connected with a liquid outlet pipe 13, one end of the liquid outlet pipe 13 is fixedly connected with a shunt pipe 14, the lower surface of the shunt pipe 14 is provided with a plurality of spray heads 15, the spray heads 15 are arranged at equal distances on the lower surface of the shunt pipe 14, and the upper surface of the liquid replacement tank 10 is fixedly connected with a liquid supplement pipe 16, one end of the liquid supplement pipe 16 penetrates through the upper surface of the liquid replacement tank 10 and extends to the inside.

[0027] The liquid pump 11 drives the culture medium to flow, ensuring uniformity and continuity of the liquid replacement process. The plurality of spray heads 15 arranged below the shunt pipe 14 help to achieve uniform liquid spraying, ensuring that the fresh culture medium uniformly covers the cultured cells. The liquid supplement pipe 16 ensures that the culture medium is supplemented in time during the liquid replacement process, maintaining sufficient and stable nutrition.

[0028] Further, the material of the first filter layer 2 is a coarse filter membrane, the material of the second filter layer 3 is a microfilter membrane, and the material of the third filter layer 4 is an ultrafilter membrane.

[0029] The first filter layer 2, the second filter layer 3 and the third filter layer 4 are arranged in sequence, and coarse filter membrane, microfilter membrane and ultrafilter membrane materials are respectively used to filter the culture medium step by step, gradually purifying from coarse to fine, ensuring that different sizes of impurities and particles are removed, and the culture medium is more pure.

[0030] Preferably, the lower outer wall of the incubator 1 is fixedly connected with a waste liquid pipe 17, the outer wall of the waste liquid pipe 17 is connected with a solenoid valve 18, and one end of the waste liquid pipe 17 is fixedly connected with a waste liquid tank 19.

[0031] The waste liquid is discharged through the waste liquid pipe 17, keeping the culture environment clean.

[0032] Further, the operation screen 8 is electrically connected with the heating element 5, the intelligent sensor 6, the liquid pump 11 and the solenoid valve 18.

[0033] The entire filtration and liquid replacement linkage process is controlled and adjusted by the operation screen 8.

[0034] The working principle and use process of the utility model: working principle, operator realizes the purification and nutrition supplement of culture medium through multistage filtration system and linkage liquid changing system. Firstly, the culture medium in the incubator 1 is filtered step by step through the first filter layer 2 (coarse filter membrane), the second filter layer 3 (micro filter membrane) and the third filter layer 4 (ultra filter membrane), and different size impurities are removed gradually from coarse to fine, guaranteeing the purity of culture medium. The heating part 5 is used to maintain the proper temperature in the incubator 1, and the intelligent inductor 6 carries out real-time monitoring on the temperature, humidity and other parameters of culture medium, and visual display and operation are carried out through the operation screen 8. The liquid pump 11 is connected with the incubator 1 through the liquid pump 11, and the liquid pump 11 extracts fresh culture medium from the liquid changing tank 10 and sprays it uniformly to the cell culture area through the liquid outlet pipe 13 and the spray head 15 on the shunt pipe 14, ensuring the uniformity and effectiveness of liquid changing. At the same time, the waste liquid is discharged into the waste liquid tank 19 through the waste liquid pipe 17, and the discharge process is controlled by the electromagnetic valve 18, realizing the effective treatment of waste liquid.

[0035] The use process, before use, first, the cell culture medium is added to the liquid changing tank 10, and the amount of culture medium in the liquid changing tank 10 is ensured to be sufficient, and whether the filtration system in the incubator 1 is in normal working state is checked, and it is confirmed that the coarse filter membrane, micro filter membrane and ultra filter membrane are intact, the device is started through the operation screen 8, and the required temperature, liquid changing frequency and other monitoring parameters are set. The heating part 5 will heat the incubator 1 according to the set temperature, ensure that the cells are in a suitable growth environment, and the culture medium enters the filtration stage, passes through the first filter layer 2, the second filter layer 3 and the third filter layer 4 in turn, and gradually removes impurities to ensure that the culture medium reaches the best purity. This process is monitored by the intelligent inductor 6 in real time, so as to adjust the state in the filtration process in time, and the liquid pump 11 is started by the operation screen 8, and the fresh culture medium is transported into the incubator 1 through the liquid inlet pipe 12 and the liquid outlet pipe 13, and the spray head 15 on the shunt pipe 14 ensures that the fresh culture medium enters the culture area uniformly. At the same time, the waste liquid is discharged through the waste liquid pipe 17, and the culture environment is kept clean, and in the liquid changing process, the replenishment pipe 16 automatically adds the supplemented culture medium to the liquid changing tank 10 to maintain the supply amount of culture medium. The waste liquid is discharged into the waste liquid tank 19 by the electromagnetic valve 18, and the whole liquid changing and replenishment process is carried out automatically, which minimizes manual intervention, and the whole filtration and liquid changing linkage process is controlled and adjusted by the operation screen 8, and the intelligent inductor 6 provides real-time data to monitor the culture medium state and temperature and other environmental parameters, so as to ensure the stability of the cell culture environment, and after the experiment is finished, the device power is turned off, the waste liquid in the waste liquid tank 19 is cleaned, the filter layer is checked and the incubator 1 is cleaned, so as to ensure the cleanliness and normal operation of the equipment next time.

[0036] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for the equivalent replacement of part of the technical features of the technical solutions recorded in the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application, should be included within the scope of the present application.

Claims

1. A cell culture medium filtration and medium exchange linkage device, comprising an incubator (1), characterized in that: The inner wall of the incubator (1) is slidably connected to a first filter layer (2), a second filter layer (3) is provided below the first filter layer (2), the outer wall of the second filter layer (3) is slidably connected to the inner wall of the incubator (1), a third filter layer (4) is provided below the second filter layer (3), the outer wall of the third filter layer (4) is slidably connected to the inner wall of the incubator (1), a heating element (5) is provided inside the incubator (1), a smart sensor (6) is fixedly connected to one side of the inner wall of the incubator (1), a lid (7) is connected to the upper surface of the incubator (1), and an operation screen (8) is fixedly connected to the upper surface of the lid (7).

2. The cell culture medium filtration and liquid exchange linkage device according to claim 1, characterized in that: The outer walls of the first filter layer (2), the second filter layer (3), and the third filter layer (4) are all fixedly connected with handles (9).

3. The cell culture medium filtration and liquid exchange linkage device according to claim 1, characterized in that: A liquid exchange tank (10) is provided on one side of the outer wall of the incubator (1). A liquid pump (11) is fixedly connected to the upper surface of the liquid exchange tank (10). An inlet pipe (12) is fixedly connected to the input end of the liquid pump (11). One end of the inlet pipe (12) penetrates the upper surface of the liquid exchange tank (10) and extends into the interior. An outlet pipe (13) is fixedly connected to the output end of the liquid pump (11).

4. The cell culture medium filtration and liquid exchange linkage device according to claim 3, characterized in that: One end of the outlet pipe (13) is fixedly connected to a diversion pipe (14). Multiple nozzles (15) are provided on the lower surface of the diversion pipe (14). The nozzles (15) are arranged at equal intervals on the lower surface of the diversion pipe (14). A replenishment pipe (16) is fixedly connected to one side of the upper surface of the liquid exchange tank (10). One end of the replenishment pipe (16) penetrates the upper surface of the liquid exchange tank (10) and extends into the interior.

5. The cell culture medium filtration and liquid exchange linkage device according to claim 1, characterized in that: The first filter layer (2) is made of a coarse filter membrane, the second filter layer (3) is made of a micro filter membrane, and the third filter layer (4) is made of an ultrafiltration membrane.

6. The cell culture medium filtration and liquid exchange linkage device according to claim 1, characterized in that: The lower outer wall of the incubator (1) is fixedly connected to a waste liquid pipe (17), the outer wall of the waste liquid pipe (17) is connected to a solenoid valve (18), and one end of the waste liquid pipe (17) is fixedly connected to a waste liquid tank (19).

7. The cell culture medium filtration and liquid exchange linkage device according to claim 1, characterized in that: The operation panel (8) is electrically connected to the heating element (5), the intelligent sensor (6), the liquid pump (11), and the solenoid valve (18).

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