Cell sieve device for extracting capillary components in adipose tissue

Abstract

The utility model discloses a cell sieve device for extracting capillary components in adipose tissue, and belongs to the technical field of cells. The device comprises: a housing comprising a cylindrical outer shell; a sample inlet is formed in the top of the shell, a sample outlet is formed in the bottom of the shell, and a sample recovery port and a plugging port are formed in the side wall of the shell; the filtering device comprises a circular-ring-shaped filtering frame; a filter screen is mounted in the filter frame, and the filter frame is detachably inserted into the insertion port, so that the filter screen is mounted in the shell. According to the capillary screening device, efficient screening is achieved, the filter screen which is accurately controlled is adopted, different hole diameters can be selected according to requirements, it is ensured that capillary components in a specific size range are efficiently screened out, and the requirements of different experiments are met. The stainless steel shell and the replaceable nylon filter screen are adopted, the durability is high, repeated use is supported, and the experiment cost and resource waste are reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell technical field, concretely relates to a cell screen device for extracting microvessel component in adipose tissue. BACKGROUND

[0002] Microvascular fragments (MVF) are functional natural vascular units isolated from adipose, brain or other tissues, including arteriole, capillary and venule segments. Adipose tissue contains abundant blood vessels, which is the main source of MVF. Compared with single cells, MVF has a complete 3D structure of capillary, can quickly assemble into a new microvascular network, and shows higher vitality and angiogenic capacity than stromal vascular fractions (SVF) in vivo. It is commonly used for studying angiogenesis and applied to tissue engineering and regenerative medicine as an efficient vascularization unit.

[0003] MVF was first isolated from the epididymal fat pad of rats or mice, and Shepherd et al. also isolated MVF with similar characteristics to rat sources from human liposuction fluid. The isolation of MVF includes digestion, filtration and washing steps.

[0004] The existing MVF filtration needs to stack two disposable cell screens for filtration, or directly use two self-made nylon mesh filters, which is low in efficiency; there are more microvascular fragments remaining on the nylon mesh and cell screen after filtration, which affects the subsequent filtration effect. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a device for screening specific size microvessel components, which is designed based on the structure of stainless steel shell and nylon filter screen, and different pore size nylon screens can be freely combined. The device can accurately screen microvessel components of specific size, and ensure the high activity and integrity of cells during the screening process. At the same time, the device has the advantages of simple operation, reusability and low cost, and is suitable for various biomedical research and clinical application scenarios.

[0006] To solve the above technical problems, the utility model provides a cell screen device for extracting microvessel components in adipose tissue, which comprises:

[0007] The shell comprises a cylindrical shell; a sample inlet is arranged at the top of the shell, a sample outlet is arranged at the bottom of the shell, and a sample recovery port and a plug-in port are arranged on the side wall of the shell;

[0008] Filtering device, it includes circular ring filtering frame, filtering screen is installed in the filtering frame, the filtering frame is inserted into the plug-in interface, so that the filtering screen is installed in the shell.

[0009] Preferably, the number of plug-in interfaces and filtering frames is two.

[0010] Two plug-in interfaces are located at the position close to the top and the position close to the bottom of the shell respectively.

[0011] Preferably, the sample recovery port is located between the two plug-in interfaces.

[0012] Preferably, the flushing port is arranged on the side wall of the shell.

[0013] The flushing port is located between the two plug-in interfaces.

[0014] The flushing port is arranged obliquely downward and faces the filtering screen of the lower filtering frame.

[0015] Preferably, the aperture of the filtering screen of the upper filtering frame is larger than the aperture of the filtering screen of the lower filtering frame.

[0016] Preferably, the apertures of the filtering screens of the two filtering frames are 500 mu m and 30 mu m respectively.

[0017] Preferably, the plug-in interface is a semicircular notch.

[0018] The shell is provided with a corresponding semicircular insertion slot on the inner side wall of the side opposite to the plug-in interface.

[0019] The filtering frame is inserted into the insertion slot after passing through the plug-in interface.

[0020] Preferably, the width of the insertion slot is smaller than the width of the plug-in interface.

[0021] Preferably, the edge of the filtering frame is provided with a semicircular ring-shaped clamping piece protruding upward on the top.

[0022] The clamping piece is the same as and matched with the width of the plug-in interface, and the clamping piece is embedded in the plug-in interface.

[0023] Preferably, the shell is a stainless steel shell.

[0024] The filtering screen is a nylon filtering screen.

[0025] Compared with the prior art, the filtering device has the beneficial effects that:

[0026] 1. Efficient screening: the device adopts a nylon filtering screen with accurate control, can select different apertures according to requirements, and ensures that the microvessel components in a specific size range are efficiently screened out, meeting the requirements of different experiments.

[0027] 2. Cell activity preservation: By the reasonable matching of stainless steel and nylon materials, the device effectively reduces physical damage to cells during screening, ensuring high activity and integrity of microvascular components.

[0028] 3. Simple operation: Sample introduction and flushing processes can be performed through standard syringes, making the operation simple, convenient, and suitable for various laboratory operators.

[0029] 4. Reusable: The device uses a stainless steel shell and replaceable nylon filter screen, which is durable and supports multiple uses, reducing experimental costs and resource waste.

[0030] 5. Wide application: The device is suitable for microvascular screening, cell research, tissue engineering, vascular disease research, and other fields, with wide application prospects. BRIEF DESCRIPTION OF DRAWINGS

[0031] The specific embodiments of the present application will be further described in detail below with reference to the accompanying drawings.

[0032] Figure 1 is a structural schematic diagram of a cell screening device for extracting microvascular components in adipose tissue according to the present application.

[0033] In the drawings:

[0034] 1 - shell; 11 - sample introduction port; 12 - sample outlet; 13 - sample recovery port; 14 - plug-in port; 141 - plug-in slot; 15 - flushing port; 2 - filter frame; 21 - clamping piece; 3 - filter screen. DETAILED DESCRIPTION

[0035] In the following description, many specific details are set forth in order to provide a thorough understanding of the present application. However, the present application can be practiced in a variety of ways beyond the specific embodiments described and should not be limited to these specific embodiments. It can be readily appreciated by those skilled in the art that the present application is not limited to the implementations described and / or illustrated herein and can be implemented in various ways.

[0036] The terms used in one or more embodiments of the present specification are for the purpose of describing specific embodiments only, and are not intended to limit the one or more embodiments of the present specification. The singular forms "a," "an," and "the" used in the one or more embodiments of the present specification and the appended claims are also intended to include the plural forms, unless the context clearly indicates otherwise. It should also be understood that the term "and / or" used in the one or more embodiments of the present specification means and includes any or all possible combinations of one or more associated listed items.

[0037] It should be understood that, although the terms first, second, etc. can be employed in describing various information in one or more embodiments of the present specification, the information should not be limited to such terms. These terms are only used to distinguish one type of information from another type of information. For example, without departing from the scope of one or more embodiments of the present specification, first can also be referred to as second, and similarly, second can also be referred to as first. Depending on the context, the word "if" as used herein can be interpreted as "when" or "upon" or "in response to determining".

[0038] The utility model is further described in detail below in combination with the drawings:

[0039] The utility model provides a cell screen device for extracting microvessel component in fat tissue, include:

[0040] The shell includes a cylindrical shell 1; The shell 1 top is provided with sample introduction port 11, the shell 1 bottom is provided with sample outlet 12, the shell 1 side wall is provided with sample recovery port 13 and plug interface 14;

[0041] The filter device includes a circular ring filter frame 2; The filter frame 2 is installed with filter screen 3, the filter frame 2 is detachably inserted into plug interface 14, thereby installs filter screen 3 in shell 1.

[0042] Preferably, the number of plug interface 14 and filter frame 2 is two;

[0043] Two plug interfaces 14 are located at the position close to the top and the position close to the bottom of shell 1 respectively.

[0044] Preferably, the sample recovery port 13 is located between the two plug interfaces 14.

[0045] Preferably, the shell 1 side wall is provided with flushing port 15;

[0046] The flushing port 15 is located between the two plug interfaces 14;

[0047] The flushing port 15 is arranged obliquely downward, and is directly opposite the filter screen 3 of the lower filter frame 2.

[0048] Preferably, the pore size of the filter screen 3 of the upper filter frame 2 is greater than the pore size of the filter screen 3 of the lower filter frame 2.

[0049] Preferably, the pore sizes of the filter screens 3 of the two filter frames 2 are 500 μm and 30 μm respectively.

[0050] Preferably, the plug interface 14 is a semicircular notch;

[0051] The shell 1 is provided with a corresponding semicircular slot 141 on the inner side wall opposite to the insertion port 14;

[0052] The filter frame 2 is inserted into the slot 141 after passing through the insertion port 14.

[0053] Preferably, the width of the slot 141 is smaller than the width of the insertion port 14.

[0054] Preferably, the filter frame 2 is provided with a semicircular ring-shaped clamping piece 21 protruding upward at the top edge;

[0055] The clamping piece 21 is the same as and matched with the width of the insertion port 14, and is embedded in the insertion port 14.

[0056] Preferably, the shell 1 is a stainless steel shell.

[0057] The filter screen 3 is a nylon filter screen.

[0058] In order to better illustrate the technical effect of the present application, the following specific embodiment is provided to illustrate the above technical process:

[0059] Embodiment 1, a device for screening microvessel components, mainly comprising the following parts:

[0060] 1. Stainless steel shell: the device adopts a stainless steel shell 1, which is designed in a cylindrical or flat shape to ensure the stability and corrosion resistance of the structure. A detachable stainless steel bracket is provided for fixing the nylon filter screen.

[0061] 2. Nylon filter screen: the filter screen 3 is made of nylon material with strong biocompatibility, which has flexibility and durability. According to the experimental requirements, the pore size of the filter screen 3 can be adjusted to screen microvessel components of different size ranges.

[0062] The pore size of the upper filter screen 3 is 500μm, which is used to remove tissue fragments and large vessel fragments, and the pore size of the lower filter screen 3 is 30μm, which is used to filter single cells; in this way, the microvessel fragments with a length of 50-200um are retained on the lower filter screen 3.

[0063] 3. Sample introduction and outlet: the device is provided with a sample introduction port 11 at the top, which can be equipped with a Luer lock port, suitable for standard syringes or other fluid delivery devices. The filtered sample is discharged from the sample outlet 12 at the bottom, which can also use a Luer lock port to ensure the sealing and safety of the connection.

[0064] 4. Syringe flushing device: to avoid the microvessel components on the surface of the filter screen 3 retention, the device side is designed with an additional flushing port 15, connected to the syringe for flushing. By injection buffer or medium, flushing the microvessel components attached to the lower filter screen 3, to ensure the recovery rate and cell activity.

[0065] 5. Modular design: the device uses a modular structure, filter screen 3 can be replaced according to different experimental requirements, the whole device is also easy to disassemble and clean and reuse.

[0066] The utility model discloses a can to filter screen 3 flushing, the utility model discloses also be applicable to all between obtaining a size range within the tissue fragment, not only limit to be used for extracting microvessel fragment.

[0067] The above, only for the specific implementation of the utility model, but the protection scope of the utility model is not limited to this, any change or replacement in the technical range disclosed in the utility model, should be covered in the protection scope of the utility model. Therefore, the protection scope of the utility model should be limited to the protection scope of the said claim.

Claims

1. A cell sieve device for extracting microvascular components from adipose tissue, characterized in that, include: The housing includes a cylindrical outer shell (1); the top of the outer shell (1) is provided with a sample inlet (11), the bottom of the outer shell (1) is provided with a sample outlet (12), and the side wall of the outer shell (1) is provided with a sample retrieval port (13) and an insertion port (14); A filtration device comprising an annular filter frame (2); a filter screen (3) is installed in the filter frame (2), and the filter frame (2) is detachably inserted into a connector (14) to install the filter screen (3) inside a housing (1).

2. The cell sieve device for extracting microvascular components from adipose tissue according to claim 1, characterized in that: The number of the insertion interface (14) and the filter frame (2) are both two; The two connectors (14) are located on the outer casing (1) near the top and near the bottom, respectively.

3. The cell sieve device for extracting microvascular components from adipose tissue according to claim 2, characterized in that: The sample recovery port (13) is located between the two insertion ports (14).

4. The cell sieve device for extracting microvascular components from adipose tissue according to claim 3, characterized in that: A rinsing port (15) is provided on the side wall of the outer casing (1); The flushing port (15) is located between the two insertion ports (14); The rinsing port (15) is set at an angle downwards, directly facing the filter screen (3) of the filter frame (2) below.

5. The cell sieve device for extracting microvascular components from adipose tissue according to claim 4, characterized in that: The aperture of the filter screen (3) of the filter frame (2) above is larger than the aperture of the filter screen (3) of the filter frame (2) below.

6. The cell sieve device for extracting microvascular components from adipose tissue according to claim 5, characterized in that: The filter screens (3) of the two filter holders (2) have apertures of 500 μm and 30 μm, respectively.

7. The cell sieve device for extracting microvascular components from adipose tissue according to claim 6, characterized in that: The insertion interface (14) is a semi-circular notch; The outer casing (1) has a corresponding semi-circular slot (141) on the inner side wall opposite to the insertion interface (14); The filter holder (2) is inserted into the slot (141) after passing through the insertion interface (14).

8. The cell sieve device for extracting microvascular components from adipose tissue according to claim 7, characterized in that: The width of the slot (141) is smaller than the width of the connector (14).

9. The cell sieve device for extracting microvascular components from adipose tissue according to claim 8, characterized in that: The filter frame (2) has an upwardly protruding semi-circular clip (21) at the top edge; The card (21) has the same width as and is compatible with the insertion interface (14), and the card (21) is embedded in the insertion interface (14).

10. The cell sieve device for extracting microvascular components from adipose tissue according to claim 9, characterized in that: The outer casing (1) is a stainless steel casing; The filter screen (3) is a nylon filter screen.

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