Refrigeration cycle device of full-automatic ultralow-temperature storage equipment

Abstract

The utility model discloses a refrigeration cycle device of full-automatic ultralow-temperature storage equipment, the ultralow-temperature storage equipment comprises a storage cabin and a transfer cabin, a transfer channel capable of being controlled to be opened and closed is arranged between the storage cabin and the transfer cabin, the refrigeration cycle device comprises a first refrigeration system, and the first refrigeration system comprises a refrigeration pipeline and a circulation fan blade. Samples are irradiated and refrigerated through the refrigeration pipelines arranged in a contrast mode, cold energy is evenly diffused through the circulating fan blades, and the biological samples are prevented from being stored in a high-pressure environment for a long time in cooperation with the main refrigeration mode of the compressor, the auxiliary refrigeration mode of liquid nitrogen, the evenly-distributed coil pipes, the circulating fan blades and the like, and the biological samples can be stored at normal pressure; the stability, safety and maintainability of equipment and sample storage can be greatly improved, the influence of pressure change on the temperature of the storage cabin is avoided, and the temperature of the storage cabin is in a relatively stable state.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of ultralow temperature biological storage equipment, and especially to a refrigeration circulating device of full-automatic ultralow temperature storage equipment. BACKGROUND

[0002] The ultralow temperature storage equipment is used for storing biological samples, such as: standardized collection, processing, storage and application of biological macromolecules, cells, tissues and organs of health and disease organisms, including human organ tissues, whole blood, plasma, serum, biological fluids or processed biological samples, such as: DNA, RNA, protein biological samples, etc.

[0003] The ultralow temperature storage equipment in the prior art, such as the modularized detachable automatic refrigerator and sample box sample tube in-out warehouse method disclosed by CN114413550B, comprises a vacuum heat insulation storage tank body, the vacuum heat insulation storage tank body is provided with a refrigeration system, and further comprises a detachable frame, a dewar bottle connection mechanism, a sealing isolation mechanism, a grabbing mechanism, a tank opening mechanism and a extraction mechanism. The ultralow temperature storage equipment has the following defects:

[0004] Firstly, the vacuum heat insulation storage tank body adopts a dewar tank form in actual application, and the overall volume and weight of the tank body are large, which is inconvenient for transportation. Moreover, the vacuum tank body in the form of the dewar tank usually adopts a bottom liquid nitrogen filling and liquid nitrogen evaporation mode for refrigeration. The gas phase storage of natural evaporation requires a large space to be reserved between the bottom and the top of the storage tank body, which will result in an excessively large height of the storage tank body. In addition, as the liquid nitrogen evaporates, the pressure inside the tank body will gradually increase. At this time, the biological samples are in a high pressure environment, which is not conducive to the stability and safety of the biological sample storage. The conventional method will set a pressure protection device to relieve the pressure of the storage tank body, that is, the storage tank body is always in a natural pressure increasing and protection pressure relief cycle, and the pressure is in a state of constant change. Due to the correlation between temperature and pressure, the change of pressure will also affect the temperature inside the tank body, which will result in that the temperature inside the storage tank body cannot be kept stable, and it is not conducive to the control of the storage temperature of the biological samples. At the same time, the bottom liquid nitrogen filling and high pressure environment make it difficult to repair the dewar tank when it fails or is damaged, and it is also basically impossible to safely take out the biological samples inside, which brings irreparable loss to the user.

[0005] Secondly, the equipment uses the traditional liquid nitrogen evaporation refrigeration mode. This mode will produce high pressure in the storage cabin due to the evaporation of liquid nitrogen, and the biological samples will be stored in a high pressure environment for a long time, which will affect the stability and safety of the storage. SUMMARY

[0006] The utility model discloses a refrigeration circulating device of full -automatic ultralow temperature storage equipment, the utility model discloses a small, low -cost advantage has can realize normal pressure storage biological sample and accurate thermostatic control to have the maintainability of not influencing sample storage, can improve the stability, security and maintainability of equipment greatly.

[0007] The technical scheme of the utility model is as follows:

[0008] A refrigeration circulating device of full -automatic ultralow temperature storage equipment, the utility model discloses a small, low -cost advantage has can realize normal pressure storage biological sample and accurate thermostatic control to have the maintainability of not influencing sample storage, can improve the stability, security and maintainability of equipment greatly.

[0009] A refrigeration circulating device of full -automatic ultralow temperature storage equipment, the utility model discloses a small, low -cost advantage has can realize normal pressure storage biological sample and accurate thermostatic control to have the maintainability of not influencing sample storage, can improve the stability, security and maintainability of equipment greatly.

[0010] The refrigeration circulating device includes the first refrigeration system of heat exchange with the storage cabin, and the first refrigeration system includes a refrigeration pipeline and a circulating fan blade, the refrigeration pipeline is arranged on the outside of the rotating cage, and is used for radiating refrigeration to the rotating cage, and the circulating fan blade is arranged in the middle of the rotating cage.

[0011] The refrigeration pipeline is coiled and arranged on the outside of the rotating cage support, and a gap for the biological sample box to enter and exit is reserved at the position corresponding to the transfer channel.

[0012] The refrigeration pipeline includes at least one group of refrigeration coils, and the two refrigeration coils of the same group are oppositely arranged on the two sides of the rotating cage support.

[0013] The refrigeration circulating device further includes a second refrigeration system for heat exchange with the transfer cabin, a third refrigeration system for heat exchange with the cold trap, a first temperature monitoring module connected with the first refrigeration system, a second temperature monitoring module connected with the second refrigeration system, and a third temperature monitoring module connected with the third refrigeration system.

[0014] The first temperature monitoring module includes a plurality of first temperature sensors arranged in the inside of the storage cabin, the second temperature monitoring module includes a plurality of second temperature sensors arranged in the inside of the transfer cabin, and the third temperature monitoring module includes a plurality of third temperature sensors arranged in the inside of the cold trap.

[0015] Part of the first temperature sensor is distributed on the prismatic centripetal side of the rotating cage support, the rest of the first temperature sensor is distributed on the side wall of the rotating door facing the storage cabin, and the interval between the upper and lower adjacent first temperature sensors is consistent with the height of the storage groove; the second temperature sensor array is distributed on the workbench top; and the third temperature sensor is distributed on the inner wall of the cold trap.

[0016] The refrigeration cycle device further comprises a cold energy recovery circuit, one end of the cold energy recovery circuit is connected with the liquid nitrogen refrigeration circuit return end through an electromagnetic valve, and at least part of the cold energy recovery circuit is in heat exchange with the rotating cabin.

[0017] Part of the cold energy recovery circuit is spirally wound and extends above the rotating cabin.

[0018] The refrigeration cycle device, sample library system and control method of the full-automatic ultralow-temperature storage equipment have the advantages that:

[0019] 1. The refrigeration cycle device adopts the uniform distribution type coil pipe design, and adopts the compressor refrigeration or the compressor main refrigeration liquid nitrogen auxiliary refrigeration mode to replace the traditional liquid nitrogen evaporation refrigeration mode, so that high pressure is not generated in the storage cabin due to liquid nitrogen evaporation, the temperature of the storage cabin can be conveniently controlled, the structure of the refrigeration cycle device is convenient for maintenance, the biological sample can be conveniently taken out and transferred when the equipment fails, and the safety of biological sample storage is further improved.

[0020] 2. The rotating cage structure of the refrigeration cycle device is provided with double-length storage grooves, so that a single storage groove can accommodate two biological sample boxes, the number of samples that can be stored in the rotating cage can be significantly improved without increasing the size of the equipment, meanwhile, the distribution of the storage cabin temperature sensors is more uniform, and the monitoring accuracy of the temperature in the storage cabin is further improved. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 It is the appearance structure diagram of the ultralow-temperature biological storage equipment;

[0022] Figure 2 It is Figure 1 It is the structure diagram after the side plate is hidden;

[0023] Figure 3 It is one of the internal structure diagrams of the storage cabin and the rotating cabin;

[0024] Figure 4 It is the second of the internal structure diagrams of the storage cabin and the rotating cabin;

[0025] Figure 5 It is the internal structure diagram of the rotating cabin;

[0026] Figure 6 is one of the structure schematic diagram of the rotating cage described in this embodiment (only two shelves are shown);

[0027] Figure 7 is the second structure schematic diagram of the rotating cage described in this embodiment;

[0028] Figure 8 is the cooperation structure schematic diagram of the transfer driving assembly and the sampling member described in this embodiment;

[0029] Figure 9 is the structure schematic diagram of the sampling member described in this embodiment;

[0030] Figure 10 is the structure schematic diagram of the compressor refrigeration circuit, the liquid nitrogen refrigeration circuit and the cold energy recovery circuit described in this embodiment;

[0031] Figure 11 is the structure schematic diagram of the compressor refrigeration circuit and the liquid nitrogen refrigeration circuit described in the embodiment 2 of the utility model (only a single circuit structure is shown).

[0032] 1, full-automatic ultralow-temperature storage equipment main structure;11, storage cabin;12, transfer cabin;121, sample inlet;2, storage control device;21, rotating cage;211, top cover;212, base;213, shelf;22, rotating cage support;23, storage driving assembly;3, transfer device;31, transfer driving assembly;311, lifting mechanism;312, telescopic mechanism;32, sample moving assembly;33, sampling member;331, material shoveling section;34, workbench;341, material moving port;342, cold trap;35, push-pull device;36, code scanning device;4, first refrigeration device;41, liquid nitrogen refrigeration circuit;42, compressor refrigeration circuit;43, circulating fan blade;5, cold energy recovery circuit;6, transfer door;7, transfer tank;8, biological sample box. DETAILED DESCRIPTION

[0033] In order to make the purpose, characteristics and advantages of the utility model more obvious and easy to understand, the technical solutions in the embodiments of the utility model will be described clearly and completely in combination with the drawings in the embodiments of the utility model. Obviously, the following described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the utility model. EMBODIMENT

[0034] The embodiment discloses a refrigeration circulating device of full-automatic ultralow-temperature storage equipment, which comprises:

[0035] The application discloses an ultra-low temperature biological storage device, which comprises a main body structure 1 in the shape of a cuboid in an integrated structure for facilitating transportation, the main body structure 1 is hollow inside and has two areas, respectively provided with a storage cabin 11 and a transfer cabin 12 arranged side by side, and a transfer channel with controllable opening and closing is arranged between the storage cabin 11 and the transfer cabin 12, the transfer channel comprises a plurality of transfer windows formed on one side of the storage cabin 11 close to the sampling element 33, the plurality of transfer windows are arranged along the height direction, a transfer door 6 with independent controllable opening and closing is arranged on each transfer window, and the transfer door 6 is controlled to open and close by an external cylinder, specifically, the transfer channel is a through opening formed on the side plate of the storage cabin 11 close to the transfer cabin 12, the through opening is in the shape of a long strip and is arranged along the height direction of the main body structure 1. Figure 2 As shown in the figure, the sampling access port 121 is arranged on the side surface of the front end of the main body structure 1.

[0036] The storage cabin 11 is innovatively in a non-Dewar tank type structure, the plate structure of the storage cabin 11 comprises a base structure and a composite thermal insulation layer combined with the base structure, the base structure is a stainless steel plate, and the composite thermal insulation layer comprises an aerogel layer, an aluminum foil, a vacuum thermal insulation plate and an elastic thermal insulation layer which are sequentially stacked from inside to outside, and the elastic thermal insulation layer is specifically an elastic fiber felt or an elastic fiber felt with an aluminum foil, the structure of the storage cabin 11 can replace the traditional Dewar tank type, can meet the heat insulation requirement, and can effectively reduce the equipment volume, weight and equipment cost.

[0037] The storage cabin 11 is internally provided with a storage control device 2, the storage control device 2 comprises a storage container, a rotating cage 21 support and a storage driving assembly 23, the storage container is the rotating cage 21, the rotating cage 21 is rotationally arranged on the rotating cage 21 support, and the storage driving assembly 23 is linked with the rotating cage 21 and used for driving the rotating cage 21 to rotate; the storage container is rotationally arranged in the storage cabin 11 and has a plurality of storage areas for storing biological samples.

[0038] Further, the rotating cage 21 comprises a top cover 211, a base 212 and a plurality of strip-shaped storage racks 213, the top cover 211 and the base 212 are both in the shape of a disc and are arranged in parallel to each other, the plurality of strip-shaped storage racks 213 are vertically arranged and are distributed in a circumferential array between the top cover 211 and the base 212 around the center of the base 212.

[0039] The storage rack 213 has a plurality of storage slots arranged along the height direction thereof, the storage slots extend along the radial direction of the base 212, and the extension length of at least half of the storage slots of the storage rack 213 is at least twice the length of the biological sample box 8, so as to simultaneously store at least two biological sample boxes 8; the opening of the storage slot is outwardly directed along the radial direction of the base 212; the extension length of at least half of the storage slots of the storage rack 213 is at least twice the length of the biological sample box 8, so as to simultaneously store at least two biological sample boxes 8; in a specific embodiment, the extension length of half of the storage slots of the storage rack 213 is twice the length of the biological sample box 8, and the storage racks 213 are arranged in a spaced distribution manner; the storage rack 213 on which a single biological sample box 8 can be placed in a single layer is defined as a single-layer storage rack 213, and the storage rack 213 on which two biological sample boxes 8 can be placed in a single layer is defined as a double-layer storage rack 213; that is, the storage racks 213 are arranged in a single-layer storage rack-double-layer storage rack alternating manner, so that the storage capacity of the storage cabin 11 can be increased by 50% without changing the size of the original equipment

[0040] The rotating cage 21 support is a hollow multi-prism support structure, so that the rotating cage support 22 as a whole has a polyhedral structure, such as tetrahedron, pentahedron, hexahedron, octahedron and other polyhedral structures. This structure can facilitate the arrangement of multiple groups of corresponding radiation refrigeration coils, enhance the temperature transfer speed, and enable the support to have stable internal stress performance, so that the overall structure of the support is stable. The storage cabin 11 is provided with a supporting bottom plate corresponding to the position of the rotating cage 21 support for supporting the rotating cage 21 support. The storage cabin 11 is provided with a supporting bottom plate corresponding to the position of the rotating cage support 22 for supporting the rotating cage support 22. The supporting bottom plate supports the rotating cage support 22, and in turn supports the whole rotating cage device. The bottom supporting mode replaces the traditional suspension structure, which can improve the safety and stability of the equipment.

[0041] The refrigeration cycle device includes a first refrigeration system 4 for heat exchange with the storage cabin 11, a second refrigeration system for heat exchange with the transfer cabin 12, a third refrigeration system for heat exchange with the cold trap 342, a first temperature monitoring module signal connected with the first refrigeration system 4, a second temperature monitoring module signal connected with the second refrigeration system, and a third temperature monitoring module signal connected with the third refrigeration system.

[0042] The first refrigeration system 4 includes a refrigeration pipeline and a circulating fan blade 43. The refrigeration pipeline is arranged outside the rotating cage 21 for radiating refrigeration to the rotating cage 21. The circulating fan blade 43 is arranged in the middle of the rotating cage 21. The rotation of the circulating fan blade 43 is controlled by a variable frequency motor, which is used to drive the airflow to flow uniformly in the storage cabin 11.

[0043] The circulating fan blade 43 comprises a central rotating shaft and a plurality of fan blades fixed concentrically on the central rotating shaft, wherein the fan blades are made of low-temperature-resistant tetra-propyl fluoride rubber, the central rotating shaft is driven by a variable frequency motor, and the fan blades rotate with the central rotating shaft; the fan blades in the embodiment are made of tetra-propyl fluoride rubber, which can maintain good softness and elasticity in an ultra-low temperature environment, has excellent low-temperature resistance, and is not easy to become brittle or lose elasticity;

[0044] The circulating fan blade 43 rotates in the storage cabin 11, so that the cold air flow in the storage cabin 11 circulates to achieve good refrigeration uniformity;

[0045] The refrigeration principle of the first refrigeration system 4 is as follows: the compressor cools the compressor refrigeration circuit 42 to preliminarily cool the storage cabin 11; when the operation of the compressor tends to be stable, liquid nitrogen is introduced into the lower end of the liquid nitrogen refrigeration circuit 41, the return pipeline of the liquid nitrogen refrigeration circuit 41 is arranged to be bent at the top of the rotating cage support 22, the liquid nitrogen forms a return flow, and the return flow circulates to further cool the storage cabin 11, until the temperature inside the storage cabin 11 is reduced to-196℃ and kept at-196℃ with a certain degree of temperature fluctuation; wherein the compressor preliminarily cools the storage cabin 11 to make the temperature inside the storage cabin 11 fluctuate at-80℃, the liquid nitrogen is further introduced to cool the storage cabin 11 to reduce the temperature to fluctuate at-196℃, and the amount of liquid nitrogen introduced into the liquid nitrogen refrigeration circuit 41 is controlled as a refrigeration means;

[0046] The first temperature monitoring module comprises a plurality of first temperature sensors arranged inside the storage cabin 11; the second temperature monitoring module comprises a plurality of second temperature sensors arranged inside the transfer cabin 12; and the third temperature monitoring module comprises a plurality of third temperature sensors arranged inside the cold trap 342.

[0047] Further, part of the first temperature sensors are distributed on the prism centripetal side of the rotating cage 21 support, the remaining first temperature sensors are distributed on the side wall of the transfer door 6 facing the storage cabin 11, and the temperature sensors of the upper and lower adjacent storage cabins 11 are spaced apart at a height consistent with the height of the storage groove; the second temperature sensors are arrayed on the table top 34; and the third temperature sensors are distributed on the inner wall of the cold trap 342.

[0048] The refrigeration circulating device further comprises a cold energy recovery circuit 5, one end of the cold energy recovery circuit 5 is connected with the return flow end of the liquid nitrogen refrigeration circuit 41 through an electromagnetic valve, at least part of the cold energy recovery circuit 5 is in heat exchange with the transfer cabin 12; part of the cold energy recovery circuit 5 is spirally coiled and extends above the transfer cabin 12;

[0049] After the refrigeration inside the storage cabin 11 is completed by the liquid nitrogen refrigeration circuit 41, the liquid nitrogen in the liquid nitrogen refrigeration circuit 41 still has sufficient cold energy, and the low-temperature requirement of the front-end transfer cabin 12 is relatively low, only about -20°C. The cold energy is recovered for refrigeration of the front-end transfer cabin 12. Specifically, a cold energy recovery circuit 5 is arranged, one end of the cold energy recovery circuit 5 is connected with the return end of the liquid nitrogen refrigeration circuit 41, and the other end extends into the transfer cabin 12 and is coiled into a spiral shape at the top of the transfer cabin 12. In this way, the liquid nitrogen refrigerated by the liquid nitrogen refrigeration circuit 41 flows into the transfer cabin 12 through the cold energy recovery circuit 5, and irradiates and refrigerates the transfer cabin 12 from top to bottom, so that the cold energy of the liquid nitrogen can be fully utilized.

[0050] The transfer cabin 12 is internally provided with a workbench 34, and the second refrigeration system is internally provided in the transfer cabin 12. The second refrigeration system is a refrigeration compressor. The workbench 34 has an import and export cabin for accommodating the transfer tank 7. The hatch of the import and export cabin corresponds to the aforementioned sample access port 121. The workbench 34 has a material moving port 341 communicating with the import and export cabin. The material moving port 341 penetrates from top to bottom. The workbench 34 is provided with a transfer device 3.

[0051] The transfer device 3 includes a push-pull device 35, a transfer driving assembly 31, a sample moving assembly 32, and a sample taking member 33. The transfer driving assembly 31 is linked with the sample taking member 33 to drive the sample taking member 33 to extend into the storage area to take and place the biological sample. The sample moving assembly 32 is used to transfer the biological sample between the sample taking member 33, the cold trap 342, and the sample access port 121. The push-pull device 35 is slidingly arranged in the import and export cabin, so that the push-pull device 35 can be pulled outwards to outside the workbench 34. The push-pull device 35 is used to carry the transfer tank 7 containing the biological sample box 8, so as to serve as a connection channel between the transfer cabin 12 and the outside. The outer end of the push-pull device 35 is provided with a baffle for covering and shielding the sample access port 121.

[0052] When the push-pull device 35 is withdrawn into the inside, the baffle shields the sample access port 121 to make the transfer cabin 12 closed. When the baffle is pulled outwards, the sample access port 121 is opened. In a specific embodiment, the push-pull device 35 can be driven to move in the manner of an electric cylinder / gas cylinder.

[0053] The workbench 34 is further provided with a cold trap 342 located on the workbench 34 and on one side of the material moving port 341, and the third refrigeration system is arranged in the cold trap 342, and a plurality of spray holes for spraying liquid nitrogen or gaseous low-temperature nitrogen are formed on the side of the cold trap 342, the spray holes are in communication with a liquid nitrogen source, and after the biological sample tube is placed in the cold trap 342, liquid nitrogen or gaseous low-temperature nitrogen is sprayed through the spray holes to keep the cold trap 342 at a low temperature, so that the sample is not damaged; a code scanning device 36 is further arranged on the side of the cold trap 342.

[0054] Further, the transfer driving assembly 31 comprises a lifting mechanism 311 and a telescopic mechanism 312, the sampling member 33 is a shovel disc, one section of the shovel disc is a material shoveling section 331, the length of the material shoveling section 331 is at least twice the length of the biological sample box 8, and the telescopic mechanism 312 is linked with the shovel disc and is used for driving the shovel disc to extend into the storage area or to exit outwardly out of the storage area; the lifting mechanism 311 is linked with the telescopic mechanism 312 and is used for driving the telescopic mechanism 312 and the shovel disc to ascend and descend.

[0055] The sample moving assembly 32 is a mechanical arm arranged on the workbench 34 and on the side of the material moving port 341; the sample moving assembly 32 is a multi-degree-of-freedom mechanical arm arranged on the workbench 34 and on the side of the material moving port 341, and is provided with a plurality of clamps such as a cap clamp, a sample box clamp and a tube taking clamp and the like, and the clamps can be switched through a clamp switching mechanism; the mechanical arm is used for opening the cap of the transfer tank 7 after the transfer tank 7 is placed, taking out the sample box in the transfer tank 7, moving the sample box to the cold trap 342 to take the tube into the warehouse, and then grabbing the sample box to move to the sampling member 33, and then driving the sampling member 33 to enter the warehouse by the transfer driving assembly 31.

[0056] The code scanning device 36 has the functions of scanning the side box code and the bottom code of the batch sample cryogenic tube, and checking the codes of the sample box and the sample cryogenic tube that are placed or taken out.

[0057] In the present application, the required constant temperature value of the storage cabin 11 is-196 DEG C, and the required constant temperature value of the transfer cabin 12 is-20 DEG C, which are refrigerated by the first refrigeration system 4 and the second refrigeration system respectively.

[0058] Further, the full-automatic ultralow-temperature biological storage equipment further comprises:

[0059] The host computer is provided with platform software for accessing a third-party platform and for storing in-and-out warehouse data;

[0060] A controller is in signal connection with the host computer;

[0061] The full-automatic ultralow-temperature biological storage device has a single-machine mode and an online mode,

[0062] In the single-machine mode, the controller executes corresponding actions according to the instructions of the host computer;

[0063] In the online mode, the host computer accesses a third-party platform and can execute corresponding actions according to remote control instructions and can communicate with and coordinate actions of other full-automatic ultralow-temperature biological storage devices or matched devices.

[0064] Compared with the sample library system in the prior art, the multiple ultralow-temperature biological storage devices in combination with the refrigeration circulating device have the following advantages:

[0065] The single storage device has a small volume, can freely enter and exit an elevator, is convenient to maintain, does not need to be installed on site, can be used by being plugged in, can be suitable for various cooling modes such as air cooling and water cooling, and has good compatibility;

[0066] In the case that the storage space is unchanged, the storage capacity is increased, the storage cost of a single sample is lower than that of the existing sample library, the construction period is short, and the one-time investment cost and the early operation cost can be significantly reduced;

[0067] The operation risk is small, since the sample library system is composed of multiple independent single units, the loss risk is small, and in the case of unexpected situations, the safety performance is significantly better than that of the existing sample library. Embodiment

[0068] The embodiment discloses a refrigeration device applying a contrast type refrigeration pipeline arrangement;

[0069] The first refrigeration system 4 comprises a liquid nitrogen refrigeration circuit 41 and a compressor refrigeration circuit 42, both of which are in heat exchange with the storage cabin for jointly refrigerating the inside of the storage cabin; the refrigeration principle of the first refrigeration system 4 is that the compressor cools the compressor refrigeration circuit 42 to preliminarily cool the storage cabin 11, and when the work of the compressor tends to be stable, liquid nitrogen is introduced into the lower end of the liquid nitrogen refrigeration circuit 41, and the return pipeline of the liquid nitrogen refrigeration circuit 41 is bent at the top of the rotating cage support 22 to make the liquid nitrogen form a return flow for circulation to further cool the storage cabin 11 until the temperature inside the storage cabin 11 is reduced to -196℃ and kept at -196℃ with a certain degree of temperature fluctuation; wherein the compressor preliminarily cools the storage cabin 11 to make the temperature inside fluctuate at -80℃, and the liquid nitrogen is further introduced to cool the storage cabin 11 to reduce the temperature to fluctuate at -196℃, and the amount of liquid nitrogen introduced into the liquid nitrogen refrigeration circuit 41 is controlled as a refrigeration means;

[0070] Further, the refrigeration pipeline comprises at least one set of refrigeration coils, two refrigeration coils of the same set are oppositely arranged on the two sides of the rotating cage support 22, the refrigeration pipeline comprises a liquid nitrogen refrigeration circuit 41 and a compressor refrigeration circuit 42, both of which are in heat exchange with the storage cabin 11, and the liquid nitrogen refrigeration circuit 41 and the compressor refrigeration circuit 42 are alternately arranged along the height direction of the rotating cage 21 (as shown in Figure 11

[0071] The circulating fan blade 43 is arranged in the middle of the storage container, and the rotation of the circulating fan blade 43 is controlled by a variable frequency motor to drive the airflow to flow uniformly in the storage cabin;

[0072] The circulating fan blade 43 comprises a central shaft and a fan blade, a plurality of fan blades are fixed concentrically on the central shaft, the fan blade is made of low-temperature-resistant tetrafluoroethylene rubber, the central shaft is driven by a variable frequency motor, and the fan blade rotates with the central shaft; the fan blade in the embodiment is made of tetrafluoroethylene rubber, which can maintain good softness and elasticity in an ultra-low temperature environment, has excellent low-temperature resistance, and is not easy to become brittle or lose elasticity;

[0073] The rotation of the circulating fan blade 43 in the storage cabin 11 can make the cold airflow in the storage cabin 11 circulate to achieve good refrigeration uniformity;

[0074] The circulating fan blade 43 can be adjusted in speed to adjust the airflow circulation speed when there is a temperature difference of different degrees at different positions in the storage cabin 11.​

Claims

1. A refrigeration cycle device of a fully automatic ultra-low temperature storage device, the ultra-low temperature storage device comprising a storage cabin (11) and a transfer cabin (12), a transfer channel being formed between the storage cabin (11) and the transfer cabin (12), the storage cabin (11) being provided with a transfer cage (21) for placing a biological sample box and a transfer cage support (22) for setting the transfer cage (21), the transfer cabin (12) being provided with a cold trap (342) for performing a tube picking work; characterized in that: The refrigeration cycle device comprises a first refrigeration system (4) in heat exchange with the storage cabin (11), the first refrigeration system (4) comprising refrigeration pipelines arranged outside the rotating cage (21) for radiating refrigeration to the rotating cage (21) and circulating air blades (43) arranged in the middle part of the rotating cage (21).

2. The refrigeration cycle apparatus of a fully automatic ultra-low temperature storage equipment according to claim 1, characterized in that: The refrigeration pipelines are arranged outside the rotating cage support (22) in a coiled manner, and a gap for the biological sample box to enter and exit is reserved at a position corresponding to the transfer channel.

3. The refrigeration cycle apparatus of the fully automatic ultra-low temperature storage equipment according to claim 1, characterized in that: The refrigeration pipelines comprise at least one set of refrigeration coils, and two refrigeration coils of the same set are oppositely arranged on both sides of the rotating cage support (22).

4. A refrigeration cycle apparatus for a fully automatic ultra-low temperature storage device according to any one of claims 1 to 3, characterized in that: The refrigeration pipelines comprise a liquid nitrogen refrigeration circuit (41) and a compressor refrigeration circuit (42), both of which are in heat exchange with the storage cabin (11), and the liquid nitrogen refrigeration circuit (41) and the compressor refrigeration circuit (42) are alternately arranged along the height direction of the rotating cage (21).

5. The refrigeration cycle apparatus of the fully automatic ultra-low temperature storage equipment according to claim 1, characterized in that: The refrigeration cycle device further comprises a second refrigeration system in heat exchange with the transfer cabin (12), a third refrigeration system in heat exchange with the cold trap (342), a first temperature monitoring module in signal connection with the first refrigeration system (4), a second temperature monitoring module in signal connection with the second refrigeration system, and a third temperature monitoring module in signal connection with the third refrigeration system.

6. A refrigeration cycle apparatus of a fully automatic ultra-low temperature storage device according to claim 5, characterized in that: The first temperature monitoring module comprises a plurality of first temperature sensors arranged inside the storage cabin (11); the second temperature monitoring module comprises a plurality of second temperature sensors arranged inside the transfer cabin (12); and the third temperature monitoring module comprises a plurality of third temperature sensors arranged inside the cold trap (342).

7. A refrigeration cycle apparatus of a fully automatic ultra-low temperature storage device according to claim 6, characterized in that: Some of the first temperature sensors are distributed on the prism centripetal side of the rotating cage support (22), and the remaining first temperature sensors are distributed on the side wall of the transfer door (6) facing the storage cabin (11), and the interval between the upper and lower adjacent first temperature sensors is consistent with the height of the storage groove; the second temperature sensors are arrayed and arranged on the table top of the workbench (34); and the third temperature sensors are distributed and arranged on the inner wall of the cold trap (342).

8. The refrigeration cycle apparatus of the fully automatic ultra-low temperature storage equipment according to claim 1, characterized in that: The refrigeration cycle device further comprises a cold energy recovery circuit (5), one end of the cold energy recovery circuit is connected with the backflow end of the liquid nitrogen refrigeration circuit (41) through an electromagnetic valve, at least part of the cold energy recovery circuit (5) is in heat exchange with the transfer cabin; and part of the cold energy recovery circuit (5) is coiled in a spiral manner and extends above the transfer cabin (12).

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