Condensate water removing and cleaning device

Abstract

The utility model provides a condensed water removing and cleaning device, and relates to the technical field of experiment auxiliary equipment, the condensed water removing and cleaning device comprises a column body, a liquid transferring part, a liquid absorbing module and a wiping module, the column body comprises a cavity, the liquid transferring part is arranged in the cavity, the lower end of the column body is connected with the liquid absorbing module, the liquid absorbing module comprises a cavity and a liquid absorbing head which are communicated, and the cavity is communicated with the liquid absorbing head. The liquid suction head is arranged on the lower side of the cavity, the cavity is communicated with the cavity, the wiping module is detachably connected with the lower end of the liquid suction module, and a flexible wiping part is arranged on the lower side of the wiping module. According to the technical scheme, the technical problem of how to effectively clean small holes in experimental equipment and remove condensate water in the prior art is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of experimental auxiliary equipment, in particular to a device for removing condensate and cleaning. BACKGROUND

[0002] With the improvement of technology, more and more pipetting workstations (such as library construction workstations) enter the laboratory, and small holes with a volume of 100 or 200 uL are not only used for PCR heating modules, but more are used for low-temperature refrigeration storage of reagents or as intermediate devices with low-temperature function for adding samples and reagents. However, the low temperature also causes a large amount of condensate in the holes, and it is difficult to remove the condensate in the holes. The insufficient cleaning of the holes and the long-term existence of the condensate will affect the service life of consumables. SUMMARY

[0003] The present application provides a device for removing condensate and cleaning to solve the technical problem of how to effectively clean small holes in experimental equipment and remove condensate in the prior art. The preferred technical solutions in the many technical solutions provided by the present application can produce many technical effects, which are described in detail below.

[0004] To achieve the above-mentioned purpose, the present application provides a device for removing condensate and cleaning, comprising: a column, a pipetting component, a liquid suction module and a wiping module, the column comprises a cavity, the pipetting component is arranged in the cavity, the lower end of the column is connected with the liquid suction module, the liquid suction module comprises a communicating chamber and a liquid suction head, the liquid suction head is arranged at the lower side of the chamber, the chamber is in communication with the cavity, the wiping module is detachably connected with the lower end of the liquid suction module, and a flexible wiping part is arranged at the lower side of the wiping module.

[0005] Optionally, the pipetting component comprises a piston and a spring, and the spring is arranged between the piston and the column to keep the piston in the initial position.

[0006] Optionally, the piston is flush with the upper end of the column at the end of the upper end of the column, and a button opening is arranged on the side surface of the upper end of the column.

[0007] Optionally, the lower end of the column is provided with a liquid suction column.

[0008] Optionally, an outer thread is arranged on the outer side surface of the liquid suction column, and the liquid suction module is connected with the column through the outer thread.

[0009] Optionally, the liquid suction head is conical.

[0010] Optionally, the tip of the liquid suction head is provided with a cross-shaped opening.

[0011] Optionally, the upper side of the wiping module is provided with a conical opening matching the shape of the conical liquid suction head.

[0012] Optionally, the flexible wiping part is provided in a conical or cylindrical sponge shape.

[0013] Optionally, the liquid suction head of the liquid suction module is provided in a plurality, and the conical opening on the wiping module is provided one-to-one with the liquid suction head.

[0014] In order to make the features and advantages of the present application more obvious and easy to understand, some embodiments are described below, and the accompanying drawings are described in detail as follows. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.

[0016] Figure 1 is a structure schematic diagram of the Chinese library construction workstation of the embodiment of the present application;

[0017] Figure 2 is a structure schematic diagram of the condensate water and cleaning device of the embodiment of the present application;

[0018] Figure 3 is a sectional view of the condensate water and cleaning device of the embodiment of the present application;

[0019] Figure 4 is a structure schematic diagram of the liquid suction head opening of the condensate water and cleaning device of the embodiment of the present application;

[0020] Figure 5 is a structure schematic diagram of the liquid suction module of the condensate water and cleaning device of the embodiment of the present application;

[0021] Figure 6 is a structure schematic diagram of the condensate water and cleaning device of the embodiment of the present application corresponding to the deep well plate.

[0022] In the figure: 20, library construction workstation; 21, PCR instrument; 22, file construction actual container; 23, shaking heating assembly; 24, magnet assembly; 25, piercing assembly working area; 26, waste container; 27, large-capacity reagent container; 101, column; 1011, cavity; 1012, piston; 10121, button; 1013, liquid suction column; 1014, spring; 1015, button opening; 102, liquid suction module; 1021, chamber; 1022, liquid suction head; 10221, cross opening; 103, wiping module; 1031, tapered opening; 1032, flexible wiping part; 201, deep well plate; 2011, well site; 202, condensation module. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical scheme and advantages of the present application clearer, the technical scheme of the present application will be described in detail below. Obviously, the described embodiments are only some of the embodiments of the present application, not all. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor belong to the scope of protection of the present application.

[0024] It should be noted that when an element is referred to as "fixed to" or "disposed on" another element, it can be directly on the other element or there can be a middle element. When an element is referred to as "connected to" another element, it can be directly connected to the other element or there can be a middle element.

[0025] In the second generation gene sequencing (NGS, Next Generation Sequencing) technology, the nucleic acid molecules (such as DNA or RNA) extracted from biological samples need to be broken by physical or enzymatic methods, for example, broken by ultrasonic waves, and after breaking, the nucleic acid fragments are first filled with enzymes at both ends, and then the fragments are connected with specific DNA sequences (usually, this specific DNA sequence is also called a linker) at both ends by a specific enzyme, and finally the nucleic acid fragments are formed, which are called libraries in the industry.

[0026] In order to save sequencing costs, generally, multiple biological samples will be sequenced simultaneously in a sequencer, and in order to distinguish the sequencing results of different samples, a segment of DNA sequence (usually containing 6-8 bases) that can identify the source of the sample will be included in the linker when preparing the library of the sample, which is also called a sample tag (or Index, Barcode). It can be understood that each library linker of each sample contains its exclusive sample tag. For the library construction workstation 20, as shown in Figure 1 the library construction workstation 20 at least includes a PCR instrument 21, a library construction operation hand assembly (Figure 1 only its working area), a library construction reagent container 22, a shaking and heating assembly 23, and a magnet assembly 24. It is to be noted that, Figure 1 only its working area), a library construction reagent container 22, a shaking and heating assembly 23, and a magnet assembly 24. It is to be noted that, Figure 1 are only schematic and should not be construed as limiting the position of the parts.

[0027] The library construction reagent container 22 (e.g., a reagent kit) can contain one or more reagents required for library construction, and the library construction reagent container 22, the shaking and heating assembly 23, and the magnet assembly 24 are respectively located in their respective storage areas, and each of the storage areas is located within the working area of the library construction operator assembly and the library construction pipetting assembly. The storage area of the library construction reagent container 22 generally needs to be in a low-temperature refrigeration environment to facilitate low-temperature refrigeration of the library construction reagents. The shaking and heating assembly 23 is provided with a purification container (e.g., a deep-well plate) and a shaking and heating device, the purification container can provide a reaction space for magnetic bead purification in the library construction process, and the shaking and heating device can provide suitable reaction temperature and reaction conditions for the magnetic bead purification. Meanwhile, the magnet assembly 24 can provide the necessary magnetic field environment for the magnetic bead purification.

[0028] After the nucleic acid extraction quality control is completed, the library construction operator assembly is responsible for taking the construction reaction container (to which the nucleic acid extraction product has been added) from the quality control system 30 and placing it in a specific storage area in the library construction system 20, in which area various reaction reagents are added to the construction reaction container. For the convenience of description, this storage area can be referred to as a reagent addition area. Since the construction reaction container contains nucleic acid, the reagent addition area generally needs to be in a low-temperature refrigeration environment to facilitate low-temperature refrigeration of the nucleic acid. Since the entire library construction process includes multiple reaction steps, for each reaction step, the library construction pipetting assembly is responsible for first drawing the corresponding reaction reagent from the library construction reagent container 22 and adding it to the construction reaction container (to which the nucleic acid extraction product has been added), and then the library construction operator assembly is responsible for placing the construction reaction container (to which the nucleic acid extraction product and the corresponding reaction reagent have been added) into the PCR instrument 21 for reaction. After the reaction is completed, the library construction operator assembly takes the construction reaction container out of the PCR instrument 21 and places it back into the specific area to wait for the addition of the reaction reagent for the next reaction, and the above process is repeated.

[0029] In the library construction process, after the adapters are ligated to the ends of the nucleic acid fragments, the ligation products need to be purified to obtain a pure library. In addition, due to the small amount of library obtained at this time, library amplification is usually required, and after library amplification, the amplification products also need to be purified to obtain a pure library. If magnetic bead purification is used, in addition to being able to accommodate one or more reagents required for library construction (including washing solution and elution solution required for magnetic bead purification) in the library construction reagent container 22, magnetic beads or other magnetic particles can also be accommodated. During the magnetic bead purification reaction of the ligation product or the amplification product, the library construction pipetting assembly is responsible for first sucking the ligation product / amplification product from the construction reaction container and adding it to the purification container of the shaking and heating assembly 23, then sucking the magnetic beads from the library construction reagent container 22 and adding them to the purification container (where the ligation product / amplification product has been added), after a certain period of reaction, the library construction operation hand assembly is responsible for transferring the purification container (where the library is adsorbed on the surface of the magnetic beads) to the magnet assembly 24, under the action of the magnetic field, the magnetic beads adsorbed with the library are separated from the solution, and the library construction pipetting assembly sucks the supernatant in the purification container. Continue to suck out the magnetic beads adsorbed with the library. The library construction pipetting assembly continues to suck the washing solution from the library construction reagent container 22 and add it to the purification container, after a certain period of reaction, the supernatant is sucked out again. According to the experimental requirements, the library construction pipetting assembly can be repeatedly operated for multiple times of washing, after washing is completed, the library construction operation hand assembly transfers the purification container to the shaking and heating assembly 23, and the library construction pipetting assembly continues to suck the elution solution from the library construction reagent container 22 and add it to the purification container. After a certain period of reaction, the library and the magnetic beads are desorbed, and the library construction pipetting assembly sucks the supernatant (i.e., the library construction product) in the purification container and adds it to the construction reaction container.

[0030] Generally, a sealing film will be attached to the upper surface of the library construction reagent container 22 for the preservation and transportation of the internal reagents. After the reagent container is loaded into the inside of the pre-sequencing treatment instrument, the sealing film needs to be punctured by the puncturing assembly (only the working area thereof is shown) to facilitate the corresponding pipetting assembly to suck the reagents from the library file reagent container 22, and the storage area of the library construction reagent container 22 is located within the working area 25 of the puncturing assembly. Figure 1 In addition, considering that the volume of magnetic beads, washing solution, and elution solution required in the magnetic bead purification process is large and does not need to be in a low-temperature refrigeration environment, in addition to the library construction reagent container 22, the library construction system 20 can further be provided with a large-capacity reagent container 27 for storing large-capacity reagents such as magnetic beads, washing solution, and elution solution.

[0031]

[0032] ​It should be noted that the library construction operation hand assembly and the library construction pipetting assembly can be independently arranged, and considering that the library construction operation hand assembly and the library construction pipetting assembly do not need to work in parallel, the two can also be integrated for space saving. In addition, the library construction system 20 can batch process nucleic acid extraction products of multiple biological samples.

[0033] In an implementation manner, the library pipetting assembly can be a pipetting pump, and a pipetting head for pipetting is also provided for the pipetting pump. When the pipetting work starts, the pipetting pump covers the pipetting head on the end thereof, and after the pipetting work is completed, the pipetting pump separates the pipetting head from the end thereof. Correspondingly, the library construction system 20 is also provided with a pipetting head storage area, and the used pipetting head is put into the waste container 26, as shown in Figure 1 .

[0034] The storage area (refrigeration module) of the library construction reagent container 22 generally needs to be in a low-temperature refrigeration environment to facilitate low-temperature refrigeration of the library construction reagent. The low-temperature environment of the refrigeration module causes a large amount of condensate water to exist in the refrigeration holes of the module, and the condensate water in the holes is difficult to remove due to the small hole volume (100 or 200 uL) and exists for a long time, which affects the service life of consumables. In order to solve the problems of cleaning and condensate water removal of the deep well plate, a cleaning tool that can remove condensate water and clean the deep well plate is needed.

[0035] To solve the technical problem, the application provides a condensate water removal and cleaning device.

[0036] As shown in Figure 2 , Figure 3 The application provides a condensate water removal and cleaning device, which comprises a column 101, a pipetting component, a liquid suction module 102 and a wiping module 103. The column 101 comprises a cavity 1011, the pipetting component is arranged in the cavity, the lower end of the column 101 is connected with the liquid suction module 102, the liquid suction module 102 comprises a communicating cavity 1021 and a liquid suction head 1022, the liquid suction head 1022 is arranged at the lower side of the cavity 1021, the cavity 1021 communicates with the cavity 1011, the wiping module 103 is detachably connected with the lower end of the liquid suction module 102, and a flexible wiping part 1032 is arranged at the lower side of the wiping module 103.

[0037] Specifically, by arranging the internally communicating column 101 and the liquid suction module 102, the pipetting component is arranged in the column 101, the condensate water in the hole position 2011 of the deep well plate 201 to be cleaned can be sucked and removed, the wiping module 103 is detachably arranged at the lower end of the liquid suction module 102, and the flexible wiping part 1032 is used to clean the device to be cleaned.

[0038] As an optional implementation, the pipetting component includes a piston 1012 and a spring 1014 arranged between the piston 1012 and the cylinder to keep the piston 1012 in the initial position.

[0039] Specifically, the pipetting component is composed of a depressible piston 1012 and a retractable spring 1014; by pressing the piston 1012, the piston 1012 moves downward to complete the liquid discharge action; under the action of the spring 1014, the piston 1012 moves upward to return to the original position, i.e., the initial position, to complete the liquid suction action.

[0040] As an optional implementation, the piston 1012 is flush with the upper end of the cylinder, and a button opening 1015 is arranged on the side surface of the upper end of the cylinder.

[0041] Specifically, the upper end of the piston 1012 is provided with a button 10121 flush with the upper end of the cylinder, and the cylinder is provided with a button opening 1015 for operating the button 10121, and the flush part of the cylinder and the piston 1012 can prevent the button 10121 from being accidentally operated during operation.

[0042] As an optional implementation, a liquid suction column 1013 is arranged at the lower end of the cylinder.

[0043] Specifically, the liquid suction column 1013 at the lower end of the cylinder facilitates liquid discharge and suction when the piston 1012 moves.

[0044] As an optional implementation, an external thread is arranged on the outer side surface of the liquid suction column 1013, and the liquid suction module 102 is connected to the cylinder 101 through the external thread.

[0045] Specifically, the liquid suction module 102 is connected to the cylinder 101 through the external thread on the outer side of the liquid suction column 1013, which facilitates disassembly and connection operation.

[0046] As an optional implementation, the liquid suction head 1022 is conical.

[0047] Specifically, the structure of the liquid suction head 1022 is arranged corresponding to the structure of the device to be cleaned, and for cleaning the hole site of the deep well plate 201, the liquid suction head 1022 is arranged as a cone, which facilitates the suction of condensate water in the hole site of the deep well plate 201.

[0048] As an optional implementation, the tip of the liquid suction head 1022 is provided with a cross opening 10221.

[0049] Specifically, as shown in Figure 4 , Figure 5 , the tip of the liquid suction head 1022 is provided with a cross opening 10221 structure to avoid the suction head being blocked and unable to suck liquid.

[0050] As an optional implementation, the upper side of the wiping module 103 is provided with a conical opening 1010 which is shaped to match the conical liquid suction head 1022.

[0051] Specifically, the wiping module 103 is provided with a conical opening 1010 which is shaped to match the conical liquid suction head 1022, so as to facilitate the snap-fit connection of the wiping module 103 and the liquid suction module 102.

[0052] As an optional implementation, the flexible wiping part 1032 is provided in the shape of a conical or cylindrical sponge.

[0053] Specifically, as shown in Figure 6 , in order to adaptively clean the hole sites 2011 of the deep-well plate 201, the flexible wiping part 1032 is provided in the shape of a conical or cylindrical sponge, so as to facilitate the cleaning of the hole sites 2011.

[0054] As an optional implementation, the liquid suction head 1022 of the liquid suction module 102 is provided in a plurality, and the conical openings 1010 on the wiping module 103 are provided in one-to-one correspondence with the liquid suction heads 1022.

[0055] Specifically, as shown in Figure 6 , the hole sites of the device to be cleaned, such as the deep-well plate 201, are arranged in an array, so as to facilitate the cleaning of the hole sites on the deep-well plate 201 by providing the wiping parts and the liquid suction heads 1022 corresponding to the hole sites of the deep-well plate 201, which can simultaneously clean or remove the condensed water on the hole sites of the deep-well plate 201, so as to make the cleaning simple and convenient; further, the liquid suction heads 1022 and the wiping parts can be arranged corresponding to the hole sites of different devices to be cleaned.

[0056] According to the above technical solution, the method of use can be as follows:

[0057] (1) Remove the condensed water: remove the wiping module 103, press the top button 10121, align the device with the condensation module 202, and release the top button 10121 to suck out the condensed water in the condensation module 202.

[0058] (2) Module cleaning: A. Dry wiping: align the device with the hole site and slide up and down for wiping; B. Wet wiping 1: remove the wiping module 103 and the liquid suction module 102, press and release the top button 10121 to suck 75% alcohol, install the liquid suction module 102 and the wiping module 103, press the top button 10121 to soak the sponge, and slide up and down for wiping; C. Wet wiping 2: remove the wiping module 103 and the liquid suction module 102, press and release the top button 10121 to suck 75% alcohol, install the liquid suction module 102, press the top button 10121 to inject liquid into the hole, install the wiping module 103, and slide up and down for wiping.

[0059] The technical scheme of the present application has the following beneficial effects:

[0060] 1、 The technical scheme has simple structure, convenient use and low cost.

[0061] 2、 The technical scheme can be used for wiping hole position and removing condensate in hole.

[0062] 3、 The technical scheme can select various wiping modes, such as dry wiping, soaking wiping and wet wiping.

[0063] In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0064] In the description of the present application, it should be noted that unless otherwise specified and limited, the terms "mounting", "connecting", "connecting" should be understood broadly, for example, it can be fixed connection, or detachable connection, or integral connection; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through intermediate medium, or the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0065] The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of changes or replacements within the technical scope disclosed by the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A device for decondensation and cleaning, characterized in that, The application relates to a pipette, which comprises a column body, a pipetting component, a suction module and a wiping module. The pipetting component comprises a piston and a spring, and the spring is arranged between the piston and the column body to keep the piston in an initial position.

2. The apparatus for removing condensate and cleaning of claim 1, wherein, The piston is flush with the upper end of the column body, and a button opening is arranged on the side of the upper end of the column body.

3. The apparatus for decondensing water and cleaning according to claim 2, wherein, The lower end of the column body is provided with a suction column.

4. The apparatus for decondensing water and cleaning according to claim 3, wherein, The outer side of the suction column is provided with an external thread, and the suction module is connected with the column body through the external thread.

5. The apparatus for decondensing water and cleaning according to claim 4, wherein, The suction head is conical.

6. The apparatus for decondensing water and cleaning according to claim 5, wherein, The tip of the suction head is provided with a cross-shaped opening.

7. The apparatus for removing condensate and cleaning of claim 6, wherein, The upper side of the wiping module is provided with a conical opening which is matched with the conical suction head.

8. The apparatus for decondensing water and cleaning according to claim 7, wherein, The flexible wiping part is provided with a conical or columnar sponge.

9. The apparatus for decondensing water and cleaning according to claim 8, wherein, The suction head of the suction module is provided in a plurality of forms, and the conical openings on the wiping module are arranged in one-to-one correspondence with the suction heads.

10. The apparatus for removing condensate and cleaning according to any one of claims 1-9, wherein, ​

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