Simple and automatic exosome purification device

Abstract

The utility model relates to a simple automatic exosome purification device which comprises an installation bottom plate, the installation bottom plate is connected with a purification module, the purification module comprises a fixed shell and a purification chip arranged in the fixed shell, and the two side faces of the fixed shell are respectively provided with a through hole; the vibration module comprises two groups of vibrators which are respectively arranged at the positions of the through holes in the two sides of the fixed shell, and the vibrators are configured to be capable of moving in the direction close to or away from the fixed shell; the liquid level detection module comprises a light source emitting mechanism and a light source receiving mechanism which are connected to the two side faces of the fixed shell correspondingly. By integrating the purification module, the vibration module, the liquid level detection module and the sample adding module, multi-station operation can be realized in a limited space, and the device is compact in overall layout, high in space utilization rate, capable of carrying a core module of the whole purification operation, low in cost and suitable for miniaturization and simplification design of equipment.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical equipment technical field, concretely is a simple automatic purification device of exosome. BACKGROUND

[0002] The separation and purification of exosome mostly adopt a new separation method based on ultrasonic nanofiltration, which applies negative pressure oscillation and double-coupled ultrasonic oscillation system to nanometer ultrafiltration chip, removes and intercepts EVs in the sample through nanometer hole, realizes the enrichment and purification of EVs. The full-automatic exosome extraction system EXODUS can realize the rapid separation of exosome of various biological samples, including but not limited to cell supernatant, blood, urine, saliva, etc., and meanwhile, the separated exosome has high purity, high yield and high biological activity, and the separated exosome can be widely used in proteomics, genomics and metabolomics research.

[0003] At present, the extraction system adopts an independently designed sample adding movement module, the control is complicated, the structure layout space requirement is big, and in the liquid level detection structure, a probe with a driving structure is used for detection, which further increases the layout space, and the development and manufacturing cost is high. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a simple automatic purification device of exosome, which can at least partially solve the technical problems mentioned in the background.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a simple automatic purification device of exosome, comprising a mounting bottom plate, the mounting bottom plate is connected with: a purification module, comprising a fixed shell and a purification chip arranged in the fixed shell, both sides of the fixed shell are respectively provided with through holes; a vibration module, comprising two groups of vibrators arranged in the through hole positions of both sides of the fixed shell, the vibrator is constructed as being capable of moving towards the direction close to or away from the fixed shell; a liquid level detection module, comprising a light source emitting mechanism and a light source receiving mechanism connected to both sides of the fixed shell.

[0006] In a preferred embodiment, the vibration module further comprises a driving mechanism for driving the vibrator to move, the driving mechanism comprises a lead screw motor, a first sliding block connected to the nut end of the lead screw motor, a second sliding block connected to the main body end of the motor, the first sliding block and the second sliding block are slidingly connected to the mounting bottom plate, and the first sliding block and the second sliding block are respectively connected to the two vibrators through a fixed frame.

[0007] In a preferred embodiment, the mounting bottom plate is further connected with a limiting column at the separation stroke position corresponding to the first sliding block and the second sliding block.

[0008] In a preferred embodiment, the driving mechanism further comprises a light coupling connected to the first sliding block, and a baffle connected to the second sliding block, the baffle and the light coupling cooperate to achieve a preset distance between the first sliding block and the second sliding block.

[0009] In a preferred embodiment, the light source emitting mechanism comprises a first fixed plate connected to one side surface of the fixed shell, and a light source emitter connected through the first fixed plate, and the light source receiving mechanism comprises a second fixed plate connected to another side surface of the fixed shell, and a light source receiver connected through the second fixed plate.

[0010] In a preferred embodiment, the light source emitter and the light source receiver are arranged in several groups along the height direction of the corresponding first fixed plate or second fixed plate.

[0011] In a preferred embodiment, the purification chip comprises a purification shell made of transparent material.

[0012] In a preferred embodiment, the automatic purification device further comprises a sample adding module, the sample adding module comprises a sample adding needle, the sample adding needle is connected to the first sliding block or the second sliding block through a support, and the sample adding needle corresponds to the position of the purification chip when the vibrator is in a closed state.

[0013] In a preferred embodiment, the sample adding module further comprises a collector connected to the side end surface of the fixed shell, and the collector is arranged on the corresponding side of the sample adding needle.

[0014] Compared with the prior art, the beneficial effects of the automatic purification device for exosomes are as follows: the automatic purification device for exosomes integrates a purification module, a vibration module, a liquid level detection module, and a sample adding module, and can realize multi-station operation in a limited space. The sample adding module and the vibration module use the same driving structure, which not only reduces the space occupation, but also simplifies the control and reduces the motion failure. The liquid level detection module integrated in the purification module can realize real-time feedback of the liquid surface position, and the sample adding module can realize continuous liquid supply. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 FIG. 1 is a first angle structural schematic view of the automatic purification device for exosomes in the embodiment of the present application;

[0016] Figure 2 FIG. 2 is a second angle structural schematic view of the automatic purification device for exosomes in the embodiment of the present application;

[0017] Figure 3 FIG. 3 is an exploded view of the purification module and the liquid level detection module in the embodiment of the present application;

[0018] Figure 4 It is the exploded view of the vibration module in the embodiment of the utility model;

[0019] Figure 5 It is the structure schematic view of the sample adding module in the embodiment of the utility model.

[0020] The meaning of each reference numeral in the figure is as follows:

[0021] 1, installation base plate; 11, positioning pin hole; 2, purification module; 21, fixed shell; 211, through hole; 22, purification shell; 24, internal cavity of retentate; 25, filtrate outlet; 3, liquid level detection module; 31, first fixed plate; 32, light source transmitter; 33, second fixed plate; 34, light source receiver; 4, vibration module; 41, vibrator; 42, screw motor; 43, first sliding block; 44, second sliding block; 45, slide rail; 46, fixed frame; 47, optocoupler; 48, baffle; 49, flexible fixator; 50, guide rail pair; 6, sample adding module; 61, sample adding needle; 62, support; 63, collector; 631, washing liquid inlet; 632, waste liquid outlet. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0023] In the description of the utility model, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like is the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the utility model.

[0024] Referring to Figures 1-2 The embodiment discloses a simple automatic exosome purification device, which comprises an installation base plate 1, the installation base plate 1 is connected with a purification module 2, a vibration module 4, a liquid level detection module 3 and a sample adding module 6. Wherein the vibration module 4 is movably connected to the installation base plate 1 and is configured to act the vibration force on the purification module 2 by moving, the liquid level detection module 3 is connected to the purification module 2 and is used for liquid level detection, and the sample adding module 6 is used for automatic sample adding.

[0025] Specifically, in combination with Figure 3 , the purification module 2 comprises a fixed shell 21 fixedly connected to the installation base plate 1, and the fixed shell 21 is enclosed to form a fixed cavity structure with an opening at the top end. The purification module 2 further comprises a purification chip connected in the fixed cavity. The purification chip and the fixed shell 21 are roughly matched in shape, so that they can be vertically arranged in the fixed cavity. The purification chip comprises a purification shell 22. In this embodiment, the purification shell 22 is configured as a shell with a filter membrane, which encloses to form a trapped liquid cavity 24, i.e. a purification cavity. The purification chip can be powered by an external negative pressure device. Through the negative pressure effect, long-time filtration, purification and concentration of biological samples of different particle sizes in the purification cavity can be realized. The bottom of the cavity is provided with a filtrate outlet 25. The purification chip in this embodiment is a prior art.

[0026] Further, the two side surfaces of the fixed shell 21 are respectively provided with through holes 211, which are used to match the vibration module 4, so that the vibration force generated by the vibration module 4 can directly act on the purification shell 22. The matching structure will be described in detail below.

[0027] Again referring to Figure 3 , the liquid level detection module 3 comprises a light source emitting mechanism and a light source receiving mechanism fixedly connected to the two side surfaces of the fixed shell 21, respectively. Correspondingly, in this embodiment, the purification shell 22 is made of transparent material which is easy to transmit light. The light source emitting mechanism emits light source signals, which successively transmit through the proximal transparent purification shell 22, the liquid or air or mixture in the cavity, the distal transparent purification shell 22 and the light source receiving mechanism, and identify the signal changes of different media in the cavity, so as to determine whether it is liquid or air.

[0028] Specifically, the light source emitting mechanism comprises a first fixed plate 31 fixedly connected to one side surface of the fixed shell 21 along the height direction and a plurality of light source emitters 32 connected through the first fixed plate 31. The light source emitters 32 are distributed at different height positions of the first fixed plate 31. Correspondingly, the fixed shell 21 is provided with a transmission hole at the position corresponding to the light source emitters 32, so that the light source can irradiate to the transparent purification shell 22. Similarly, the light source receiving mechanism comprises a second fixed plate 33 fixedly connected to the other side surface of the fixed shell 21 along the height direction and a plurality of light source receivers 34 connected through the second fixed plate 33. The light source receivers 34 are distributed at different height positions of the second fixed plate 33 and consistent with the height of the light source emitters 32. Correspondingly, the fixed shell 21 is provided with a transmission hole at the position corresponding to the light source receivers 34. By arranging the light source emitters 32 and the light source receivers 34 at different heights, the liquid level of the sample or liquid in the purification cavity can be detected to determine the timing of sample addition or termination of sample addition.

[0029] The embodiment can realize effective detection of the liquid level in the cavity, simplify the device structure and reduce the space occupation by adopting the liquid level detection structure directly connected to the fixed shell 21, and can simplify the operation and be more convenient to control by adopting the light source emission and receiving detection technology compared with the traditional driving probe structure.

[0030] In combination Figure 4 The vibration module 4 includes two groups of vibrators 41 arranged on both sides of the fixed shell 21, and the two vibrators 41 correspond to the positions of the through holes 211 of the fixed shell 21, respectively. The two vibrators 41 are configured to move towards or away from the fixed shell 21. When the vibrator 41 moves to the position of the through hole 211, it abuts on the purification shell 22 by passing through the through hole 211, so as to realize sample separation and purification in the purification cavity by oscillation. For example, the vibrator 41 can adopt an ultrasonic transducer, a vibration motor or other vibration structure, and the embodiment is not limited in particular.

[0031] Further, the vibration module 4 further includes a driving mechanism for driving the vibrator 41 to move. Specifically, the driving mechanism includes a lead screw motor 42, a first sliding block 43 fixedly connected to the nut end of the lead screw motor 42, and a second sliding block 44 fixedly connected to the main body end of the motor. The lead screw motor 42 is fixed by the second sliding block 44, and the first sliding block 43 and the second sliding block 44 are slidably connected to the installation bottom plate 1 by a slide rail 45. The first sliding block 43 and the second sliding block 44 are connected to the two vibrators 41 by a fixing frame 46, respectively. Thus, the two vibrators 41 can be driven to move by the lead screw motor 42.

[0032] It should be noted that the installation bottom plate 1 is further provided with a positioning pin hole 11 for installing a limiting column (not shown in the figure) at a position corresponding to the separation stroke of the two sliding blocks. When it is necessary to separate the vibrators 41, the lead screw motor 42 is started to drive the first sliding block 43 to move away from the fixed shell 21 until it reaches the position of the limiting column, which blocks it. At this time, the second sliding block 44 and the lead screw motor 42 move away from the fixed shell 21 until they reach the position of the other limiting column. Similarly, when it is necessary to close the vibrators 41, the reverse operation can be performed. This structure enables the two vibrators 41 to alternately abut on the purification shell 22.

[0033] In combination Figure 4The first sliding block 43 and the second sliding block 44 are connected to the fixed frame 46 through flexible fixers 49 arranged at lower ends of the first sliding block 43 and the second sliding block 44, respectively. The flexible fixers 49 are used to flexibly connect the first sliding block 43 and the second sliding block 44 to the corresponding fixed frame 46. For example, the flexible fixer 49 can be an elastic member such as a spring or a tension spring, or an elastic structure such as a springy glue. In the embodiment, the driving mechanism further includes an optical coupler 47 fixedly connected to a top end of the first sliding block 43, and a baffle 48 fixedly connected to a top end of the second sliding block 44. The baffle 48 and the optical coupler 47 cooperate to achieve a preset distance between the first sliding block 43 and the second sliding block 44. The driving mechanism of the embodiment can automatically adjust the positions of the first sliding block 43 and the second sliding block 44, so that the distance between the two side vibrators 41 and the fixed shell 21 is finally equal, and under the deformation of the two flexible fixers 49, the same elastic pressure on both sides of the purification shell 22 is achieved.

[0034] To ensure the stability of the movement of the vibrator 41 and the fixed frame 46, the first sliding block 43 and the second sliding block 44 are connected to the corresponding fixed frame 46 through a guide rail pair 50.

[0035] In combination with Figure 5 The sample adding module 6 includes a sample adding needle 61 fixedly connected to a top end of the first sliding block 43 or the second sliding block 44 through a support 62. One end of the sample adding needle 61 is connected to an external sample supply structure such as a syringe or a pump, and the other end corresponds to a top end of the purification cavity in the closed state of the vibrator 41, and is used to inject a sample or a liquid into the purification cavity. The sample adding module 6 further includes a collector 63 fixedly connected to a side end face of the fixed shell 21. The collector 63 is arranged at a corresponding side of the sample adding needle 61, and has a washing liquid inlet 631 and a waste liquid outlet 632 arranged therein. The collector 63 cooperates with the back-and-forth movement of the sample adding needle 61 to realize the cleaning of the sample adding needle 61 and reduce or prevent pollution.

[0036] It should be noted that when the vibrator 41 is in the closed position, the sample adding needle 61 corresponds to the purification cavity, and when the vibrator 41 is in the open position, the sample adding needle 61 corresponds to the collector 63.

[0037] It can be understood that the automatic purification device provided in the embodiment further includes a control module. Each action process mentioned in the embodiment is controlled by the control module. The control module can specifically include a control chip or a single-chip microcomputer which can control multiple modules according to a predetermined program, so that each module can be started, stopped or subjected to other actions according to a preset process.

[0038] The simple automatic purification device for exosomes provided by the embodiment can realize multi-station operation in limited space by integrating the purification module 2, the vibration module 4, the liquid level detection module 3 and the sample adding module 6. The sample adding module 6 and the vibration module 4 use the same driving structure, which not only reduces the space occupation, but also simplifies the control and effectively reduces the motion failure; and the liquid level detection module 3 integrated in the purification module 2 can realize real-time feedback of the liquid surface position, and the sample adding module 6 can realize continuous liquid supply.

[0039] The device has compact overall layout, high space utilization rate, can realize loading of core modules of the whole purification operation, small cost, and is suitable for miniaturization and simple design of the equipment.

[0040] The basic principle, main features and advantages of the utility model are shown and described above. It should be understood by those skilled in the art that the utility model is not limited by the above embodiments, the above embodiments and the description in the specification are only preferred examples of the utility model, and are not used to limit the utility model, various changes and improvements of the utility model can be made without departing from the spirit and scope of the utility model, and these changes and improvements all fall within the scope of the utility model claimed. The scope of protection of the utility model is defined by the appended claims and their equivalents.

Claims

1. An exosome simple automatic purification device, characterized by, The installation base plate (1) is connected with: A purification module (2) comprising a fixed shell (21) and a purification chip arranged in the fixed shell (21), and the fixed shell (21) is provided with a through hole (211) on each side; A vibration module (4) comprising two groups of vibrators (41) arranged at the positions of the through holes (211) on the two sides of the fixed shell (21), and the vibrators (41) are configured to move towards or away from the fixed shell (21); A liquid level detection module (3) comprising a light source emitting mechanism and a light source receiving mechanism connected to the two side surfaces of the fixed shell (21).

2. The exosome simple and automatic purification device according to claim 1, characterized by, The vibration module (4) further comprises a driving mechanism for driving the movement of the vibrators (41), and the driving mechanism comprises a lead screw motor (42), a first sliding block (43) connected to the nut end of the lead screw motor (42), and a second sliding block (44) connected to the motor body end, the first sliding block (43) and the second sliding block (44) are slidingly connected to the installation base plate (1), and the first sliding block (43) and the second sliding block (44) are respectively connected to the two vibrators (41).

3. The exosome simple and automatic purification device according to claim 2, characterized by, The installation base plate (1) is further connected to a limit post at a position corresponding to the separation stroke of the first sliding block (43) and the second sliding block (44).

4. The exosome simple and automatic purification device according to claim 2, characterized by, The driving mechanism further comprises an optocoupler (47) connected to the first sliding block (43) and a baffle (48) connected to the second sliding block (44), and the baffle (48) and the optocoupler (47) cooperate to achieve a predetermined distance between the first sliding block and the second sliding block.

5. The exosome simple and automatic purification device according to claim 1, characterized by, The light source emitting mechanism comprises a first fixed plate (31) connected to one side surface of the fixed shell (21) and a light source emitter (32) connected through the first fixed plate (31), and the light source receiving mechanism comprises a second fixed plate (33) connected to the other side surface of the fixed shell (21) and a light source receiver (34) connected through the second fixed plate (33).

6. The exosome simple and automatic purification device according to claim 5, characterized by, The light source emitter (32) and the light source receiver (34) are arranged in several groups and along the height direction of the corresponding first fixed plate (31) or second fixed plate (33).

7. The exosome simple and automatic purification device according to claim 1, characterized by, The purification chip comprises a purification shell (22) made of transparent material.

8. The exosome simple and automatic purification device according to claim 2, characterized by, The automatic purification device further comprises a sample adding module (6), and the sample adding module (6) comprises a sample adding needle (61) connected to the first sliding block (43) or the second sliding block (44) through a support (62), and the sample adding needle (61) corresponds to the position of the purification chip when the vibrator (41) is closed.

9. The exosome simple and automatic purification device according to claim 8, characterized by, The sample adding module (6) further comprises a collector (63) connected to the side end surface of the fixed shell (21), and the collector (63) is arranged at the corresponding side of the sample adding needle (61).

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