Micro-tissue gas-liquid interface culture device

Abstract

According to the micro-tissue gas-liquid interface culture device provided by the invention, the driving shafts are driven to rotate through the driving motor, so that the culture spinner bottle positioned between the two driving shafts is driven to rotate, and the rotation of the culture spinner bottle is realized. The rotation of the culture spinner bottle enables the micro-tissue to continuously receive fresh gas and a culture medium at a gas-liquid interface, a gas-phase culture period and a liquid-phase culture period are provided, and the two periods are sequentially carried out, so that in-vivo alleviation of the gas-liquid interface is effectively simulated, the survival and growth of the micro-tissue are promoted, and the culture efficiency is improved. The device is compact in structure and easy and convenient to operate, and the operation difficulty and labor intensity of experimenters are reduced. The device is wide in application range, can be suitable for various cell types, and can also be used in the fields of scientific research experiments, biotechnology, medical diagnosis and the like.

Description

Technical Field

[0001] This invention belongs to the field of cell biology device technology and relates to a micro-tissue gas-liquid interface culture device. Background Technology

[0002] Microtissue culture technology is crucial in biomedical research and drug development. Current microtissue culture methods typically include streak plating, spread plating, pour plating, and liquid culture. However, these methods often struggle to precisely control the gas-liquid interface conditions, affecting cell growth and differentiation. Furthermore, while rotating culture devices in existing microtissue culture techniques can improve the culture environment to some extent, they still suffer from low culture efficiency and operational complexity. Utility Model Content

[0003] The purpose of this invention is to provide a micro-tissue gas-liquid interface culture device to solve the problem of low culture efficiency in existing culture devices.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] This utility model provides a micro-tissue gas-liquid interface culture device, including a base and a housing fastened to the base. The base is provided with a drive motor and two drive shafts that are connected by transmission. Both drive shafts are exposed outside the housing, and a culture flask is arranged in close contact between the two drive shafts.

[0006] Preferably, the drive motor is provided with a transmission wheel, the drive shaft is provided with a roller, and the transmission wheel is connected to the roller in a transmission connection.

[0007] Preferably, the culture flask includes a flask body, a breathable cap connected to the flask body, and a tissue growth adhesion layer disposed inside the flask body.

[0008] Preferably, the breathable bottle cap is provided with a breathable membrane.

[0009] Preferably, the tissue growth adhesion layer has micropores.

[0010] Preferably, the tissue growth adhesion layer is annular in shape.

[0011] Preferably, the base is further provided with a control circuit board, which is electrically connected to the drive motor.

[0012] Preferably, the housing is provided with a touch control screen, which is electrically connected to the control circuit board.

[0013] This utility model has the following beneficial effects:

[0014] (1) In this application, the drive motor drives the drive shaft to rotate, which in turn drives the culture flask located between the two drive shafts to rotate, thereby realizing the rotation of the culture flask. This allows the micro-tissue to continuously receive fresh gas and culture medium at the gas-liquid interface, with both gas phase culture period and liquid phase culture period, which proceed sequentially. This effectively simulates the in vivo relief of the gas-liquid interface, promotes the survival and growth of micro-tissue, and improves the culture efficiency.

[0015] (2) The setting of the tissue growth adhesion layer ensures the stability of the gas-liquid interface and avoids the influence of gas-liquid interface fluctuations on cell growth in traditional culture methods.

[0016] (3) The device has a compact structure and is easy to operate, which reduces the difficulty of operation and labor intensity for experimental personnel.

[0017] (4) The device has a wide range of applications and can be used for various cell types, as well as scientific research experiments, biotechnology and medical diagnosis. Attached Figure Description

[0018] Figure 1 A three-dimensional structural schematic diagram of the micro-tissue gas-liquid interface culture device provided in the embodiments of this application;

[0019] Figure 2 An exploded three-dimensional view of the micro-tissue gas-liquid interface culture device provided in the embodiments of this application;

[0020] Figure 3 A schematic diagram of the external structure of the culture roller bottle provided in the embodiments of this application;

[0021] Figure 4 This is a schematic diagram of the internal structure of the culture roller bottle provided in the embodiments of this application;

[0022] Symbolic representation:

[0023] 1-Base, 2-Casing, 3-Drive motor, 4-Drive shaft, 5-Cultivation flask, 6-Transmission wheel, 7-Roller, 8-Control circuit board, 9-Touch control screen;

[0024] 51-Bottle body, 52-Ventilable bottle cap, 53-Tissue growth adhesion layer, 54-Micropores, 55-Fixing component. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] This application provides a micro-tissue gas-liquid interface culture device, which includes a base 1 and a housing 2 fastened to the base 1, as shown in the attached figure. Figure 1 , 2 As shown. The base 1 is the supporting component of the entire device, and the housing 2 is the shell component of the entire device. When the two are fastened together, they form the external structure of the micro-tissue gas-liquid interface culture device.

[0027] The base 1 is equipped with a drive motor 3 and two drive shafts 4, which are connected in a transmission manner. Thus, the drive motor 3 can drive the two drive shafts 4 to rotate. In this embodiment, the drive motor 3 is located inside the housing 2, while the two drive shafts 4 are exposed outside the housing 2, and a culture flask 5 is positioned in close contact between the two drive shafts 4. Therefore, when the drive shafts 4 rotate, the culture flask 5, which is in close contact with the drive shafts 4, can be driven to rotate, thereby allowing the micro-tissues to continuously receive fresh gas and culture medium at the gas-liquid interface. This provides both gas-phase and liquid-phase culture periods, which proceed sequentially, effectively simulating in vivo remission at the gas-liquid interface and promoting the survival and growth of the micro-tissues.

[0028] In this embodiment, the drive motor 3 is equipped with a transmission wheel 6, and the drive shaft 4 is equipped with a roller 7. The transmission wheel 6 and the roller 7 are connected by a transmission mechanism, thereby connecting the drive motor 3 and the two drive shafts 4 to ensure the smooth rotation of the culture flask 5. Furthermore, the rotation speed of the drive motor 3 can be adjusted according to the characteristics of different tissues and cells, thereby adjusting the rotation speed of the culture flask 5 and achieving rotation-stop intervals.

[0029] In addition, a control circuit board 8 is provided on the base 1. This control circuit board 8 is electrically connected to the drive motor 3, so as to control the rotation of the drive motor 3 and adjust its speed through the control circuit board 8. For displaying and manually adjusting the speed of the drive motor 3, a touch control screen 9 is provided on the housing 2, and the touch control screen 9 is electrically connected to the control circuit board 8.

[0030] In this embodiment, the culture roller bottle 5 includes a bottle body 51, a breathable cap 52, and a tissue growth adhesion layer 53, as shown in the attached figure. Figure 3 , 4 As shown, the bottle body 51 is connected to the vent cap 52, and the bottle body 51 is tightly placed between the two drive shafts 4. The bottle body 51 is made of transparent material to facilitate observation of micro-tissue growth. Culture medium and micro-tissue samples can be added into the bottle body 51 through the vent cap 52. In addition, the vent cap 52 is equipped with a vent membrane, which is made of a membrane that allows only gas exchange to achieve gas exchange inside the bottle body 51, facilitating micro-tissue growth.

[0031] The tissue growth adhesion layer 53 has micropores 54 to facilitate micro-tissue adhesion and growth. Simultaneously, the tissue growth adhesion layer 53 ensures the stability of the gas-liquid interface, avoiding the impact of gas-liquid interface fluctuations on cell growth in traditional culture methods. To accommodate the rotation of the bottle 51, the tissue growth adhesion layer 53 is annular in shape. The tissue growth adhesion layer 53 is fixed to the inner wall of the bottle 51 by a fastener 55.

[0032] The micro-tissue gas-liquid interface culture device provided in this embodiment is used as follows: Open the vent cap 52, add the culture medium and micro-tissue sample into the bottle body 51, and tighten the vent cap 52. Set the speed of the drive motor 3 and start the drive motor 3 via the touch control screen 9. The start of the drive motor 3 causes the transmission wheel 6 to rotate, which in turn drives the drive shaft 4 to rotate via the roller 7. The rotation of the drive shaft 4 drives the culture rotating bottle 5 to rotate, realizing the culture of micro-tissues at the gas-liquid interface.

[0033] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A microtissue gas-liquid interface culture device, characterized by, The application relates to a culture device, which comprises a base (1) and a casing (2) buckled on the base (1), the base (1) is provided with a driving motor (3) and two driving shafts (4) in transmission connection, the two driving shafts (4) are exposed to the casing (2), and a culture rotating bottle (5) is arranged in close contact between the two driving shafts (4).

2. The microtissue gas-liquid interface culture device according to claim 1, wherein, The driving motor (3) is provided with a transmission wheel (6), the driving shaft (4) is provided with a rolling wheel (7), and the transmission wheel (6) is in transmission connection with the rolling wheel (7).

3. The microtissue gas-liquid interface culture device according to claim 1, wherein, The culture rotating bottle (5) comprises a bottle body (51), a gas-permeable bottle cover (52) connected with the bottle body (51) and a tissue growth adhesive layer (53) arranged in the bottle body (51).

4. The microtissue gas-liquid interface culture device according to claim 3, wherein, The gas-permeable bottle cover (52) is provided with a gas-permeable film.

5. The microtissue gas-liquid interface culture device according to claim 3, wherein, The tissue growth adhesive layer (53) is provided with micropores (54).

6. The microtissue gas-liquid interface culture device according to claim 3, wherein, The tissue growth adhesive layer (53) is in the shape of a circular ring.

7. The microtissue gas-liquid interface culture device according to any one of claims 1 to 6, wherein The base (1) is further provided with a control circuit board (8), and the control circuit board (8) is electrically connected with the driving motor (3).

8. The microtissue gas-liquid interface culture device according to claim 7, wherein, The casing (2) is provided with a touch control screen (9), and the touch control screen (9) is electrically connected with the control circuit board (8).

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