Efficient ultrafiltration equipment for polypeptide production

Abstract

The utility model relates to the technical field of polypeptide ultrafiltration production, and discloses efficient ultrafiltration equipment for polypeptide production, which comprises a bracket, a top block, a bottom block, a top plate and a bottom plate, the concentrated solution treatment assembly comprises a booster pump and an ultrafiltration assembly, the booster pump is arranged on one side of the support and used for pressurizing a polypeptide solution, the ultrafiltration assembly comprises an ultrafiltration barrel, and the ultrafiltration barrel is arranged in the support and used for ultrafiltration shunting; the collecting assembly comprises a concentrated solution collecting box I and a concentrated solution collecting box II which are arranged on the bracket and are used for collecting a polypeptide concentrated solution; the stirring anti-blocking assembly comprises a plurality of groups of stirring blades arranged in the ultrafiltration barrel and used for stirring the polypeptide concentrated solution, and the driving assembly is arranged at the top of the bracket, comprises a motor and an incomplete gear and is used for driving the stirring blades. According to the utility model, the ultrafiltration can be effectively carried out, the blockage in the ultrafiltration process can be prevented, the working efficiency is improved, and the maintenance cost is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to polypeptide ultrafiltration production technical field, concretely is a kind of efficient polypeptide production with ultrafiltration equipment. BACKGROUND

[0002] Polypeptide ultrafiltration production is a kind of production process using ultrafiltration technology to separate, purify and concentrate polypeptide.Ultrafiltration is one of membrane separation technologies driven by pressure.The membrane pore size is between 20-1000A° for the purpose of separating macromolecules from small molecules.Hollow fiber ultrafilter has the advantages of high packing density in unit container and small floor area.Ultrafiltration is widely used in polypeptide production.The existing polypeptide ultrafiltration equipment assembly is precise, and the filtration is easy to block during polypeptide ultrafiltration process, affecting efficiency.

[0003] After searching, the prior art (application number: CN201721563527.0) a kind of polypeptide ultrafiltration device, wherein "the raw material tank is connected with security filter, the security filter is connected with first ultrafiltration pipe, first ultrafiltration pipe is connected with second ultrafiltration pipe;The lower end of the first ultrafiltration pipe is connected with three-way valve, one end of the three-way valve is connected with the lower end of the second ultrafiltration pipe, and the other end of the three-way valve is connected with the first filter storage tank;The first filter storage tank outlet is connected with the second ultrafiltration pipe feed port and the third ultrafiltration pipe feed port;The third ultrafiltration pipe outlet is connected with the second filter storage tank;The bottom of the first ultrafiltration pipe, the second ultrafiltration pipe and the third ultrafiltration pipe is respectively connected with waste liquid tank".The utility model can effectively ultrafiltrate polypeptide solution.

[0004] However, although the prior art can effectively complete ultrafiltration, it still has some deficiencies: easy to block, affecting filtration efficiency. UTILITY MODEL CONTENTS

[0005] The utility model aims at solving the shortcomings in the prior art and provides an efficient polypeptide production ultrafiltration equipment.

[0006] To achieve the above object, the utility model adopts the following technical scheme: an efficient polypeptide production ultrafiltration equipment, comprising: a support, a top block is arranged at the top; a concentrated solution processing assembly, comprising a booster pump and an ultrafiltration assembly, the booster pump is arranged on one side of the support, for pressurizing polypeptide solution, the ultrafiltration assembly comprises an ultrafiltration barrel, and the ultrafiltration barrel is arranged in the support, for ultrafiltration shunt, further comprising a collection assembly, and the collection assembly comprises a concentrated solution collection tank one and a concentrated solution collection tank two arranged on the support, for collecting polypeptide concentrate; a stirring anti-blocking assembly, comprising stirring blades arranged in the ultrafiltration barrel, arranged in multiple groups, for stirring polypeptide concentrate, further comprising a driving assembly arranged at the top of the support, the driving assembly comprises a motor and an incomplete gear, for driving the stirring blades.

[0007] As a further description of the above technical solutions:

[0008] The concentrated liquid processing assembly further comprises: a liquid inlet pipe fixedly arranged on the side of the booster pump away from the ultrafiltration barrel, for inputting the polypeptide solution; and a liquid flow pipe one, one end of which is fixedly arranged on the top of the booster pump and the other end of which is fixedly arranged on the ultrafiltration barrel.

[0009] As a further description of the above technical solutions:

[0010] The ultrafiltration assembly comprises: an ultrafiltration net arranged in the ultrafiltration barrel, for filtering the concentrated polypeptide solution; an inner cavity between the ultrafiltration barrel and the ultrafiltration net; a barrel cover arranged on the top of the ultrafiltration barrel; and the liquid flow pipe one penetrating through the barrel cover, for passing the concentrated liquid into the ultrafiltration net.

[0011] As a further description of the above technical solutions:

[0012] The collection assembly comprises: a concentrated liquid collection box one arranged on one side of the support, for collecting the concentrated liquid with large particles; a liquid discharge pipe one, one end of which is fixedly arranged on the bottom of the ultrafiltration net and the other end of which is fixedly arranged on the concentrated liquid collection box one; a concentrated liquid collection box two arranged on the side of the support away from the concentrated liquid collection box one, for collecting the concentrated liquid with small particles; and a liquid discharge pipe two, one end of which is fixedly arranged on the bottom of the ultrafiltration barrel and communicated with the inner cavity and the other end of which is fixedly arranged on the concentrated liquid collection box two.

[0013] As a further description of the above technical solutions:

[0014] The driving assembly comprises: a motor fixedly arranged on the top block; and an incomplete gear fixed to the output end of the motor, for driving the stirring.

[0015] As a further description of the above technical solutions:

[0016] The stirring anti-blocking assembly further comprises: a stirring rod penetrating through the barrel cover and arranged in the ultrafiltration net at the lower end; a gear fixed to the upper end of the stirring rod and engaged with the incomplete gear; a rotation limiting block arranged in the top block and fixed to the upper end of the stirring rod and located above the gear; and stirring blades arranged at the lower end of the stirring rod and arranged in multiple groups, for stirring the concentrated liquid.

[0017] As a further description of the above technical solutions:

[0018] The incomplete gear is engaged with the gear to rotate intermittently, so that the concentrated liquid in the ultrafiltration barrel is prevented from being blocked.

[0019] The utility model has the advantages of:

[0020] 1. The ultrafiltration assembly and the collection assembly are cooperatively operated, the polypeptide concentrated liquid is effectively ultrafiltrated and collected, and the subsequent use is facilitated.

[0021] 2. By working together with the ultrafiltration component and the stirring anti-clogging component, clogging during the ultrafiltration process is avoided, improving work efficiency and reducing maintenance costs. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of an efficient ultrafiltration device for peptide production proposed in this utility model.

[0023] Figure 2 This is a schematic diagram of the concentrate treatment component of an efficient ultrafiltration device for peptide production proposed in this utility model.

[0024] Figure 3 This is a schematic diagram of the drive components of a high-efficiency ultrafiltration device for peptide production proposed in this utility model.

[0025] Figure 4 This is a cross-sectional view of the ultrafiltration component of a high-efficiency ultrafiltration device for peptide production proposed in this utility model.

[0026] Figure 5 This is a schematic diagram of the stirring and anti-clogging component of an ultrafiltration device for peptide production proposed in this utility model.

[0027] Legend: 1. Support; 11. Top block; 2. Concentrate treatment assembly; 201. Liquid inlet pipe; 202. Booster pump; 203. Liquid flow pipe one; 204. Ultrafiltration tank; 205. Ultrafiltration screen; 206. Drain pipe one; 207. Concentrate collection tank one; 208. Drain pipe two; 209. Concentrate collection tank two; 210. Tank lid; 211. Inner cavity; 3. Stirring anti-clogging assembly; 301. Motor; 302. Incomplete gear; 303. Gear; 304. Stirring rod; 305. Stirring blade; 306. Rotation limit block. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] Example 1:

[0030] like Figures 1 to 5As shown, the high-efficiency polypeptide production ultrafiltration equipment provided by the embodiment comprises: a support 1 provided with a top block 11 at the top; a concentrated liquid processing assembly 2 comprising a booster pump 202 and an ultrafiltration assembly, the booster pump 202 being arranged on one side of the support 1 and used for boosting the polypeptide solution, the ultrafiltration assembly comprising an ultrafiltration barrel 204 arranged in the interior of the support 1 and used for ultrafiltration shunting, and further comprising a collecting assembly comprising a concentrated liquid collecting box one 207 and a concentrated liquid collecting box two 209 arranged on the support 1 and used for collecting the polypeptide concentrated liquid; and a stirring anti-blocking assembly 3 comprising stirring blades 305 arranged in the interior of the ultrafiltration barrel 204 and arranged in multiple groups and used for stirring the polypeptide concentrated liquid, and further comprising a driving assembly arranged at the top of the support 1 and comprising a motor 301 and an incomplete gear 302 and used for driving the stirring blades 305.

[0031] In the embodiment, the ultrafiltration assembly, the stirring anti-blocking assembly 3 and the collecting assembly constitute the high-efficiency polypeptide production ultrafiltration equipment involved in the application, and a high-efficiency polypeptide production ultrafiltration equipment is realized.

[0032] In the embodiment, the booster pump 202 is a prior art and is a commonly used known technology in the field, and is only used without improvement, so the connection mode is not described in detail.

[0033] In the embodiment, the polypeptide solution is boosted by the booster pump 202, then enters the ultrafiltration barrel 204 for ultrafiltration treatment, and finally the concentrated liquid after ultrafiltration is collected into the concentrated liquid collecting box one 207 and the concentrated liquid collecting box two 209, so that the polypeptide solution is subjected to ultrafiltration treatment and the concentrated liquid is collected.

[0034] In the embodiment, the stirring blades 305 are arranged in multiple groups, and each group of the stirring blades 305 can be distributed in a radial manner to improve the stirring effect.

[0035] It should be noted that the concentrated liquid is stirred by the stirring blades 305 during the ultrafiltration process, and the motor 301 and the incomplete gear 302 are also used to prevent blockage.

[0036] Specifically, the concentrated liquid processing assembly 2 further comprises: a liquid inlet pipe 201 fixedly arranged on the side of the booster pump 202 away from the ultrafiltration barrel 204 and used for inputting the polypeptide solution; and a liquid flow pipe one 203 fixedly arranged at one end on the top of the booster pump 202 and fixedly arranged at the other end on the ultrafiltration barrel 204.

[0037] In the embodiment, the polypeptide solution enters the booster pump 202 through the liquid inlet pipe 201, is boosted, and is then delivered to the ultrafiltration barrel 204 through the liquid flow pipe one 203, so that the polypeptide solution is inputted and delivered to the ultrafiltration barrel 204.

[0038] Specifically, the ultrafiltration assembly comprises: an ultrafiltration net 205 arranged in the ultrafiltration barrel 204 and used for filtering the concentrated polypeptide solution; an inner cavity 211 located between the ultrafiltration barrel 204 and the ultrafiltration net 205; a barrel cover 210 arranged on the top of the ultrafiltration barrel 204; and a liquid flow pipe one 203 penetrating through the barrel cover 210 and used for introducing the concentrated liquid into the ultrafiltration net 205.

[0039] As a preferred embodiment, the pressurized polypeptide solution enters the ultrafiltration net 205 through the liquid flow pipe one 203, is filtered by the ultrafiltration net 205, and is filtered by the inner cavity 211 between the ultrafiltration net 205 and the ultrafiltration barrel 204 to separate the concentrated liquid of different particle sizes.

[0040] Embodiment 2

[0041] Based on the embodiment 1, in order to collect the polypeptide concentrated liquid after ultrafiltration, the collecting assembly is arranged on the support 1.

[0042] Specifically, the collecting assembly comprises: a concentrated liquid collecting box one 207 arranged on one side of the support 1 and used for collecting the concentrated liquid of large particles; a liquid discharge pipe one 206 fixedly arranged at one end on the bottom of the ultrafiltration net 205 and fixedly arranged at the other end on the concentrated liquid collecting box one 207; a concentrated liquid collecting box two 209 arranged on the support 1 and away from the concentrated liquid collecting box one 207 and used for collecting the concentrated liquid of small particles; and a liquid discharge pipe two 208 fixedly arranged at one end on the bottom of the ultrafiltration barrel 204 and communicated with the inner cavity 211 and fixedly arranged at the other end on the concentrated liquid collecting box two 209.

[0043] It should be noted that the concentrated liquid of large particles after the ultrafiltration net 205 filtering flows into the concentrated liquid collecting box one 207 through the liquid discharge pipe one 206, and the concentrated liquid of small particles in the inner cavity 211 of the ultrafiltration barrel 204 flows into the concentrated liquid collecting box two 209 through the liquid discharge pipe two 208, so that the concentrated liquid of different particle sizes after ultrafiltration is collected in different collecting boxes, which is convenient for subsequent use.

[0044] Specifically, the driving assembly comprises: a motor 301 fixedly arranged on the top block 11; and an incomplete gear 302 fixedly arranged on the output end of the motor 301 and used for driving the stirring.

[0045] As a preferred embodiment, the motor 301 starts to rotate after being connected to the power supply, drives the incomplete gear 302 to rotate, and enables the stirring anti-blocking assembly to normally operate.

[0046] Embodiment 3

[0047] Based on the embodiment 2, in order to prevent the ultrafiltration assembly from being blocked during the ultrafiltration process, the stirring anti-blocking assembly 3 is arranged on the ultrafiltration assembly.

[0048] Specifically, the stirring anti-blocking assembly 3 further comprises: a stirring rod 304 penetrating through the barrel cover 210 and arranged in the ultrafiltration net 205 at the lower end; a gear 303 fixed at the upper end of the stirring rod 304 and engaged with the incomplete gear 302; a rotation limiting block 306 arranged in the top block 11 and fixed at the upper end of the stirring rod 304 and located above the gear 303; and stirring blades 305 arranged at the lower end of the stirring rod 304 and arranged in multiple groups for stirring the concentrated solution.

[0049] It should be noted that the motor 301 drives the incomplete gear 302 to rotate, the incomplete gear 302 is engaged with the gear 303, the stirring rod 304 and the stirring blades 305 are driven to rotate, the rotation limiting block 306 limits the stirring rod 304, and the stirring blades 305 stir the concentrated solution in the ultrafiltration net 205, thereby effectively preventing the concentrated solution in the ultrafiltration barrel 204 from blocking the ultrafiltration net 205 and ensuring the continuous ultrafiltration work.

[0050] Specifically, the incomplete gear 302 is engaged with the gear 303 to rotate intermittently, thereby preventing the concentrated solution in the ultrafiltration barrel 204 from being blocked.

[0051] In this embodiment, the intermittent rotation of the incomplete gear 302 drives the gear 303 to rotate intermittently, and further drives the stirring rod 304 and the stirring blades 305 to rotate intermittently, so that the concentrated solution can be stirred by intermittent rotation, the problem of blocking caused by continuous stirring of the concentrated solution can be avoided, and the anti-blocking purpose is achieved.

[0052] In use, when the polypeptide solution needs to be ultrafiltrated, the user pours the polypeptide solution from the liquid inlet pipe 201 into the booster pump 202, and then the water pressure of the booster pump 202 flows into the liquid flow pipe one 203, and then into the ultrafiltration barrel 204, and the particles with small size are stored in the inner cavity 211 by the ultrafiltration net 205, and the particles with large size enter the inside of the ultrafiltration net 205, and at the same time, the motor 301 is started, the incomplete gear 302 is engaged with the gear 303 to rotate intermittently, the stirring rod 304 drives the stirring blades 305 to stir the concentrated solution for better filtration. After the separation, the particles with small size enter the liquid outlet pipe two 208 from the bottom hole of the inner cavity 211 and finally reach the concentrated solution collecting box two 209 for storage, and the particles with large size enter the liquid outlet pipe one 206 from the bottom hole of the ultrafiltration barrel 204 and the ultrafiltration net 205 and finally reach the concentrated solution collecting box one 207 for storage.

[0053] It should be noted that all the electrical components in this document are connected to the main controller and 220V mains electricity, and the main controller can be a conventional known device controlled by a computer, and the detailed description of the known functions and known components is omitted in the specific embodiment of the disclosure. In order to ensure the compatibility of the device, the operation means adopted is consistent with the parameters of the market appliances.

[0054] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for part of the technical features of the equivalent replacement, the spirit and principles of the present application, made any modification, equivalent replacement, improvement, etc., should be included within the scope of the present application.

Claims

1. An efficient polypeptide production ultrafiltration apparatus, characterized by: The utility model relates to a high -efficient polypeptide production uses ultrafiltration equipment, including: Support (1), top is equipped with top block (11); Concentrate processing assembly (2), including booster pump (202) and ultrafiltration assembly, booster pump (202) is arranged at one side of support (1), is used for polypeptide solution booster, the ultrafiltration assembly includes ultrafiltration barrel (204), and ultrafiltration barrel (204) is arranged in the inside of support (1), is used for ultrafiltration shunt, still including collection assembly, and collection assembly includes the concentrate collection box one (207) and concentrate collection box two (209) being arranged on support (1), is used for collecting polypeptide concentrate; Stirring anti -blocking assembly (3), including the stirring vane (305) being arranged in the inside of ultrafiltration barrel (204), is arranged in multiple groups, is used for stirring polypeptide concentrate, still including drive assembly, is arranged at the top of support (1), and drive assembly includes motor (301) and incomplete gear (302), is used for driving stirring vane (305).

2. The high-efficiency polypeptide production ultrafiltration equipment according to claim 1, wherein: The concentrate processing assembly (2) further comprises: A liquid inlet pipe (201) fixedly arranged on the side of the booster pump (202) away from the ultrafiltration barrel (204) for inputting the polypeptide solution; A liquid flow pipe one (203) fixedly arranged at one end on the top of the booster pump (202) and at the other end on the ultrafiltration barrel (204).

3. The high-efficiency polypeptide production ultrafiltration equipment according to claim 2, wherein: The ultrafiltration assembly comprises: An ultrafiltration mesh (205) arranged in the ultrafiltration barrel (204) for filtering the concentrated polypeptide solution; An inner cavity (211) between the ultrafiltration barrel (204) and the ultrafiltration mesh (205); A barrel cover (210) arranged on the top of the ultrafiltration barrel (204); The liquid flow pipe one (203) penetrates through the barrel cover (210) for passing the concentrated liquid into the ultrafiltration mesh (205).

4. The high-efficiency polypeptide production ultrafiltration equipment according to claim 3, wherein: The collection assembly comprises: The concentrate collection box one (207) arranged on one side of the support (1) for collecting the concentrated liquid with large particles; A liquid discharge pipe one (206) fixedly arranged at one end on the bottom of the ultrafiltration mesh (205) and at the other end on the concentrate collection box one (207); The concentrate collection box two (209) arranged on the side of the support (1) away from the concentrate collection box one (207) for collecting the concentrated liquid with small particles; A liquid discharge pipe two (208) fixedly arranged at one end on the bottom of the ultrafiltration barrel (204) and at the other end on the concentrate collection box two (209).

5. The high-efficiency polypeptide production ultrafiltration equipment according to claim 4, wherein: The drive assembly comprises: The motor (301) fixedly arranged on the top block (11); The incomplete gear (302) fixedly arranged on the output end of the motor (301) for driving stirring.

6. The high-efficiency polypeptide production ultrafiltration equipment according to claim 5, wherein: The stirring anti-blocking assembly (3) further comprises: A stirring rod (304) penetrates the barrel cover (210) and is arranged in the ultrafiltration net (205) with its lower end; a gear (303) is fixed on the upper end of the stirring rod (304) and engages with the incomplete gear (302); A rotation limiting block (306) is arranged in the top block (11) and is fixed on the upper end of the stirring rod (304) and is located above the gear (303); Stirring blades (305) are arranged on the lower end of the stirring rod (304) and are arranged in multiple groups for stirring the concentrated solution.

7. The efficient ultrafiltration equipment for polypeptide production according to claim 6, characterized in that: The incomplete gear (302) intermittently rotates the gear (303) to prevent the concentrated solution in the ultrafiltration barrel (204) from being blocked.

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