Liquid drop generation and culture device

Abstract

A liquid drop generation and culture device comprises a chip base, a chip cover plate, a coil pipe, a coil pipe base and a coil pipe cover plate, is simple in structure and convenient to install and replace, and can be flexibly configured as a liquid drop generation chip or a liquid drop sorting chip; a liquid drop system in the coil pipe is stable, the coil pipe is convenient to disassemble, the requirements for off-line or on-line culture of liquid drops can be met respectively, and the risks of displacement, fusion, breakage, pollution and the like of the liquid drops in the collecting and transferring process are reduced.

Description

Technical Field

[0001] This utility model relates to the field of droplet microfluidics, specifically a droplet generation and cultivation device. Background Technology

[0002] Microfluidics is a technology that manipulates tiny volumes of liquid based on microfluidic chips. In biochemical experiments, these dispersed microdroplet units are used to replace conventional reactors in the laboratory, enabling miniaturized biochemical reactions and detections. Typically, after generating droplets using microfluidics, the droplets need to be cultured in situ or offline, depending on the experimental requirements. The common practice is to collect the generated droplets in simple plastic or glass containers for cultivation or preservation, and then pump the droplets from the containers into the microfluidic instrument for sorting. This method is complex and suffers from problems such as fragility and contamination during droplet transfer. Utility Model Content

[0003] To address the aforementioned problems, this utility model provides a droplet generation and cultivation device, comprising a chip base, a chip cover plate, a coil, a coil base, and a coil cover plate. The chip base is provided with a pipe groove and a first positioning post. A pipe is placed within the pipe groove. A second positioning post is provided on the chip cover plate. The coil base is provided with a first positioning hole and a protrusion. Several limiting posts are provided along the outer edge of the coil base, and an annular space formed by the limiting posts and the protrusion is used to place the coil. The coil cover plate is provided with a second positioning hole, and the first positioning post, the second positioning post, the first positioning hole, and the second positioning hole are positioned correspondingly. The pipe groove includes a first oil phase pipe groove, a water phase pipe groove, a gas phase pipe groove, a first droplet pipe groove, and a waste liquid pipe groove, which are cross-connected. The pipe groove also includes a second oil phase pipe groove, a second droplet pipe groove, and a sorting pipe groove, which are T-shaped connected.

[0004] Preferably, the coil cover plate is engaged with the coil base.

[0005] Preferably, the chip base and the chip cover are engaged and connected, and small holes are provided at the intersection of the outer edges of the chip base and the chip cover with the channel groove.

[0006] Preferably, a quick-connect fitting is included, through which the coil is connected to the pipe.

[0007] Preferably, a Robert stop clamp is provided on the coil.

[0008] This invention provides a droplet generation and cultivation device with a simple structure, convenient installation and replacement, and can be flexibly configured as a droplet generation chip or a droplet sorting chip. The droplet system inside the coil is stable, and the coil is easy to disassemble. It can meet the needs of offline or online droplet cultivation, and reduce the risks of droplet displacement, fusion, breakage, and contamination during collection and transportation. Attached Figure Description

[0009] The above and / or additional aspects and advantages of this invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0010] Figure 1 This is a schematic diagram of the structure of an embodiment of the present utility model;

[0011] Figure 2 This is a schematic diagram of the chip base structure according to an embodiment of the present invention;

[0012] Figure 3 This is a schematic diagram of the chip cover structure according to an embodiment of the present invention;

[0013] Figure 4 This is a schematic diagram of the coil base structure according to an embodiment of the present invention;

[0014] Figure 5 This is a schematic diagram of the coil cover plate structure according to an embodiment of the present utility model;

[0015] Figure 6 This is a schematic diagram of the coil according to an embodiment of the present invention;

[0016] Figure 7 This is a schematic diagram of a droplet generation chip structure configured according to an embodiment of the present invention;

[0017] Figure 8 This is a schematic diagram of droplet generation according to an embodiment of the present invention;

[0018] Figure 9 This is a schematic diagram of a droplet sorting chip structure configured according to an embodiment of the present invention;

[0019] Figure 10 This is a schematic diagram of droplet sorting according to an embodiment of the present invention. Detailed Implementation

[0020] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0021] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0022] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, an electrical connection, or a connection that allows for communication; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0023] The following disclosure provides many different embodiments or examples for implementing various structures of this invention. To simplify the disclosure, specific examples of components and arrangements are described below. Of course, these are merely examples and are not intended to limit the scope of this invention.

[0024] Please see Figure 1-6 This utility model provides a droplet generation and cultivation device 1, including a chip base 11, a chip cover plate 12, a coil 15, a coil base 13, and a coil cover plate 14. The chip base 11 is provided with a channel groove 112 and a first positioning post 111. The channel groove 112 is used to house the channel. The chip cover plate 12 is provided with a second positioning post 121. The coil base 13 is provided with a first positioning hole 133. The coil base 13 has a protrusion 131. A plurality of limiting posts 132 are provided along the outer edge of the coil base 13 in a direction perpendicular to the plane of the coil base 13. The annular space formed between the limiting posts 132 and the protrusion 131 is used to place the coil 15. The coil cover plate 14 is provided with a second positioning hole 141. The positions of the first positioning post 111, the second positioning post 121, the first positioning hole 133, and the second positioning hole 141 are corresponding.

[0025] The droplet generation and cultivation device 1 is positioned by the first positioning post 111, the second positioning post 121, the first positioning hole 133 and the second positioning hole 141, and the chip base 11, the chip cover plate 12, the coil base 13 and the coil cover plate 14 are stacked and assembled from bottom to top in sequence.

[0026] Pipeline 112 includes a first oil phase pipeline 114, a water phase pipeline 115, a gas phase pipeline 116, a first droplet pipeline 117, and a waste liquid pipeline 118. The first oil phase pipeline 114, water phase pipeline 115, gas phase pipeline 116, and first droplet pipeline 117 are cross-connected. Pipeline 112 also includes a second oil phase pipeline 119, a second droplet pipeline 120, and a sorting pipeline 121. The second oil phase pipeline 119, the second droplet pipeline 120, and the sorting pipeline 121 are T-shaped connected.

[0027] like Figure 7 As shown, in some embodiments, the chip base 11 can be configured as a droplet generating chip. The first oil phase pipeline 1141, the water phase pipeline 1151, the gas phase pipeline 1161, and the first droplet pipeline 1171 are respectively provided in the first oil phase pipeline 114, the water phase pipeline 115, the gas phase pipeline 116, and the first droplet pipeline 117, and their connection outlets are connected by cross joints. The waste liquid pipeline 1181 is provided in the waste liquid pipeline 118, and the two ends of the coil 15 are respectively connected to the first droplet pipeline 1171 and the waste liquid pipeline 1181.

[0028] like Figure 8 As shown, in this mode, oil phase, water phase and gas phase are input through the first oil phase pipeline 1141, water phase pipeline 1151 and gas phase pipeline 1161 respectively, continuously generating water phase-gas phase-oil phase interleaved droplets and entering the coil 15. The uneven droplets generated in the previous stage can be discharged through the waste liquid pipeline 1181.

[0029] like Figure 9 As shown, in some embodiments, the chip base 11 can be configured as a droplet sorting chip. The first oil phase channel 1141, the gas phase channel 116, the first droplet channel 117, the second oil phase channel 119, the second droplet channel 120, and the sorting channel 121 are respectively built into the first oil phase channel 1141, the gas phase channel 1161, the first droplet channel 1171, the second oil phase channel 1191, the second droplet channel 1201, and the sorting channel 1211. The first oil phase channel 1141, the gas phase channel 1161, and the first droplet channel 1171 are connected in a T-shape, and the second oil phase channel 1191, the second droplet channel 1201, and the sorting channel 1211 are connected in a T-shape. The two ends of the coil 15 are respectively connected to the first droplet channel 1171 and the second droplet channel 1201.

[0030] participate Figure 10 In this mode, droplets with oil-gas phase spacing are continuously generated by the first oil phase pipeline 1141 and the gas phase pipeline 1161, which drive the droplets in the coil to move into the second droplet pipeline 1201. At the same time, the second oil phase pipeline 1191 also continuously inputs oil phase to increase the spacing between droplets, which is convenient for downstream detection and sorting.

[0031] The coil cover 14 is preferably engaged with the coil base 13, and the chip base 11 is preferably engaged with the chip cover 12. This connection method is simple in structure and easy to use.

[0032] Furthermore, several small holes 113 are made at the intersection of the chip base 11 and the chip cover 12 with the channel groove to facilitate the passage of the channel.

[0033] In some embodiments, the coil 15 is connected to the pipe via a quick-connect fitting 152, enabling quick insertion and removal; Robert stop clamps 151 are provided at both ends of the coil 15 to control the flow rate. After a certain number of droplets are generated, the Robert stop clamps 151 are clamped to effectively seal the coil 15, ensuring that the droplets inside the coil 15 are in a static state and preventing the droplets from flowing.

[0034] Furthermore, if it is necessary to remove the coil for offline culture, clamp the Robert stop clamp 151, disconnect the quick connector 152, and remove the coil.

Claims

1. A droplet generation and cultivation device, characterized by, Including chip base, chip cover, coil, coil base and coil cover, the chip base is provided with pipeline groove and first positioning column, the pipeline groove is placed in the pipeline, the chip cover is provided with second positioning column; The first positioning hole is arranged on the coil base, the protruding part is arranged on the coil base, a plurality of limiting columns are arranged on the outer edge of the coil base, and an annular space surrounded between the limiting columns and the protruding part is used for placing the coil; The second positioning hole is arranged on the coil cover, the first positioning column, the second positioning column, the first positioning hole and the second positioning hole are corresponding in position; The pipeline groove includes first oil phase pipeline groove, water phase pipeline groove, gas phase pipeline groove, first liquid drop pipeline groove and waste liquid pipeline groove, and the first oil phase pipeline groove, water phase pipeline groove, gas phase pipeline groove and first liquid drop pipeline groove are cross connected; The pipeline groove also includes second oil phase pipeline groove, second liquid drop pipeline groove and sorting pipeline groove, and the second oil phase pipeline groove, second liquid drop pipeline groove and sorting pipeline groove are T-shapedly connected.

2. The droplet generation and culture device of claim 1, wherein The coil cover and the coil base are clamped and connected.

3. The droplet generation and culture device of claim 1, wherein The chip base and the chip cover are clamped and connected, and small holes are arranged at the intersection of the outer edge of the chip base and the chip cover and the pipeline groove.

4. The droplet generation and culture device of claim 1, wherein The coil is connected with the pipeline through the quick connector.

5. The droplet generation and culture device of claim 1, wherein A Robert water stop clamp is arranged on the coil.

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