Gas-phase liquid nitrogen stem cell freezing device

Abstract

The utility model relates to the technical field of biomedical treatment, and discloses a device for freezing stem cells through gas-phase liquid nitrogen. Comprising a box body and further comprises a box cover arranged at the top of the box body, one side of the box body is fixedly connected with a supporting plate, the top of the supporting plate is fixedly connected with a liquid nitrogen tank, the liquid nitrogen tank communicates with a liquid nitrogen pump through a pipeline, and the air outlet end of the liquid nitrogen pump communicates with a mounting pipe; the sealing performance of the box body can be conveniently improved by arranging the sealing mechanism, external air, moisture or pollutants can be effectively prevented from entering the device due to the good sealing performance, so that the purity of liquid nitrogen and the stability of a storage environment are kept, and the box cover can be conveniently and better installed and fixed by arranging the fixing mechanism; the box cover easy to install and fix is usually provided with an accurate matching surface and a sealing structure, and a good sealing effect can be formed after installation.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological medical technology field, concretely is a kind of gas phase liquid nitrogen frozen cell device. BACKGROUND

[0002] Gas phase liquid nitrogen frozen cell device uses gas phase storage technology, makes stem cell suspended in low-temperature nitrogen after liquid nitrogen vaporization, avoids direct contact with liquid nitrogen, the device has intelligent temperature control, monitoring management system, improves storage security and management convenience.

[0003] Gas phase liquid nitrogen frozen cell device carries out liquid nitrogen, sample separation storage mode, stem cell is not directly soaked in liquid nitrogen, therefore, need to use gas phase liquid nitrogen frozen cell device, traditional device can use the sealing material with poor performance in low-temperature environment, such as certain silica gel sealing ring, these materials become hard and brittle under low temperature, prone to crack or failure, to cause sealing performance to decline, poor sealing can cause external air, moisture or other contaminants to enter the device inside, thereby affecting the purity of liquid nitrogen and the stability of storage environment, while traditional liquid nitrogen device can be made of certain specific materials Box cover, these materials can exhibit high hardness and brittleness in low-temperature environment, not easy to assemble accurately, the inconvenience of box cover installation can cause poor sealing performance, if the box cover cannot be tightly attached to the box, liquid nitrogen can leak from the gap, resulting in unstable storage environment, and further affecting the preservation effect of stem cells. SUMMARY

[0004] The utility model aims at providing a kind of gas phase liquid nitrogen frozen cell device, to solve the problems raised in the above background.

[0005] To achieve the above object, the utility model provides the following technical scheme: a kind of gas phase liquid nitrogen frozen cell device, including box, further including:

[0006] Box cover is arranged at the top of the box, one side of the box is fixedly connected with support plate, the top of the support plate is fixedly connected with liquid nitrogen tank, the liquid nitrogen tank is communicated with liquid nitrogen pump by pipeline, the gas outlet end of the liquid nitrogen pump is communicated with installation pipe, the inner wall of the box is installed with storage frame;

[0007] Sealing mechanism for improving the sealing performance of the box is arranged at the top of the box, the sealing mechanism includes notch in the inner wall of the box, the bottom of the box cover is fixedly connected with sealing strip;

[0008] Fixing mechanism for fixing the box cover is arranged at one side of the box, the fixing mechanism includes limiting frame fixed to one side of the box cover, one side of the box cover is provided with buckle plate, the bottom of the buckle plate is fixedly connected with connecting column.

[0009] Preferably, the inner wall of the box cover is fixedly connected with a mounting seat, the inner wall of the mounting seat is rotationally connected with a sleeve rod through a rotating shaft, and the inner wall of the sleeve rod is slidably connected with a sliding rod.

[0010] Preferably, the inner wall of the sliding rod is rotationally connected with a mounting shaft, one end of the mounting shaft is rotationally connected with a connecting disc, and one side of the connecting disc is rotationally connected with the inner wall of the box body.

[0011] Preferably, the inner wall of the box body is rotationally connected with a mounting column for rotating the connecting disc.

[0012] Preferably, one side of the box body is fixedly connected with a fixing frame, one end of the connecting column is fixedly connected with a connecting block, and the inner wall of the connecting block is rotationally connected with a fixing plate through a rotating shaft.

[0013] Preferably, the inner wall of the fixing plate is rotationally connected with a handle through a rotating shaft, and the inner wall of the handle is rotationally connected with a positioning block.

[0014] Preferably, the inner wall of the positioning block is rotationally connected with a damping rotating shaft, and the outer side of the damping rotating shaft is rotationally connected with the inner wall of the fixing frame.

[0015] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0016] The sealing mechanism is arranged, so that the sealing property of the box body is improved, external air, moisture or pollutants are effectively prevented from entering the device, the purity of liquid nitrogen and the stability of the storage environment are maintained, the fixing mechanism is arranged, so that the box cover is better installed and fixed, the box cover which is easy to install and fix is usually designed with accurate matching surfaces and sealing structures, and good sealing effect is formed after installation. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 A structure schematic view of a preferred embodiment of the gas-phase liquid nitrogen cryogenic cell device provided by the utility model is shown in the figure.

[0018] Figure 2 A box body and box cover structure schematic view provided by the utility model is shown in the figure.

[0019] Figure 3 A Figure 1 An enlarged structure schematic view of position A shown in the figure.

[0020] Figure 4 A Figure 2 An enlarged structure schematic view of position B shown in the figure.

[0021] In the figure: 1, box; 2, box cover; 3, support plate; 4, liquid nitrogen tank; 5, liquid nitrogen pump; 6, mounting pipe; 7, sealing mechanism; 71, notch; 72, sealing strip; 8, fixing mechanism; 81, limiting frame; 82, buckle plate; 83, connecting column; 9, mounting seat; 10, sleeve rod; 11, sliding rod; 12, mounting shaft; 13, connecting disc; 14, mounting column; 15, fixing frame; 16, connecting block; 17, fixed plate; 18, handle; 19, positioning block; 20, damping rotating shaft; 21, storage frame. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0023] Please refer to Figures 1-4 As shown in the figure, a kind of gas phase liquid nitrogen frozen cell device, including box 1, by setting box 1, it can be convenient to fix fixing frame 15, the top of box 1 is provided with box cover 2, by setting box cover 2, it can be convenient to fix limiting frame 81, one side of box 1 is fixedly connected with support plate 3, by setting support plate 3, it can be convenient to fix liquid nitrogen tank 4, the top of support plate 3 is fixedly connected with liquid nitrogen tank 4, by setting liquid nitrogen tank 4, it can be convenient to communicate with liquid nitrogen pump 5, liquid nitrogen tank 4 is communicated with liquid nitrogen pump 5 by pipeline, by setting liquid nitrogen pump 5, it can be convenient to communicate with mounting pipe 6, the gas outlet end of liquid nitrogen pump 5 is communicated with mounting pipe 6, by setting mounting pipe 6, it can be convenient to communicate with box 1, the inner wall of box 1 is installed with storage frame 21, by setting storage frame 21, it can be convenient to store stem cell;Sealing mechanism 7 for improving the sealing property of box 1 is set on the top of box 1, sealing mechanism 7 includes notch 71 being set in the inner wall of box 1, by setting notch 71, it can be convenient to connect sealing strip 72, the bottom of box cover 2 is fixedly connected with sealing strip 72, by setting sealing strip 72, it can be convenient to connect box cover 2;Fixing mechanism 8 for fixing box cover 2 is set on one side of box 1, fixing mechanism 8 includes limiting frame 81 being fixed on one side of box cover 2, by setting limiting frame 81, it can be convenient to connect buckle plate 82, one side of box cover 2 is provided with buckle plate 82, by setting buckle plate 82, it can be convenient to connect with connecting column 83, the bottom of buckle plate 82 is fixedly connected with connecting column 83, by setting connecting column 83, it can be convenient to connect with connecting block 16.

[0024] The inner wall of the box cover 2 is fixedly connected with a mounting seat 9. By arranging the mounting seat 9, the sleeve rod 10 can be conveniently rotated. The inner wall of the mounting seat 9 is rotatably connected with the sleeve rod 10 through a rotating shaft. By arranging the sleeve rod 10, the sliding rod 11 can be conveniently slid. The inner wall of the sleeve rod 10 is slidably connected with the sliding rod 11. By arranging the sliding rod 11, the sliding rod 11 can be rotatably connected with the mounting shaft 12. The inner wall of the sliding rod 11 is rotatably connected with the mounting shaft 12. By arranging the mounting shaft 12, the mounting shaft 12 can be rotatably connected with the connecting disc 13. One end of the mounting shaft 12 is rotatably connected with the connecting disc 13. By arranging the connecting disc 13, the mounting shaft 12 can be conveniently rotated. One side of the connecting disc 13 is rotatably connected with the inner wall of the box body 1.

[0025] The inner wall of the box body 1 is rotatably connected with a mounting column 14 for rotating the connecting disc 13.

[0026] One side of the box body 1 is fixedly connected with a fixing frame 15. By arranging the fixing frame 15, the fixing frame 15 can be rotatably connected with the damping rotating shaft 20. Meanwhile, the inner wall of the fixing frame 15 is fixedly connected with a sleeve for sliding the connecting column 83. One end of the connecting column 83 is fixedly connected with a connecting block 16. By arranging the connecting block 16, the connecting block 16 can be rotatably connected with the fixing plate 17 through a rotating shaft. The inner wall of the connecting block 16 is rotatably connected with the fixing plate 17 through a rotating shaft. By arranging the fixing plate 17, the fixing plate 17 can be rotatably connected with the handle 18 through a rotating shaft. The inner wall of the fixing plate 17 is rotatably connected with the handle 18 through a rotating shaft. By arranging the handle 18, the handle 18 can be conveniently lifted. The inner wall of the handle 18 is rotatably connected with the positioning block 19. By arranging the positioning block 19, the positioning block 19 can be rotatably connected with the damping rotating shaft 20. The inner wall of the positioning block 19 is rotatably connected with the damping rotating shaft 20. By arranging the damping rotating shaft 20, the damping rotating shaft 20 can be rotatably connected with the positioning block 19. The outer side of the damping rotating shaft 20 is rotatably connected with the inner wall of the fixing frame 15.

[0027] Working principle: in use, the stem cells are placed on the storage frame 21, and the box cover 2 is covered. The handle 18 is rotated downward around the damping rotating shaft 20. The handle 18 moves downward to drive the fixing plate 17 to move. The fixing plate 17 moves to drive the connecting block 16 to move. The connecting block 16 moves to drive the connecting column 83 to move. The connecting column 83 moves downward to drive the buckle plate 82 to move downward. The slot hole arranged on the buckle plate 82 is clamped to the limiting frame 81. The damping force of the damping rotating shaft 20 avoids the buckle plate 82 from being loosened greatly. Thus, the box cover 2 is installed and fixed. The liquid nitrogen is stored in the liquid nitrogen tank 4. After being pressurized by the liquid nitrogen pump 5, the liquid nitrogen enters the box body 1. The gasification of the liquid nitrogen absorbs heat to cool the box body 1.

[0028] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting; it is not intended to exclude myriad other embodiments of the present application that other inventors can develop based on the same general inventive concepts embodied by the described embodiments. That is, although the present application is described in terms of particular embodiments and implementations, it is to be understood that the terminology used is for the purpose of descriptive clarity and that it is intended to be limited only by the words recited in the appended claims. The scope of the present application shall be limited only by the claims.

[0029] While the embodiments of the present application have been shown and described with respect to particular embodiments thereof, it will be understood by those skilled in the art that various changes in form and details can be made therein without departing from the spirit and scope of the application. Therefore, the scope of the application should not be limited by the embodiments, but should be defined only in accordance with the following claims and their equivalents.

Claims

1. A device for freezing stem cells in gaseous phase with liquid nitrogen, comprising a box (1), characterized in that, Also include: The box (1) is provided at the top of the box cover (2), one side of the box (1) is fixedly connected with the supporting plate (3), the top of the supporting plate (3) is fixedly connected with the liquid nitrogen tank (4), the liquid nitrogen tank (4) is communicated with the liquid nitrogen pump (5) through the pipeline, the gas outlet end of the liquid nitrogen pump (5) is communicated with the installation pipe (6), the inner wall of the box (1) is installed with the storage frame (21); The sealing mechanism (7) is arranged on the top of the box (1) to improve the sealing performance of the box (1), the sealing mechanism (7) comprises a slot (71) formed in the inner wall of the box (1), and the bottom of the box cover (2) is fixedly connected with a sealing strip (72); The fixing mechanism (8) is arranged on one side of the box (1) for fixing the box cover (2), the fixing mechanism (8) comprises a limiting frame (81) fixed on one side of the box cover (2), one side of the box cover (2) is provided with a buckle plate (82), and the bottom of the buckle plate (82) is fixedly connected with a connecting column (83).

2. The apparatus of claim 1, wherein: The inner wall of the box cover (2) is fixedly connected with a mounting seat (9), the inner wall of the mounting seat (9) is rotatably connected with a sleeve rod (10) through a rotating shaft, and the inner wall of the sleeve rod (10) is slidably connected with a sliding rod (11).

3. A device for freezing cells by gaseous phase liquid nitrogen according to claim 2, characterized in that: The inner wall of the sliding rod (11) is rotatably connected with a mounting shaft (12), one end of the mounting shaft (12) is rotatably connected with a connecting disc (13), and one side of the connecting disc (13) is rotatably connected with the inner wall of the box (1).

4. The apparatus of claim 1, wherein: The inner wall of the box (1) is rotatably connected with a mounting column (14) for rotating the connecting disc (13).

5. The apparatus of claim 1, wherein: One side of the box (1) is fixedly connected with a fixing frame (15), one end of the connecting column (83) is fixedly connected with a connecting block (16), the inner wall of the connecting block (16) is rotatably connected with a fixing plate (17) through a rotating shaft.

6. A device for freezing cells by gaseous phase liquid nitrogen according to claim 5, wherein: The inner wall of the fixing plate (17) is rotatably connected with a handle (18) through a rotating shaft, and the inner wall of the handle (18) is rotatably connected with a positioning block (19).

7. A device for freezing cells by gaseous phase liquid nitrogen according to claim 6, wherein: The inner wall of the positioning block (19) is rotatably connected with a damping rotating shaft (20), and the outer side of the damping rotating shaft (20) is rotatably connected with the inner wall of the fixing frame (15).

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