Plant living body imaging and simulation illumination device

By designing a plant in vivo imaging device that includes a housing, a CCD camera, and environmental simulation components, and simulating natural light and temperature, the problem of inaccurate data in existing technologies is solved, enabling more precise biological research.

CN223784188UActive Publication Date: 2026-01-09科辰星飞(北京)科技有限公司
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Patent Information

Application Number
CN202422795017.2
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-11-16
Publication Date
2026-01-09
Estimated Expiration
2034-11-16

AI Technical Summary

Technical Problem

Existing plant in vivo imaging devices cannot simulate temperature, light, and humidity conditions close to those in the natural environment, resulting in inaccurate biological research data.

Method used

A device comprising a housing, a CCD camera, and an environmental simulation component was designed. It simulates natural light using an LDE plant growth lamp and colored lenses, and captures fluorescence signals using a CCD camera to achieve fluorescent labeling and monitoring of genes in living plants.

Benefits of technology

Creating conditions that more closely resemble the natural environment allows for more precise biological research data, enabling the detection of changes in fluorescence intensity and the identification of enhancers or repressors.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to the technical field of plant living body imaging, and discloses a plant living body imaging and simulation illumination device which comprises a box body, when the device is used, an LED plant growth lamp can be turned on firstly through an arranged environment simulation assembly, and light irradiates a plant through a through hole; light is dispersed around plants through the astigmatic lenses arranged on the through holes, so that more real natural light is simulated, meanwhile, natural light such as sunny days, cloudy days and night with moonlight can be simulated through the colored lenses, and an environment closer to the natural environment is created. According to the present invention, with the arrangement of the CCD camera, the weak fluorescence signal can be captured, the specific gene of the living plant can be subjected to fluorescence labeling, and after the EMS mutagenesis treatment, the fluorescence brightness change can be detected in the imaging device so as to find the enhancer or the suppressor, such that the biological research data can be obtained; and a better use prospect can be brought.
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Description

TECHNICAL FIELD

[0001] The utility model relates to plant living body imaging technical field, concretely is a kind of plant living body imaging and its simulated light device. BACKGROUND

[0002] The principle of plant living body imaging device is mainly based on fluorescence labeling technology and imaging technology, and the fluorescence labeling technology is the basis of plant living body imaging. Scientists label fluorescent proteins or fluorescent dyes to specific genes or proteins through genetic engineering. When these markers are excited by light of a specific wavelength, they will emit fluorescence, which can be detected in imaging equipment. Commonly used fluorescent markers include green fluorescent protein (GFP) and red fluorescent protein (RFP).

[0003] However, it still has some shortcomings, for example: when the traditional plant living body imaging device monitors specific molecules and genes in the plant body in real time, the environment where the plant is located is in a black box, and it is not possible to simulate experimental conditions closer to the natural environment, such as temperature, light and humidity simulation. Among them, the light intensity and the simulation of sunny and cloudy days in the natural environment, under the irradiation for a long time, the living plant genes will change greatly, so that the detected biological research data is not accurate.

[0004] To solve the above problems, a plant living body imaging and simulated light device is proposed in this application. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a plant living body imaging and simulated light device to solve the problems in the prior art, such as the plant living body imaging device in the prior art cannot simulate experimental conditions closer to the natural environment, such as temperature, light and humidity simulation, and the light intensity, and under the irradiation for a long time, the living plant genes will change greatly, so that the detected biological research data is not accurate.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a plant living body imaging and simulated light device, comprising a box, the front end outer surface of the box is movably connected with a box door, the front end outer surface of the box door is fixedly connected with a light-tight partition, the upper end outer surface of the box is fixedly connected with an environment simulation assembly, and the outer surface of one side of the box is fixedly connected with a light-tight and breathable plate.

[0007] Preferably, the inner cavity of the box is fixedly connected with a circuit board shell, the lower end outer surface of the circuit board shell is fixedly connected with a base and a CCD camera, and the base is located on the upper end outer surface of the CCD camera.

[0008] Preferably, the inner cavity of the box is placed with green plants, and the base and the CCD camera are located above the green plants, the upper end of the outer surface of the box is provided with a through hole, and the inner wall of the through hole is fixedly connected with a diffuser lens.

[0009] Preferably, the environment simulation assembly comprises a sleeve, a limiting snap ring, a lens outer frame, an LED lamp shell, an LDE plant growth lamp, a groove, a clamping groove and a colored lens, the sleeve is fixedly connected to the upper end of the outer surface of the box, the inner wall of the sleeve is fixedly connected with the limiting snap ring, and the inner wall of the limiting snap ring is detachably connected with the lens outer frame.

[0010] Preferably, the upper end of the outer surface of the sleeve is fixedly connected with the LED lamp shell, the inner cavity of the LED lamp shell is fixedly connected with the LDE plant growth lamp, and the outer wall of the sleeve is provided with the groove.

[0011] Preferably, the upper end of the outer surface of the lens outer frame is provided with the clamping groove, and the inner surface of the clamping groove is provided with the colored lens.

[0012] Compared with the prior art, the plant live body imaging and simulated light device has the advantages that:

[0013] The CCD camera can capture weak fluorescent signals, and the specific genes of the live plants are marked with fluorescence, so that the changes in fluorescent brightness in the imaging equipment are checked after EMS mutagenesis treatment, and the enhancer or suppressor is found.

[0014] The environment simulation assembly is provided, the LDE plant growth lamp is turned on first, the light is irradiated on the plants through the through hole, the light is dispersed around the plants through the diffuser lens fixedly connected to the through hole, so that more real natural light is simulated, the colored lens is provided, the sunny day and the cloudy day, the moonlight night and other natural light are simulated, a more natural environment is created, and the detected biological research data is closer to the data of the plant growth in the natural environment. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a structural schematic view of the plant live body imaging and simulated light device;

[0016] Figure 2 It is an internal structure schematic view of the plant live body imaging and simulated light device;

[0017] Figure 3 It is a structural schematic view of the CCD camera in the plant live body imaging and simulated light device;

[0018] Figure 4The utility model provides a kind of plant live body imaging and its simulated illumination device environmental simulation subassembly structure schematic view.

[0019] In the figure: 1, box body;2, box door;3, light-tight partition;4, environmental simulation subassembly;5, light-tight gas-permeable plate;6, circuit board shell;7, base;8, CCD camera;9, green plant;10, through hole;11, diffuser lens;12, sleeve;13, limit snap ring;14, lens outer frame;15, LED lamp shell;16, LDE plant growth lamp;17, recess;18, clamping groove;19, colored lens. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments.

[0021] Please refer to Figures 1-4 The utility model provides a kind of technical scheme: a kind of plant live body imaging and its simulated illumination device, including box body 1, the front end outer surface of box body 1 is movably connected with box door 2, the front end outer surface of box door 2 is fixedly connected with light-tight partition 3, the upper end outer surface of box body 1 is fixedly connected with environmental simulation subassembly 4, the outer surface of one side of box body 1 is fixedly connected with light-tight gas-permeable plate 5.

[0022] In the embodiment, as Figures 2-3 Indicated, the inner cavity of box body 1 is fixedly connected with circuit board shell 6, the lower end outer surface of circuit board shell 6 is fixedly connected with base 7 and CCD camera 8, and base 7 is located on the upper end outer surface of CCD camera 8, by setting CCD camera 8, can capture weak fluorescence signal, the specific gene of live body plant is marked with fluorescence, after EMS mutagenesis treatment, check the change of fluorescence brightness in imaging equipment, find enhancer or suppressor box body 1, and base 7 and CCD camera 8 are located above green plant 9, through hole 10 is set on the upper end outer surface of box body 1, the inner wall of through hole 10 is fixedly connected with diffuser lens 11, by setting diffuser lens 11, can spread the light irradiated by LDE plant growth lamp 16 around plant, so that plant is in a more natural environment illumination.

[0023] In the embodiment, as Figures 3-4As shown, the environment simulation assembly 4 comprises a sleeve 12, a limiting snap ring 13, a lens outer frame 14, an LED lamp shell 15, an LDE plant growth lamp 16, a groove 17, a clamping groove 18 and a colored lens 19, the sleeve 12 is fixedly connected to the upper end outer surface of the box body 1, the inner wall of the sleeve 12 is fixedly connected with the limiting snap ring 13, the inner wall of the limiting snap ring 13 is detachably connected with the lens outer frame 14, through the lens outer frame 14, we can conveniently clamp the colored lens 19 in the middle part of the sleeve 12, the upper end outer surface of the sleeve 12 is fixedly connected with the LED lamp shell 15, the inner cavity of the LED lamp shell 15 is fixedly connected with the LDE plant growth lamp 16, the outer wall of the sleeve 12 is provided with the groove 17, the upper end outer surface of the lens outer frame 14 is provided with the clamping groove 18, and the inner surface of the clamping groove 18 is provided with the colored lens 19, through the clamping groove 18, we can conveniently place the colored lens 19 of different colors in the sleeve 12.

[0024] Working principle:

[0025] A plant living body imaging and simulation lighting device thereof, in use, first, through the environment simulation assembly 4, the LDE plant growth lamp 16 is turned on, the light is irradiated on the green plant 9 through the through hole 10, the light is dispersed around the green plant 9 through the fixedly connected light dispersing lens 11 on the through hole 10, so that a more real natural light is simulated, the colored lens 19 is placed in the clamping groove 18, the lens outer frame 14 is clamped in the limiting snap ring 13, the colored lens 19 is placed on the line irradiated by the LDE plant growth lamp 16, the color of the light irradiated by the LDE plant growth lamp 16 is changed through the colored lens 19, the sunny day and the cloudy day and the natural light with moonlight night are simulated, a more natural environment is created, the detected biological research data is closer to the data of plant growth in the natural environment, and the CCD camera 8 can capture weak fluorescent signals, the specific genes of the living plant are fluorescently labeled, the change of fluorescent brightness is checked in the imaging equipment after EMS mutagenesis treatment, and the enhancer or the suppressor is found.

[0026] Based on the embodiments in the utility model, all other embodiments obtained by the ordinary skilled person in the art without making creative labor belong to the protection range of the utility model.

Claims

1. A plant live body imaging and its simulated light illumination device comprising a cabinet (1), characterized in that: The outer surface of the front end of the box (1) is movably connected with a box door (2), the outer surface of the front end of the box door (2) is fixedly connected with a light-tight partition (3), the outer surface of the upper end of the box (1) is fixedly connected with an environment simulation assembly (4), the outer surface of one side of the box (1) is fixedly connected with a light-tight and breathable plate (5), the environment simulation assembly (4) comprises a sleeve (12), a limiting snap ring (13), a lens outer frame (14), an LED lamp shell (15), an LDE plant growth lamp (16), a groove (17), a clamping groove (18) and a colored lens (19), the sleeve (12) is fixedly connected to the outer surface of the upper end of the box (1), the inner wall of the sleeve (12) is fixedly connected with the limiting snap ring (13), and the inner wall of the limiting snap ring (13) is detachably connected with the lens outer frame (14).

2. The apparatus of claim 1, wherein: The inner cavity of the box (1) is fixedly connected with a circuit board shell (6), the lower end of the outer surface of the circuit board shell (6) is fixedly connected with a base (7) and a CCD camera (8), and the base (7) is located on the outer surface of the upper end of the CCD camera (8).

3. An apparatus according to claim 2, wherein: The inner cavity of the box (1) is fixedly connected with a circuit board shell (6), the lower end of the outer surface of the circuit board shell (6) is fixedly connected with a base (7) and a CCD camera (8), and the base (7) is located on the outer surface of the upper end of the CCD camera (8).

4. The apparatus of claim 1, wherein: The inner cavity of the box (1) is fixedly connected with a circuit board shell (6), the lower end of the outer surface of the circuit board shell (6) is fixedly connected with a base (7) and a CCD camera (8), and the base (7) is located on the outer surface of the upper end of the CCD camera (8).

5. The apparatus of claim 1, wherein: The outer surface of the upper end of the sleeve (12) is fixedly connected with the LED lamp shell (15), the inner cavity of the LED lamp shell (15) is fixedly connected with the LDE plant growth lamp (16), and the outer wall of the sleeve (12) is provided with the groove (17). The outer surface of the upper end of the sleeve (12) is fixedly connected with the LED lamp shell (15), the inner cavity of the LED lamp shell (15) is fixedly connected with the LDE plant growth lamp (16), and the outer wall of the sleeve (12) is provided with the groove (17).