Uniform mixing mechanism of nucleic acid extractor

Abstract

The utility model belongs to the technical field of nucleic acid extraction instruments, and particularly relates to a mixing mechanism of a nucleic acid extraction instrument, which comprises a base and a magnetic bar arranged on one side of the base and extending along the length direction of the base, and the base is provided with a mixing mechanism used for being connected with the magnetic bar and driving the magnetic bar to rotate. And the uniform mixing mechanism is driven by a driving assembly arranged on the base, so that the magnetic bar circumferentially rotates along the axis of the magnetic bar. Through the arrangement of the uniform mixing mechanism and the driving assembly, when the driving assembly moves, the uniform mixing mechanism can drive the magnetic bar to rotate in the circumferential direction, so that when the magnetic bead-nucleic acid compound is inserted into a container filled with a magnetic bead-nucleic acid compound, the magnetic bar rotates to drive surrounding liquid to move along with the magnetic bead-nucleic acid compound, and an effect similar to a stirring effect is generated; because the magnetic beads suspend in the solution, the flowing of the liquid enables the magnetic beads to continuously roll and collide in the solution, so that the magnetic beads are promoted to be in full contact with nucleic acid in a sample, and the extraction efficiency and purity of the nucleic acid are effectively improved.

Description

Technical Field

[0001] This utility model belongs to the technical field of nucleic acid extraction instruments, specifically relating to the mixing mechanism of a nucleic acid extraction instrument. Background Technology

[0002] A nucleic acid extractor is an instrument that can automatically extract nucleic acids from biological samples (such as blood, tissues, cells, swabs, etc.). Its basic function is to separate nucleic acids from complex biological sample matrices through a series of physical and chemical methods, providing pure nucleic acid samples for subsequent nucleic acid detection, gene analysis, and other experiments.

[0003] Nucleic acid extractors often employ the magnetic bead method. Magnetic beads are added to samples containing nucleic acids. The surface of these beads is typically modified with groups that specifically adsorb nucleic acids. For example, during DNA extraction, the magnetic beads have groups that can bind to the DNA phosphate backbone. When the sample and magnetic beads are thoroughly mixed, the nucleic acids are adsorbed onto the bead surface. The control system within the nucleic acid extractor then moves a magnetic rod to the reaction vessel containing the magnetic beads and the nucleic acid sample. The magnetic rod then activates a magnetic field, adsorbing the magnetic beads onto its surface.

[0004] Currently, existing nucleic acid extractors can only move the magnetic rod up and down to insert it into the reaction vessel. However, this vertical insertion of the magnetic rod may cause the magnetic beads to aggregate unevenly at the bottom of the reaction vessel. This is because the liquid flow generated during the insertion process is mainly in the insertion direction, which causes the magnetic beads to be pushed to one side or the center of the bottom of the vessel, rather than being evenly distributed at the bottom. Therefore, during subsequent magnetic separation operations, this uneven distribution may affect the complete adsorption of the magnetic bead-nucleic acid complex, reducing the efficiency and purity of nucleic acid extraction.

[0005] In view of this, the present invention provides a mixing mechanism for a nucleic acid extractor to solve the above problems. Utility Model Content

[0006] To achieve the above objectives, the present invention provides the following technical solution: a mixing mechanism for a nucleic acid extractor, comprising a base and a magnetic rod disposed on one side of the base and extending along its length, characterized in that: a mixing mechanism for connecting the magnetic rod and driving the magnetic rod to rotate is provided on the base, and the mixing mechanism is driven by a driving component disposed on the base to make the magnetic rod rotate circumferentially along its axis.

[0007] As a preferred embodiment of the mixing mechanism of the nucleic acid extractor of this utility model, the mixing mechanism includes a first substrate and a second substrate arranged opposite to each other, a driving wheel with the same axis arranged rotatably connected between the first substrate and the second substrate, a driven wheel for axially fixing a magnetic rod, and a mixing synchronous belt connected between the driving wheel and the driven wheel.

[0008] The drive wheel has a connecting shaft inserted movably along its axial direction inside for driving its circumferential rotation, and a drive auxiliary wheel is fixedly connected to the top of the connecting shaft.

[0009] As a preferred embodiment of the mixing mechanism of the nucleic acid extractor of this utility model, the driving component includes a first driving member and a driving main wheel connected to the movable end of the first driving member, wherein the driving main wheel is connected to the driving secondary wheel through a driving timing belt.

[0010] As a preferred embodiment of the mixing mechanism of the nucleic acid extractor of this utility model, the base is further provided with a lifting component for driving the first substrate and the second substrate to move synchronously along the axial direction of the connecting shaft.

[0011] As a preferred embodiment of the mixing mechanism of the nucleic acid extractor of this utility model, the lifting assembly includes a second driving member whose movable end makes circular motion, a lifting main wheel connected to the movable end of the second driving member, and a lifting secondary wheel rotatably connected to the base. The lifting main wheel and the lifting secondary wheel are connected by a lifting synchronous belt.

[0012] It also includes: a connector, one end of which is connected to the lifting synchronous belt, and the other end of which is synchronously connected to the first substrate and the second substrate.

[0013] As a preferred embodiment of the mixing mechanism of the nucleic acid extractor of this utility model, a guide component for stabilizing the movement of the first substrate and the second substrate is further provided between the mixing mechanism and the base.

[0014] As a preferred embodiment of the mixing mechanism of the nucleic acid extractor of this utility model, the guiding component includes a bearing seat disposed opposite to the connecting shaft for rotatable connection, and a guide rail disposed between the bearing seats, wherein a slider for synchronous connection with the first substrate and the second substrate is slidably connected on the guide rail.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] This invention, through the design of a mixing mechanism and a driving component, enables the mixing mechanism to drive the magnetic rod to rotate circumferentially when the driving component moves. When the magnetic rod is inserted into a container containing the magnetic bead-nucleic acid complex, the rotation of the magnetic rod causes the surrounding liquid to move, producing a stirring-like effect. Since the magnetic beads are suspended in the solution, the flow of the liquid causes the magnetic beads to continuously roll and collide in the solution, thereby promoting full contact between the magnetic beads and the nucleic acid in the sample, effectively improving the efficiency and purity of nucleic acid extraction. Attached Figure Description

[0017] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0018] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0019] Figure 2 This is a structural schematic diagram of the present invention from another perspective;

[0020] Figure 3 This is a schematic diagram of the base structure of this utility model;

[0021] Figure 4 This is a schematic diagram of the mixing mechanism of this utility model;

[0022] Figure 5 This is a schematic diagram of the drive component structure of this utility model;

[0023] Figure 6 This is a schematic diagram of the lifting component structure of this utility model;

[0024] Figure 7 This is a schematic diagram of the guide component structure of this utility model.

[0025] In the figure: 1. Base; 101. First sidewall; 102. Second sidewall; 103. Third sidewall; 2. Magnetic rod; 3. Mixing mechanism; 301. First substrate; 302. Second substrate; 303. Substrate fixing end block; 304. Driving wheel; 305. Driven wheel; 306. Mixing synchronous belt; 307. Connecting shaft; 308. Drive auxiliary wheel; 4. Drive assembly; 401. First driving component; 402. Drive main wheel; 403. Drive synchronous belt; 5. Lifting assembly; 501. Second driving component; 502. Lifting main wheel; 503. Lifting auxiliary wheel; 504. Lifting synchronous belt; 505. Connector; 6. Guide assembly; 601. Shaft seat; 602. Guide rail; 603. Slider. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] This utility model relates to the mixing mechanism of a nucleic acid extractor, such as... Figures 1-2 As shown, it includes a base 1 and a magnetic rod 2 disposed on one side of the base 1 and extending along its length. A mixing mechanism 3 is provided on the base 1 for connecting the magnetic rod 2 and driving the magnetic rod 2 to rotate. The mixing mechanism 3 is driven by a driving component 4 disposed on the base 1 to make the magnetic rod 2 rotate circumferentially along its axis.

[0028] Specifically, such as Figures 3-5 As shown, the base 1 has a first sidewall 101 and a second sidewall 102 arranged opposite to each other, and a third sidewall 103 connecting the first sidewall 101 and the second sidewall 102; the mixing mechanism 3 is disposed on the surface of the third sidewall 103, which includes a first substrate 301 and a second substrate 302 arranged opposite to each other, and a substrate fixing end block 303 connected to the end faces of the first substrate 301 and the second substrate 302 for fixing the first substrate 301 and the second substrate 302. At least two sets of through holes corresponding to each other are opened on the surface of the first substrate 301 and the second substrate 302. A set of driving wheels 304 arranged in the same direction and at least one set of driven wheels 305 for axially fixing the sleeved magnetic rod 2 are respectively connected between the corresponding through holes of the first substrate 301 and the second substrate 302. The driving wheels 304 and the driven wheels 305 are connected by a mixing synchronous belt 306 so that when the driving wheel 304 rotates, the driven wheel 305 will rotate synchronously with the driving wheel 304. A connecting shaft 307 for driving its circumferential rotation is movably inserted inside the drive wheel 304 along its axial direction. The two ends of the connecting shaft 307 and the magnetic rod 2 pass through corresponding through holes opened on the surfaces of the first substrate 301 and the second substrate 302, respectively, forming the rotation shafts of the drive wheel 304 and the driven wheel 305, so that the drive wheel 304 and the driven wheel 305 are rotatably connected between the first substrate 301 and the second substrate 302. The radial cross section of the connecting shaft 307 is a polygonal structure.

[0029] In this embodiment, the radial cross-section of the connecting shaft 307 is hexagonal, and the interior of the driving wheel 304 has a through hole that matches the radial cross-sectional size of the connecting shaft 307. A drive auxiliary wheel 308 for connection to the drive assembly 4 is axially fixedly connected to the top end of the connecting shaft 307. The drive assembly 4 is located on the back side of the third sidewall 103, as shown below. Figure 7 As shown, the drive assembly 4 includes a first drive member 401 whose movable end makes circular motion, and a drive main wheel 402 axially connected to the movable end of the first drive member 401. The drive main wheel 402 is connected to the drive auxiliary wheel 308 through a drive timing belt 403. In this embodiment, the first drive member 401 is a motor.

[0030] In use, the movable end of the first driving component 401 rotates circumferentially to drive the main driving wheel 402 to rotate. Under the action of the driving synchronous belt 403, the secondary driving wheel 308 and the connecting shaft 307 rotate circumferentially. Since the connecting shaft 307 passes through the driving wheel 304, when the connecting shaft 307 rotates, the driving wheel 304 will rotate synchronously, ultimately realizing the rotation of the magnetic rod 2. Therefore, during nucleic acid extraction, when the magnetic rod 2 is inserted into the container containing the magnetic bead-nucleic acid complex, the rotation of the magnetic rod 2 will drive the surrounding liquid to move accordingly, producing a stirring-like effect. Since the magnetic beads are suspended in the solution, the flow of the liquid will cause the magnetic beads to continuously roll and collide in the solution, thereby promoting full contact between the magnetic beads and the nucleic acid in the sample, effectively improving the efficiency and purity of nucleic acid extraction.

[0031] Furthermore, in order to facilitate the insertion and extension of the magnetic rod 2 into and out of the container containing the magnetic bead-nucleic acid complex, a lifting assembly 5 is also provided at the second side wall 102 for driving the first substrate 301 and the second substrate 302 to move synchronously along the axial direction of the connecting shaft 307.

[0032] like Figure 6 As shown, the lifting assembly 5 includes a second driving member 501, a lifting main wheel 502 connected to the movable end of the second driving member 501, and a lifting auxiliary wheel 503 rotatably connected to the second side wall 102. The lifting main wheel 502 and the lifting auxiliary wheel 503 are connected by a lifting synchronous belt 504. It also includes a connecting member 505, one end of which is fixedly connected to the lifting synchronous belt 504, and the other end of which is synchronously connected to the first base plate 301 and the second base plate 302.

[0033] In this embodiment, the line connecting the midpoint of the lifting main wheel 502 and the center of the lifting auxiliary wheel 503 is parallel to the length extension direction of the base 1. The second driving component 501 is a motor, whose movable end drives the lifting main wheel 502 to rotate circumferentially. Under the action of the lifting synchronous belt 504, the lifting main wheel 502 drives the lifting auxiliary wheel 503 to rotate. Since the lifting synchronous belt 504 will inevitably move when the lifting main wheel 502 rotates, when the connecting member 505 is connected to a certain position on the surface of the lifting synchronous belt 504, the connecting member 505 will move with the lifting synchronous belt 504, so as to make the first substrate 301 and the second substrate 302 rise and fall synchronously, so as to realize the magnetic rod 2 to move up and down along its axial direction.

[0034] Furthermore, to improve the stability of the first substrate 301 and the second substrate 302 during movement, a guide component 6 is also provided between the mixing mechanism 3 and the base 1, such as... Figure 7As shown, it includes a bearing seat 601 disposed opposite to the surface of the third sidewall 103, and a guide rail 602 disposed on the surface of the third sidewall 103 and located between the two bearing seats 601. A slider 603 for synchronous connection with the first substrate 301 and the second substrate 302 is slidably connected to the guide rail 602. The two ends of the connecting shaft 307 are rotatably connected to the two bearing seats 601 respectively. In use, when the lifting assembly 5 drives the first substrate 301 and the second substrate 302 to move up and down synchronously, the slider 603 can slide along the guide rail 602.

[0035] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A mixing mechanism for a nucleic acid extractor, comprising a base (1) and a magnetic rod (2) disposed on one side of the base (1) and extending along its length, characterized in that: The base (1) is provided with a mixing mechanism (3) for connecting the magnetic rod (2) and driving the magnetic rod (2) to rotate. The mixing mechanism (3) is driven by the driving component (4) provided on the base (1) to make the magnetic rod (2) rotate circumferentially along its axis.

2. The mixing mechanism of the nucleic acid extractor according to claim 1, characterized in that: The mixing mechanism (3) includes a first substrate (301) and a second substrate (302) arranged opposite to each other, a driving wheel (304) and a driven wheel (305) for axially fixing the magnetic rod (2) are rotatably connected between the first substrate (301) and the second substrate (302), and a mixing synchronous belt (306) connected between the driving wheel (304) and the driven wheel (305); The drive wheel (304) has a connecting shaft (307) inserted movably along its axial direction inside for driving its circumferential rotation, and a drive auxiliary wheel (308) is fixedly connected to the top end of the connecting shaft (307).

3. The mixing mechanism of the nucleic acid extractor according to claim 2, characterized in that: The drive assembly (4) includes a first drive member (401) and a drive main wheel (402) connected to the movable end of the first drive member (401). The drive main wheel (402) is connected to the drive auxiliary wheel (308) via a drive timing belt (403).

4. The mixing mechanism of the nucleic acid extractor according to claim 2, characterized in that: The base (1) is also provided with a lifting assembly (5) for driving the first substrate (301) and the second substrate (302) to move synchronously along the axial direction of the connecting shaft (307).

5. The mixing mechanism of the nucleic acid extractor according to claim 4, characterized in that: The lifting assembly (5) includes a second drive member (501) whose movable end makes circular motion, a lifting main wheel (502) connected to the movable end of the second drive member (501), and a lifting auxiliary wheel (503) rotatably connected to the base (1). The lifting main wheel (502) and the lifting auxiliary wheel (503) are connected by a lifting synchronous belt (504). It also includes a connector (505), one end of which is connected to the lifting synchronous belt (504), and the other end of which is synchronously connected to the first substrate (301) and the second substrate (302).

6. The mixing mechanism of the nucleic acid extractor according to claim 4, characterized in that: A guide component (6) for stabilizing the movement of the first substrate (301) and the second substrate (302) is also provided between the mixing mechanism (3) and the base (1).

7. The mixing mechanism of the nucleic acid extractor according to claim 6, characterized in that: The guide assembly (6) includes a bearing seat (601) disposed opposite to the connecting shaft (307) for rotatable connection, and a guide rail (602) disposed between the bearing seats (601), wherein a slider (603) for synchronous connection with the first substrate (301) and the second substrate (302) is slidably connected on the guide rail (602).

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