Purification instrument

Abstract

The utility model discloses a purification instrument, which comprises a rotary table, at least one group of pore plates, at least one group of pore plates, at least one group of pore plates and at least one group of pore plates, the magnetic bar sleeve is configured to move in the vertical direction so as to enter or exit from the pore plate at a preset position; the magnetic bar is arranged above the magnetic bar sleeve and can move into or out of the magnetic bar sleeve along the vertical direction; the magnetic bead purification instrument can solve the problems that a traditional magnetic bead purification instrument needs to use a large number of magnetic beads at a time, whether the magnetic beads are lost or not is difficult to find, then the purification effect of a sample solution is difficult to guarantee, and a large number of magnetic beads are used and difficult to take and place.

Description

TECHNICAL FIELD

[0001] The utility model relates to purification equipment technical field, concretely relates to a kind of purifier. BACKGROUND

[0002] Magnetic bead purifier is a kind of equipment using magnetic beads to separate and purify biomolecules (such as nucleic acid, protein and cell), and is widely used in biological medicine, genetic engineering, environmental monitoring and other fields.

[0003] When the traditional magnetic bead purifier purifies sample solutions in several well plates at the same time, magnetic beads need to be placed in the hole position of each well plate, and the magnetic beads are taken out after purification is completed. This method uses a large number of magnetic beads, which makes it difficult to determine whether the magnetic beads are missing and thus affects the purification effect of the sample solution. In addition, using a large number of magnetic beads makes it difficult to take them out. UTILITY MODEL CONTENT

[0004] To overcome the above-mentioned shortcomings, the purpose of the utility model is to provide a purifier.

[0005] To achieve the above purpose, the utility model adopts the technical scheme comprising: a turntable configured to rotate along its axial direction, at least one set of well plates is arranged on the turntable; a magnetic rod sleeve configured to move vertically into or out of the well plate at a predetermined position; a magnetic rod configured above the magnetic rod sleeve and movable vertically into or out of the magnetic rod sleeve.

[0006] The present application places multiple sets of well plates on the turntable and uses only one set of magnetic beads with the magnetic rod, which realizes the transfer in multiple well plates, improves the purification efficiency of sample solutions, reduces the use of magnetic beads, and reduces the subsequent steps of taking out the magnetic beads in multiple well plates. It has the characteristics of simple structure, convenient operation and practicality.

[0007] In the preferred technical scheme of the above purifier, the magnetic rod sleeve and the magnetic rod are arranged on the frame, the frame is located above the turntable and does not rotate synchronously with the turntable, the frame is provided with a first driving device for driving the magnetic rod sleeve to move vertically and a second driving device for driving the magnetic rod to move vertically.

[0008] In the preferred technical scheme of the above purifier, the turntable is driven to rotate by a third driving device.

[0009] In the preferred technical scheme of the above purifier, the magnetic rod sleeve is arranged on the frame through a bearing seat, and the magnetic rod sleeve is detachably connected to the bearing seat through a quick-change structure.

[0010] In the preferred technical scheme of the above-mentioned purifier, the plurality of magnetic rods are detachably connected to the second driving device through the base plate.

[0011] In the preferred technical scheme of the above-mentioned purifier, the quick-change structure at least comprises rotating rods arranged on both sides of the bearing seat along the length direction of the bearing seat, clamping jaws arranged on the rotating rods and synchronously rotating with the rotating rods, and a fourth driving device arranged on the bearing seat and driving the two rotating rods to synchronously rotate in opposite directions.

[0012] In the preferred technical scheme of the above-mentioned purifier, the purifier further comprises a collecting piece capable of entering between the well plate and the magnetic rod sleeve and used for collecting liquid dripping from the surface of the magnetic rod sleeve.

[0013] In the preferred technical scheme of the above-mentioned purifier, the collecting piece is driven by a fifth driving device to enter or exit between the magnetic rod sleeve and the well plate.

[0014] In the preferred technical scheme of the above-mentioned purifier, the collecting piece is a collecting plate with a groove.

[0015] In the preferred technical scheme of the above-mentioned purifier, the surface of the collecting piece is sprayed with a polytetrafluoroethylene coating.

[0016] The purifier has the advantages that the plurality of well plates are placed on the rotating disc, only one set of magnetic beads is used in cooperation with the magnetic rod, the transfer in the plurality of well plates is realized, the purification efficiency of the sample solution is improved, the use of the magnetic beads is reduced, the step of taking out the magnetic beads in the plurality of well plates is reduced, the structure is simple, the operation is convenient, and the purifier has practicality. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a front view of the shell;

[0018] Figure 2 It is a front view of the inside of the shell Figure 1 ;

[0019] Figure 3 It is a front view of the inside of the shell Figure 2 ;

[0020] Figure 4 It is a front view of the first driving device and the magnetic rod sleeve, and the second driving device and the magnetic rod on the frame Figure 1 ;

[0021] Figure 2 It is a front view of the first driving device and the magnetic rod sleeve, and the second driving device and the magnetic rod on the frame Figure 6 ;

[0022] Figure 1 It is a schematic of the quick-change structureFigure 7 ;

[0023] Figure 2 For the schematic of quick-change structure Figures 1 to 7 ;

[0024] In the figure: turntable 1, hole plate 2, magnetic rod sleeve 3, magnetic force rod 4, shell 5, frame body 6, first driving device 7, second driving device 8, third driving device 9, bearing seat 10, rotating rod 11, clamping jaw 12, fourth driving device 13, base plate 14, collection piece 15, fifth driving device 16. DETAILED DESCRIPTION

[0025] The preferred embodiments of the present application will be described below with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are only used to explain the technical principles of the present application, and are not intended to limit the protection scope of the present application.

[0026] It should be noted that in the description of the present application, the terms "upper", "lower", "left", "right", "front", "back" and other terms indicating direction or positional relationship are based on the direction or positional relationship shown in the drawings, which is only for the convenience of description, and is not intended to indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second" are only for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0027] In addition, it should also be noted that in the description of the present application, unless otherwise explicitly specified and limited, the terms "provided", "connected", "connected" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrally connected. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0028] As Figures 1 to 3 shown, the purifier of the present application comprises: a turntable 1 configured to rotate along the axial direction thereof, at least one set of hole plates 2 being arranged on the turntable 1; a magnetic rod sleeve 3 configured to move along the vertical direction to enter or exit the hole plate 2 at a predetermined position; a magnetic force rod 4 arranged above the magnetic rod sleeve 3 and movable along the vertical direction to enter or exit the magnetic rod sleeve 3.

[0029] Referring to Figure 1 , the turntable 1 is rotatably arranged on the shell 5, and a plurality of sets of hole plates 2 are detachably mounted on the turntable 1. When the hole plates 2 are provided with a plurality of hole plates 2, the plurality of hole plates 2 are uniformly arranged on the surface of the turntable 1 with the central axis of the turntable 1 as the center, and at least one magnetic bead is movably arranged in each hole position of one of the plurality of hole plates 2.

[0030] Referring toFigure 2 、 Figure 1 The number of magnetic rod sleeves 3 corresponds to the number of hole positions of the hole plate 2, and the magnetic rod sleeve 3 can be inserted into the hole position of the hole plate 2. The magnetic rod sleeve 3 is made of plastic material and can move downward in the vertical direction to enter the hole position of the hole plate 2, or move upward in the vertical direction to exit the hole position of the hole plate 2. By continuously controlling the upward or downward movement of the magnetic rod sleeve 3, the magnetic rod sleeve 3 can fully agitate the sample solution in the hole position of the hole plate 2, and the magnetic beads can move in the hole position of the hole plate 2, thereby further ensuring the purification effect of the sample solution.

[0031] Referring to Figure 2 、 Figures 2 to 5 The magnetic rod 4 is arranged above the magnetic rod sleeve 3. The number of magnetic rods 4 corresponds to the number of magnetic rod sleeves 3. The magnetic rod 4 is arranged to move downward in the vertical direction to enter the magnetic rod sleeve 3, or to move upward in the vertical direction to exit the magnetic rod sleeve 3. The magnetic rod 4 has a certain magnetism and can form a magnetic attraction to the magnetic beads.

[0032] When purifying the sample solution in the hole position of the hole plate 2, first fill the hole positions of several groups of hole plates 2 with sample solution, and add at least one magnetic bead to each hole position of one group of hole plates 2, then place the hole plates 2 in sequence on the predetermined position of the turntable 1. Thereafter, control the turntable 1 to rotate so that the hole plate 2 with the magnetic beads moves to the position directly below the magnetic rod sleeve 3. Control the magnetic rod sleeve 3 to repeatedly enter and exit the hole position of the hole plate 2 to form oscillation of the sample solution, thereby ensuring the purification effect of the sample solution. After purification is completed, control the magnetic rod sleeve 3 to move vertically downward into the hole position of the hole plate 2, and control the magnetic rod 4 to move downward into the magnetic rod sleeve 3. The magnetic rod 4 can adsorb the magnetic beads in the hole position of the hole plate 2. Thereafter, control the magnetic rod sleeve 3 and the magnetic rod 4 to ascend synchronously to avoid the magnetic beads from falling. When the magnetic beads exit the hole position of the hole plate 2, rotate the turntable 1 to rotate the next group of hole plates 2 to the position directly below the magnetic rod sleeve 3. Control the magnetic rod sleeve 3 and the magnetic rod 4 to move downward synchronously to make the magnetic beads enter the hole position of the group of hole plates 2. Control the magnetic rod 4 to ascend relative to the magnetic rod sleeve 3, so that the magnetic rod 4 no longer attracts the magnetic beads, and the magnetic beads fall into the hole position of the group of hole plates 2. Thereafter, repeat the above operation to purify the sample solution in the group of hole plates 2. The present application places multiple groups of hole plates 2 on the turntable 1, and only uses one group of magnetic beads in cooperation with the magnetic rod 4 to achieve transfer in multiple hole plates 2. This improves the purification efficiency of the sample solution, reduces the use of magnetic beads, and reduces the subsequent steps of taking out the magnetic beads in multiple groups of hole plates 2. The present application has the characteristics of simple structure, convenient operation and practicality.

[0033] In one or more embodiments, the magnetic rod sleeve 3 and the magnetic force rod 4 are arranged on the frame body 6, the frame body 6 is located above the rotating disc 1 and does not rotate synchronously with the rotating disc 1, and the frame body 6 is arranged with the first driving device 7 for driving the magnetic rod sleeve 3 to move in the vertical direction and the second driving device 8 for driving the magnetic force rod 4 to move in the vertical direction.

[0034] Referring to Figure 3 , the frame body 6 is installed on the shell 5 through the rotating disc 1, the position of the frame body 6 relative to the shell 5 does not change, and the frame body 6 does not rotate synchronously with the rotating disc 1; the movement of the magnetic rod sleeve 3 and the magnetic force rod 4 on the frame body 6 is relatively independent, and the magnetic force rod 4 and the magnetic rod sleeve 3 can independently move upward or downward in the vertical direction on the frame body 6, the magnetic rod sleeve 3 is driven by the first driving device 7, and the magnetic force rod 4 is driven by the second driving device 8; the first driving device 7 can be a linear module, a lead screw module, a driving cylinder or other possible driving structures, the second driving device 8 can be a linear module, a lead screw module, a driving cylinder or other possible driving sources, and the selection of the first driving device 7 and the second driving device 8 is not limited in particular, which can be selected according to actual production needs.

[0035] In one or more embodiments, the rotating disc 1 is driven to rotate by the third driving device 9. Referring to Figures 4 to 7 , the third driving device 9 can be a rotary cylinder, a gear drive module or other possible driving sources, and the type of the third driving device 9 is not limited in particular, which only needs to realize the rotation of the rotating disc 1 along the central axis.

[0036] In one or more embodiments, the magnetic rod sleeve 3 is arranged on the frame body 6 through the bearing seat 10, and the magnetic rod sleeve 3 is detachably connected with the bearing seat 10 through the quick-change structure; the quick-change structure at least includes the rotating rod 11 arranged on both sides of the bearing seat 10 in the length direction of the bearing seat 10, the clamping jaw 12 arranged on the rotating rod 11 and synchronously rotating with the rotating rod 11, and the fourth driving device 13 arranged on the bearing seat 10 and driving the two rotating rods 11 to synchronously and reversely rotate; and the plurality of magnetic force rods 4 are detachably connected with the second driving device 8 through the base plate 14.

[0037] Referring to Figure 4 , the fourth driving device 13 can be a servo motor, a first connecting rod and a second connecting rod mounted on the rotating shaft end of the servo motor and synchronously rotating, a first intermediate block rotationally connected with the first connecting rod, and a second intermediate block rotationally connected with the second connecting rod; wherein the first intermediate block synchronously rotates with one of the rotating rods 11, and the second intermediate block synchronously rotates with the other rotating rod 11.

[0038] When the clamping jaws 12 rotate along the central axis of the rotating rods 11 to clamp or release the hole plate 2, the servo motor is controlled to rotate, and the servo motor drives the first connecting rod and the second connecting rod to rotate synchronously. The first connecting rod can drive one of the rotating rods 11 to rotate through the first middle block, and the second connecting rod can drive the other rotating rod to rotate synchronously through the second middle block, so as to realize that the clamping jaws 12 located on both sides of the hole plate 2 rotate towards the hole plate 2 to clamp the hole plate 2, or the clamping jaws 12 located on both sides of the hole plate 2 move away from the hole plate 2 to release the clamping of the hole plate 2. Through the above arrangement, the structure is simple and the operation is convenient.

[0039] In other possible embodiments, the quick-change structure can also be a first air cylinder and a second air cylinder arranged on both sides of the bearing seat 10. When the hole plate 2 needs to be clamped, the extended shaft ends of the first air cylinder and the second air cylinder are controlled to extend synchronously to clamp the two side portions of the hole plate 2. When the hole plate 2 needs to be released, the extended shaft ends of the first air cylinder and the second air cylinder are controlled to retract synchronously to release the clamping of the two side portions of the hole plate 2. In this way, the hole plate 2 has the characteristics of high replacement efficiency and good clamping and fixing effect, and has practicality.

[0040] Referring to Figure 5 , Figure 2 The plurality of magnetic rods 4 are installed on the base plate 14, and the base plate 14 and the second driving device 8 can be detachably connected through bolt connection. Alternatively, the base plate 14 can be detachably connected with the second driving device 8 through clamping or buckling. The specific connection mode is not limited. The magnetic rods 4 are detachably connected to the second driving device 8 through the base plate 14, so that the magnetic rods 4 and the magnetic rod sleeve 3 can be replaced and used synchronously, and the adsorption of the magnetic rods 4 to the magnetic beads is ensured.

[0041] In one or more embodiments, the purification instrument further comprises a collection piece 15 which can enter between the hole plate 2 and the magnetic rod sleeve 3 to collect the liquid dripping from the surface of the magnetic rod sleeve 3. The collection piece 15 is driven by a fifth driving device 16 to enter or exit between the hole plate 2 and the magnetic rod sleeve 3. The collection piece 15 is a collection plate with a groove.

[0042] Referring to Figure 3 , Figure 3 The fifth driving device 16 can be a driving air cylinder, a linear module or other possible driving structure, and the specific structure is not limited. When the fifth driving device 16 drives the collection piece 15 to move to enter or exit between the hole plate 2 and the magnetic rod sleeve 3, the collection piece 15 will not interfere with the rotating disc 1.

[0043] Referring to ​When the collecting piece 15 is a collecting plate with grooves, the collecting piece 15 is connected to the driving shaft end of the fifth driving device 16 through a rod. After the sample solution in a group of the hole plates 2 is purified, the magnetic rod 4 and the magnetic rod sleeve 3 are withdrawn from the hole plate 2, and the magnetic rod sleeve 3 and the surface of the magnetic beads are attached with the sample solution. To avoid the sample solution from dropping on the rotating disc 1 or the hole plate 2 and causing pollution, the fifth driving device 16 is used to drive the collecting plate with grooves to enter between the hole plate 2 and the magnetic rod sleeve 3, so that the sample solution attached to the surface of the magnetic beads or the magnetic rod sleeve 3 can drop in the grooves of the collecting plate. When the rotating disc 1 rotates to move the next group of the hole plates 2 to the position directly below the magnetic rod sleeve 3, the fifth driving device 16 is used to control the collecting plate to exit between the hole plate 2 and the magnetic rod sleeve 3, and then the above-mentioned purification steps can be performed again. In this way, the sample solution dropped on the magnetic rod sleeve 3 and the magnetic beads can be effectively collected, the use effect of the application is improved, and the application has practicality.

[0044] In one possible implementation, the collecting piece 15 is detachably installed on the driving shaft end of the fifth driving device 16. In this way, the waste liquid collected by the collecting piece 15 can be cleaned, and the possibility of the sample solution in the hole plate 2 being polluted is reduced.

[0045] In one or more implementations, the surface of the collecting piece 15 is sprayed with a polytetrafluoroethylene coating. The polytetrafluoroethylene coating has a self-lubricating effect, which can ensure that the sample solution dropped in the collecting piece 15 is easy to clean.

[0046] The above implementations are only for illustrating the technical concept and characteristics of the application, the purpose is to enable those skilled in the art to understand the content of the application and implement it, and cannot limit the protection scope of the application. Any equivalent changes or modifications made according to the spirit and essence of the application should be covered within the protection scope of the application.

Claims

1. A purification instrument, characterized in that, The application relates to a purification instrument, which comprises the following parts: a rotating disc configured to rotate along an axial direction, wherein at least one group of hole plates is arranged on the rotating disc; a magnetic rod sleeve configured to move along a vertical direction to enter or exit the hole plates at a predetermined position; a magnetic force rod arranged above the magnetic rod sleeve and movable along a vertical direction to enter or exit the magnetic rod sleeve; the magnetic rod sleeve and the magnetic force rod are arranged on a frame body, the frame body is located above the rotating disc and does not rotate synchronously with the rotating disc, the frame body is provided with a first driving device for driving the magnetic rod sleeve to move along a vertical direction and a second driving device for driving the magnetic force rod to move along a vertical direction; the rotating disc is driven to rotate by a third driving device; the magnetic rod sleeve is arranged on the frame body through a bearing seat, and the magnetic rod sleeve is detachably connected with the bearing seat through a quick-change structure.

2. The purification instrument of claim 1, wherein: a plurality of magnetic force rods are detachably connected with the second driving device through a base plate.

3. The purification instrument of claim 1, wherein: the quick-change structure at least comprises rotating rods arranged on both sides of the bearing seat along a length direction of the bearing seat, clamping jaws arranged on the rotating rods and rotating synchronously with the rotating rods, and a fourth driving device arranged on the bearing seat and driving two rotating rods to rotate reversely synchronously.

4. The purification instrument according to any one of claims 1-3, characterized in that: the purification instrument further comprises a collecting piece which can enter between the hole plates and the magnetic rod sleeve and is used for collecting liquid dripping on the surface of the magnetic rod sleeve.

5. The purification instrument of claim 4, wherein: the collecting piece is driven by a fifth driving device to enter or exit between the magnetic rod sleeve and the hole plates.

6. The purification instrument of claim 4, wherein: the collecting piece is a collecting plate with a groove.

7. The purification instrument of claim 4, wherein: the surface of the collecting piece is sprayed with a polytetrafluoroethylene coating.

Images