Enzymolysis system for rapid heating and homogenizing of PHA
By combining a steam ejector and a homogenizer, the problems of slow heating of PHA fermentation broth and low enzymatic hydrolysis rate are solved, achieving rapid cell wall breaking and efficient enzymatic hydrolysis, which is suitable for large-scale industrial production.
Patent Information
- Application Number
- CN202520141125.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-21
- Publication Date
- 2026-01-23
- Estimated Expiration
- 2035-01-21
AI Technical Summary
Existing PHA fermentation broth has a slow heating rate, high steam energy consumption, requires a lot of equipment, high investment, and is highly dependent on stirring devices, resulting in a slow enzymatic hydrolysis rate.
A steam ejector is used to rapidly heat the fermentation broth, and a homogenizer is used for homogenization, reducing reliance on stirring devices and improving enzymatic hydrolysis efficiency.
It achieves rapid heating and homogenization of cell walls, reduces steam consumption and production costs, increases enzymatic hydrolysis rate, and is suitable for large-scale industrial production.
Smart Images
Figure CN223823597U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of enzymatic hydrolysis equipment technology, specifically relating to an enzymatic hydrolysis system for rapid heating and homogenization of PHA. Background Technology
[0002] PHA (polyhydroxyalkanoate) is a general term for high-molecular-weight polyesters produced by microbial fermentation using various carbon sources. Due to its excellent biocompatibility, biodegradability, and thermal processing properties, it can serve as an environmentally friendly alternative to traditional petroleum-based plastics, and is therefore widely used in packaging materials, medical supplies, agricultural films, and other fields. Currently, PHA production processes are mainly divided into microbial fermentation and chemical synthesis methods. Microbial fermentation technology, through the selection and modification of strains and the optimization of fermentation techniques, has significantly reduced the complexity and cost of downstream processing, thus becoming the mainstream production process for PHA.
[0003] In the process of producing PHA by microbial fermentation, it is necessary to extract the cells from the fermentation broth and then use enzymatic hydrolysis and other methods to break the cell walls, thereby releasing PHA for further extraction and purification.
[0004] CN221117457U discloses a device for PHA extraction, comprising: a Z1 separation module; a Z2 enzymatic hydrolysis and cell disruption module; a Z3 extraction module; and a Z4 PHA post-processing module. The separation module includes a flocculation tank and a diaphragm plate and frame filter press; the enzymatic hydrolysis and cell disruption module includes an enzyme mixing tank, a primary homogenizing emulsification pump, and an enzymatic hydrolysis and cell disruption tank; the extraction module includes a secondary homogenizing emulsification pump and a sedimentation separation tank; and the PHA post-processing module includes a spray drying tank. This invention employs high-pressure diaphragm plate and frame filtration after flocculation, followed by a two-stage pipeline homogenizing emulsification process, which improves extraction efficiency and simplifies the entire process, making it particularly suitable for large-scale industrial production.
[0005] However, current PHA fermentation broth systems require mixing and heating in a mixing tank, which suffers from slow heating rates, high steam energy consumption, numerous equipment requirements, and high investment costs. Furthermore, it is highly dependent on stirring devices. Additionally, slow heating in the mixing tank has minimal impact on cell membranes, thus relying almost entirely on enzymatic hydrolysis to release PHA, resulting in a slow hydrolysis rate. Therefore, it is necessary to develop an enzymatic hydrolysis system capable of rapid heating and homogenization to improve hydrolysis efficiency. Summary of the Invention
[0006] To address the aforementioned technical problems, this invention provides an enzymatic hydrolysis system for rapid heating and homogenization of PHA. The system utilizes a steam ejector to rapidly heat the fermentation broth, followed by homogenization using a homogenizer. This improves both the heating rate and the hydrolysis rate, while simultaneously saving steam and reducing reliance on stirring devices.
[0007] To achieve the above objectives, this utility model provides an enzymatic hydrolysis system for rapid heating and homogenization of PHA, wherein the raw material storage tank is connected to a steam ejector, the steam ejector is connected to a homogenizer, the homogenizer is connected to an enzymatic hydrolysis tank, and the steam ejector is connected to a steam generator pipeline.
[0008] Preferably, the homogenizer is connected to both the enzyme storage tank and the steam generator pipeline.
[0009] Preferably, the steam generator is connected to the condensate storage tank pipeline.
[0010] Preferably, the enzymatic hydrolysis tank is equipped with a stirrer, a silencer, and a rotating spray ball.
[0011] Preferably, the stirrer is connected to the electrode.
[0012] More preferably, the silencing device is connected to the steam generator pipeline.
[0013] More preferably, the rotating spray ball is connected to the defoamer storage tank pipeline.
[0014] More preferably, there are two rotating spray balls.
[0015] Preferably, the enzymatic hydrolysis vessel is provided with an auxiliary agent inlet and a liquid replenishment inlet at the top.
[0016] The beneficial effects of this utility model are as follows:
[0017] 1. Pre-treatment of the fermentation broth using a steam ejector increases the heating area of the fermentation broth, thereby rapidly raising the temperature of the PHA fermentation broth. During this process, the cells in the fermentation broth are rapidly heated, and the cell membranes rupture due to thermal shock, achieving partial cell wall disruption. This increases the cell wall disruption speed, reduces the amount of enzyme preparation used and steam consumption, and thus saves production costs.
[0018] 2. A homogenizer is used to mix and homogenize the heated fermentation broth with the enzyme preparation. The mixture is then transferred to an enzymatic hydrolysis tank for further enzymatic hydrolysis. This accelerates the mixing speed of the enzyme preparation and fermentation broth, reduces reliance on the stirring device in the hydrolysis tank, ensures thorough mixing of the enzyme preparation and fermentation broth, and improves hydrolysis efficiency. Furthermore, it reduces the requirements for the stirring device in the hydrolysis tank, saving on equipment investment.
[0019] 3. This utility model realizes an integrated media process of mixing, heating, homogenization and cell wall breaking, optimizes the process route of PHA fermentation broth cell wall breaking, and has the advantages of fast heating speed, short production time, high reaction rate, low energy consumption and low cost, and can be applied to large-scale industrial production. Attached Figure Description
[0020] Figure 1This is a schematic diagram of the overall structure of this utility model. In the figure, 1 is an enzymatic hydrolysis tank, 2 is a stirrer, 3 is a silencer, 4 is a rotating spray ball, 5 is a motor, 6 is a defoamer storage tank, 7 is a raw material storage tank, 8 is a condensate storage tank, 9 is a steam generator, 10 is a steam injector, 11 is a homogenizer, 12 is an auxiliary agent inlet, 13 is a liquid replenishment port, and 14 is an enzyme storage tank. Detailed Implementation
[0021] The technical solution of this utility model will be further explained below with reference to the accompanying drawings and specific embodiments. It is worth noting that the following embodiments are only preferred embodiments of this utility model and should not be construed as limiting this utility model. The protection scope of this utility model should be determined by the content of the claims. Modifications or substitutions made by those skilled in the art to the technical solution of this utility model without creative effort all fall within the protection scope of this utility model.
[0022] Example 1
[0023] like Figure 1 As shown, an enzymatic hydrolysis system for rapid heating and homogenization of PHA is provided, wherein the raw material storage tank 7 is connected to the steam ejector 10, the steam ejector 10 is connected to the homogenizer 11, the homogenizer 11 is connected to the enzymatic hydrolysis tank 1, and the steam ejector 10 is connected to the steam generator 9 via pipeline.
[0024] Preferably, the homogenizer 11 is connected to the enzyme storage tank 14 and the steam generator 10 via pipelines.
[0025] Preferably, the steam generator 9 is connected to the condensate storage tank 8 via pipeline.
[0026] Preferably, the enzymatic hydrolysis tank 1 is equipped with a stirrer 2, a silencer 3, and a rotating spray ball 4.
[0027] More preferably, the stirrer 2 is connected to the electrode 5.
[0028] More preferably, the silencing device 3 is connected to the steam generator 9 via pipeline.
[0029] More preferably, the rotating spray ball 4 is connected to the defoamer storage tank 6 via pipeline.
[0030] More preferably, there are two rotating spray balls 4.
[0031] Preferably, the upper part of the enzymatic hydrolysis tank 1 is provided with an auxiliary agent inlet 12 and a liquid replenishment inlet 13.
[0032] Example 2
[0033] The PHA fermentation broth is transported from the raw material storage tank 7 to the steam ejector 10 via pipeline for rapid heating. The heated fermentation broth then enters the homogenizer 11. Simultaneously, the valve on the pipeline is opened to transport the enzyme from the enzyme storage tank 14 to the homogenizer 11, where it is uniformly mixed with the PHA fermentation broth. The homogenized fermentation broth is then transported through the pipeline to the enzymatic hydrolysis tank 1 for enzymatic hydrolysis. After enzymatic hydrolysis, the broth is discharged through the outlet at the bottom of the enzymatic hydrolysis tank 1 and proceeds to the next process.
[0034] During the enzymatic hydrolysis process, the homogenized fermentation broth in the enzymatic hydrolysis tank 1 is further stirred and mixed by the stirrer 2 driven by the motor 5, so that the fermentation broth is uniformly enzymatically hydrolyzed. At the same time, the valve on the pipeline is opened to transport the defoamer in the defoamer storage tank 6 to the enzymatic hydrolysis tank 1, and then sprayed on the surface of the fermentation broth by the rotating spray ball 4 to defoam.
[0035] The noise generated in the enzymatic hydrolysis tank 1 during the enzymatic hydrolysis process is treated by the silencing device 3 to reduce the noise. The silencing device 3 is connected to the steam generator 9 pipeline, and steam is continuously supplied to the silencing device 3 to achieve noise reduction and also to heat the fermentation liquid in the enzymatic hydrolysis tank 1.
[0036] The steam generator 9 is connected to the condensate storage tank 8 to collect and store the steam condensate.
[0037] The enzymatic hydrolysis tank 1 is equipped with an auxiliary agent inlet 12 and a liquid replenishment inlet. Raw materials are delivered into the enzymatic hydrolysis tank 1 according to the needs of enzymatic hydrolysis to improve the efficiency of enzymatic hydrolysis.
Claims
1. An enzymatic hydrolysis system for rapid heating and homogenization of PHA, characterized in that: The raw material storage tank (7) is connected to the steam ejector (10), the steam ejector (10) is connected to the homogenizer (11), the homogenizer (11) is connected to the enzymatic hydrolysis tank (1), and the steam ejector (10) is connected to the steam generator (9) pipeline.
2. The enzymatic hydrolysis system for rapid heating and homogenization of PHA according to claim 1, characterized in that: The homogenizer (11) is connected to the enzyme storage tank (14) and the steam ejector (10) pipelines respectively.
3. The enzymatic hydrolysis system for rapid heating and homogenization of PHA according to claim 1, characterized in that: The steam generator (9) is connected to the condensate storage tank (8) via pipeline.
4. The enzymatic hydrolysis system for rapid heating and homogenization of PHA according to claim 1, characterized in that: The enzymatic hydrolysis tank (1) is equipped with a stirrer (2), a silencer (3), and a rotating spray ball (4).
5. The enzymatic hydrolysis system for rapid heating and homogenization of PHA according to claim 4, characterized in that: The stirrer (2) is connected to the electrode (5).
6. The enzymatic hydrolysis system for rapid heating and homogenization of PHA according to claim 4, characterized in that: The silencing device (3) is connected to the steam generator (9) via pipeline.
7. The enzymatic hydrolysis system for rapid heating and homogenization of PHA according to claim 4, characterized in that: The rotating spray ball (4) is connected to the defoamer storage tank (6) via pipeline.
8. The enzymatic hydrolysis system for rapid heating and homogenization of PHA according to claim 1, characterized in that: The enzymatic hydrolysis vessel (1) is provided with an auxiliary agent inlet (12) and a liquid replenishment inlet (13) at the top.