Nucleic acid extraction and colloidal gold detection all-in-one machine

Abstract

The embodiment of the utility model provides a nucleic acid extraction and colloidal gold detection all-in-one machine which is beneficial to reducing artificial participation and improving the working efficiency of nucleic acid extraction and detection. The nucleic acid extraction and colloidal gold detection all-in-one machine comprises a bottom plate; the reagent assembly and the sampling tube assembly are both arranged on the bottom plate, the sampling tube assembly is used for containing a sample, and the reagent assembly comprises a kit, a deep hole plate and a colloidal gold card box; the nucleic acid extraction assembly is movably connected to the bottom plate in the first direction and can stretch into the deep hole plate or be separated from the deep hole plate in the second direction, the second direction is the thickness direction of the bottom plate, and the first direction is perpendicular to the second direction; the liquid transferring assembly is configured to transfer liquid among the sampling tube assembly, the kit, the deep hole plate and the colloidal gold card box.

Description

Technical Field

[0001] This application relates to the field of forensic biological testing technology, and in particular to an integrated machine for nucleic acid extraction and colloidal gold detection. Background Technology

[0002] In recent years, with the rapid development of molecular biology, nucleic acid-based molecular diagnostics and detection technologies have played an increasingly important role in many fields. Currently, magnetic bead extraction is commonly used for nucleic acid extraction.

[0003] In related technologies, after nucleic acid extraction is completed, the target sequence of the nucleic acid needs to be detected and analyzed. However, currently, the extracted nucleic acid needs to be sent for analysis manually, which makes the entire workflow time-consuming, labor-intensive, and inefficient. Utility Model Content

[0004] This application provides an integrated machine for nucleic acid extraction and colloidal gold detection, which helps to reduce manual intervention and improve the efficiency of nucleic acid extraction and detection.

[0005] A nucleic acid extraction and colloidal gold detection integrated machine includes: a base plate; a reagent assembly and a sampling tube assembly, both disposed on the base plate, wherein the sampling tube assembly is used to contain samples, and the reagent assembly includes a reagent kit, a deep-well plate, and a colloidal gold cartridge; a nucleic acid extraction assembly, movably connected to the base plate along a first direction and capable of extending into or detaching from the deep-well plate in a second direction, wherein the second direction is the thickness direction of the base plate, and the first direction and the second direction are perpendicular; and a pipetting assembly configured to transfer liquids between the sampling tube assembly, the reagent kit, the deep-well plate, and the colloidal gold cartridge.

[0006] The nucleic acid extraction and colloidal gold detection integrated machine of this application has a sampling tube assembly, reagent kit, deep-well plate, and colloidal gold cartridge mounted on a base plate. It also includes a nucleic acid extraction assembly and a pipetting assembly that can move relative to the base plate. In this way, the nucleic acid extraction assembly and the pipetting assembly work together to automate the extraction, purification, and detection of nucleic acids. This helps reduce manual intervention, shortens the time, and improves the efficiency of nucleic acid testing. Attached Figure Description

[0007] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0008] Figure 1This is a schematic diagram of the structure of a nucleic acid extraction and colloidal gold detection integrated machine according to an embodiment of this application;

[0009] Figure 2 This is a schematic diagram of the structure of a colloidal gold card box according to an embodiment of this application;

[0010] Figure 3 This is a schematic diagram of the structure of a nucleic acid extraction component according to an embodiment of this application;

[0011] Figure 4 This is a schematic diagram of the structure of a pipetting assembly according to an embodiment of this application;

[0012] Figure 5 This is a schematic diagram of the structure of a pipetting unit according to an embodiment of this application;

[0013] Figure 6 This is a cross-sectional schematic diagram of the first rack according to an embodiment of this application.

[0014] Explanation of icon numbers:

[0015] 10- Nucleic Acid Extraction and Colloidal Gold Detection Integrated Machine;

[0016] 100-Base plate, 110-Connecting plate, 200-Reagent assembly, 210-Reagent kit, 220-Deep well plate, 230-Colloidal gold card holder, 231-Card holder, 232-Colloidal gold detection card, 300-Sampling tube assembly, 310-Sampling tube, 400-Nucleic acid extraction assembly, 410-Mounting bracket, 420-Magnetic rod connecting plate, 430-Magnetic rod holder, 440-Magnetic rod, 450-Magnetic sleeve connecting plate, 460-Magnetic sleeve holder, 500-Pipette assembly, 510-Outer shell, 52 0-Pipette unit, 521-Tip connector, 522-Third drive structure, 5221-Second motor, 5222-Second gear, 523-Fourth drive structure, 5231-First support, 5232-First motor, 5233-First gear, 5234-First rack, 5234a-Wiring cavity, 5234b-Guide surface, 5235-Position sensor, 600-First drive structure, 700-Second drive structure, 800-Tip holder, 810-TIP tip. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0018] In the description of this application, it should be understood that if terms such as "upper," "lower," "left," and "right" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, they are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the accompanying drawings are only for illustrative purposes and should not be construed as limiting this patent. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0019] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as implying or suggesting relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified. "And / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A alone, A and B simultaneously, or B alone. The character " / " generally indicates that the preceding and following related objects are in an "or" relationship.

[0020] In the description of this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0021] Nucleic acid extraction is an important part of molecular diagnostics, and magnetic bead extraction is a common method. In this method, cell and tissue samples are lysed using a lysis buffer. The free nucleic acid molecules released from the sample are specifically adsorbed onto the surface of the magnetic beads, while proteins and other impurities remain in the solution. The adsorbed nucleic acid beads are then sequentially transferred to washing and elution buffers to obtain pure nucleic acid.

[0022] After nucleic acid purification, it still needs to be detected and analyzed. One commonly used detection technique is immunogold assay, which is a novel immunolabeling technique that uses colloidal gold as a tracer for antigen-antibody interactions. It is widely used in fields such as immunology, histology, pathology, and cell biology.

[0023] In related technologies, the purified nucleic acid is usually sent for testing manually, which makes the whole process time-consuming, labor-intensive, and inefficient.

[0024] Based on the above problems, this application proposes a nucleic acid extraction colloidal gold detection integrated machine 10, which helps to reduce manual intervention and improve the efficiency of nucleic acid detection.

[0025] like Figure 1 As shown, the nucleic acid extraction and colloidal gold detection integrated machine 10 includes a base plate 100, a reagent assembly 200, a sampling tube assembly 300, a nucleic acid extraction assembly 400, and a pipetting assembly 500. The reagent assembly 200 and the sampling tube assembly 300 are both mounted on the base plate 100. The sampling tube assembly 300 is used to hold samples. The reagent assembly 200 includes a reagent kit 210, a deep-well plate 220, and a colloidal gold cartridge 230. The nucleic acid extraction assembly 400 is movably connected to the base plate 100 along a first direction X. The nucleic acid extraction assembly 400 can extend into or detach from the deep-well plate 220 in a second direction Z, where Z is the thickness direction of the base plate 100. The first direction X and the second direction Z are perpendicular. The pipetting assembly 500 is configured to transfer liquids between the sampling tube assembly 300, the reagent kit 210, the deep-well plate 220, and the colloidal gold cartridge 230.

[0026] In this application, the base plate 100 serves as the substrate of the nucleic acid extraction colloidal gold detection integrated machine 10. The nucleic acid extraction colloidal gold detection integrated machine 10 may also include an upper shell, which covers the base plate 100 to form a receiving cavity for accommodating the reagent assembly 200, the sampling tube assembly 300, the nucleic acid extraction assembly 400, and the pipetting assembly 500. This avoids contamination from the external environment and improves the reliability of the detection. Optionally, an ultraviolet disinfection lamp and an experimental lighting lamp may also be installed inside the receiving cavity, thereby further improving the convenience of nucleic acid extraction and detection.

[0027] The reagent assembly 200 includes a kit 210, a deep-well plate 220, and a colloidal gold cartridge 230. The kit 210 provides various reagents required for nucleic acid extraction, such as lysis reagents, elution reagents, and washing reagents. The deep-well plate 220 is used to hold the sample and required reagents during nucleic acid extraction. The colloidal gold cartridge 230 is used for the detection and analysis of the extracted nucleic acid. Figure 2 As shown, the colloidal gold card box 230 includes multiple colloidal gold detection cards 232. The colloidal gold detection cards 232 can rapidly detect the target nucleic acid sequence in the extracted nucleic acid using immunolabeling technology with colloidal gold as a tracer marker.

[0028] The sampling tube assembly 300 is used to contain the initial sample to be extracted after sampling. The sampling tube assembly 300 may include multiple sampling tubes 310 arranged in an array. The nucleic acid extraction assembly 400 is used to extract nucleic acid from the sample in the deep well plate 220 after adding nucleic acid extraction reagent. The deep well plate 220 may be a 24-well plate, a 96-well plate, or a deep well plate with a special number of wells, etc., and this application does not limit it.

[0029] The pipetting assembly 500 is configured to transfer liquids between the sampling tube assembly 300, the reagent kit 210, the deep-well plate 220, and the colloidal gold cartridge 230. Thus, the pipetting assembly 500, in conjunction with the nucleic acid extraction assembly 400, enables a fully automated process for nucleic acid extraction and detection. Specifically, firstly, an initial sample, such as blood, urine, or saliva, is placed in the sampling tube assembly 300. Then, the pipetting assembly 500 transfers the sample into the deep-well plate 220. Following the extraction steps, the pipetting assembly 500 sequentially transfers reagents from the reagent kit 210 into the corresponding wells of the deep-well plate 220, allowing the nucleic acid extraction assembly 400 to extract and purify the nucleic acid within the deep-well plate 220. Finally, the pipetting assembly 500 transfers the extracted nucleic acid to the colloidal gold cartridge 230 for rapid detection.

[0030] The nucleic acid extraction and colloidal gold detection integrated machine 10 of this application includes a sampling tube assembly 300, a reagent kit 210, a deep-well plate 220, and a colloidal gold cartridge 230 mounted on a base plate 100. It also includes a nucleic acid extraction assembly 400 and a pipetting assembly 500, which are movable relative to the base plate 100. Thus, the nucleic acid extraction assembly 400 and the pipetting assembly 500 work together to automatically complete the extraction, purification, and detection of nucleic acids without manual intervention. This reduces manual intervention, shortens the time required, and improves the efficiency of nucleic acid detection.

[0031] In some embodiments, such as Figure 1 As shown, the pipetting assembly 500 is connected to the base plate 100 via a first drive structure 600, which drives the pipetting assembly 500 to move along a first direction X. Controlling the movement of the pipetting assembly 500 along the first direction X via the first drive structure 600 improves the intelligence and automation of the pipetting assembly 500's movement. Optionally, the first drive structure 600 can be an electric slide.

[0032] In some embodiments, such as Figure 1 and Figure 3As shown, the nucleic acid extraction component 400 is connected to the base plate 100 via a second driving structure 700, which drives the nucleic acid extraction component 400 to move along a first direction X. Controlling the movement of the nucleic acid extraction component 400 along the first direction X via the second driving structure 700 improves the intelligence and automation of its movement. Optionally, the second driving structure 700 can be, for example, a synchronous belt drive structure. Optionally, a connecting plate 110 is also provided between the second driving structure 700 and the base plate 100, with the second driving structure 700 mounted on the connecting plate 110, thereby improving the reliability and accuracy of the nucleic acid extraction component 400's operation.

[0033] Since both the nucleic acid extraction component 400 and the pipetting component 500 can move along the first direction X, this avoids the nucleic acid extraction component 400 from blocking the movement of the pipetting component 500. Thus, the ease of operation of the pipetting component 500 can be improved while ensuring the miniaturization of the equipment.

[0034] In some embodiments, such as Figure 1 and Figure 4 As shown, the base plate 100 is also provided with a pipette tip holder 800, which is used to hold TIP pipette tips 810. TIP pipette tips 810 refer to pipette tips, which can be used to store and dispense solutions.

[0035] Furthermore, such as Figure 1 , Figure 4 and Figure 5 As shown, the pipetting assembly 500 includes a housing 510 and a plurality of pipetting units 520. Parts of the pipetting units 520 are located inside the housing 510. The pipetting unit 520 includes a tip connector 521 for connecting to a TIP tip 810. The tip connector 521 is capable of linear movement relative to the base plate 100 in the third direction Y and the second direction Z. The third direction Y is perpendicular to both the second direction Z and the first direction X.

[0036] In this embodiment, the pipetting assembly 500 includes a housing 510 and multiple pipetting units 520. The housing 510 can protect part of the structure of the pipetting unit 520, thereby improving the reliability and lifespan of the pipetting unit 520. The pipetting unit 520 includes a tip connector 521, which can change position in three directions, allowing each tip connector 521 to reach any position on the base plate 100. The tip connector 521 can be loaded with a TIP tip 810, and then the pipetting operation can be completed through the TIP tip 810. This helps to improve the intelligence and automation of the pipetting assembly 500, reduce manual intervention and time costs, and improve work efficiency.

[0037] Optionally, the number of pipetting units 520 can be 2, 3, 4, etc. Multiple pipetting units 520 can be arranged in a single row along the third direction Y. In this way, the spacing between two adjacent pipetting units 520 can be adjusted to realize the synchronous transfer of liquid to multiple orifices in a single row, thereby further improving work efficiency.

[0038] In some embodiments, such as Figure 4 and Figure 5 As shown, the pipetting unit 520 also includes a third driving structure 522 disposed inside the housing 510 and a fourth driving structure 523 connected to the output end of the third driving structure 522. The pipette tip connector 521 is connected to the output end of the fourth driving structure 523. The third driving structure 522 is used to drive the pipette tip connector 521 to move along the third direction Y, and the fourth driving structure 523 is used to drive the pipette tip connector 521 to move along the second direction Z.

[0039] In this embodiment, the pipetting unit 520 further includes a third driving structure 522 and a fourth driving structure 523. The third driving structure 522 drives the pipette tip connector 521 to move along the third direction Y, and the fourth driving structure 523 drives the pipette tip connector 521 to move along the second direction Z. Thus, in conjunction with the movement of the pipetting assembly 500 in the first direction X, the pipette tip connector 521 can achieve positional changes in three directions. This improves the intelligence and automation of the pipetting assembly 500's operation, reduces labor and time costs, and increases work efficiency.

[0040] Optionally, both the third drive structure 522 and the fourth drive structure 523 can be rack and pinion drive structures. This simplifies the structure, reduces costs, and improves the reliability and stability of the drive.

[0041] In some embodiments, such as Figures 4 to 6 As shown, the fourth drive structure 523 includes a first bracket 5231 disposed in the housing 510, a first motor 5232 connected to the first bracket 5231, a first gear 5233 connected to the output end of the first motor 5232, and a first rack 5234 meshing with the first gear 5233. The first bracket 5231 is provided with a cavity extending along the second direction Z. At least a portion of the first rack 5234 passes through the cavity. The end of the first rack 5234 is connected to the suction head connector 521. A wiring cavity 5234a is provided inside the first rack 5234.

[0042] This embodiment presents a specific construction of the fourth drive structure 523. Through the aforementioned gear and rack drive structure, the first rack 5234 can be driven to reciprocate along the second direction Z, thereby realizing the reciprocating motion of the suction head connector 521 in the second direction Z, which helps to improve the reliability and stability of the drive. In addition, a wiring cavity 5234a is provided inside the first rack 5234, which also helps to improve the convenience of wiring.

[0043] It is easy to understand, such as Figures 4 to 6 As shown, the third drive structure 522 may include a second motor 5221 connected to the first bracket 5231, a second gear 5222 connected to the output end of the second motor 5221, and a second rack (not shown) meshing with the second gear 5222. The second rack is fixedly connected to the housing 510, that is, the second gear 5222 is the output end of the third drive structure 522, thereby causing the second gear 5222 to drive the first bracket 5231 to move relative to the housing 510 in the third direction Y, thereby realizing the reciprocating motion of the suction head connector 521 in the third direction Y.

[0044] In some embodiments, such as Figure 4 and Figure 5 As shown, the fourth drive structure 523 also includes a position sensor 5235 disposed on the first bracket 5231 and / or the suction head connector 521. The position sensor 5235 is used to detect the position of the suction head connector 521 relative to the first bracket 5231 in the second direction Z. By setting the position sensor 5235, the position of the suction head connector 521 in the second direction Z can be obtained. This position can be the initial position when the suction head connector 521 and the first bracket 5231 come into contact, or the end position when the suction head connector 521 moves to its maximum stroke. This helps to improve the accuracy and reliability of the movement of the suction head connector 521. The position sensor 5235 can be, for example, a Hall effect proximity switch, a photoelectric proximity switch, etc., and this application does not limit it.

[0045] In some embodiments, such as Figure 5 and Figure 6 As shown, the first rack 5234 is provided with a guide surface 5234b for engaging with the inner wall of the cavity. By providing the guide surface 5234b, the smoothness and reliability of the movement of the first rack 5234 can be improved.

[0046] In some embodiments, such as Figure 2 As shown, the colloidal gold card holder 230 includes a card holder 231 and a plurality of colloidal gold test cards 232 disposed on the card holder 231. For example, the card holder 231 may be provided with card slots, and the plurality of colloidal gold test cards 232 are respectively snapped into the card slots. This can improve the reliability of the colloidal gold test cards 232 being fixed.

[0047] Furthermore, multiple colloidal gold test cards 232 are arranged in the same layer and in an array. This improves the convenience of testing and reduces the manufacturing cost of the colloidal gold card holder 230.

[0048] In other embodiments, such as Figure 2 As shown, along the first direction X, two adjacent colloidal gold test cards 232 are arranged at intervals on the same layer. Along the third direction Y, two adjacent colloidal gold test cards 232 are staggered along the second direction Z, and the orthographic projections of two adjacent colloidal gold test cards 232 on the card holder 231 overlap. This arrangement reduces the space occupied by multiple colloidal gold test cards 232 in the third direction Y while ensuring that each colloidal gold test card 232 can perform normal testing, thus improving space utilization and reducing the volume of the colloidal gold card holder 230. Therefore, more colloidal gold card holders 230 can be set, improving testing efficiency.

[0049] In some embodiments, such as Figure 1 and Figure 3 As shown, the nucleic acid extraction component 400 includes a mounting bracket 410, a magnetic rod connecting plate 420, multiple magnetic rod supports 430, magnetic rods 440, a magnetic sleeve connecting plate 450, multiple magnetic sleeve supports 460, and magnetic sleeves (not shown in the figure). The magnetic rod connecting plate 420 is movably mounted on the mounting bracket 410 along the second direction Z. The multiple magnetic rod supports 430 are connected to the magnetic rod connecting plate 420 and arranged at intervals along the first direction X. Each magnetic rod support 430 has multiple magnetic rods 440 connected in a single column along the third direction Y. The magnetic sleeve connecting plate 450 is located below the magnetic rod connecting plate 420 and is movably mounted on the mounting bracket 410 along the second direction Z. The multiple magnetic sleeve supports 460 are connected to the magnetic sleeve connecting plate 450 and are arranged opposite to the multiple magnetic rod supports 430. Each magnetic sleeve support 460 has multiple magnetic sleeves connected in a single column along the third direction Y. The magnetic rods 440 and magnetic sleeves correspond one-to-one.

[0050] This embodiment presents a specific structure for the nucleic acid extraction component 400. A magnetic rod connecting plate 420 is movably mounted on a mounting bracket 410 along the second direction Z. The magnetic rod connecting plate 420 can be connected to the mounting bracket 410 via a fifth driving structure 480, which can be a ball screw drive structure. Similarly, a magnetic sleeve connecting plate 450 is located below the magnetic rod connecting plate 420 and is movably mounted on the mounting bracket 410 along the second direction Z. The magnetic sleeve connecting plate 450 can be connected to the mounting bracket 410 via a sixth driving structure 490, which can also be a ball screw drive structure. Thus, the magnetic sleeve and magnetic rod 440 can be combined or separated. When combined, the magnetic beads are attracted to the outer surface of the magnetic sleeve by the magnetic rod 440; when separated, the magnetic beads detach from the magnetic sleeve.

[0051] Furthermore, multiple magnetic rod supports 430 are connected to magnetic rod connecting plates 420 and arranged at intervals along the first direction X. Multiple magnetic rods 440 are connected in a single column along the third direction Y. Multiple magnetic sleeve supports 460 are connected to magnetic sleeve connecting plates 450 and are positioned opposite to the multiple magnetic rod supports 430. Multiple magnetic sleeve supports 460 are connected in a single column along the third direction Y, with each magnetic rod 440 and magnetic sleeve corresponding to the others. In this way, the multiple magnetic rods 440 in each column and the corresponding column of magnetic sleeves can complete the nucleic acid extraction or purification work in one column of wells on the deep-well plate 220. Therefore, by reasonably setting the spacing between two adjacent magnetic rod supports 430, the nucleic acid extraction component 400 can be adapted to deep-well plates 220 with a specific number of columns, thereby improving the versatility and adaptability of the nucleic acid extraction component 400. For example, the number of magnetic rods 440 and magnetic sleeves in each column can be 8, and the deep hole plate 220 can be a specially made deep hole plate with 5×8, 8×8, 9×8, 10×8 or other hole numbers.

[0052] In some embodiments, the nucleic acid extraction colloidal gold detection integrated machine 10 further includes a heating element (not shown) and a heat insulation element (not shown) disposed on the base plate 100. The heating element is located between the deep well plate 220 and the base plate 100, and the heat insulation element is located between the heating element and the base plate 100. By providing the heating element, the deep well plate 220 can be heated, thereby improving extraction efficiency. The heating element can be, for example, a heating wire, a heating element, a PTC heater, etc. By providing the heat insulation element, heat transfer to the base plate 100 can be prevented, avoiding burns to personnel from the nucleic acid extraction colloidal gold detection integrated machine 10, thereby improving equipment safety. The heat insulation element can be, for example, aerogel, asbestos board, ceramic plate, etc.

[0053] The above are merely preferred embodiments of this application and are not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A nucleic acid extraction and colloidal gold detection integrated machine, characterized in that, include: Base plate; Both the reagent assembly and the sampling tube assembly are mounted on the base plate. The sampling tube assembly is used to hold the sample. The reagent assembly includes a reagent kit, a deep well plate, and a colloidal gold card holder. A nucleic acid extraction component is movably connected to the base plate along a first direction and can extend into or detach from the deep well plate in a second direction, wherein the second direction is the thickness direction of the base plate, and the first direction and the second direction are perpendicular. A pipetting assembly configured to transfer liquids between the sampling tube assembly, the reagent kit, the deep well plate, and the colloidal gold cartridge.

2. The integrated nucleic acid extraction and colloidal gold detection machine according to claim 1, characterized in that, The pipetting assembly is connected to the base plate via a first driving structure, the first driving structure being used to drive the pipetting assembly to move along the first direction; The nucleic acid extraction component is connected to the base plate via a second driving structure, which drives the nucleic acid extraction component to move along the first direction.

3. The integrated nucleic acid extraction and colloidal gold detection machine according to claim 1, characterized in that, The base plate is also provided with a suction head holder, which is used to hold TIP suction heads; The pipetting assembly includes a housing and a plurality of pipetting units, with portions of each pipetting unit located inside the housing. Each pipetting unit includes a tip connector for connecting to a TIP tip. The tip connector is capable of linear movement relative to the base plate in a second direction and a third direction, with the third direction being perpendicular to the first direction and the second direction.

4. The integrated nucleic acid extraction and colloidal gold detection machine according to claim 3, characterized in that, The pipetting unit further includes a third driving structure disposed inside the housing and a fourth driving structure connected to the output end of the third driving structure. The pipette tip connector is connected to the output end of the fourth driving structure. The third driving structure is used to drive the pipette tip connector to move along the third direction, and the fourth driving structure is used to drive the pipette tip connector to move along the second direction.

5. The nucleic acid extraction and colloidal gold detection integrated machine according to claim 4, characterized in that, Both the third and fourth drive structures are gear and rack drive structures.

6. The nucleic acid extraction and colloidal gold detection integrated machine according to claim 4, characterized in that, The fourth drive structure includes a first bracket disposed within the housing, a first motor connected to the first bracket, a first gear connected to the output end of the first motor, and a first rack meshing with the first gear. The first bracket has a cavity extending along the second direction, at least a portion of the first rack passes through the cavity, the end of the first rack is connected to the suction head connector, and a wiring cavity is provided inside the first rack.

7. The nucleic acid extraction and colloidal gold detection integrated machine according to claim 6, characterized in that, The fourth driving structure further includes a position sensor disposed on the first bracket and / or the suction head connector, the position sensor being used to detect the position of the suction head connector relative to the first bracket in the second direction; And / or, the first rack is provided with a guide surface for fitting against the inner wall of the cavity.

8. The integrated nucleic acid extraction and colloidal gold detection machine according to claim 1, characterized in that, The colloidal gold card box includes a card holder and a plurality of colloidal gold detection cards disposed on the card holder; The colloidal gold test cards are arranged in the same layer and in an array; or, along the first direction, two adjacent colloidal gold test cards are arranged at intervals in the same layer, and along the third direction, two adjacent colloidal gold test cards are staggered in the second direction, and the orthogonal projections of two adjacent colloidal gold test cards on the card holder overlap, and the third direction is perpendicular to the first direction and the second direction.

9. The integrated nucleic acid extraction and colloidal gold detection machine according to claim 1, characterized in that, The nucleic acid extraction component includes: Mounting bracket; The system includes a magnetic rod connecting plate, multiple magnetic rod supports, and magnetic rods. The magnetic rod connecting plate is movably mounted on the mounting bracket along the second direction. The multiple magnetic rod supports are connected to the magnetic rod connecting plate and are arranged at intervals along the first direction. Each magnetic rod support has multiple magnetic rods connected in a single row along a third direction, which is perpendicular to both the first and second directions. The system includes a magnetic sleeve connecting plate, multiple magnetic sleeve brackets, and magnetic sleeves. The magnetic sleeve connecting plate is located below the magnetic rod connecting plate and is movably mounted on the mounting bracket along the second direction. The multiple magnetic sleeve brackets are connected to the magnetic sleeve connecting plate and are arranged opposite to the multiple magnetic rod brackets. Each magnetic sleeve bracket has multiple magnetic sleeves connected in a single row along the third direction. The magnetic rods and magnetic sleeves correspond one-to-one.

10. The integrated nucleic acid extraction and colloidal gold detection machine according to claim 1, characterized in that, The nucleic acid extraction colloidal gold detection integrated machine also includes a heating element and a heat insulation element disposed on the base plate. The heating element is located between the deep well plate and the base plate, and the heat insulation element is located between the heating element and the base plate.

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