Auxiliary device for constant-temperature fluorescent quantitative reagent detection

Abstract

The utility model discloses an auxiliary device for constant-temperature fluorescent quantitative reagent detection, and relates to the technical field of detection devices. A shell is fixedly connected to the top face of the bottom plate, a toothed plate is fixedly connected to the upper portion of the inner side wall of the shell, a movable frame is arranged below the toothed plate, a transverse shaft is arranged above the toothed plate, a gear and a worm wheel are fixedly arranged on the side wall of the transverse shaft in a sleeved mode, the gear corresponds to and is meshed with the toothed plate, and a motor is arranged on one side of the movable frame. The output shaft end of the motor is fixedly connected with a worm, the worm is meshed with the worm gear, an electric push rod is arranged in the middle of the bottom surface of the movable frame, a convex plate is fixedly connected to the inner bottom wall of the shell, a placement frame is detachably arranged above the convex plate, a placement groove is formed in the top surface of the placement frame in a penetrating mode, and a detection pipe can be taken and placed in the placement groove. The device is high in detection efficiency, is not in contact with the detection tube, avoids pollution to a sample, and ensures the cleanliness.

Description

TECHNICAL FIELD

[0001] The utility model relates to detection device technical field, concretely is a kind of auxiliary device for constant temperature fluorescent quantitative reagent detection. BACKGROUND

[0002] Constant temperature fluorescent quantitative reagent is the fluorescence signal that is emitted in PCR reaction process using fluorescent dye or fluorescently labeled primer, with the PCR cycle, the intensity of fluorescence signal is directly proportional to the number of template DNA, by monitoring these fluorescence signal changes, the process of PCR reaction can be monitored in real time, so that the nucleic acid content in sample is accurately calculated.

[0003] The current constant temperature fluorescent quantitative reagent needs to add reagent liquid to sample before detection, this process needs to ensure that sample is not contaminated, and adding manually by staff can easily contact with pipe wall, and then cause pollution, affect the accuracy of detection, to solve the above problems, the inventor proposes a kind of constant temperature fluorescent quantitative reagent detection auxiliary device for solving the above problems. UTILITY MODEL CONTENT

[0004] To solve the problem that the current constant temperature fluorescent quantitative reagent cannot effectively ensure that sample is not contaminated during detection, the utility model aims to provide a kind of constant temperature fluorescent quantitative reagent detection auxiliary device.

[0005] To solve the above technical problems, the utility model adopts the following technical scheme: a kind of constant temperature fluorescent quantitative reagent detection auxiliary device, including bottom plate, the top surface of the bottom plate is fixedly connected with shell, the inner side wall upper portion of the shell is fixedly connected with tooth plate, the lower portion of the tooth plate is provided with movable rack, the upper portion of the tooth plate is provided with horizontal shaft, the sidewall of the horizontal shaft is fixedly sleeved with gear and worm wheel, the gear is correspondingly engaged with tooth plate, one side of the movable rack is provided with motor, the output shaft end of the motor is fixedly connected with worm, the worm is engaged with worm wheel, the bottom surface middle portion of the movable rack is provided with electric push rod, the output shaft end of the electric push rod is fixedly connected with piston, the lower portion of the movable rack is provided with conical barrel, the piston is located in the barrel mouth of conical barrel, the inner bottom wall of the shell is fixedly connected with convex plate, the upper portion of the convex plate is detachably provided with placing rack, the top surface of the placing rack is provided with placing groove, the detection tube can be taken and placed in the placing groove.

[0006] Preferably, the side wall of the shell is hinged with an opening and closing door, and the opening and closing door corresponds to the placing rack, the end surface of the placing rack is fixedly connected with a convex disc, the bottom surface of the convex disc is provided with a clamping groove, the top surface of the convex plate is fixedly connected with a clamping rod on both sides, the clamping rod corresponds to and cooperates with the clamping groove, and the placing rack can be taken out by opening the opening and closing door. When the placing rack is installed, the clamping groove corresponds to the clamping rod, the placing rack is pressed downward, the clamping groove cooperates with the clamping rod, the installation of the placing rack is completed, and the placing rack can be taken down by reverse operation when disassembled, so that the detection tube can be taken out, and the detection tube is not contacted throughout the process, thereby avoiding pollution of the sample.

[0007] Preferably, the two side surfaces of the tooth plate are fixedly connected with sliding rails, the top surface of the movable frame is fixedly connected with a sliding block, the sliding block comprises a sliding groove, the sliding groove corresponds to and is slidingly connected with the sliding rail, the side wall of the horizontal shaft is rotatably connected with a vertical plate at both ends, and the bottom surface of the vertical plate is fixedly connected with the top surface of the sliding block. The motor is started, the worm is rotated under the action of the motor output shaft, the horizontal shaft drives the gear to rotate through the mutual meshing of the worm and the worm gear, the movable frame is translated through the mutual meshing of the gear and the tooth plate and the cooperation of the sliding rail and the sliding groove, and the position of the conical cylinder can be adjusted, so that the conical cylinder can drip reagent to different detection tubes.

[0008] Preferably, the side wall of the motor is provided with a base, the base is fixedly connected with the side wall of the movable frame, the side wall of the electric push rod is provided with a fixing seat, the fixing seat is fixedly connected with the bottom surface of the movable frame, the outer side wall of the conical cylinder is fixedly connected with a fixing rod, and the top end of the fixing rod is fixedly connected with the bottom surface of the movable frame. The motor is installed through the base, the electric push rod is started, the piston moves under the action of the electric push rod output shaft, so that the liquid in the conical cylinder is pushed down, the reagent is dripped from the conical cylinder, the top surface of the convex plate is fixedly connected with a convex seat on one side, the convex seat is embedded with an electric cylinder on the top surface, the output shaft end of the electric cylinder is fixedly connected with a lifting disc, and the lifting disc is placed with a reagent cup on the top surface. The electric cylinder is started, the lifting disc can drive the reagent cup to rise and fall under the action of the electric cylinder output shaft, when the conical cylinder needs to be installed with reagent, the conical cylinder corresponds to the reagent cup, the lifting disc drives the reagent cup to rise, so that the conical cylinder cooperates with the reagent cup, the piston is controlled to rise, the reagent in the reagent cup is sucked into the conical cylinder, and after completion, the lifting disc drives the reagent cup to fall, so that the movement of the conical cylinder is avoided.

[0009] Compared with the prior art, the beneficial effects of the utility model lie in that: a plurality of reagent tubes can be drip irrigated with reagents at one time, and the reagent tubes are taken and placed without contacting the detection tubes throughout the process, so that pollution of the sample is avoided, cleanliness is ensured, and when the conical cylinder needs to be loaded with reagents, the reagent cup can be lifted and lowered by the lifting disc, and under the action of the piston, the reagents in the reagent cup can be sucked into the conical cylinder, and after completion, the reagent cup can be caused to fall by the lifting disc, so as to avoid affecting the movement of the conical cylinder. BRIEF DESCRIPTION OF DRAWINGS

[0010] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, a brief introduction will be given below to the drawings needed to be used in the embodiments or the prior art description, and obviously, the drawings in the following description are only some embodiments of the present utility model, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0011] Figure 1 It is a whole structure schematic view of the present utility model.

[0012] Figure 2 It is a shell internal structure schematic view of the present utility model.

[0013] Figure 3 It is a placing rack structure schematic view of the present utility model.

[0014] Figure 4 It is a movable rack structure schematic view of the present utility model.

[0015] In the drawing: 1, bottom plate; 2, shell; 3, opening and closing door; 4, toothed plate; 5, slide rail; 6, convex plate; 7, clamping rod; 8, convex seat; 9, electric cylinder; 10, lifting disc; 11, reagent cup; 12, placing rack; 13, convex disc; 14, clamping groove; 15, placing groove; 16, detection tube; 17, movable rack; 18, sliding block; 19, sliding groove; 20, vertical plate; 21, horizontal shaft; 22, gear; 23, worm gear; 24, motor; 25, worm; 26, electric push rod; 27, piston; 28, conical cylinder; 29, fixed rod. DETAILED DESCRIPTION

[0016] The technical solutions in the embodiments of the present utility model will be described clearly and completely below in combination with the drawings in the embodiments of the present utility model, and obviously, the described embodiments are only some embodiments of the present utility model, rather than all the embodiments. Based on the embodiments in the present utility model, all the other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the present utility model.

[0017] Embodiment: as Figures 1-4The utility model provides a kind of auxiliary device for constant temperature fluorescent quantitative reagent detection, including bottom plate 1, the top surface of bottom plate 1 is fixedly connected with shell 2, the inner side wall upper portion of shell 2 is fixedly connected with tooth plate 4, the below of tooth plate 4 is provided with movable rack 17, the above of tooth plate 4 is provided with horizontal shaft 21, the side wall of horizontal shaft 21 is fixedly sleeved with gear 22 and worm wheel 23, gear 22 corresponds with tooth plate 4 and is engaged, one side of movable rack 17 is provided with motor 24, the output shaft end of motor 24 is fixedly connected with worm 25, worm 25 is engaged with worm wheel 23, the bottom surface middle portion of movable rack 17 is provided with electric push rod 26, the output shaft end of electric push rod 26 is fixedly connected with piston 27, the below of movable rack 17 is provided with conical barrel 28, piston 27 is located in the barrel mouth of conical barrel 28, the inner bottom wall of shell 2 is fixedly connected with convex plate 6, the above of convex plate 6 is detachably provided with placing rack 12, the top surface of placing rack 12 is provided with placing groove 15, and detection tube 16 can be taken out in placing groove 15.

[0018] The side wall of shell 2 is hinged with opening and closing door 3, and the opening and closing door 3 corresponds with the placing rack 12, the end surface of the placing rack 12 is fixedly connected with the convex disc 13, the bottom surface of the convex disc 13 is provided with the clamping groove 14, and the two sides of the top surface of the convex plate 6 are fixedly connected with the clamping rod 7, which corresponds with the clamping groove 14 and cooperates.

[0019] By adopting the above technical scheme, the opening and closing door 3 can be opened to take or place the placing rack 12. When installing the placing rack 12, the clamping groove 14 corresponds with the clamping rod 7, and the placing rack 12 is pressed downward, so that the clamping groove 14 cooperates with the clamping rod 7 to complete the installation of the placing rack 12. When disassembling, only need to operate in reverse, the placing rack 12 can be removed, and the detection tube 16 can be taken out without contacting the detection tube 16 throughout the process, avoiding pollution to the sample.

[0020] The two sides of the tooth plate 4 are fixedly connected with the sliding rail 5, the top surface of the movable rack 17 is fixedly connected with the sliding block 18, the sliding block 18 includes the sliding groove 19, the sliding groove 19 corresponds with the sliding rail 5 and is slidingly connected, the two ends of the side wall of the horizontal shaft 21 are rotatably connected with the vertical plate 20, and the bottom surface of the vertical plate 20 is fixedly connected with the top surface of the sliding block 18.

[0021] By adopting the above technical scheme, the motor 24 is started, the worm 25 rotates under the action of the output shaft of the motor 24, the horizontal shaft 21 drives the gear 22 to rotate through the mutual engagement of the worm 25 and the worm wheel 23, the movable rack 17 translates through the mutual engagement of the gear 22 and the tooth plate 4 and the cooperation of the sliding rail 5 and the sliding groove 19, and the position of the conical barrel 28 can be adjusted to make the conical barrel 28 drip reagent to different detection tubes 16.

[0022] The side wall of the motor 24 is provided with a base fixedly connected with the side wall of the movable frame 17, the side wall of the electric push rod 26 is provided with a fixed seat fixedly connected with the bottom surface of the movable frame 17, the outer side wall of the conical barrel 28 is fixedly connected with a fixed rod 29, and the top end of the fixed rod 29 is fixedly connected with the bottom surface of the movable frame 17.

[0023] By adopting the above technical scheme, the motor 24 is installed through the base, the electric push rod 26 is started, and the piston 27 moves under the action of the output shaft of the electric push rod 26, so that the liquid in the conical barrel 28 can be pushed down, and the reagent in the conical barrel 28 can be dropped.

[0024] The top surface of the convex plate 6 is fixedly connected with a convex seat 8, the top surface of the convex seat 8 is embedded with an electric cylinder 9, the output shaft end of the electric cylinder 9 is fixedly connected with a lifting disc 10, and the lifting disc 10 is placed with a reagent cup 11.

[0025] By adopting the above technical scheme, the electric cylinder 9 is started, the lifting disc 10 can drive the reagent cup 11 to ascend and descend under the action of the output shaft of the electric cylinder 9, when the reagent needs to be added to the conical barrel 28, the conical barrel 28 corresponds to the reagent cup 11, the lifting disc 10 drives the reagent cup 11 to ascend, so that the conical barrel 28 cooperates with the reagent cup 11, the piston 27 is controlled to ascend, and the reagent in the reagent cup 11 can be sucked into the conical barrel 28, after completion, the lifting disc 10 drives the reagent cup 11 to descend, and the movement of the conical barrel 28 can be avoided.

[0026] Working principle: when the utility model is used, the opening and closing door 3 is opened, the placing frame 12 with the detection tube 16 is placed in the shell 2, the clamping groove 14 corresponds to the clamping rod 7, the placing frame 12 is pressed downwards, the clamping groove 14 cooperates with the clamping rod 7, and the installation of the placing frame 12 can be completed.

[0027] Then, the motor 24 is started, the worm 25 rotates under the action of the output shaft of the motor 24, the horizontal shaft 21 drives the gear 22 to rotate through the mutual meshing of the worm 25 and the worm gear 23, the gear 22 and the toothed plate 4 are meshed, and the movable frame 17 can be translated under the cooperation of the sliding rail 5 and the sliding groove 19, so that the position of the conical barrel 28 can be adjusted, then the electric push rod 26 is started, the piston 27 moves under the action of the output shaft of the electric push rod 26, so that the liquid in the conical barrel 28 can be pushed down, and the reagent in the conical barrel 28 can be dropped to drip irrigation reagent to different detection tubes 16.

[0028] When the reagent needs to be added to the conical barrel 28, the conical barrel 28 is corresponded to the reagent cup 11, the reagent cup 11 is driven to ascend by the lifting disc 10, the conical barrel 28 is matched with the reagent cup 11, the piston 27 is controlled to ascend, the reagent in the reagent cup 11 can be sucked into the conical barrel 28, after the completion, the reagent cup 11 is driven to descend by the lifting disc 10, the movement of the conical barrel 28 can be avoided;

[0029] When the detection tube 16 needs to be taken out, the placing rack 12 is pulled upward, the clamping rod 7 is separated from the clamping groove 14, the placing rack 12 can be taken off, the detection tube 16 can be taken out, the whole process does not need to contact the detection tube 16, the pollution to the sample is avoided.

[0030] Obviously, those skilled in the art can make various modifications and variations to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application belong to the scope of the claims of the present application and the equivalent technologies thereof, the present application also intends to include these modifications and variations.

Claims

1. A constant temperature fluorescent quantitative reagent detection auxiliary device comprising a base plate (1), characterized in that: The top surface of the bottom plate (1) is fixedly connected with a shell (2), the inner side wall upper portion of the shell (2) is fixedly connected with a toothed plate (4), the lower portion of the toothed plate (4) is provided with a movable frame (17), the upper portion of the toothed plate (4) is provided with a horizontal shaft (21), the side wall of the horizontal shaft (21) is fixedly sleeved with a gear (22) and a worm wheel (23), the gear (22) corresponds and engages with the toothed plate (4), one side of the movable frame (17) is provided with a motor (24), the output shaft end of the motor (24) is fixedly connected with a worm (25), the worm (25) engages with the worm wheel (23), the bottom surface middle portion of the movable frame (17) is provided with an electric push rod (26), the output shaft end of the electric push rod (26) is fixedly connected with a piston (27), the lower portion of the movable frame (17) is provided with a conical barrel (28), the piston (27) is located in the barrel mouth of the conical barrel (28), the inner bottom wall of the shell (2) is fixedly connected with a convex plate (6), the upper portion of the convex plate (6) is detachably provided with a placing frame (12), the top surface of the placing frame (12) is provided with a placing groove (15), and the detection tube (16) can be taken out in the placing groove (15).

2. The constant temperature fluorescent quantitative reagent detection auxiliary device according to claim 1, characterized in that, The side wall of the shell (2) is hingedly connected with an opening and closing door (3), and the opening and closing door (3) corresponds to the placing frame (12).

3. The constant temperature fluorescent quantitative reagent detection auxiliary device according to claim 1, characterized in that, Both side surfaces of the toothed plate (4) are fixedly connected with slide rails (5), the top surface of the movable frame (17) is fixedly connected with a sliding block (18), the sliding block (18) comprises a sliding groove (19), and the sliding groove (19) corresponds and slidably connects with the slide rail (5).

4. The constant temperature fluorescent quantitative reagent detection auxiliary device according to claim 3, characterized in that, Both ends of the side wall of the horizontal shaft (21) are rotatably connected with vertical plates (20), and the bottom surface of the vertical plate (20) is fixedly connected with the top surface of the sliding block (18).

5. The constant temperature fluorescent quantitative reagent detection auxiliary device according to claim 1, characterized in that, The side wall of the motor (24) is provided with a base, and the base is fixedly connected with the side wall of the movable frame (17).

6. The constant temperature fluorescent quantitative reagent detection auxiliary device according to claim 1, characterized in that, The side wall of the electric push rod (26) is provided with a fixing seat, the fixing seat is fixedly connected with the bottom surface of the movable frame (17), the outer side wall of the conical barrel (28) is fixedly connected with a fixing rod (29), and the top end of the fixing rod (29) is fixedly connected with the bottom surface of the movable frame (17).

7. The constant temperature fluorescent quantitative reagent detection auxiliary device according to claim 1, characterized in that, The end surface of the placing frame (12) is fixedly connected with a convex disc (13), the bottom surface of the convex disc (13) is provided with a clamping groove (14), both sides of the top surface of the convex plate (6) are fixedly connected with clamping rods (7), the clamping rods (7) correspond and cooperate with the clamping grooves (14).

8. The constant temperature fluorescent quantitative reagent detection auxiliary device according to claim 1, characterized in that, One side of the top surface of the convex plate (6) is fixedly connected with a convex seat (8), the top surface of the convex seat (8) is inlaidly provided with an electric cylinder (9), the output shaft end of the electric cylinder (9) is fixedly connected with a lifting disc (10), and the reagent cup (11) is placed on the top surface of the lifting disc (10).

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